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prevalence of toxoplasma gondii antibodies in wild and domestic animals in northern california.wild and domestic animals from 3 geographic-climatologic areas in northern california were tested for antibodies against toxoplasma gondii. a total of 2,796 serum samples representing 37 species of wild mammals, 35 species of wild birds, and 5 species of domestic animals were tested by the indirect hemagglutination test. of 1,174 wild mammal serums tested, 10.8% were positive, which compared with 14.7% of the 1,221 domestic mammal serums. of 229 wild carnivores tested, 45% were seropositive, inc ...1976977457
toxoplasma antibodies among bobcats and other carnivores of norther california.the prevalence of antibodies to toxoplasma gondii was investigated among five species of wild carnivores in norther ccalifornia. the highest prevalence was among bobcats (lynx rufus), with 15 of 21 tested being serologically positive. other results included serological evidence of toxoplasmosis in two of seven raccoons (procyon lotor), one of three badgers (taxidea taxus) and two of three coyotes (canis latrans). two gray foxes (urocyon cinereoargenteus) were serologically negative. oone badger ...19751142562
a survey for toxoplasma gondii antibodies in deer and other wildlife on a sheep range.blood samples were obtained from native mammals and birds on a sheep range (hopland field station) in northern california. serums were tested for antibodies to toxoplasma gondii by the indirect hemagglutination test. of 382 deer that were tested from 1964 to 1973, 77 (20%) were seropositive for t gondii. among 36 serums representing 6 species of wild carnivores (badgers, bobcats, coyotes, foxes, raccoons, and skunks), 18 (50%) were seropositive. all of the 5 bobcats tested were seropositive, wit ...19751176348
accessory suckers (?) in taenia taeniaeformis from the california bobcat. 19734711672
bartonella henselae antibody prevalence in free-ranging and captive wild felids from california.in order to determine the importance of wild felids in the epidemiology of bartonella spp. infection, 136 nobuto strips or serum samples from free-ranging mountain lions (felis concolor) and bobcats (felis rufus) captured in california (usa) between 1985 and 1996 were tested for b. henselae antibodies (titer > or = 1:64) using an immunofluorescence test. similarly, 124 serum samples from 114 captive wild cats representing 26 species or subspecies collected between 1991 and 1995 were retrieved fr ...19989476226
molecular tracking of mountain lions in the yosemite valley region in california: genetic analysis using microsatellites and faecal dna.twelve microsatellite loci were characterized in california mountain lions (puma concolor) and sufficient polymorphism was found to uniquely genotype 62 animals sampled at necropsy. microsatellite genotypes obtained using mountain lion faecal dna matched those from muscle for all of 15 individuals examined. dna from potential prey species and animals whose faeces could be misidentified as mountain lion faeces were reliably distinguished from mountain lions using this microsatellite panel. in a f ...200010736046
seroprevalence of toxoplasma gondii in american free-ranging or captive pumas (felis concolor) and bobcats (lynx rufus).toxoplasma gondii is a major zoonotic agent infecting a wide range of mammals, including wild felids. like domestic cats, wild felids are involved in the complete infective cycle of t. gondii, as they can host in their gastrointestinal tract sexually mature parasites and shed infective oocysts in their feces. in order to evaluate the importance of this wildlife reservoir, 438 serum samples collected between 1984 and 1999 from 438 pumas (felis concolor) and from 58 bobcats (lynx rufus) from north ...200415019138
seroprevalence of bartonella infection in american free-ranging and captive pumas (felis concolor) and bobcats (lynx rufus).bartonella henselae is the main agent of cat scratch disease in humans and domestic cats are the main reservoir of this bacterium. we conducted a serosurvey to investigate the role of american wild felids as a potential reservoir of bartonella species. a total of 479 samples (439 serum samples and 40 nobuto strips) collected between 1984 and 1999 from pumas (felis concolor) and 91 samples (58 serum samples and 33 nobuto strips) collected from bobcats (lynx rufus) in north america, central americ ...200415099499
exposure to feline and canine pathogens in bobcats and gray foxes in urban and rural zones of a national park in california.exposure of bobcats (lynx rufus) and gray foxes (urocyon cinereoargenteus) to a range of common canine and feline pathogens was assessed in urban and rural zones of golden gate national recreation area, a national park in the san francisco bay area, (california, usa) from 1992 to 1995. testing included serology for canine distemper virus, canine parvovirus (cpv), canine adenovirus, leptospira interrogans, feline calicivirus (fcv), feline panleukopenia virus, feline herpesvirus, feline enteric co ...200415137484
serologic evidence of west nile virus exposure in north american mesopredators.sera from 936 mammalian mesopredators (virginia opossums, gray foxes, striped skunks, hooded skunks, raccoons, a bobcat, and a red fox) were collected during 2003 and 2004 in california, arizona, texas, louisiana, ohio, and wyoming and screened for flavivirus-specific antibodies by an epitope-blocking enzyme-linked immunosorbent assay (blocking elisa). serum samples positive for antibodies against flaviviruses were screened for west nile virus (wnv)-specific antibodies by blocking elisa and sele ...200717255248
frequent transmission of immunodeficiency viruses among bobcats and pumas.with the exception of human immunodeficiency virus (hiv), which emerged in humans after cross-species transmissions of simian immunodeficiency viruses from nonhuman primates, immunodeficiency viruses of the family lentiviridae represent species-specific viruses that rarely cross species barriers to infect new hosts. among the felidae, numerous immunodeficiency-like lentiviruses have been documented, but only a few cross-species transmissions have been recorded, and these have not been perpetuate ...200717670835
type x toxoplasma gondii in a wild mussel and terrestrial carnivores from coastal california: new linkages between terrestrial mammals, runoff and toxoplasmosis of sea otters.sea otters in california are commonly infected with toxoplasma gondii. a unique type x strain is responsible for 72% of otter infections, but its prevalence in terrestrial animals and marine invertebrates inhabiting the same area was unknown. between 2000 and 2005, 45 terrestrial carnivores (lions, bobcats, domestic cats and foxes) and 1396 invertebrates (mussels, clams and worms) were screened for t. gondii using pcr and dna sequencing to determine the phylogeographic distribution of t. gondii ...200818452923
seroprevalence of trypanosoma cruzi among eleven potential reservoir species from six states across the southern united states.trypanosoma cruzi, the causative agent of chagas' disease, is a substantial public health concern in latin america. although rare in humans and domestic animals in the united states, t. cruzi is commonly detected in some wildlife species, most commonly raccoons (procyon lotor) and virginia opossums (didelphis virginiana). to increase our understanding of the reservoir host species range and geographic distribution, 11 species of mammals from six states spanning the known range of t. cruzi (arizo ...201020020815
distribution and prevalence of cytauxzoon felis in bobcats (lynx rufus), the natural reservoir, and other wild felids in thirteen states.cytauxzoon felis, a protozoan parasite of wild and domestic felids, is the causative agent of cytauxzoonosis in domestic and some exotic felids in the united states. the bobcat (lynx rufus) is the natural reservoir for this parasite, but other felids such as florida panthers (puma concolor coryii) and domestic cats may maintain long-term parasitemias and serve as reservoirs. experimentally, two tick species, dermacentor variabilis and amblyomma americanum, have demonstrated the ability to transm ...201021071149
effects of management of domestic dogs and recreation on carnivores in protected areas in northern california.in developed countries dogs (canis lupus familiaris) are permitted to accompany human visitors to many protected areas (e.g., >96% of protected lands in california, u.s.a.), and protected-area management often focuses on regulating dogs due to concerns about predation, competition, or transmission of disease and conflicts with human visitors. in 2004 and 2005, we investigated whether carnivore species richness and abundance were associated with management of domestic dogs and recreational visita ...201121309853
isolation of bartonella henselae, bartonella koehlerae subsp. koehlerae, bartonella koehlerae subsp. bothieri and a new subspecies of b. koehlerae from free-ranging lions (panthera leo) from south africa, cheetahs (acinonyx jubatus) from namibia and captive cheetahs from california.bartonellae are blood- and vector-borne gram-negative bacteria, recognized as emerging pathogens. whole-blood samples were collected from 58 free-ranging lions (panthera leo) in south africa and 17 cheetahs (acinonyx jubatus) from namibia. blood samples were also collected from 11 cheetahs (more than once for some of them) at the san diego wildlife safari park. bacteria were isolated from the blood of three (5%) lions, one (6%) namibian cheetah and eight (73%) cheetahs from california. the lion ...201627453220
pathogen exposure varies widely among sympatric populations of wild and domestic felids across the united states.understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. the relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. we screened > 1000 samples from sympatric populations of puma (puma concolor), bobcat (lynx rufus), and domestic ca ...201627209780
feline immunodeficiency virus cross-species transmission: implications for emergence of new lentiviral infections.owing to a complex history of host-parasite coevolution, lentiviruses exhibit a high degree of species specificity. given the well-documented viral archeology of human immunodeficiency virus (hiv) emergence following human exposures to simian immunodeficiency virus (siv), an understanding of processes that promote successful cross-species lentiviral transmissions is highly relevant. we previously reported natural cross-species transmission of a subtype of feline immunodeficiency virus, puma lent ...201728003486
characterization of regionally associated feline immunodeficiency virus (fiv) in bobcats (lynx rufus).feline immunodeficiency virus (fiv) classically infects felid species with highly divergent species-specific fivs. however, recent studies have detected an fiv strain infecting both bobcats (lynx rufus) and pumas (puma concolor) in california and florida. to further investigate this observation, we evaluated fiv from bobcats in florida (n=25) and colorado (n=80) between 2008 and 2011. partial viral sequences from five florida bobcats cluster with previously published sequences from florida panth ...201323778629
gene flow and pathogen transmission among bobcats (lynx rufus) in a fragmented urban landscape.urbanization can result in the fragmentation of once contiguous natural landscapes into a patchy habitat interspersed within a growing urban matrix. animals living in fragmented landscapes often have reduced movement among habitat patches because of avoidance of intervening human development, which potentially leads to both reduced gene flow and pathogen transmission between patches. mammalian carnivores with large home ranges, such as bobcats (lynx rufus), may be particularly sensitive to habit ...201222335296
zoonotic parasites of bobcats around human landscapes.we analyzed lynx rufus fecal parasites from california and colorado, hypothesizing that bobcats shed zoonotic parasites around human landscapes. giardia duodenalis, cryptosporidium, ancylostoma, uncinaria, and toxocara cati were shed. toxoplasma gondii serology demonstrated exposure. giardia and cryptosporidium shedding increased near large human populations. genotyped giardia may indicate indirect transmission with humans.201222718941
diversity of rickettsiae in a rural community in northern california.far northern california forests are highly biodiverse in wildlife reservoirs and arthropod vectors that may propagate rickettsial pathogens in nature. the proximity of small rural communities to these forests puts people and domestic animals at risk of vector-borne infection due to spillover from wildlife. the current study was conducted to document exposure to rickettsial pathogens in people and domestic animals in a rural community, and identify which rickettsiae are present in sylvatic and pe ...201728274808
prevalence and potential impact of toxoplasma gondii on the endangered amargosa vole (microtus californicus scirpensis), california, usa.we investigated the prevalence of toxoplasma gondii in 2011-15 to assess its potential threat on the endangered amargosa vole ( microtus californicus scirpensis) in california, us. surveillance was simultaneously performed on populations of syntopic rodent species. we detected antibodies to t. gondii in sera from 10.5% of 135 wild-caught amargosa voles; 8% of 95 blood samples were pcr-positive for the t. gondii b1 gene, and 5.0% of 140 sympatric rodent brain samples were pcr-positive. exposure t ...201727690194
using molecular epidemiology to track toxoplasma gondii from terrestrial carnivores to marine hosts: implications for public health and conservation.environmental transmission of the zoonotic parasite toxoplasma gondii, which is shed only by felids, poses risks to human and animal health in temperate and tropical ecosystems. atypical t. gondii genotypes have been linked to severe disease in people and the threatened population of california sea otters. to investigate land-to-sea parasite transmission, we screened 373 carnivores (feral domestic cats, mountain lions, bobcats, foxes, and coyotes) for t. gondii infection and examined the distrib ...201424874796
toxoplasma gondii, source to sea: higher contribution of domestic felids to terrestrial parasite loading despite lower infection prevalence.environmental transmission of toxoplasma gondii, a global zoonotic parasite, adversely impacts human and animal health. toxoplasma is a significant cause of mortality in threatened southern sea otters, which serve as sentinels for disease threats to people and animals in coastal environments. as wild and domestic felids are the only recognized hosts capable of shedding toxoplasma oocysts into the environment, otter infection suggests land-to-sea pathogen transmission. to assess relative contribu ...201324048652
novel gammaherpesviruses in north american domestic cats, bobcats, and pumas: identification, prevalence, and risk factors.gammaherpesviruses (ghvs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. to identify felid ghvs, we screened domestic cat (felis catus), bobcat (lynx rufus), and puma (puma concolor) blood cell dna samples from california, colorado, and florida using a degenerate pan-ghv pcr. additional pan-ghv and long-distance pcrs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glyco ...201424453374
development and validation of a fecal pcr assay for notoedres cati and application to notoedric mange cases in bobcats (lynx rufus) in northern california, usa.notoedric mange in felids is a devastating disease caused by a hypersensitivity reaction to the mite notoedres cati. the burrowing of the mite causes intense pruritis resulting in self-mutilation, secondary bacterial infection, and often death of affected felids if left untreated. our understanding of how notoedric mange is maintained in felid populations, and the true geographic extent of infestations, has been hampered because wild felids are elusive and, thus, traditional diagnostic methods a ...201323568905
mapping behavioral landscapes for animal movement: a finite mixture modeling approach.because of its role in many ecological processes, movement of animals in response to landscape features is an important subject in ecology and conservation biology. in this paper, we develop models of animal movement in relation to objects or fields in a landscape. we took a finite mixture modeling approach in which the component densities are conceptually related to different choices for movement in response to a landscape feature, and the mixing proportions are related to the probability of se ...201323734492
carnivore use of avocado orchards across an agricultural-wildland gradient.wide-ranging species cannot persist in reserves alone. consequently, there is growing interest in the conservation value of agricultural lands that separate or buffer natural areas. the value of agricultural lands for wildlife habitat and connectivity varies as a function of the crop type and landscape context, and quantifying these differences will improve our ability to manage these lands more effectively for animals. in southern california, many species are present in avocado orchards, includ ...201323844147
serum chemistry, hematologic, and post-mortem findings in free-ranging bobcats (lynx rufus) with notoedric mange.notoedric mange was responsible for a population decline of bobcats ( lynx rufus ) in 2 southern california counties from 2002-2006 and is now reported to affect bobcats in northern and southern california. with this study we document clinical laboratory and necropsy findings for bobcats with mange. bobcats in this study included free-ranging bobcats with mange (n = 34), a control group of free-ranging bobcats without mange (n = 11), and a captive control group of bobcats without mange (n = 19). ...201323957865
anticoagulant rodenticides in urban bobcats: exposure, risk factors and potential effects based on a 16-year study.anticoagulant rodenticides (ars) are increasingly recognized as a threat to nontarget wildlife. high exposure to ars has been documented globally in nontarget predatory species and linked to the high prevalence of an ectoparasitic disease, notoedric mange. in southern california, mange associated with ar exposure has been the proximate cause of a bobcat (lynx rufus) population decline. we measured ar exposure in bobcats from two areas in southern california, examining seasonal, demographic and s ...201525707484
mark-recapture and mark-resight methods for estimating abundance with remote cameras: a carnivore case study.abundance estimation of carnivore populations is difficult and has prompted the use of non-invasive detection methods, such as remotely-triggered cameras, to collect data. to analyze photo data, studies focusing on carnivores with unique pelage patterns have utilized a mark-recapture framework and studies of carnivores without unique pelage patterns have used a mark-resight framework. we compared mark-resight and mark-recapture estimation methods to estimate bobcat (lynx rufus) population sizes, ...201525822245
dental pathology of the california bobcat (lynx rufus californicus).skulls from 277 california bobcats (lynx rufus californicus) were examined macroscopically and by radiography. the majority of the skulls were from adult animals (79.8%). the skulls were from 128 male (46.2%) and 114 female (41.2%) animals and gender was unknown for the remainder. the majority (95.6%) of teeth were present for examination. only 16 teeth were identified as absent congenitally and 15 of these were incisor teeth. teeth with abnormal morphology were rare (0.5%). the two most common ...201627102444
a southern california freeway is a physical and social barrier to gene flow in carnivores.roads present formidable barriers to dispersal. we examine movements of two highly mobile carnivores across the ventura freeway near los angeles, one of the busiest highways in the united states. the two species, bobcats and coyotes, can disappear from habitats isolated and fragmented by roads, and their ability to disperse across the ventura freeway tests the limits of vertebrates to overcome anthropogenic obstacles. we combine radio-telemetry data and genetically based assignments to identify ...200616689893
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