fluorescent amplified fragment length polymorphism analysis of norwegian bacillus cereus and bacillus thuringiensis soil isolates.we examined 154 norwegian b. cereus and b. thuringiensis soil isolates (collected from five different locations), 8 b. cereus and 2 b. thuringiensis reference strains, and 2 bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (aflp). we employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fragments in an automated fashion. no method is free of error, and we identified the major sources so tha ...200111571195
amplified fragment length polymorphism (aflp) analysis of clostridium novyi, c. perfringens and bacillus cereus isolated from injecting drug users during part of the follow-up investigations associated with an outbreak of severe illness and death among illegal injecting drug users during 2000, 43 cultures of clostridium novyi type a, 40 c. perfringens type a and 6 isolates of bacillus cereus were characterised by amplified fragment length polymorphism (aflp) analysis. among the 43 c. novyi isolates, 23 different aflp profiles were detected. the same aflp profile was detected in isolates from 18 drug users investigated during 2000 from scotland ...200212448684
determination and quantification of the emetic toxin cereulide from bacillus cereus in pasta, rice and cream with liquid chromatography-tandem mass spectrometry.a rapid and sensitive method has been developed for determination and quantification of cereulide in cream, rice and pasta. samples are homogenised after addition of amylase to cooked rice and pasta, and cereulide is extracted with methanol. after the removal of water with methyl-tert butyl ether/hexane and evaporation until dryness, no further purification was required before analysis with liquid chromatography-tandem mass spectrometry (lc-ms/ms). recently, both cereulide and (13)c6-cereulide h ...201525794364
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