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effect of inactivation by sunlight on the larvicidal activities of mosquitocidal bacillus thuringiensis h-14 isolates from nigerian soils.bacillus thuringiensis h-14 is a well recognized bioinsecticide against mosquitoes. this study investigates the inactivation effect of sunlight on the larvicidal activities of four nigerian isolates of b. thuringiensis h-14, code-named obg1, obg8, gsc3 and gna13 as compared with a standard mosquito larvicide, b. thuringiensis var. israelensis (bti). a 0.125 mg/1 suspension of spore-delta-endotoxin powder of each isolate was exposed to direct sunlight for 7 h on a concrete roof (ca. 13 m high). a ...19968810143
pcr-based identification of bacillus thuringiensis isolated from soil samples in nigeria.six isolates of bacillus thuringiensis isolated from soil samples confirmed to be toxic to mosquito larvae were differentiated using a pcr-based technique. three of these isolates initially identified using a serological technique were further differentiated with the pcr amplification of the delta-endotoxin target sequences. using the total dna of isolates as template, at least four isolates yielded amplicons one or all the crystal protein genes, cryi a, b, c, or ii with sizes ranging from 238-1 ...200011204207
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