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genospecies identification and characterization of lyme disease spirochetes of genospecies borrelia burgdorferi sensu lato isolated from rodents in taiwan.lyme disease spirochetes of the genospecies borrelia burgdorferi sensu lato were identified and characterized for the first time in taiwan. seven isolates, designated twkm1 to twkm7, were purified from the ear tissues of three species of rodents captured from seven localities of taiwan. the immunological characteristics of these taiwan isolates were compared with those of other genospecies of lyme disease spirochetes by analyzing the protein profiles and reactivities with b. burgdorferi-specific ...19989774551
negative incidence of lyme disease-related borrelia spp. in alishan, taiwan.to investigate the prevalence of lyme disease-related borrelia species, wild rodents were captured around yushan national park and alishan forest recreation area park in taiwan 2,000 to 3,000 meters above sea level. borrelia was not isolated from 67 small mammals of 7 species. sera from rodents showed no positive reactivity against whole cell antigens of b. garinii, b. afzelii or b. valaisiana by elisa. these results suggested that lyme disease is not endemic to the alishan area.200111471827
an ospa-based genospecies identification of lyme disease spirochetes (borrelia burgdorferi) isolated in taiwan.to further identify the genospecies of lyme disease spirochetes (borrelia burgdorferi) isolated in taiwan, we analyzed the genomic identities of these taiwan isolates (twkm1-7) by genospecies-specific polymerase chain reaction (pcr) assay, restriction fragment length polymorphism (rflp) analysis, and gene sequencing based on the ospa gene sequences of b. burgdorferi sensu lato. pcr analysis indicates that all of these taiwan isolates were genetically related to the genospecies of b. burgdorferi ...200212201600
molecular analysis of ixodes granulatus, a possible vector tick for borrelia burgdorferi sensu lato in taiwan.the genetic identity of ixodes granulatus ticks was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 19 strains of ticks representing seven species of ixodes and two outgroup species (rhipicephalus sanguineus and haemaphysalis inermis). four major clades could be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granul ...200919184580
molecular detection and genetic identification of borrelia garinii and borrelia afzelii from patients presenting with a rare skin manifestation of prurigo pigmentosa in taiwan.to determine the genetic identity of borrelia spirochetes isolated from patients with an unusual skin lesion of prurigo pigmentosa (pp) in taiwan. the causative agents responsible for human borreliosis were clarified.201324103333
first isolation and molecular identification of borrelia burgdorferi sensu stricto and borrelia afzelii from skin biopsies of patients in taiwan.in order to clarify the transmission cycle and genetic identity of borrelia spirochetes in the non-endemic country of taiwan, the causative agents responsible for human borreliosis were isolated from skin biopsies of patients and their genetic identities were determined.201021147012
first detection and molecular identification of borrelia garinii isolated from human skin in taiwan. 201019910482
genetic analysis of the outer surface protein c gene of lyme disease spirochaetes (borrelia burgdorferi sensu lato) isolated from rodents in taiwan.the outer surface protein c gene (ospc) of lyme disease spirochaetes (borrelia burgdorferi sensu lato) was analysed for the first time in taiwan. the genetic identities of these taiwan isolates (twkm1-7) were determined by restriction fragment length polymorphism (rflp) analysis and sequence similarities of the pcr-amplified ospc gene amplicons. after cleavage by nuclease dral, differential fragment patterns of pcr-amplified ospc dna in relation to different genospecies of lyme disease spirochae ...200211926737
the presence of borrelia valaisiana-related genospecies in ticks and a rodent in taiwan.a field survey was conducted to investigate the presence of borrelia burgdorferi sensu lato (s.l.) in six counties of taiwan. spirochetes were successfully isolated from one rodent ear sample out of 485 rodent ears and 53 live, fed tick (ixodes granulatus) samples. the spirochetes were confirmed to be b. burgdorferi s.l. by real-time pcr. in addition, 23 of 113 tick samples were tested positive for borrelia dna according to real-time pcr. the borrelia isolate from the rodent and the 23 borrelia ...201021221950
acute neuroborreliosis with involvement of the central nervous system.lyme neuroborreliosis has not been previously reported in taiwan. the purpose of this study was to describe the important characteristics of lyme neuroborreliosis caused by borrelia burgdorferi-like species in taiwanese patients.201020561635
first detection and molecular identification of borrelia burgdorferi-like spirochetes in ixodes granulatus ticks collected on kinmen island of taiwan.borrelia burgdorferi-like spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic analysis reveals that the sequence similarity of these spirochetes, ranging from 90.5% to 98.7% is highly homogeneous wi ...200919270287
lyme disease in taiwan: first human patient with characteristic erythema chronicum migrans skin lesion.we report herein the first laboratory-diagnosed case of lyme disease in a human in taiwan. a 45-year-old taiwanese man living in taipei, in northern taiwan, had an expanding skin lesion (measuring 23 by 15 cm) on his abdomen for 2 to 3 weeks and recurrent attacks of pain and swelling of the knee joint. serologic tests indicated a significantly elevated titer of antibody to borrelia burgdorferi. after appropriate antibiotic treatment for 3 weeks, the skin lesion was cured and the joint swelling w ...19989508317
molecular characterization of lyme disease spirochetes (borrelia burgdorferi sensu lato) isolated in taiwan by restriction fragment length polymorphism analysis of 5s(rrf)-23s(rrl) intergenic spacer amplicons.we analyzed the 5s (rrf)-23s (rrl) intergenic spacer amplicon gene of lyme disease spirochetes (borrelia burgdorferi sensu lato) for the first time in taiwan. the genetic identities of these taiwan isolates (twkm1-7) were clarified by comparing their restriction fragment length polymorphism patterns and sequence similarities of the polymerase chain reaction-amplified intergenic spacer amplicon genes with 3 major genospecies of lyme disease spirochetes. amplified-spacer dnas were purified further ...200212479552
lyme disease in childhood: report of one case.we report a pediatric patient of lyme disease in taiwan, confirmed by laboratory tests. an 8-year-old overseas chinese girl from new jersey, usa, visited our dermatological clinic with joint pain and multiple expanding annular erythema rashes (measured up to 17x10 cm) for three days. lyme disease was diagnosed by the presence of the antibody against borrelia burgdorferi. the skin lesions and arthralgia were resolved after amoxicillin treatment, and she got well in the following one year. this pr ...200212148968
lyme disease in taiwan: primary isolation of borrelia burgdorferi-like spirochetes from rodents in the taiwan area.to investigate the prevalence of lyme disease infection in taiwan, we conducted a zoonotic survey for spirochetal infection in the small mammals. ear tissues of trapped rodents collected from various localities in taiwan were incubated into bsk-h culture medium and examined for the evidence of spirochetal infection by dark-field microscopy. spirochetes cultured from six species of wild and peridomestic rodents and seven isolates, designated twkm 1-7, were purified by serial dilution and membrane ...19989840582
a preliminary report on borrelia burgdorferi infection in the taiwan area.a total of 273 serum specimens from different areas and sources were tested against borrelia burgdorferi antigens by enzyme-linked immunosorbent assay method. positive rates of serological reactions were 3% and 58% for healthy persons and syphilis patients, respectively. obviously, there was a lot of cross-reaction in the venereal disease group. meanwhile, positive rates were 3% and 13% in the sera collected from taiwan and orchid island, respectively. this difference may reflect a less develope ...19949747351
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