| the palm tree copernicia cerifera (carnaúba) as an ecotope of rhodnius nasutus in rural areas of the state of piauí, northeastern brazil. | | 1986 | 3334280 |
| [the national survey of seroprevalence for evaluation of the control of chagas disease in brazil (2001-2008)]. | a survey for seroprevalence of chagas disease was held in a representative sample of brazilian individuals up to 5 years of age in all the rural areas of brazil, with the single exception of rio de janeiro state. blood on filter paper was collected from 104,954 children and screened in a single laboratory with two serological tests: indirect immunofluorescence and enzyme linked immunoassay. all samples with positive or indetermined results, as well as 10% of all the negative samples were submitt ... | 2011 | 21584364 |
| new perspectives for population genetics of chagas'disease vectors in the northeastern brazil: isolation of polymorphic microsatellite markers in triatoma brasiliensis. | triatoma brasiliensis is the most important chagas disease vector in semiarid areas of northeastern brazil. although generally found in natural environment, it regularly colonizes or reinvades domiciliary ecotopes. in order to analyse gene flow between habitats, we identified and characterized six microsatellite loci using a microsatellite-enriched genomic library. to assess the usefulness of these microsatellites for genetic studies, we investigated their variability in two natural populations ... | 2009 | 19460330 |
| trypanosoma cruzi infection in wild mammals of the national park 'serra da capivara' and its surroundings (piaui, brazil), an area endemic for chagas disease. | we studied the prevalence of trypanosoma cruzi infection among eight species of wild small mammals (n=289) in an area where human cases of infection/disease have occurred. dogs (n=52) and goats (n=56) were also surveyed. the study was carried out inside a biological reserve, the national park 'serra da capivara' and its surroundings in piaui state, brazil. the marsupial didelphis albiventris and the caviomorph rodent trichomys apereoides were found to be the most important reservoirs in the stud ... | 2005 | 15780345 |
| [study of the infection and morbidity of chagas' disease in municipality of joão costa: national park serra da capivara, piauí, brazil]. | in order to investigate aspects of the infection and morbidity of chagas' disease in the municipality of joão costa, piauí state, brazil, we carried out a serological survey to detect anti-trypanosoma cruzi antibodies in 2,080 individuals, by indirect immunofluorescence, indirect hemagglutination and elisa. a total of 189 seropositive and 141 seronegative patients were evaluated by anamnesis, physical exam and electrocardiogram (ekg). the parasitaemia of 106 chagasic patients was evaluated by in ... | 2002 | 12170326 |
| [use of random amplified polymorphic dna (rapd) in the populational study of triatoma brasiliensis neiva, 1911]. | we evaluated the genetic variability of triatoma brasiliensis, the main vector of chagas disease in northeast brazil, using specimens from three populations. regions of genomic dna were amplified by rapd (random amplified polimorphic dna), using two primers. the products were visualized after polyacrylamide gel electrophoresis followed by silver staining. a dendrogram constructed through the dice similarity coefficient allowed for separation of the tested specimens into three distinct groups. th ... | 2000 | 11119326 |
| [epidemiology of chagas disease in the municipalities of castelo do piaui and pedro ii, state of piaui, brazil]. | triatoma brasiliensis, triatoma pseudomaculata and rhodnius nasutus were captured in artificial and natural echotopes in ten localities situated in rural areas of castelo do piauí and pedro ii, state of piauí, brazil. nymphal instars predominated and birds were the main blood meal source for triatomines. t. brasiliensis was the only triatomine captured inside the houses searched, harbouring flagellates morphologically and biologically indistinguishable from trypanosoma (schizotrypanum) cruzi. sp ... | 1989 | 2517851 |
| epidemiological survey of trypanosoma cruzi infection in north-eastern brazil using different diagnostic methods. | a survey of the prevalence of trypanosoma cruzi infection was carried out in oitis, a small community in the state of piaui, brazil. two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (ifa), enzyme-linked immunosorbent assay (elisa), and competitive enzyme-linked immunosorbent assay (c-elisa) using the monoclonal antibody tcf87 against to a 25kd t. cruzi antigen. seropositivity was 14.3% by the ifa test, 14.7% by elisa, and 1 ... | 1991 | 1844534 |
| [the epidemiology of chagas' disease in a rural area of the city of teresina, piauí, brazil]. | in the rural areas of teresina, 129 triatomines were captured distributed in (a) artificial ecotopes; a house with one triatoma brasiliensis, one panstrongylus geniculatus, rhodnius pictipes, and one rhodnius prolixus and in a uninhabited chicken house (7 rhodnius nasutus). (b) natural ecotopes; pahus orbignya martiana (41 rhodnius neglectus, 33 rhodnius prolixus and 41 rhodnius nasutus) and copernicia cerifera (3 rhodnius neglectus). the 22.6% of captured triatomines were infected by flagellate ... | 1992 | 1308066 |
| ecological niche modelling and differentiation between rhodnius neglectus lent, 1954 and rhodnius nasutus stål, 1859 (hemiptera: reduviidae: triatominae) in brazil. | ecological niche modelling was used to predict the potential geographical distribution of rhodnius nasutus stål and rhodnius neglectus lent, in brazil and to investigate the niche divergence between these morphologically similar triatomine species. the distribution of r. neglectus covered mainly the cerrado of central brazil, but the prediction maps also revealed its occurrence in transitional areas within the caatinga, pantanal and amazon biomes. the potential distribution of r. nasutus covered ... | 2009 | 20140378 |
| [epidemiology of chagas disease in four rural localities in jaguaruana, state of ceará: seroprevalence of infection, parasitemia and clinical characteristics]. | a cross-sectional study on chagas disease that examined the populations of four localities (n masculine = 541 inhabitants) in the municipality of jaguaruana, state of ceará, showed seroprevalence of chagas infection of 3.1%, as assessed by indirect immunofluorescence, indirect hemagglutination and elisa tests. the rate was higher among adults over 50 years old, without any difference in relation to sex. positive parasitemia was found in 11.8% (2/17) of the seropositive individuals by means of in ... | 2008 | 18853005 |
| [seroprevalence of chagas disease infection in the state of piauí, 2002]. | to evaluate the epidemiological situation of chagas disease infection in the state of piauí, brazil, and its relationships with age, gender, blood transfusion and spontaneous abortion, a serological survey was performed. a random sample of 36,399 inhabitants of rural zones was examined between august and december 2002. chagas disease infection was defined by the indirect immunofluorescence test, with identification of anti-trypanosoma cruzi antibodies in blood samples collected on filter paper. ... | 2006 | 17308697 |
| mapping of the distribution of trypanosoma cruzi infection among small wild mammals in a conservation unit and its surroundings (northeast-brazil). | maps are a useful tool that permits correlation of landscapes with hotspots of parasite transmission. here, they were used as a tool for geovisualization to evaluate variables involved in the transmission of trypanosoma cruzi among small wild mammals in an area endemic for chagas disease, the "serra da capivara" national park (parna) and its surroundings in piauí state, northeast brazil. the implementation of a geographical information system (gis) allowed the observation that a previously noted ... | 2007 | 17307027 |
| molecular epidemiology of american trypanosomiasis in brazil based on dimorphisms of rrna and mini-exon gene sequences. | american trypanosomiasis is transmitted in nature via a sylvatic cycle, where trypanosoma cruzi interacts with wild triatomines and mammalian reservoirs, or via a domestic cycle where the parasite comes into contact with humans through domiciliated triatomines. the pool of t. cruzi isolates consists of sub-populations presenting a broad genetic diversity. in contrast to the heterogeneity suggested by isoenzyme analysis, pcr amplification of sequences from the 24s alpha rrna gene and from the non ... | 1998 | 9504338 |
| trypanosoma cruzi-like bloodstream trypomastigotes in bats from the state of piauí, northeastern brazil. | | 1986 | 3317557 |
| trypanosoma cruzi: subtractive hybridization as a molecular strategy to generate new targets to distinguish groups and hybrids. | rapd analysis and sequences of the mini-exon and ribosomal genes show that trypanosoma cruzi can be clustered into two phylogenetic groups-t. cruzi i and ii. herein, the representational difference analysis (rda) method was used, providing new targets specific for each group. after three rounds of rda hybridizing f strain (tester) with y strain (driver) and vice-versa, an f-specific (f#30) and y-specific (y#22) clone were obtained specifically recognizing isolates from amazonas (t. cruzi i) and ... | 2007 | 17597613 |