Publications

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isolation of yersinia enterocolitica from surface and well waters in ontario.yersinia enterocolitica was isolated from forty-three surface- and well-water samples and one municipal supply. in many cases these samples were negative for coliform bacteria. after incubation of membrane filters on m-endo agar les at 35 degrees c for total coliform analysis, filters were held an additional 24 h at room temperature. this method was more productive for isolation of waterborne types of y. enterocolitica than was cold enrichment in buffer alone or in combination with modified rapp ...1978709433
microbiological identification and analysis of swine tonsils collected from carcasses at slaughter.the primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine at slaughter. additional objectives were to determine if sampling the carcasses of normal or abnormal hogs provided different microbiological profiles and if the slaughter plant provides a feasible sampling frame and environment for detecting and monitoring important pathogens in tonsils that have health implications for both swine and humans. a total of 395 ...201121731180
the detection of yersinia enterocolitica in surface water by quantitative pcr amplification of the ail and yada genes.yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. pcr-based methods have been used to detect y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. in this study, quantitative pcr (qpcr)-based methods targeting the yersinia virulence genes ail and yada were used to survey the grand river watershed in southern ontario, canada. initial testing of reference strains showed that ail a ...201020375478
occurrence of salmonella, campylobacter, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes in swine.the objective of this study was to investigate the occurrence of major bacterial foodborne pathogens in swine. in total, 359 samples from manure storage tanks (91) and fresh pooled faeces (268) obtained from finisher (110), sows (78) and weanlings (80) were collected and tested. campylobacter, salmonella, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes were isolated from 36.5%, 31.5%, 5.8%, 3.3% and 3.3% of samples respectively. all e. coli o157 isolates found on 10 far ...201019638163
evaluation of a one-step biochemical screening test to determine pathogenic strains of yersinia enterocolitica. 200312756812
yersinia enterocolitica and yersinia pseudotuberculosis from wildlife in ontario. 19744612176
yersinia enterocolitica isolated from throats of swine in eastern and western canada.examination of the throat flora from swine in ontario for yersinia enterocolitica found the incidence of serotype o:3 to vary from 20% from tonsils to 50% for throat swabs and 55% for tongues. in contrast, there were no isolations of serotype o:3 from throat swabs taken from swine in the western provinces of british columbia and alberta. this incidence of serotype o:3 in swine correlates well with the human incidence of the same serotype which is 81% for all human isolations of y. enterocolitica ...19817332882
frequency of escherichia coli o157:h7 isolation from stool specimens.during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ...19873319115
canadian experience with yersinia enterocolitica (1966--1977).data pertaining to 1,219 cultures of yersinia enterocolitica isolated in canada are summarized. of the 977 cultures isolated from humans, y. enterocolitica serotype 0:3, biotype 4, phage type ixb was the most predominant type in ontario, quebec and the four eastern provinces. in the western provinces the predominant strains were indol-positive, serotypes 0:8; 5,27 and 4,32. most of the 242 cultures of nonhuman origin, except those isolated from swine and a few isolated from wild animals, were in ...1979535372
an evaluation of methods for the isolation of yersinia enterocolitica from surface waters in the grand river watershed.yersinia enterocolitica is a foodborne pathogen, but the importance of water as a route of exposure for human infection is not well known. y. enterocolitica isolation methods were developed primarily for food and clinical samples, and may not be effective for use with environmental samples. the objective of this study was to assess the recovery of y. enterocolitica from surface water used for drinking water treatment. four enrichment broths and an alkaline treatment protocol were compared for th ...200919491491
prevalence of yersinia enterocolitica shedding and bioserotype distribution in ontario finisher pig herds in 2001, 2002, and 2004.we investigated characteristics of yersinia enterocolitica infection in ontario finisher pig herds. our specific objectives were to estimate or test: prevalence of y. enterocolitica shedding in finisher pigs, bioserotype distribution, agreement between the herd-level tests based on sampling pig and pooled fecal samples, whether bioserotypes cluster by farms, and whether y. enterocolitica-positive herds cluster spatially. in total, 3747 fecal samples were collected from 100 farms over the years 2 ...201019926152
survey on the incidence of yersinia enterocolitica in the province of ontario. 19734586389
survey on the incidence of yersinia enterocolitica infection in canada.data pertaining to 278 cultures of yersinia enterocolitica isolated in canada are summarized in this paper. of this amount, 256 were isolated from humans, whereas 22 were of nonhuman sources. the typing of these cultures is presented together with their geographical location. y. enterocolitica serotype o:3 biotype 4, phage type 9b, was practically the only serotype present in the province of quebec. this serotype o:3 was also predominant in ontario, followed by serotypes o:5,27 and o:6,30; other ...19744472776
association of yersinia enterocolitica with the manufacture of cheese and occurrence in pasteurized milk.raw milk in southern ontario frequently contains yersinia enterocolitica. the potential for transmission of this organism by cheese manufactured from unpasteurized milk was evaluated by examination of milk and cheese curd samples from cheese manufacturing plants and finished cheddar and italian cheeses. the incidence of y. enterocolitica was lower in cheese curd samples (9.2%) than in raw milk (18.2%). most of the curd samples showed a positive phosphatase test, indicating production from raw mi ...1978211937
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