| monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription-polymerase chain reaction for odontoglossum ringspot virus detection. | odontoglossum ringspot virus (orsv) infects numerous commercially important orchids and causes significant losses worldwide. the coat protein (cp) gene of orsv was cloned and expressed in escherichia coli by using the pet-32a expression vector, and the expression of recombinant protein was confirmed by western blotting using anti-orsv antibodies. the recombinant protein was purified using ni-nta agarose, and the purified protein was used as an immunogen to produce monoclonal antibodies (mabs) an ... | 2010 | 20933014 |