| [studies on man-cattle-man infection cycle of sarcocystis hominis in yunnan]. | the present paper reports on the results of an experimental study on man-cattle-man infection of sarcocystis hominis, found in yunnan province. about ten thousand sporocysts collected from the feces of persons naturally infected with sarcocystis hominis were fed to a calf, which was dissected 150 days later. numerous cysts of sarcocystis hominis were found in the cardiac and skeletal muscles. by light microscopy, the cyst wall of fresh preparation showed numerous thick, finger-like projections, ... | 1990 | 2114230 |
| a brief introduction to a nation-wide sampling survey on schistosomiasis. | a nation-wide sampling survey using a stratified group randomized sampling technique was conducted in 1989 in 8 provinces where schistosomiasis was still endemic. among 343 sampling spots, 197 were in controlled areas and 146 in endemic areas. 16,953 (10.2%) of 165,834 persons were found infected according to stool examination in the endemic areas with a population of 1.03% of the total in china. the positive rates of stool examination in hunan, hubei, jiangxi, anhui, jiangsu, zhejiang, yunnan a ... | 1993 | 8222904 |
| application of deltamethrin-impregnated bednets for mosquito and malaria control in yunnan, china. | the results of research in china in recent years show that: 1) different laboratory sensitivity of adult anopheles minimus, an. sinensis and culex pipiens quinquefasciatus to deltamethrin exist in yunnan. although the range and duration for the use of permethrin and deltamethrin was limited, resistance of an. minimus, an. sinensis and cx. pipiens quinquefasciatus to different extent in some areas existed. 2) on walls built with cement and covered with a thin layer of lime on which deltamethrin a ... | 1996 | 9280005 |
| analysis of social factors and human behavior attributed to family distribution of schistosomiasis japonica cases. | an investigation of family distribution patterns of schistosomiasis japonica was carried out in four pilot villages in yunnan province. family clustering of the disease was present in bihuayuan village before and after economic reform (site a); however, such clustering did not occur in tuanshan and liantie villages (sites b and c) in the same periods. being contrary to the stage prior to economic reform family clustering existed in zhonghe village (site d) after the reform. the results obtained ... | 1997 | 9444007 |
| influence of livestock husbandry on schistosomiasis transmission in mountainous regions of yunnan province. | an investigation was conducted in the highly endemic areas of schistosomiasis japonica in weishan and eryuan counties, yunnan province. the results are summarized as follows: 1) the number of domestic animals was increasing annually; 2) the proportion of animal husbandry gains in the total agriculture income had a yearly escalating tendency; 3) the infection rate of inhabitants was upgrading as a result of the development of and the prevalence in domestic animals in the recent decades. owing to ... | 1997 | 9444008 |
| transmission factors of schistosomiasis japonica in the mountainous regions with type of plateau canyon and plateau basin. | to provide the scientific basis for formulating the control strategies of schistosomiasis japonica in mountainous regions in china schistosomiasis control programme. | 1997 | 9594275 |
| laboratory and field studies of an antigen capture elisa for bluetongue virus. | an improved bluetongue antigen capture elisa (btace) technique was evaluated for its ability to detect the full range of 24 bluetongue (blu) serotypes. the btace detected all 24 serotypes in cell culture fluids, including eight serotypes where the representative strains originated from both australia and also from the south african reference collection. the amount of infectious virus required to obtain a positive btace result varied between 100-1000 tcid50. this was approximately 10-fold more se ... | 2000 | 10716347 |
| identification of seven serotypes of bluetongue virus from the people's republic of china. | seven serotypes (1, 2, 3, 4, 12, 15 and 16) of bluetongue virus were isolated from the blood of sheep and cattle in the people's republic of china between 1986 and 1996. six of these viruses were isolated in yunnan province. the sheep from which serotypes 1 and 16 were isolated showed obvious signs of bluetongue disease, whereas the cattle from which serotypes 2, 3, 4, 12 and 15 were isolated were clinically normal. phylogenetic analyses of these viruses indicate that they are more closely relat ... | 1999 | 10755588 |
| vitrification of yunnan yellow cattle oocytes: work in progress. | the objective of this study was to provide a simple cryopreservation method for oocytes from yunnan yellow cattle and facilitate preservation efforts in this native chinese breed, which is threatened by agricultural modernization. cumulus-oocyte complexes (cocs) were collected from slaughterhouse ovaries and matured in vitro for 22-24 h, then selected for cryopreservation. vitrification in open pulled straws (ops) or in microdrops on a cooled metal surface (solid surface vitrification, ssv) was ... | 2002 | 12387339 |
| a pcr-based rflp analysis of sarcocystis cruzi (protozoa: sarcocystidae) in yunnan province, pr china, reveals the water buffalo (bubalus bubalis) as a natural intermediate host. | a polymerase chain reaction-based restriction fragment length polymorphism (rflp) approach is used to examine sarcocystis cruzi-like taxa from the atypical intermediate host, water buffalo, in yunnan, people's republic of china. the loci examined lie within the 18s rrna gene. a total of 15 water buffalo isolates are compared with those of 10 s. cruzi from cattle. rflp patterns for the s. cruzi isolates from cattle and the s. cruzi-like taxon from water buffalo are found to be identical with all ... | 2002 | 12537125 |
| [investigation on schistosoma japonicum infection in rodents in yunnan province]. | to investigate the role of rodents in the transmission of schistosomiasis in endemic areas in yunnan province. | 2000 | 12567670 |
| relationship of the livestock trade to schistosomiasis transmission in mountainous area. | to study the relationship between the livestock trade and schistosomiasis transmission and to provide an evidence for making a strategy of schistosomiasis control in mountainous areas. | 2000 | 12567688 |
| [investigation of toxoplasma gondii infected animals in yunnan province]. | | 2002 | 12568045 |
| genetic diversity and relationship of yunnan native cattle breeds and introduced beef cattle breeds. | in this study, random amplified polymorphic dna (rapd) analysis was used to estimate genetic diversity and relationship in 134 samples belonging to two native cattle breeds from the yunnan province of china (dehong cattle and diqing cattle) and four introduced beef cattle breeds (brahman, simmental, murrygrey, and shorthorn). ten primers were used, and a total of 84 bands were scored, of which 63 bands (75.0%) were polymorphic. the genetic distance matrix was obtained by proportions of shared fr ... | 2004 | 15068334 |
| an economic evaluation of the national schistosomiasis control programme in china from 1992 to 2000. | the world bank loan project, by far the largest effort in china for schistosomiasis control since control activities were initiated in the mid 1950s, was carried out for a 9-year period commencing in 1992 in the 8 provinces where schistosoma japonicum remained endemic when the project started. to evaluate its impact, a retrospective economic evaluation was done in 2001. six representative counties, i.e. huarong in hunan province, qianjiang in hubei province, yugan in jiangxi province, tongling i ... | 2005 | 16154104 |
| [comparative study on the resting habit of anopheles minimus a and anopheles minimus c in yunnan province]. | to compare the difference of resting habitat between anopheles minimus a and an. minimus c in yunnan province. | 2005 | 16300003 |
| origin and phylogeographical structure of chinese cattle. | complete mitochondrial d-loop sequences of 231 samples were used to explore the origin and genetic diversity of chinese cattle. phylogenetical analysis of these sequences revealed both bos taurus and bos indicus mitochondrial types in chinese cattle. four of the previously identified mitochondrial dna lineages (t1-t4) were identified in the bos taurus type, including lineage t1, which was found for the first time in chinese cattle. two lineages (i1 and i2) were identified in the bos indicus type ... | 2006 | 17121603 |
| genetic variation of mitochondrial d-loop region and evolution analysis in some chinese cattle breeds. | the complete mitochondrial d-loop region from 123 individuals in 12 chinese cattle breeds and two individuals in germany yellow cattle breed was sequenced and analyzed. the results were shown as follows: 93 variations and 57 haplotypes were detected, and the average number of nucleotide difference was 22.708, nucleotide diversity (d) was 0.0251 +/- 0.00479, and haplotype diversity (hd) was 0.888 +/- 0.026, indicating very high genetic diversity in chinese cattle breeds. in the neighbor-joining t ... | 2007 | 17601610 |
| phylogenetic analysis of bartonella detected in rodent fleas in yunnan, china. | previous studies have demonstrated a diversity of bartonella spp. in rodent populations in yunnan province, china. although bartonella spp. have been isolated from cat fleas and cattle ticks collected from their animal hosts, little is known about bartonella carried by rodent fleas. in this study, bartonella dna was detected by polymerase chain reaction (pcr) in two of five species of rodent fleas. these included xenopsylla cheopis and ctenophthalmus lushuiensis, which were collected from rattus ... | 2007 | 17984255 |
| genetic diversity and origin of gayal and cattle in yunnan revealed by mtdna control region and sry gene sequence variation. | there are hump, humpless cattle and gayal distributed in yunnan province, south-west china, but their genetic background remains unclear. to determine the origin and genetic diversity of yunnan gayal and cattle (diqing, nujiang and wenshan cattle), we analysed mtdna control region sequences of 71 samples and sry gene sequences of 39 samples, together with the available sequences in genbank. the neighbour-joining phylogeny and the reduced median network analysis showed that yunnan gayal originate ... | 2010 | 20433524 |
| dominant bacterial communities in the rumen of gayals (bos frontalis), yaks (bos grunniens) and yunnan yellow cattle (bos taurs) revealed by denaturing gradient gel electrophoresis. | the dominant rumen bacteria in gayals, yaks and yunnan yellow cattle were investigated using pcr-dgge approach. the analysis of dgge profiles, identification of dominant bands and phylogenetic analysis 16s rdna sequences in dgge profiles were combined to reveal the dominant bacterial communities and compared the differences between those cattle species. dgge profiles revealed that gayals had the most abundant dominant bacteria and the lowest similarity of intraspecies between individuals than ot ... | 2010 | 21140219 |
| predictors for abundance of host flea and floor flea in households of villages with endemic commensal rodent plague, yunnan province, china. | from 1990 to 2006, fifty-five natural villages experienced at least one plague epidemic in lianghe county, yunnan province, china. this study is aimed to document flea abundance and identify predictors in households of villages with endemic commensal rodent plague in lianghe county. | 2011 | 21468306 |
| molecular diversity of bacteria in yunnan yellow cattle (bos taurs) from nujiang region, china. | the rumen content of four yunnan yellow cattle (bos taurs) were collected to determine the bacteria diversity by using 16s rrna gene sequence analysis. a total of 129 sequences were examined and the sequences were referred as 107 otu (operational taxonomy unit) according to the similarity level of 97% in gene sequence. similarity analysis revealed that yunnan yellow cattle had 12 sequences (10 otu) shared 97% or greater similarity with cultured rumen bacteria butyrivibrio fibrisolvens, succinicl ... | 2011 | 21598111 |
| association of scd1 and dgat1 snps with the intramuscular fat traits in chinese simmental cattle and their distribution in eight chinese cattle breeds. | intramuscular fat (imf) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl coa desaturase (scd1) and diacylglycerol-acyl transferase 1 (dgat1) genes in cattle. the object of this research was to evaluate the effect of scd1 and dgat1 polymorphisms on imf trait in beef cattle and to estimate the frequency distribution of snps in the two genes in chinese cattle populations. the scd1 and dgat1 polymorphisms were detected by pcr-singl ... | 2011 | 21607624 |
| spotted fever group rickettsia in yunnan province, china. | abstract information about spotted fever group (sfg) rickettsiae in southern china remains sparse. a specific and sensitive real-time pcr assay for detection of sfg rickettsiae was established and used to detect the prevalence rate of sfg rickettsiae in yunnan province, china. the limit of detection (lod) of our real-time pcr was 200 copies per reaction, which is more sensitive than the previously developed nested pcr assays for rickettsia. we tested 265 blood samples (127 goats, 78 dogs, and ... | 2012 | 22225423 |
| [effects of planting rubber and crops on the keeping number of livestock and poultry in the naban river watershed national nature reserve]. | a total of 207 householders from 13 natural villages in the naban river watershed national nature reserve were chosen for a systematic survey on the farming and livestock husbandry, aimed to evaluate the effects of planting rubber and crops on the keeping number of livestock and poultry in the study area. the livestock and poultry bred in the reserve were mainly buffalo, yunnan yellow cattle, small-eared pig, chahua chicken, and a few banna game fowls. exotic breeds were very limited. from 1991 ... | 2011 | 22263476 |