novel genetic environment of the plasmid-mediated kpc-3 gene detected in escherichia coli and citrobacter freundii isolates from china.the imipenem and meropenem-resistant strains citrobacter freundii hs70 and escherichia coli hs510 were isolated from patients in shanghai, china. by isoelectric focusing, pcr amplification and sequencing, these strains were each found to produce four β-lactamases: tem-1, kpc-3, shv-7 and ctx-m-14. a conjugation experiment and plasmid restriction digestion revealed that the bla (kpc-3) gene was located on the same plasmid in both isolates. bidirectional primer walking sequencing showed that the n ...201021153909
detection and spread of carbapenem-resistant citrobacter freundii in a teaching hospital in china.background: we examined the detection and spread of carbapenem-resistant citrobacter freundii in huashan hospital, shanghai, china between 2005 and 2008. methods: twenty-three isolates of carbapenem-resistant c freundii collected in our hospital underwent resistant gene amplification by polymerase chain reaction, followed by minimal inhibitory concentration (mic) analysis. molecular epidemiologic analyses included pulsed-field gel electrophoresis and case study. results: analysis of mics with am ...201121705111
Emergence of carbapenem-resistant clinical Enterobacteriaceae isolates from a teaching hospital in Shanghai, China.Carbapenems such as imipenem and meropenem are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant clinical isolates of bacteria belonging to the family Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among members of the family Enterobacteriaceae isolated from a teaching hospital in Shanghai, China. In the present study, we investigated the prevalence and molecular characteristics of carbapenem-resistant clinical i ...201221903823
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