| cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in henan. | to evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ... | 2009 | 19732142 |
| [investigation of etiology of massive infection with porcine pseudorabies virus in henan and neighboring provinces]. | in early 2011, the serious outbreak of porcine pseudorabies virus (prv) infection suddenly recurred in henan and neighboring provinces. to investigate the etiology of massive infection with prv, 16 800 serum samples, 905 porcine epidemic diarrhea virus (pedv) back-feeding tissues, and 56 pr gene deleted live vaccines were colleted from january 2011 to may 2013 to detect prv field infection using a prv ge antibody test kit. the ge and tk genes of 11 new epidemic prv strains were sequenced by pcr, ... | 2014 | 25272601 |
| molecular epidemiology of pseudorabies virus in yunnan and the sequence analysis of its gd gene. | outbreaks of pseudorabies (prs) have occurred in yunnan, china, which caused significant economic loss. to determine the prevalence and origin of pr in yunnan, especially among vaccinated pigs, overall 791 samples of blood, tissue, semen, and sera were analyzed by serological methods, pcr, and sequence analysis of gd gene. detection with viral gi antibody or pcr showed that the yearly positive rates of pr virus (prv) in yunnan from 2010 to 2014 were 48.15, 21.26, 2.17, 5.22, and 0.35%, respectiv ... | 2017 | 28130636 |