respiratory viral infections detected by multiplex pcr among pediatric patients with lower respiratory tract infections seen at an urban hospital in delhi from 2005 to 2007.acute lower respiratory tract infections (alri) are the major cause of morbidity and mortality in young children worldwide. information on viral etiology in alri from india is limited. the aim of the present study was to develop a simple, sensitive, specific and cost effective multiplex pcr (mpcr) assay without post pcr hybridization or nested pcr steps for the detection of respiratory syncytial virus (rsv), influenza viruses, parainfluenza viruses (piv1-3) and human metapneumovirus (hmpv). naso ...200919558656
genetic variability in the g protein gene of group a and b respiratory syncytial viruses from india.respiratory syncytial virus (rsv) is the most commonly identified viral agent of acute respiratory tract infection (ari) of young children and causes repeat infections throughout life. limited data are available on the molecular epidemiology of rsv from developing countries, including india. this study reports on the genetic variability in the glycoprotein g gene among rsv isolates from india. reverse transcription-pcr for a region of the rsv g protein gene was done with nasopharyngeal aspirates ...200616954227
genetic variability among group a and b respiratory syncytial virus isolates from a large referral hospital in new delhi, india.respiratory syncytial virus (rsv) is an important childhood pathogen of acute lower respiratory infections in developed and developing countries. the molecular epidemiology of rsv in india is largely unknown. the present study was undertaken to standardize and evaluate reverse transcription-pcr (rt-pcr) for the rapid and simultaneous detection of rsv groups a and b in clinical samples and to study intragroup genetic variability. rt-pcr was evaluated by comparing the results of seminested rt-pcr ...200312791842
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