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[analysis of bulk milk samples using polymerase chain reaction: an additional tool for bovine viral diarrhea monitoring].programmes for the eradication and control of infections with bovine viral diarrhea virus (bvdv) concentrate on the identification and elimination of persistently infected (pi) animals. the identification of these animals is mainly based on the detection of viral antigen using elisa techniques. protocols detecting viral nucleic acid using rt-pcr have been described recently. due to high costs the german model recommends screening of animals of 9 up to 36 months of age. screening of bulk milk sam ...200515900676
bovine viral diarrhoea eradication and control programmes in europe.the economic impact of bvdv infections has led a number of countries in europe to start eradication or control programmes. while in both cases the primary step is identification and elimination of persistently infected (pi) animals, the strategy applied thereafter is dependent on the density and seroprevalence of the regional cattle population. one of the first countries to design and implement an eradication programme was sweden in 1993, a country with a relatively low cattle density and no vac ...200312770541
antibody prevalence of hog cholera, bovine viral diarrhoea and aujeszky's disease virus in wild boars in northern germany.during the hunting season 1990/1991 a total of 841 blood samples was collected from shot wild boar corresponding to about 2.11% of the total hunting bag in lower saxony. all the sera were screened for neutralizing antibodies (nab) to hog cholera virus (hcv) and bovine viral diarrhoea virus (bvdv) by direct neutralizing peroxidase linked antibody (npla) assay. for the detection of antibodies (ab) against hcv a complex trapping blocking (ctb) elisa was used. cytotoxic sera were retested using an i ...19938404524
prevalence of genotypes 1 and 2 of bovine viral diarrhea virus in lower saxony, germany.the aim of this study was to find whether an antigenic drift had occurred in lower saxony in the past 40 years. for this, the genetic diversity of bovine viral diarrhea virus (bvdv) isolates mainly from lower saxony was estimated by rt-pcr and sequencing of a 420 bp fragment of the e2 glycoprotein gene. sixty-one field virus isolates collected during routine diagnostics between 1960 and 2000 in lower saxony, northern germany, were analyzed. phylogenetic analysis allowed discrimination of genotyp ...200111376844
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