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development and application of a fecal antigen diagnostic sandwich elisa for estimating prevalence of fasciola gigantica in cattle in central java, indonesia.the purpose of this study was to compare the sensitivity and specificity of an elisa test to detect fasciola gigantica antigens (coproantigens) in bovine feces, with fecal egg counting and an elisa for detecting anti-f. gigantica antibodies in serum. monoclonal antibodies to cathepsin l were generated and used to capture this antigen in feces of infected cattle. blood, feces, and livers were collected from 150 cattle at an abattoir in jakarta, indonesia, for anti-fasciola antibodies, coproantige ...200918763850
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