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the identification of two main-groups of trypanosoma cruzi stocks from brazil by their isoenzyme patterns of isoelectrofocusing.the isoelectrofocusing of seven enzymes was studied using extracts of 40 brazilian stocks of t. cruzi culture forms. four enzymes showed intraspecific variation in enzyme profiles and were selected for characterization of t. cruzi. these enzymes are: nonspecific esterase (e.c.3.1.1), phosphoglucomutase (e.c. 2.7.5.1), glucose-6-phosphate dehydrogenase (e.c. 1.1.1.49) and glucosephosphate isomerase (e.c. 5.3.1.9). the parasite stocks were isolated from endemic areas in the states: rio grande do s ...19826215750
[chagas' disease seroprevalence among school-age children in espírito santo state, brazil, 1999-2000].although the brazilian state of espírito santo is not considered endemic for chagas' disease, the sylvatic triatomines occurring there frequently invade houses, increasing the chances of trypanosoma cruzi transmission to man. the epidemiological pattern of the disease in espírito santo was evaluated by a serological survey of 5,243 schoolchildren ages 7 to 14 years, residents of 17 municipalities. indirect immunofluorescence, indirect hemagglutination, and immunoenzymatic (elisa) tests were posi ...200212488904
peridomiciliary colonies of triatoma vitticeps (stal, 1859) (hemiptera, reduviidae, triatominae) infected with trypanosoma cruzi in rural areas of the state of espírito santo, brazil.in brazil, the colonization of human dwellings by triatomines occurs in areas with native vegetation of the caatinga or cerrado types. in areas of atlantic forest such as in the brazilian state of espírito santo, there are no species adapted to live in human habitations. the few autochthonous cases of chagas disease encountered in espírito santo have been attributed to adult specimens of triatoma vitticeps that invade houses from forest remnants. in recent years, the entomology unit of the espír ...200516184222
[natural infection of triatoma vitticeps (stal, 1859) with flagellates morphologically similar to trypanosoma cruzi (chagas, 1909) in espírito santo state].adult specimens of triatoma vitticeps are frequently captured by residents in rural areas of the state of espírito santo, brazil. with aim of determining the natural infection rate of this species, we examined the excrement of 116 wild specimens, captured in 27 municipal districts of the state, after blood meal in chicken and spontaneous excretion. of these, 100 (86.2%) were infected with flagellates morphologically similar to trypanosoma cruzi. our results showed natural infection rates of trit ...200616501776
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