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detection of ehrlichia spp. in raccoons (procyon lotor) from georgia.raccoons (procyonis lotor) and opossums (didelphis virginianus) acquired from six contiguous counties in the piedmont physiographic region of georgia were investigated for their potential role in the epidemiology of ehrlichial and anaplasmal species. serum was tested by indirect fluorescent antibody (ifa) assay for the presence of antibodies reactive to ehrlichia chaffeensis, e. canis, and anaplasma phagocytophilum (hga agent). nested polymerase chain reaction (pcr) assay was used to test whole ...200516011433
prevalence of the agent of human granulocytic ehrlichiosis in ixodes scapularis (acari: ixodidae) in the coastal southeastern united states.human granulocytic ehrlichiosis (hge) is an emerging tick-borne disease recently recognized in the united states. the blacklegged tick, ixodes scapularis say, is the principle vector in the eastern united states. the disease has been commonly reported in the northeastern and upper midwestern states; however, suitable vectors and reservoir hosts exist in the southeast. to assay the prevalence of the hge agent in vector ticks, we screened 818 individual i. scapularis from 15 locations in south car ...200211931023
ehrlichia chaffeensis in archived tissues of a white-tailed deer.white-tailed deer (odocoileus virginianus) play an integral role in the natural history of ehrlichia chaffeensis, the causative agent of human monocytic ehrlichiosis (hme). paraffinized tissues from a white-tailed deer submitted as a diagnostic case to the southeastern cooperative wildlife disease study (athens, georgia, usa) in october of 198.5 and originally described as infected with an unidentified rickettsial organisim were re-examined by specific nested polymerase chain reaction (pcr) for ...199910479099
antibodies to granulocytic ehrlichiae in white-footed and cotton mice in eastern united states.serum samples, collected from peromyscus leucopus (white-footed mouse) or peromyscus gossypinus (cotton mouse) during 1987 through 1990 in florida, georgia, maryland, mississippi, and north carolina (usa), and in 1997 in southern connecticut were analyzed by indirect fluorescent antibody (ifa) staining methods or western blot procedures for antibodies to granulocytic ehrlichiae. of the 82 sera from white-footed mice in connecticut tested by ifa methods with either the bds or nch-1 strain of the ...199910231752
ehrlichia spp. in cervids from california.blood samples from six mule deer (odocoileus hemionus hemionus), 15 black-tailed deer (o. hemionus columbianus), and 29 elk (cervus elaphus nannodes) were assayed for human monocytic and human granulocytic ehrlichiosis (hge) by polymerase chain reaction (pcr), dna sequencing, and serology to determine whether or not cervids are involved in the maintenance of these potential human pathogens in california (usa). the deer were sampled in august to october 1992-95. the 29 tule elk from point reyes n ...19989813842
serologic evidence of infection with ehrlichia spp. in wild rodents (muridae: sigmodontinae) in the united states.rodent (muridae: sigmodontinae) blood and sera collected from 14 states were tested for seroreactivity to a cultured isolate of the human granulocytic ehrlichiosis (hge) agent by using an indirect immunofluorescence assay. of the 1,240 samples tested, 136 (11%) were found to be reactive at titers of > or = 32. rodents with hge agent-specific antibodies were found in new york (23% of 491 samples; geometric mean endpoint titer [gmt] = 441), connecticut (11% of 100 samples; gmt = 481), california ( ...19989508298
detection of anaplasma phagocytophilum in ixodid ticks from equine-inhabited sites in the southeastern united states.abstract anaplasma phagocytophilum is a vector-borne, obligate intracellular bacterium that invades the neutrophils and eosinophils of infected individuals, causing granulocytic anaplasmosis. equine cases have previously been reported in the united states from california, florida, and connecticut, but limited surveillance studies in the southeast have been conducted. the objective of this study was to determine a. phagocytophilum prevalence in ixodes scapularis ticks at southeastern u.s. horse ...201222214269
experimental and field studies on the suitability of raccoons (procyon lotor) as hosts for tick-borne pathogens.we investigated the experimental susceptibility and natural exposure of raccoons (procyon lotor) to five tick-borne pathogens of human and veterinary importance, ehrlichia canis, e. chaffeensis, e. ewingii, anaplasma phagocytophilum (apvariant 1 and ap-ha hge-1 strains), and borrelia lonestari. infections were assessed by polymerase chain reaction (pcr), indirect fluorescent antibody (ifa) testing, and/or culture isolation methods for at least 30 days postinoculation (dpi). two e. chaffeensis-in ...200818429696
detection of babesia and anaplasma species in rabbits from texas and georgia, usa.rabbits have been shown to harbor a suite of zoonotic organisms, including a babesia species, borrelia burgdorferi, and anaplasma phagocytophilum. in this study, we conducted a molecular survey for various tick-borne pathogens in three species of rabbits from texas and georgia. of 18 black-tailed jackrabbits (lepus californicus) tested from texas, six (28%) were polymerase chain reaction (pcr) positive for babesia, and nucleotide sequencing revealed two distinct species or strains. two jackrabbi ...200616584322
the pcs20 pcr assay for ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the united states of america.white-tailed deer are susceptible to heartwater (ehrlichia [cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the united states. it is vital, therefore, to validate a highly specific and sensitive detection method for e. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as ehrlichia chaffeensis, anaplasma (ehrlichia) phagocytophilum (hge agent) and ...200415373331
molecular and serologic evidence of tick-borne ehrlichiae in three species of lemurs from st. catherines island, georgia, usa.in recent years, several species of ehrlichiae have been recognized as tick-borne disease agents of veterinary and medical importance. clinically normal free-ranging or previously free-ranging lemurs, including 46 ring-tailed lemurs (lemur catta), six blue-eyed black lemurs (eulemur macaco flavifrons), and four black and white ruffed lemurs (varecia variegata variegata) from st. catherines island, georgia, were tested for evidence of exposure to tick-borne ehrlichiae. all 52 adult lemurs were se ...200415732591
ticks and tick-borne pathogens and putative symbionts of black bears (ursus americanus floridanus) from georgia and florida.ticks were collected from 38 black bears (ursus americanus floridanus) from northwestern florida (n = 18) from 2003 to 2005 and southern georgia (n = 20) in 2006. five species (amblyomma americanum, a. maculatum, dermacentor variabilis, ixodes scapularis, and i. affinis) were collected from florida bears, and 4 species (a. americanum, a. maculatum, d. variabilis, i. scapularis) were collected from bears in georgia. ixodes scapularis was the most frequently collected tick, followed by d. variabil ...200919413369
reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.to test serum samples of dogs and horses by use of class-specific recombinant-based elisa for establishing a diagnosis of granulocytic ehrlichiosis attributable to infection with organisms from the ehrlichia phagocytophila genogroup.200111560261
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