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development and application of a quantitative, specific assay for cryptosporidium parvum oocyst detection in high-turbidity environmental water samples.chlorine-resistant cryptosporidium parvum oocysts in drinking water play an important role in the epidemiology of cryptosporidiosis. current methods of detecting these organisms in water are insensitive, labor-intensive, highly subjective, and severely limited by sample turbidity. we describe here an alternative technique utilizing electrochemiluminescence (ecl) technology for detecting c. parvum oocysts in environmental water samples. this method is quantitative, reproducible, and requires only ...200111504397
prevalence of cryptosporidium parvum infection in children along the texas-mexico border and associated risk factors.we examined the epidemiology of cryptosporidium parvum in children aged 6 months to 13 years living in 1) colonias along the border (n = 105), 2) a clinic in an urban border community (n = 65), and 3) clinics in a large urban nonborder area (n = 109). serum igg and iga anticryptosporidial antibodies were measured by enzyme-linked immunosorbent assay (elisa). overall, 70.2% (196/279) of subjects had detectable c. parvum antibodies. prevalence rates were higher (93/105 [89%]) in the colonias and u ...200011289680
prevalence of and risk factors for fecal shedding of cryptosporidium parvum oocysts in horses.to determine prevalence of and risk factors for fecal shedding of cryptosporidium parvum oocysts among 3 populations of horses.19989810386
environmental load of cryptosporidium parvum oocysts from cattle manure in feedlots from the central and western united states.the first step in assessing the risk of water contamination by cryptosporidium parvum oocysts from feedlot cattle (bos taurus) production systems is to quantify the number of c. parvum oocysts present in the fecal material deposited by feedlot cattle. our primary objective for this project was to estimate the daily environmental load of c. parvum oocysts in fecal material deposited by feedlot cattle from across the central and western usa. our secondary goal was to genotype isolates of c. parvum ...200616391291
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