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didelphis marsupialis: reservoir of babesia brasiliensis in the valley of caracas (venezuela). 19911843558
risk of trypanosoma cruzi i (kinetoplastida: trypanosomatidae) transmission by panstrongylus geniculatus (hemiptera: reduviidae) in caracas (metropolitan district) and neighboring states, venezuela.the collection of panstrongylus geniculatus bugs by inhabitants of dwellings in caracas city (metropolitan district) and in the neighboring miranda and vargas sates, venezuela, allowed for the gathering of data on the potential role of this sylvatic triatomine bug as a vector of chagas disease in this area. the natural infection by trypanosoma cruzi was recorded by examining fresh and stained faeces of the bugs. additionally, a random amplification of polymorphic dna technique for parasite ident ...200516019006
[Morphometric analysis of Panstrongylus geniculatus (Heteroptera: Reduviidae) from Caracas City, Venezuela].Introduction. In recent years, the assassin bug, Panstrongylus geniculatus, has been found infected with Trypanosoma cruzi in rural and urban areas of Caracas, Venezuela. Although historically this insect has been considered a forest species, it has become adapted to more urban artificial environments. Objective. The presence of sexual dimorphism was determined as an indicator of adaptation to domicilies. Material and methods. By Generalized Procrustes Analysis (GPA) and Elliptical Fourier Anal ...201122159489
large urban outbreak of orally acquired acute chagas disease at a school in caracas, venezuela.trypanosoma cruzi oral transmission is possible through food contamination by vector's feces. little is known about the epidemiology and clinical features of microepidemics of orally acquired acute chagas disease (cd).201020307205
[a confirmatory diagnosis of antibodies anti-trypanosoma cruzi in donors referred by blood banks in venezuela].to establish the confirmatory diagnosis of trypanosoma cruzi infection, at least two immunoserological tests (elisa, indirect hamaglutination, ih, complement fixation test, cft) were carried out in 254 donors, from public and private blood banks of venezuela, during 48 months between 1997-1998 and 2003-2004, referred to the immunology section of the tropical medicine institute in caracas. antibodies anti-t. cruzi were detected in 129/254 (50,79%) by elisa-igg or ih and cft. the "artificial xenod ...200818717262
synanthropic rodent reservoirs of trypanosoma (schizotrypanum) cruzi in the valley of caracas, venezuela.direct blood examination and xenodiagnosis of 47 synanthropic rodents (rattus rattus, r. norvegicus, mus musculus) captured in the valley of caracas, venezuela, revealed trypanosomal infections in 12 r. rattus, 10 with t. lewisi and 2 with t. cruzi. of the latter the course of parasitemia, the pleomorphism of the bloodstream trypomastigotes, tissue in naturally and experimentally infected rats and mice, host mortality, morphology of fecal parasites in rhodnius prolixus used for xenodiagnosis, an ...19979661306
trypanosoma (schizotrypanum) cruzi: histopathology in mice infected with strains isolated from didelphis marsupialis from the valley of caracas (venezuela).the histopathological alterations produced in nmri strain mice by isolates of trypanosoma cruzi from didelphis marsupialis captured near human dwellings in the valley of caracas, venezuela are described. the donor opossums showed pseudocysts and amastigotes and trypomastigotes only in the heart muscle, and few areas of discrete inflammations and lysis of some muscle cells. mice were parasitized in the heart, skeletal muscle, jejunum, colon, liver, lung, urinary bladder, penis, seminal vesicle, p ...19969460250
trypanosoma cruzi in the anal glands of urban opossums. i--isolation and experimental infections.opossums (didelphis marsupialis) captured in intensely urbanized areas of the city of caracas, venezuela, were found infected with trypanosoma cruzi. the developmental cycle of trypomastigote-epimastigote-metacyclic infective trypomastigote, usually occurring in the intestine of the triatomine vector, was taking place in the anal odoriferous glands of the opossums. material from the glands, inoculated in young, healthy opossums and white mice by different routes, subcutaneously, intraperitoneall ...19969070398
natural antibodies to human t-cell leukemia/lymphoma virus in healthy venezuelan populations.serum samples of 769 healthy venezuelan donors were assayed for natural antibodies to htlv-i by the elisa technique. specific htlv-i antibody prevalence was 6.8% but varied from 1% in caracas to 13.7% in the amazonas region and the state of zulia. adults infected with trypanosoma cruzi had the highest htlv-i antibody prevalence of 15%. areas of high antibody prevalence were correlated most strongly with the presence of arthropod-borne diseases and to a lesser extent with socio-economic factors. ...19846092282
urban trypanosoma (schizotrypanum) cruzi: pathology in white mice of isolates from panstrongylus geniculatus.the histopathological alterations produced in white mice (nmri strain) by isolates of trypanosoma (schizotrypanum) cruzi from panstrongylus geniculatus captured in dwellings of the city of caracas, venezuela are reported. the heart, skeletal muscle, liver, spleen, duodenum, colon, lung, and brain were parasitized by all the isolates. all showed strong myotropism, with elevated virulence and severe histopathological alterations in the cardiac and skeletal muscle, and in the smooth muscle of the d ...19892517002
didelphis marsupialis: a primary reservoir of trypanosoma cruzi in urban areas of caracas, venezuela.direct blood examination and xenodiagnosis of 45 sylvatic, peridomestic or domestic mammals from the caracas valley, venezuela, revealed trypanosome infection in six of the 24 opossums, didelphis marsupialis, collected in urban areas. isolates were successfully made of trypanosomes from four of the opossums, using the parasites which developed in rhodnius prolixus fed on the infected opossums to infect nmri mice. the prepatent period, course of parasitaemia, morphology of bloodstream trypomastig ...19921304702
the performance of laboratory tests in the management of a large outbreak of orally transmitted chagas disease.orally transmitted chagas disease (chd), which is a well-known entity in the brazilian amazon region, was first documented in venezuela in december 2007, when 103 people attending an urban public school in caracas became infected by ingesting juice that was contaminated with trypanosoma cruzi. the infection occurred 45-50 days prior to the initiation of the sampling performed in the current study. parasitological methods were used to diagnose the first nine symptomatic patients; t. cruzi was fou ...201223147145
update on oral chagas disease outbreaks in venezuela: epidemiological, clinical and diagnostic approaches.orally transmitted chagas disease has become a matter of concern due to outbreaks reported in four latin american countries. although several mechanisms for orally transmitted chagas disease transmission have been proposed, food and beverages contaminated with whole infected triatomines or their faeces, which contain metacyclic trypomastigotes of trypanosoma cruzi, seems to be the primary vehicle. in 2007, the first recognised outbreak of orally transmitted chagas disease occurred in venezuela a ...201525946155
panstrongylus geniculatus and four other species of triatomine bug involved in the trypanosoma cruzi enzootic cycle: high risk factors for chagas' disease transmission in the metropolitan district of caracas, venezuela.chagas' disease is caused by the protozoan trypanosoma cruzi and is autochthonous to the americas. its distribution depends on triatomine bugs that are responsible for the transmission of the disease. in 2005, we reported the presence of panstrongylus geniculatus as a risk for chagas' disease transmission in caracas and neighboring areas. three massive oral outbreaks occurred in the following years. here we report the results of a 7-year study on triatomine species found in the metropolitan dist ...201425532708
geographical distribution of trypanosoma cruzi genotypes in venezuela.chagas disease is an endemic zoonosis native to the americas and is caused by the kinetoplastid protozoan parasite trypanosoma cruzi. the parasite is also highly genetically diverse, with six discrete typing units (dtus) reported tci - tcvi. these dtus broadly correlate with several epidemiogical, ecological and pathological features of chagas disease. in this manuscript we report the most comprehensive evaluation to date of the genetic diversity of t. cruzi in venezuela. the dataset includes 77 ...201222745843
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