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detection and differentiation of cryptosporidium by real-time polymerase chain reaction in stool samples from patients in rio de janeiro, brazil.this study reports the first genetic characterisation of cryptosporidium isolates in brazil using real-time polymerase chain reaction (rt-pcr). a total of 1,197 faecal specimens from children and 10 specimens from human immunodeficiency virus-infected patients were collected between 1999-2010 and screened using microscopy. forty-eight cryptosporidium oocyst-positive isolates were identified and analysed using a generic taqman assay targeting the 18s rrna to detect cryptosporidium species and two ...201222666857
genetic diversity of cryptosporidium identified in clinical samples from cities in brazil and argentina.the identification and characterisation of cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. the objective was to determine the genetic diversity of cryptosporidium in samples obtained from hospitals of rio de janeiro, brazil, and buenos aires, argentina. samples were analysed by microscopy and taqman polymerase chain reaction (pcr) assays for cryptosporidium detection, genotyped by nested-pcr-restr ...201626814641
Cryptosporidium spp. parasitize exotic birds that are commercialized in markets, commercial aviaries, and pet shops.The purpose of this study was to genetically characterize and phylogenetically analyze the Cryptosporidium spp. isolated from exotic birds commercialized in popular markets, commercial aviaries, and pet shops located in Rio de Janeiro, Brazil. Fecal samples from individually housed birds were collected and subjected to centrifuge-flotation technique using saturated sugar solution. DNA was isolated from Cryptosporidium positive samples, and 18S subunit rDNA was amplified and processed using neste ...201121922240
new cryptosporidium parvum subtypes of iia subfamily in dairy calves from brazil.bovine cryptosporidiosis is mainly caused by four distinct species: cryptosporidium parvum, c. bovis, c. ryanae and c. andersoni. the first, c. parvum, is a major concern in livestock causing economic losses, in addition to public health impact because of its zoonotic characteristics. the present study aimed to determine the occurrence of different species and subtypes of cryptosporidium using molecular techniques. a total of 143 fecal samples were collected from calves from three dairy farms lo ...201424239750
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