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genetic heterogeneity of borrelia burgdorferi sensu lato in the southern united states based on restriction fragment length polymorphism and sequence analysis.fifty-six strains of borrelia burgdorferi sensu lato, isolated from ticks and vertebrate animals in missouri, south carolina, georgia, florida, and texas, were identified and characterized by pcr-restriction fragment length polymorphism (rflp) analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons. a total of 241 to 258 bp of intergenic spacers between tandemly duplicated rrf (5s) and rrl (23s) was amplified by pcr. msei and drai restriction fragment polymorphisms were used to analyze these ...200111427560
antibodies to borrelia burgdorferi in rodents in the eastern and southern united states.serologic studies were conducted to determine whether white-footed mice (peromyscus leucopus) and cotton mice (peromyscus gossypinus) contained serum antibodies to borrelia burgdorferi, the causative agent of lyme borreliosis. enzyme-linked immunosorbent assays detected antibodies to this spirochete in 35.7 and 27.3% of 56 p. leucopus and 535 p. gossypinus serum samples, respectively, collected in connecticut, north carolina, south carolina, georgia, florida, alabama, and mississippi. antibody t ...19921624561
the bascom palmer eye institute lyme/syphilis survey.serologic screening of patients for lyme borreliosis began at the bascom palmer eye institute (bpei) in september 1987. this report reviews the data on 641 sera from that date up to january 1, 1990. initially only immunofluorescent (ifa) igg and igm titers were obtained. because of increasing numbers of borderline and positive ifa tests, a lyme enzyme linked immunosorbent assay (elisa) was added in april 1988. also, because of significant serologic cross reactivity in patients exposed to trepone ...19902150843
rodent ectoparasites from two locations in northwestern florida.from feb.-apr. 1999, 19 species of ectoparasitic arthropods (2 sucking lice, 4 fleas, 4 ticks, 2 mesostigmatid mites, 5 chiggers, 2 fur mites) were recovered from 106 rodents belonging to 5 species (cotton mouse, peromyscus gossypinus, n = 64; cotton rat, sigmodon hispidus, n = 23; eastern woodrat, neotoma floridana, n = 9; golden mouse, ochrotomys nuttalli, n = 9; eastern gray squirrel, sciurus carolinensis, n = 1) at tall timbers research station, leon county, florida. during the same period, ...200011217221
antibodies to borrelia burgdorferi in deer and raccoons.an enzyme-linked immunosorbent assay (elisa) was developed to detect serum antibodies to borrelia burgdorferi, the causative agent of lyme borreliosis, in deer (odocoileus virginianus) and raccoons (procyon lotor). blood samples were collected from these mammals in connecticut, maryland, north carolina, georgia and florida. seropositivity for deer was highest in connecticut (56% of 353 sera) and maryland (51% of 35 sera). raccoons in connecticut, maryland, north carolina, and florida also had an ...19911758022
comparison of the spirochete borrelia burgdorferi s. l. isolated from the tick ixodes scapularis in southeastern and northeastern united states.thirty-five strains of the lyme disease spirochete borrelia burgdorferi sensu lato (b. burgdorferi s. l.) were isolated from the blacklegged tick vector ixodes scapularis in south carolina, georgia, florida, and rhode island. they were characterized by pcr-restriction fragment length polymorphism (rflp) analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons. pcr-rflp analysis indicated that the strains represented at least 3 genospecies (including a possible novel genospecies) and 4 differen ...200818576863
prevalence of tick-borne pathogens in host-seeking amblyomma americanum (acari: ixodidae) and odocoileus virginianus (artiodactyla: cervidae) in florida.amblyomma americanum (l.), the lone star tick, is an aggressive tick that is expanding its geographic range within the united states. this tick is the vector for the human and veterinary pathogens ehrlichia chaffeensis and ehrlichia ewingii and is associated with other microbes of unspecified pathogenicity including rickettsia amblyommii, panola mountain ehrlichia, and borrelia lonestari in florida, there has been sparse contemporary data on the prevalence of these organisms in host-seeking lone ...201627117680
different populations of blacklegged tick nymphs exhibit differences in questing behavior that have implications for human lyme disease risk.animal behavior can have profound effects on pathogen transmission and disease incidence. we studied the questing (= host-seeking) behavior of blacklegged tick (ixodes scapularis) nymphs, which are the primary vectors of lyme disease in the eastern united states. lyme disease is common in northern but not in southern regions, and prior ecological studies have found that standard methods used to collect host-seeking nymphs in northern regions are unsuccessful in the south. this led us to hypothes ...201525996603
borrelia burgdorferi dna absent, multiple rickettsia spp. dna present in ticks collected from a teaching forest in north central florida.tick-borne diseases are an emerging public health threat in the united states. in florida, there has been public attention directed towards the possibility of locally acquired borrelia burgdorferi sensu stricto, the causative agent of lyme disease, in association with the lone star tick. the aim of this study was to determine the prevalence of ticks and the pathogens they carry and potentially transmit, such as b. burgdorferi, in a highly utilized teaching and research forest in north central fl ...201727720381
prevalence of dirofilaria immitis, ehrlichia canis, and borrelia burgdorferi in pet dogs, racing greyhounds, and shelter dogs in florida.arthropod vectors of canine infectious diseases are present throughout florida. since crowded housing has the potential to bring vectors and infected dogs into close proximity, it is possible that prevalence of infection is higher in intensely housed dogs. in this study, the seroprevalence of dirofilaria immitis, ehrlichia canis, and borrelia burgdorferi in dogs residing in two types of intensive housing, greyhound kennels and animal shelters, was compared to dogs residing in low-intensity housi ...201020399018
nuclear markers reveal predominantly north to south gene flow in ixodes scapularis, the tick vector of the lyme disease spirochete.ixodes scapularis, the tick vector of the lyme disease spirochete, is distributed over most of the eastern united states, but >80% of all lyme disease cases occur in the northeast. the role that genetic differences between northern and southern tick populations play in explaining this disparate distribution of lyme disease cases is unclear. the present study was conducted with 1,155 snp markers in eight nuclear genes; the 16s mitochondrial gene was examined for comparison with earlier studies. w ...201526536360
absence of borrelia burgdorferi in patients with localized scleroderma (morphea). 19957673508
lyme borreliosis in human patients in florida and georgia, usa.the aim of this study was to determine the cause of illness in several human patients residing in florida and georgia, usa, with suspected lyme disease based upon em-like skin lesions and/or symptoms consistent with early localized or late disseminated lyme borreliosis. using polymerase chain reaction (pcr) assays developed specifically for lyme group borrelia spp., followed by dna sequencing for confirmation, we identified borrelia burgdorferi sensu lato dna in samples of blood and skin and als ...201323781138
natural infections with borrelia spirochetes in two dogs from florida.spirochetemia is a rarely reported observation in dogs. we describe the clinical, hematologic, and immunodiagnostic features of two spirochetemic dogs from northern florida and the subsequent isolation and preliminary characterization of a borrelia species from one dog in which culture of a sample for spirochetes was attempted. results of polyacrylamide gel electrophoresis, monoclonal antibody testing, and pcr analysis indicate that the florida isolate is not borrelia burgdorferi, the only other ...19948150943
borrelia species in host-seeking ticks and small mammals in northern florida.the aim of this study was to improve understanding of several factors related to the ecology and environmental risk of borrelia infection in northern florida. small mammals and host-seeking adult ticks were collected at several sites, and specimens were tested for the presence of borrelia species, primarily by pcr amplification. tissues from some vertebrates and ticks were initially cultured in bsk-h medium to isolate spirochetes, but none were recovered. however, comparison of partial flagellin ...200415528699
the prevalence of lyme disease in a nonendemic area. a comparative serologic study in a south florida eye clinic population.serologic tests for lyme borreliosis and for syphilis were performed on 75 patients seen in a 1-week period at the bascom palmer eye institute in miami. the incident of syphilis was 8% and of lyme borreliosis 3% in this study in a nonendemic area. the most common cause for a high titer serologic response for lyme borreliosis in this group was a prior treponema pallidum infection. the importance of getting vdrl, fta-abs, lyme ifa, and lyme elisa tests in all suspected cases was emphasized.19892529271
evaluation of ixodes scapularis, amblyomma americanum, and dermacentor variabilis (acari: ixodidae) from georgia as vectors of a florida strain of the lyme disease spirochete, borrelia burgdorferi.the ability of three common tick species from georgia to maintain and transmit the causative agent of lyme disease, borrelia burgdorferi, was compared under laboratory conditions. a b. burgdorferi cotton rat isolate (mi-6) from florida was selected as a strain from the south, and the sh2-82 isolate from new york was used as a positive control. amblyomma americanum (l.) and dermacentor variabilis (say) did not transmit the mi-6 isolate from inoculated hamsters to naive laboratory mice, and nympha ...19957650697
natural occurrence and characterization of the lyme disease spirochete, borrelia burgdorferi, in cotton rats (sigmodon hispidus) from georgia and florida.this is the first report of natural infection by borrelia burgdorferi in the cotton rat sigmodon hispidus. nine b. burgdorferi isolates were obtained from ear tissues, urinary bladders, or both, by culturing tissues in bskii medium. the rat from which the si-3 isolate was cultured was from the same site (sapelo island, georgia) as an infected cotton mouse peromyscus gossypinus and ixodes scapularis tick reported previously. the 8 b. burgdorferi isolates from rats in florida included 1 (ai-1) fro ...19957876974
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