| detection of asymptomatic antigenemia in pigs infected by porcine reproductive and respiratory syndrome virus (prrsv) by a novel capture immunoassay with monoclonal antibodies against the nucleocapsid protein of prrsv. | routine surveillance for porcine reproductive and respiratory syndrome virus (prrsv) infections is crucial for the epidemiological control of this disease. antibody tests are widely used but cannot differentiate between vaccination and reinfection. we developed a prrsv antigen capture enzyme-linked immunosorbent assay (elisa) using well-characterized monoclonal antibodies (mabs) raised against the nucleocapsid (n) protein of north american and european prrsv. this antigen assay detected purified ... | 2009 | 19828768 |
| genetic characterization of 11 porcine reproductive and respiratory syndrome virus isolates in south china from 2014 to 2015. | porcine reproductive and respiratory syndrome (prrs) has leaded to an enormous loss per year to the swine industry, its etiology porcine reproductive and respiratory syndrome virus (prrsv) is a highly mutated virus in pigs. to fully understand the genetic characteristics of prrsv genome in south china, this study collected the lung samples infected with prrsv in guangdong and hainan province from 2014 to 2015 and tried to isolate the prrsv. finally, the complete genomes of isolated strains were ... | 2017 | 28738888 |
| a new recombined prrsv virulent strain in china. | porcine reproductive and respiratory syndrome (prrs) is one of the most important swine diseases worldwide. in the present study, a new virulent strain of prrs virus(prrsv) gdsg was isolated in guangdong province of china, which caused high fever, high morbidity and high mortality for sow and piglets. the genome of this new strain is 15,413 nucleotides(nt) in length and comparative analysis revealed that gdsg shared 82.4% to 94% identity with type 2 prrsv strains, but only 61.5% with type 1 prrs ... | 2017 | 28693303 |
| sequence analysis of nsp9 gene of 25 prrsv strains from guangdong province, subtropical southern china. | in order to evaluate the trend in the prevalence of porcine reproductive and respiratory syndrome on farms, 25 porcine reproductive and respiratory syndrome virus (prrsv) strains were collected from 2009 to 2012 from 11 districts in the guangdong province. the complete gene sequences of nsp9 from the 25 prrsv strains were amplified, sequenced, and then compared with the published sequences of other strains. the results showed that these sequences shared 97.6-99.4 % of the same nucleotides and 97 ... | 2013 | 23180485 |
| genetic diversity analysis of the orf5 gene in porcine reproductive and respiratory syndrome virus samples from south china. | to understand the genetic diversity of porcine reproductive and respiratory syndrome virus (prrsv) in south china, we collected 231 clinical samples from pigs with suspected prrsv infection in guangdong between 2007 and 2009. we found that 74 of 231 samples were positive by rt-pcr. the pcr products of the orf5 gene of 35 isolates from different farms were sequenced and their dna sequences were compared to 23 other prrsv isolates in the genbank. we found that the nucleotide similarity among all s ... | 2012 | 22366140 |