detection of babesia and anaplasma species in rabbits from texas and georgia, usa.rabbits have been shown to harbor a suite of zoonotic organisms, including a babesia species, borrelia burgdorferi, and anaplasma phagocytophilum. in this study, we conducted a molecular survey for various tick-borne pathogens in three species of rabbits from texas and georgia. of 18 black-tailed jackrabbits (lepus californicus) tested from texas, six (28%) were polymerase chain reaction (pcr) positive for babesia, and nucleotide sequencing revealed two distinct species or strains. two jackrabbi ...200616584322
passive surveillance of ixodes scapularis (say), their biting activity, and associated pathogens in massachusetts.a passive surveillance of tick-borne pathogens was conducted over a 7-year period (2006-2012), in which a total of 3551 ticks were submitted to the university of massachusetts for pcr testing. the vast majority of these ticks were ixodes scapularis from massachusetts (nā€‰=ā€‰2088) and hence were the focus of further analysis. two taqman duplex qpcr assays were developed to test i. scapularis ticks for the presence of three human pathogens: borrelia burgdorferi, anaplasma phagocytophilum, and babesi ...201627248292
detection of human granulocytic ehrlichiosis agent and borrelia burgdorferi in ticks by polymerase chain ixodid ticks were collected from westchester county, new york, and ipswich, massachusetts, to determine the presence of infection with a human granulocytic ehrlichiosis (hge) agent by using the polymerase chain reaction (pcr). the presence of borrelia burgdorferi in ticks collected from new york was also determined by pcr. of the 229 ticks from new york and 47 ticks from massachusetts, 9% (22/229) and 25% (12/47) of ticks contained hge agent, respectively. fifty-four percent (123/229) of t ...19989526861
enzootic transmission of anaplasma bovis in nantucket cottontail rabbits.serological studies of cottontail rabbits sampled from nantucket island, mass., have suggested exposure to at least two ehrlichiae. the agent of human granulocytic ehrlichiosis (anaplasma phagocytophilum) is intensely enzootic in rabbits there, but the identity of the other ehrlichial infection remains undescribed. we sampled rabbits over five transmission seasons and tested their blood and tissues for evidence of infection using pcr targeting an ehrlichia genus-wide 16s rdna target. sequence an ...200312904385
hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of dna of agent of human granulocytic ehrlichiosis by pcr.ixodid ticks were collected from connecticut, massachusetts, missouri, pennsylvania, rhode island, and british columbia (canada) during 1991 to 1994 to determine the prevalence of infection with hemocytic (blood cell), rickettsia-like organisms. hemolymph obtained from these ticks was analyzed by direct and indirect fluorescent antibody (fa) staining methods with dog, horse, or human sera containing antibodies to ehrlichia canis, ehrlichia equi, or rickettsia rickettsii. of the 693 nymphal and a ...19958567911
clinical findings and diagnosis in human granulocytic anaplasmosis: a case series from describe clinical findings and the use of a tick-associated pathogen panel in a series of patients with human granulocytic anaplasmosis (hga) at a suburban boston hospital.201222386178
reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays.sera from 35 patients diagnosed with human granulocytic ehrlichiosis in connecticut were tested by indirect ifa staining methods with 5 strains of ehrlichia equi or the human granulocytic ehrlichiosis agent to assess the suitability of different strains in laboratory analyses. antigens included horse-derived infected neutrophils (mrk and bds strains) and human isolates cultured in human promyelocytic leukemia cells (nch-1, rch, and webster). of 35 sera, 23 (65.7%) reacted to all 5 strains. serop ...19989815246
enzootic transmission of babesia divergens among cottontail rabbits on nantucket island, massachusetts.specific ticks seem to locally serve as vector for characteristic microbial assemblages (guilds) comprising spirochetes, piroplasms, ehrlichiae, and arboviruses. borrelia andersoni and anaplasma phagocytophilum are intensely transmitted between cottontail rabbits. to test the hypothesis that a piroplasm may also be maintained in rabbits, we sampled these hosts from nantucket island, massachusetts and tested their blood and tissues by a polymerase chain reaction for evidence of infection. surpris ...200314695079
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