| seroepidemiological survey for antibody to borrelia burgdorferi in cows. | antibody to borrelia burgdorferi was examined in 380 healthy and 38 clinical cases of cows from hokkaido and shizuoka in japan. in healthy animals, igg and igm antibody to b. burgdorferi ho14 strain were found in 44 cows (14.6%) and 24 cows (8.0%) from hokkaido. in contrast, antibody-positive case was not observed except for only 1 case which was igm positive (1/79: 1.3%) in cows from shizuoka. mean antibody levels of healthy animals in hokkaido and shizuoka were 0.651 and 0.263 (igg antibody to ... | 1992 | 1479959 |
| current situation of bovine virus diarrhoea-mucosal disease (bvd-md) virus infections and their antigenic diversity in hokkaido, japan. | the current situation of bovine virus diarrhoea-mucosal disease (bvd-md) virus infections is briefly reviewed, with special reference to problems arising from fetal infection in the hokkaido district, a northern island of japan. the results of some investigations on bvd-md virus carried out in japan are also described. although bovine congenital anomalies caused by bvd-md virus and mucosal disease may be late sequelae to fetal infection and occur sporadically, it appears that their incidence and ... | 1990 | 1966723 |
| epidemiology of abomasal nematodes of dairy cattle in hokkaido, northern japan. | the prevalence and intensity of infection with abomasal nematodiasis was studied in dairy cattle of hokkaido, northern japan, for successive two years. during the period of march in 1985 to september in 1987, a total number of 393 abomasa of holstein-friesian cows was examined for nematode parasites. nematodes were detected from 75% of the cows. the prevalence of nematode species detected was ostertagia ostertagi 250 (63.6%), mecistocirrus digitatus 181 (46.1%), trichostrongylus axei 85 (21.6%) ... | 1990 | 2348599 |
| a survey of abomasal and duodenal nematodes in cattle in hokkaido, japan. | | 1987 | 3626236 |
| survey for antibodies to chlamydia psittaci in cattle in hokkaido. | | 1987 | 3682527 |
| a serological survey of infectious bovine rhinotracheitis in cattle in hokkaido, japan. | | 1973 | 4736972 |
| [statistical considerations on the bulls used at the artificial insemination centers in hokkaido from 1958-65]. | | 1967 | 5299530 |
| leptospira interrogans serovar hardjo infection in cows of hokkaido, japan--serological evidence. | | 1983 | 6200638 |
| incidence of lymphocytic nuclear pockets in bovine leukosis observed in hokkaido. | | 1983 | 6632476 |
| a study on heifer mastitis in japan and its causative microorganisms. | survey and bacteriological examination were conducted on heifer mastitis in hokkaido, the northern island of japan over a period from 1976 to 1981. the disease occurred in heifers, mostly 13 to 18 months old, less than a half of which was pregnant in late summer or in august and september. infection occurred predominantly in a front quarter than a rear one. some microorganisms were isolated by the co2 gas jet method from all the 74 secretions from the affected quarters of 67 heifers. actinomyces ... | 1983 | 6677834 |
| studies on mycobacterial infection of cows in hokkaido. | | 1981 | 7289324 |
| statistical considerations concerning the disposal of dairy bulls used for artificial insemination in hokkaido from 1958 to 1979. | | 1981 | 7343687 |
| survey of sarcocystis infection in cattle in east hokkaido, japan. | the prevalence of sarcocystis infection was examined in older cows and imported cattle slaughtered in east hokkaido. samples of myocardial tissues were examined for sarcocystis microscopically. sarcocystis cysts were detected in 15.7% of 83 older cows, 48.4% of 91 imported cattle which were kept in east hokkaido prior to slaughter and 51.1% of 94 imported cattle slaughtered immediately after quarantine check. based on the morphology of the cyst wall and the establishment of infection in experime ... | 1994 | 7948391 |
| rumen ciliate composition and diversity of japanese beef black cattle in comparison with those of holstein-friesian cattle. | the composition of ciliate protozoa in the rumen of 30 japanese beef black cattle (bos taurus taurus) fed in yoron island, kagoshima, was compared with that of 125 holstein-friesian cattle (bos taurus taurus) fed in hokkaido. thirteen genera including 44 species with 32 formae detected from japanese beef black cattle and 15 genera including 48 species with 25 formae from holstein-friesian cattle were identified. thirty-six species with 19 formae were common to both hosts. a new species was recog ... | 1994 | 7999896 |
| serological survey for borrelia burgdorferi infection in cattle in southern hokkaido. | by means of the enzyme-linked immunosorbent assay (elisa), antibodies to borrelia burgdorferi were examined in 970 cattle from southern hokkaido, japan. the seropositivity rate in the cattle less than 2 years old differed significantly from that in those 3 years old or more. a seasonal variation was also observed in the rate of seropositivity to b. burgdorferi. high levels of anti-b. burgdorferi antibody were detected even in sera from a healthy group of cattle, indicating that there are likely ... | 1993 | 8117816 |
| heritability and repeatability for milk production traits of japanese holsteins from an animal model. | records of the first three lactations of cows were obtained from the file of the dairy herd testing association in hokkaido. the data were divided into 10 files containing approximately 24,000 cows each. the derivative-free reml method was used to estimate variances with a repeatability model. the model contained herd-year, calving month, and the linear and quadratic effects of age at calving as fixed effects; additive genetic, permanent environmental, and temporary environmental effects were th ... | 1994 | 8182183 |
| rumen ciliates of ezo deer (cervus nippon yesoensis) with the morphological comparison with those of cattle. | the composition of rumen ciliate protozoa was surveyed in 13 ezo deer, cervus nippon yesoensis, inhabiting hokkaido, japan. as a result, two species of the genus entodinium, e. simplex and e. dubardi, were detected. each ezo deer had only a single species of protozoa, e. simplex or e. dubardi. since e. simplex in ezo deer had a wide variation in body size and shape, its measurements were compared with those of e. simplex, e. nanellum and e. exiguum obtained from holstein-friesian cattle feeding ... | 1993 | 8461433 |
| [a comparative study of public knowledge and awareness of echinococcus in relation to anti-echinococcus policies of towns in hokkaido]. | a population-based comparative study was performed on public awareness and knowledge about the risk of echinococcosis and the life habits of the people living in 2 towns in eastern hokkaido, bekkai and akan. bekkai has had an anti-echinococcosis policy and akan has only limited experiences with such countermeasures. the results of the survey were as follows: 1) compared with the inhabitants in the town of akan, the residents in bekkai had significantly higher correct response rates for many ques ... | 1995 | 8520051 |
| detection of sheep-associated malignant catarrhal fever virus antibodies by complement fixation tests. | some serological diagnosis methods and examinations for detection of antibodies to sheep-associated malignant catarrhal fever (mcf) infection were investigated. the wildebeest-associated mcf virus strain wc11 propagated on fetal bovine thyroid cell cultures was used as an antigen. antibodies were detected by complement fixation (cf) tests in cattle pathologically diagnosed as having sheep-associated mcf, as well as in cattle experimentally infected with mcf virus strain wc11. however, immunodiff ... | 1996 | 8645750 |
| detection of antibodies against bovine immunodeficiency-like virus in daily cattle in hokkaido. | serological survey of bovine immunodeficiency-like virus (biv) infection was performed in cattle of 3 different farms in hokkaido, where a relatively high seroprevalence was recorded for bovine leukemia virus (blv). about a half of 120 cattle tested were seropositive for blv, while 7.5% of the cattle were seropositive for biv. though increased numbers of leukocytes were frequently observed in blv-seropositive cows, no such changes were observed in biv-positive but blv-negative cows. no correlati ... | 1996 | 8741608 |
| a polymorphism observed in the experimentally successful peptide vaccine sequence derived from theileria sergenti piroplasm major surface antigen (p33). | a polymorphism in the experimentally successful peptide vaccine sequence (evvwkekkevkdlda, amino acids 134-148) derived from the 33 kda piroplasm major surface antigen (p33) of theileria sergenti was examined. the vaccine sequences obtained by pcr amplification and sequencing of the p33 gene from a total of 15 parasite-infected cattle blood samples collected from 4 prefectures through hokkaido to kumamoto revealed the two major sequences (ikeda and chitose stock types) either of which was identi ... | 1997 | 9342711 |
| detection of coxiella burnetii from dust in a barn housing dairy cattle. | we attempted to detect coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (pcr) method. ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near sapporo, hokkaido. c. burnetii was detected in 5 of the 10 dust samples. it was believed that aerial contamination by c. burnetii occurred in the barn. | 1998 | 9525780 |
| epidemiology of tick-borne encephalitis in japan. | a tick-borne encephalitis (tbe) patient has not been reported for many years in japan although a serological survey of domestic animal sera suggested the presence of tbe foci in hokkaido, the northern island of japan. in october 1993 in hokkaido, a severe encephalitis case in a farm wife was diagnosed as tick-borne encephalitis. serological examination of paired sera showed a rise of neutralization (nt) antibody titer to russian spring summer encephalitis virus. a sero-epizootiological survey of ... | 1998 | 9611678 |
| detection of escherichia coli o157:h7 from musca domestica (diptera: muscidae) at a cattle farm in japan. | enterohemorrhagic escherichia coli (ehec) o157:h7 was isolated for the first time from musca domestica l. a total of 310 fly samples was collected from 4 different farms in obihiro-city, hokkaido, in the summer and autumn of 1997;5 samples carried e. coli serotype o157:h7. using elisa and vero cell cytotoxicity assay, 3 isolates from 1 cattle farm produced both active shiga-toxin type 1 (stx1) and 2 (stx2). these isolates also carried hemolysin and eaea genes and harbored the 90-kb virulence pla ... | 1999 | 10071501 |
| first detection of bovine group b rotavirus in japan and sequence of its vp7 gene. | an epizootic outbreak of diarrhea occurred in adult cows on a dairy farm in hokkaido, japan. one colostrum-fed calf inoculated with pooled feces of the 5 affected cows, developed mild diarrhea, and shed rotavirus-like particles which reacted with antiserum to group b rotavirus in immune electron microscopy. cell culture immunofluorescence tests, rna-polyacrylamide gel electrophoresis and rt-pcr confirmed that this virus was bovine group b rotavirus, which was designated the nemuro strain. additi ... | 1999 | 10365171 |
| japanese scrapie cases. | worldwide attention has been given to scrapie, because bovine spongiform encephalopathy (bse) could be experimentally transmitted to sheep. this ovine form of bse was clinically identical to scrapie. in japanese scrapie cases, a majority of the diseased sheep were from suffolk, while 8 cases were from corriedale. it is very likely that sheep-to-sheep transmission of scrapie has taken place in obihiro, hokkaido. normal prion protein may play a role in the morphoregulatory signaling pathway, which ... | 2000 | 10871915 |
| has echinococcus granulosus settled in hokkaido? | | 2000 | 11056566 |
| detection of cryptosporidium parvum oocysts in environmental water in hokkaido, japan. | control of cryptosporidiosis is important in public health. rivers that are polluted with cryptosporidium and drinking water that is treated for drinking water production from polluted rivers could result in the waterborne disease of cryptosporidiosis. we carried out an epidemiological study of natural water supplies in hokkaido, one of the largest dairy prefectures in japan. to detect cryptosporidium oocysts in environmental water, the filtration method was used for 28 samples, which were colle ... | 2001 | 11307921 |
| [quality of raw milk produced in hokkaido and quality control activity on farm]. | | 2001 | 11577398 |
| vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds. | vertical transmission of bovine leukemia virus (blv) and bovine immunodeficiency virus (biv) was investigated in five dairy cattle herds in hokkaido, where 36.1 and 17.0% of cattle were blv and biv seropositive, respectively, and 9.9% of dams were co-infected with both biv and blv. twenty six cases of offspring born from dams infected with only blv (17 cases) or with both biv and blv (9 cases) were examined for the presence of blv and biv before and after colostrum feeding by polymerase chain re ... | 2002 | 11731179 |
| isolation of foot-and-mouth disease virus from japanese black cattle in miyazaki prefecture, japan, 2000. | four outbreaks of foot-and-mouth disease (fmd) occurred from march to may 2000 in miyazaki and hokkaido prefectures, japan. fmd virus isolation was achieved by sampling probang materials from japanese black cattle in the third case found in miyazaki prefecture. the probang materials were inoculated to bovine kidney (bk) and bovine thyroid cell cultures. cpe was observed in the bk at two days post-inoculation. specific amplified dna segments for fmd virus (fmdv) were detected by reverse transcrip ... | 2002 | 11853156 |
| waste management systems of dairy cattle farms in japan. | recently, the size of livestock farms in japan has been expanding and the pollution from farm wastes has become a serious problem in rural areas. therefore it is necessary to design treatment strategies and improve the recycling of livestock manure for sustainability of agriculture in japan. the dairy cattle waste management systems were studied at dairy farms in aomori prefecture and in hokkaido, japan. the four farms, typical for the respective regions in japan, were investigated on the basis ... | 2002 | 12201128 |
| seroprevalence of bovine immunodeficiency virus and bovine leukemia virus in dairy and beef cattle in hokkaido. | serological survey of bovine immunodeficiency virus (biv) and bovine leukemia virus (blv) infection was conducted in dairy cattle from 10 different regions of hokkaido, japan. among 390 cattle, 11.0% of cattle were biv-seropositive and 3.3% were blv-seropositive. moreover, in two dairy farms, where bovine leukosis has been reported, prevalence of biv infections were 6.4 and 9.1%, respectively. in contrast, among 150 beef cattle, 16.6% were biv-seropositive while none was blv-seropositive. dual i ... | 2003 | 12655131 |
| viability and infectivity of cryptosporidium parvum oocysts detected in river water in hokkaido, japan. | the viability and infectivity of cryptosporidium parvum (c. parvum) oocysts, detected in water samples collected from river water in hokkaido, were investigated using severe combined immunodeficient (scid) mice. the water samples collected from september 27 through october 10, 2001 by filtration using cuno cartridge filters were purified and concentrated by the discontinuous centrifugal flotation method. from 1.2 x 10 (5) liters of the raw river water, approximately 2 x 10(4) oocysts were obtain ... | 2003 | 12808210 |
| molecular epidemiological survey of benign theileria parasites of cattle in japan: detection of a new type of major piroplasm surface protein gene. | benign theileria species of cattle are found in most parts of the world. the major piroplasm surface protein (mpsp), a conserved protein in all theileria species, has been used as a maker for epidemiological and phylogenetical studies of benign theileria species. parasites with ikeda- or chitose-type mpsp genes are dominant in japan, but we report here mixed infection cases of theileria parasites with an additional mpsp type parasite infecting cattle in abashiri district, hokkaido. the mpsp gene ... | 2004 | 15107552 |
| evaluating river water quality through land use analysis and n budget approaches in livestock farming areas. | this study was carried out to evaluate the quality of river water by analysis of land use in drainage basins and by estimating the n budgets. the drainage basins of shibetsu river (shibetsu area) and bekkanbeushi river (akkeshi area) in eastern hokkaido, japan were selected for a case study, and the evaluation of water quality was up-scaled to the regional level in hokkaido by using the arcview/gis and statistical information. water sampling was carried out in august 2001 and may 2002 in the shi ... | 2004 | 15262158 |
| susceptibilities against bovine lactoferrin with microorganisms isolated from mastitic milk. | antibacterial effects of bovine lactoferrin were studied in vitro against microorganisms isolated from mastitic milk in tokachi area, hokkaido, japan. microorganisms isolated were escherichia coli (11 isolates), klebsiella pneumoniae (5 isolates), enterococci (8 isolates), staphylococcus aureus (10 isolates), coagulase negative staphylococci (cns, 13 isolates), streptococci (11 isolates), prototheca zopfii (7 isolates) and yeast-like fungi (9 isolates). lactoferrin has been known as a multifunct ... | 2004 | 15528862 |
| parasitic lesions of bovine liver attributed to capillaria species. | among meat inspectors in hokkaido, japan, the term "bovine parasitic hepatitis" (bph) has long been used to refer to a hepatic disorder characterized by multiple small yellowish lesions. however, the aetiology is unknown. by means of detailed histopathological examination, fragments of parasitic worms resembling capillaria were detected in nine (2.25%) of 400 livers showing bph lesions. histologically, the degenerative lesions showed eosinophilic papillary endothelial hyperplasia of the interlob ... | 2005 | 15737350 |
| characteristics and epidemiologic genotyping of staphylococcus aureus isolates from bovine mastitic milk in hokkaido, japan. | two hundred thirty one staphylococcus aureus isolates from bovine mastitic milk were discriminated into 60 patterns and 16 lineages by pulsed-field gel electrophoresis (pfge). the tested isolates were also investigated using coagulase and capsule serotyping and pcr for possession of genes that encode staphylococcal enterotoxins (sea to sei), enterotoxin-like toxins (selj to selr), and toxic shock syndrome toxin (tst). one hundred seventy three of the isolates (74.9%) possessed one or more toxin ... | 2006 | 16520540 |
| development of a survival model with piecewise weibull baselines for the analysis of length of productive life of holstein cows in japan. | a total of 100,000 records of holstein dairy cows in hokkaido, japan, were used to study the current characteristics of their length of productive life, to build up a suitable model for genetic survival analysis, and to apply the piecewise weibull baseline model. the data for this analysis only included records of cows belonging to herds in which more than 60% of cows had type score. compared with other studies, the proportions of cows with only 1 or 2 calvings were low (24 and 46%, respectively ... | 2006 | 16960082 |
| seasonal differences in the parameters of luteinizing hormone release to exogenous gonadotropin releasing hormone in prepubertal holstein heifers in sapporo. | stress due to summer heat has adverse effects on reproduction in holstein dairy cattle. summer suppression of reproduction of holsteins can pose an important economic problem, even in hokkaido prefecture located in the northern region of japan. hokkaido is one of the most important dairy farming areas of japan. this study is an attempt to clarify the seasonal differences in the parameters of luteinizing hormone (lh) response to exogenous gonadotropin releasing hormone (gnrh) in sapporo, hokkaido ... | 2007 | 17008758 |
| assessment of river water quality during snowmelt and base flow periods in two catchment areas with different land use. | river water quality was evaluated with respect to eutrophication and land use during spring snowmelt and summer base flow periods in abashiri (mixed cropland-livestock farming) and okoppe (grassland-based dairy cattle farming), eastern hokkaido, japan. water from rivers and tributaries was sampled during snowmelt and summer base flow periods in 2005, and river flow was measured. total n (tn), no(3)-n, and si concentrations were determined using standard methods. total catchment and upland areas ... | 2008 | 17503203 |
| detection of bovine torovirus in fecal specimens of calves with diarrhea in japan. | the aim of this study was to determine the prevalence of bovine torovirus (botv) in bovine fecal samples and to determine whether a relationship exists between botv and diarrhea in japan. ninety-nine diarrheic and 114 normal fecal samples from calves in hokkaido prefecture and 38 diarrheic fecal samples from calves in 10 other prefectures were examined by reverse transcription (rt)-pcr with primers designed in the spike (s) gene for the presence of botv. the specimens were also examined for the ... | 2007 | 17551218 |
| isolation of a megatrypanum trypanosome from sika deer (cervus nippon yesoensis) in japan. | a trypanosome was isolated from a sika deer (cervus nippon yesoensis) in hokkaido, japan, during the primary culture of sika deer renal cells. this is the first report of isolation of a megatrypanum trypanosome from japanese cervidae. the trypanosome, designated tsd1, was propagated and maintained in eagle's modified essential medium containing 20% fetal bovine serum with sika deer renal cells as feeder. the tsd1 trypanosome was morphometrically similar to trypanosoma cervi, which is commonly is ... | 2007 | 17714873 |
| adverse effects of ivermectin on the dung beetles, caccobius jessoensis harold, and rare species, copris ochus motschulsky and copris acutidens motschulsky (coleoptera: scarabaeidae), in japan. | effects of the antiparasitic drug, ivermectin, on the dung beetles, caccobius jessoensis harold, 1867 and the rare species, copris ochus motschulsky, 1860 and copris acutidens motschulsky, 1860 were studied in laboratory and field experiments in hokkaido, japan. ivermectin was detected in dung from 1 to 21 or 28 days following treatment, with a peak on the first day after treatment in two pour-on administrations (500 microg kg(-1)), although there were considerable differences between the two pe ... | 2007 | 17997876 |
| epidemiological study on bse outbreak in japan. | the aim of this study is to identify, as hypotheses, all feasible sources and routes of infection for the bse cases in japan, and to study the probability of each hypothesis. the strategy of this epidemiological study is as follows. 1) bse risk status in japan is tentatively divided into 3 stages, i.e., before 1996 april when administrative guidance for feed ban of cattle mbm introduced. after that to 2001 september, the first case of bse in japan, then, after 2001 october with real feed ban in ... | 2008 | 18460825 |
| molecular detection of mycoplasma wenyonii and 'candidatus mycoplasma haemobos' in cattle in hokkaido, japan. | blood samples from 78 cattle were tested for hemoplasma infection using molecular methods. pcr and sequence analysis revealed that 17 cattle were infected with mycoplasma wenyonii, while 13 were infected with 'candidatus mycoplasma haemobos'. four animals were infected with both species. this is the first study to report hemoplasma species infection among cattle in japan. | 2008 | 18571343 |
| serological and molecular survey of rickettsial infection in cattle and sika deer in a pastureland in hidaka district, hokkaido, japan. | rickettsial infection of cattle and sika deer from a pastureland in hidaka district, hokkaido, japan was examined by serological and molecular methods. serum samples from 8 of 83 (9.6%) cattle reacted with rickettsia helvetica antigens in an ifa test, with titers ranging from 1:160 to 1:640, while serum samples from 15 of 22 (68.2%) deer were positive for r. helvetica, with titers ranging from 1:80 to 1:640. in a genus-specific nested pcr based on glta, no cattle were positive for rickettsia, wh ... | 2008 | 18653979 |
| epidemiology of bovine spongiform encephalopathy in cattle in hokkaido, japan, between september 2001 and december 2006. | between october 2001 and december 2006 an estimated total of 6 million cattle in japan were tested for bse, with 31 returning a positive result. exploratory mapping, the space-time scan statistic, and ordinal logistic regression have been used to describe the epidemiology of the 24 cases identified in the prefecture of hokkaido, and to quantify the risk factors for the disease. two birth cohort groups were affected: cattle born during a period of seven months in 1996, and cattle born between 199 ... | 2008 | 19074787 |
| molecular survey for anaplasma bovis and anaplasma phagocytophilum infection in cattle in a pastureland where sika deer appear in hokkaido, japan. | anaplasma bovis and anaplasma phagocytophilum infection were examined by species-specific nested polymerase chain reaction in cattle in a pastureland where sika deer appear in hokkaido, japan. of the 78 cattle examined, 12 (15%) and 1 (1%) tested positive for infection by a. bovis and a. phagocytophilum, respectively. one cattle tested positive for both. a. phagocytophilum infection rates were significantly lower in cattle than in sika deer (46%), but the infection rate by a. bovis was not signi ... | 2009 | 19168967 |
| phylogenetic studies of bovine coronaviruses isolated in japan. | molecular analysis of the polymorphic region of the bovine coronavirus (bcov)-s gene using recent japanese field isolates and reference strains revealed that the 148 isolates collected from 1999 to 2008 from 13 prefectures, covering all regions of japan (hokkaido, tohoku, kanto, chubu, kinki, chugoku, shikoku, and kyusyu region) and divided into 3 clusters, show distinctive divergence from the prototype enteric bcov strains. almost all isolates after 2005 were clustered into group 4, and there w ... | 2009 | 19194080 |
| nongenetic effects and genetic parameters for length of productive life of holstein cows in hokkaido, japan. | records of holstein dairy cows in hokkaido, japan, were used to study the effects of environmental factors on length of productive life and to estimate genetic parameters for length of productive life. each record was assigned to 1 of 3 data sets depending on the percentage of type-scored cows in the herd. this percentage was considered to partly reflect the management policy in each herd, in particular regarding culling. the a, b, and c data sets consisted of herds with none, less than 60%, and ... | 2009 | 19389972 |
| molecular epidemiology of rotaviruses among healthy calves in japan: isolation of a novel bovine rotavirus bearing new p and g genotypes. | a total of 171 fecal specimens collected from healthy calves on a beef farm in gifu prefecture, japan in 2006-2007 were examined for group a rotaviruses by rt-semi-nested pcr targeting the coding region for vp8*. nine specimens were positive for rotavirus. g and p genotyping indicated that one strain was g10p[11]-like and six strains were considered to be the same unknown g and p genotypes. among these six untypeable strains, one strain, azuk-1, was adapted to cell culture and analyzed. sequence ... | 2009 | 19464329 |
| a retrospective survey of the prevalence of complex vertebral malformation carriers in 9 holstein dairy herds in hokkaido, japan. | the carrier rates of complex vertebral malformation (cvm) in 9 holstein dairy herds in hokkaido, number of usages of cvm carrier semen for breeding and gene frequencies of cvm carriers were measured. the mean cvm carrier rates of 140 cows from 4 herds in 1994 and 315 cows from 5 herds in 2003 were 10.8%(range 4.7-30.0%) and 5.1%(range 0.0-6.1%), respectively. the rate of use of cvm carrier semen in the hokkaido district was 5.6% in 2002. the gene frequencies calculated from cvm carriers among th ... | 2009 | 19578290 |
| relationships between reproductive traits of heifers and cows and yield traits for holsteins in japan. | the objective of this study was to investigate relationships between reproductive traits in heifers and cows and yield traits for holsteins in japan. insemination and lactation records for cows calved between 1990 and 2003 in hokkaido region were obtained. age at first service, age at conception, and conception rate for first service were calculated for heifers. days from calving to first service, days open, and conception rate for first service were calculated for first- and second-parity cows. ... | 2009 | 19620689 |
| epidemiological survey of theileria orientalis infection in grazing cattle in the eastern part of hokkaido, japan. | theileria orientalis is one of the benign species of theileria that is widely distributed in japan and is sometimes responsible for serious economic losses in the livestock industry. in the present study, we surveyed the current status of t. orientalis infection in grazing cattle in the eastern areas of hokkaido (taiki, otofuke, shintoku, and shin-hidaka districts) using molecular methods, as well as traditional methods, of diagnosis. the genes encoding the major piroplasm surface protein (mpsp) ... | 2009 | 19652482 |
| antimicrobial resistance and genetic characteristics of salmonella typhimurium isolated from horses in hokkaido, japan. | in this study, we examined the antimicrobial susceptibility of 16 salmonella typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (pcr) for detecting class 1 integrons, multiplex pcr for detecting multidrug resistant s. typhimurium definitive phage type 104 (mr-dt104), and fluorescent amplified-fragment length polymorphism (faflp). seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (acssut) t ... | 2009 | 19721370 |
| detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan. | dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ... | 2010 | 19897306 |
| a case control study of bovine viral diarrhea virus (bvdv) persistent infection (pi) in betsukai, hokkaido, japan. | the betsukai town office implemented bovine viral diarrhea virus (bvdv) preventive activities (i.e., vaccination and surveillance) in 2006. using bulk tank milk screening followed by individual blood tests using a reverse transcription-polymerase chain reaction (rt-pcr) method, persistent infection (pi) cattle were detected and eliminated from the population. based on data for pi cattle detected between 2006 and 2007, we conducted a case control study to find risk factors associated with the pre ... | 2010 | 20057177 |
| molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in japan. | this study reports the detection and genetic characterization of porcine kobuvirus, a new member of the genus kobuvirus in the family picornaviridae. among 293 fecal specimens collected from healthy pigs in 2009, in japan, 133 (45.4%) were positive for kobuvirus using rt-pcr screening method. of the positive specimens, 124 were obtained from pigs < or =6 months old, while 9 samples were from pigs >6 months old. fifty-two representative strains of kobuviruses detected in this study were randomly ... | 2010 | 20547246 |
| molecular detection of anaplasma phagocytophilum in cattle and ixodes persulcatus ticks. | the tick-borne pathogen, anaplasma phagocytophilum (a. phagocytophilum), the causative agent of human granulocytic anaplasmosis (hga), is increasingly becoming a public health concern as an aetiological agent for emerging infectious disease. we found a. phagocytophilum infection in a pooled sample of field-collected ixodes persulcatus (i. persulcatus) ticks from one district in hokkaido, japan. thus, to further investigate the prevalence in field-collected ticks, we used pcr assays targeting the ... | 2010 | 21146335 |
| sero-epidemiological analysis of vertical transmission relative risk of borna disease virus infection in dairy herds. | borna disease virus (bdv) is a virus that causes a neurological disease in domestic animals, including a variety of animal species in japan. few studies have examined the mode of transmission of this virus in cattle, and the exact mechanisms underlying the transmission of the virus need to be elucidated. this study aimed to examine the contribution of vertical transmission of the virus, which occurs when the virus is transmitted from the mother to offspring during gestation or birth. we used an ... | 2016 | 27498995 |
| molecular-based identification of yeasts isolated from bovine clinical mastitis in japan. | this study analyzed molecular-based identification of yeasts that associated with bovine clinical mastitis in japan. over 3,200 quarter milk samples from holstein dairy cows collected in 2011 on hokkaido and honshu islands were examined. yeast isolates were characterized by polymerase chain reaction amplification and sequencing of the d1/d2 region of the 26s rdna. molecular characterization confirmed that candida spp. and pichia spp. were most frequently isolated species. our molecular analysis ... | 2013 | 23100116 |
| molecular characterization of fasciola flukes obtained from wild sika deer and domestic cattle in hokkaido, japan. | the number of wild sika deer (cervus nippon yesoensis) continues to increase in hokkaido prefecture, japan. the major concern for the livestock industry is the transmission of pathogens between sika deer and cattle. fasciolosis is an important disease that can occur in both animals. the aim of this study was to examine the possible mutual transmission of this disease in hokkaido prefecture. a total of 105 fasciola flukes were obtained from sika deer and 96 from domestic cattle. the fasciola fluk ... | 2017 | 28396295 |
| the cumulative methane production from dairy cattle slurry can be explained by its volatile solid, temperature and length of storage. | in order to refine the national estimate of methane emission from stored cattle slurry, it is important to comprehend the basic characteristics of methane production. two dairy cattle slurries were obtained from livestock farms located in hokkaido (a northern island) and kyushu (a southern island). the slurries were diluted with water into three levels: undiluted, three times diluted, and 10 times diluted. three hundred ml of the slurries were put into a bottle with a headspace volume of 2.0 l, ... | 2016 | 26387490 |
| genetic correlations between production and disease traits during first lactation in holstein cows. | the aim of this study was to estimate genetic correlations between milk yield, somatic cell score (scs), mastitis, and claw and leg disorders (clds) during first lactation in holstein cows by using a threshold-linear random regression test-day model. we used daily records of milk, fat and protein yields; somatic cell count (scc); and mastitis and cld incidences from 46 771 first-lactation holstein cows in hokkaido, japan, that calved between 2000 and 2009. a threshold animal model for binary rec ... | 2014 | 24230485 |
| evaluation of fecal shedding and antibody response in dairy cattle infected with paratuberculosis using national surveillance data in japan. | paratuberculosis or johne's disease (jd), is a chronic infectious disease causing intractable diarrhea in cattle, which leads to less productivity, such as decreased milk yield, and lower daily weight gain. as a control measure against jd in cattle, national serological surveillance has been conducted in japan since 1998. to conduct modeling studies that are useful to evaluate the effectiveness of control measures against jd, reliable parameter values, such as length of time from infection to th ... | 2018 | 29290299 |
| herd-level risk factors associated with leptospira hardjo infection in dairy herds in the southern tohoku, japan. | a cross-sectional study was designed to generate information on the herd level prevalence and the risk factors for leptospira serovar hardjo (l. hardjo) in yamagata, the southern tohoku, japan. bulk tank milk samples from 109 dairy herds were used to test the herd level sero-prevalence of l. hardjo using a commercial elisa kit, which detects both l. interrogans serovar hardjo and l. borgpetersenii serovar hardjo. a questionnaire survey was conducted at the sampled farms, and univariable and mult ... | 2018 | 29290296 |
| epidemiological survey of border disease virus among sheep from northern districts of japan. | the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ... | 2011 | 21778667 |
| molecular epidemiology of salmonella enterica serovar typhimurium isolates from cattle in hokkaido, japan: evidence of clonal replacement and characterization of the disseminated clone. | the molecular epidemiology of 545 salmonella enterica serovar typhimurium isolates collected between 1977 and 2009 from cattle in hokkaido, japan, was investigated using pulsed-field gel electrophoresis (pfge). nine main clusters were identified from 116 pfge patterns. cluster i comprised 248 isolates, 243 of which possessed a sequence specific to definitive phage type 104 (dt104) or u302. the cluster i isolates were dominant in 1993 to 2003, but their numbers declined beginning in 2004. beginni ... | 2011 | 21239560 |
| epidemiological features of the bovine spongiform encephalopathy epidemic in japan. | on 10 september 2001, the first case of bovine spongiform encephalopathy (bse) in japan was confirmed in a five-year-old dairy cow born in hokkaido and raised in chiba prefecture. subsequently, bse surveillance was enhanced. as a result, 35 additional cases were detected by the end of march 2009, with two to ten cases being detected each year up to the end of 2007. the epidemic appeared to peak in 2006. cases are detected mostly in dairy cattle; in cattle born in 1996 and 2000; and in cattle bor ... | 2009 | 20462152 |
| an outbreak of teat papillomatosis in cattle caused by bovine papilloma virus (bpv) type 6 and unclassified bpvs. | out of 700 heifers at a local farm in hokkaido, the northern island of japan, 560 (80%) were found to have benign teat tumors. all of the analyzed tumors were macroscopically of the flat-and-round type, and no other types such as rice-grain or frond epithelial type were found. the lesions were characterized by epithelial hyperplasia, acanthosis and hyperkeratosis. unlike in typical fibropapilloma, fibroplasia of the underlying dermis was not observed. bovine papilloma virus (bpv) capsid antigen ... | 2007 | 17239550 |
| isolation of cryptosporidium andersoni kawatabi type in a slaughterhouse in the northern island of japan. | fecal samples were collected from 325 adult cattle and 108 pigs in a slaughterhouse in hokkaido, the northern island of japan. five adult cattle were found to be positive for oocysts of cryptopsoridium (1.5%). the oocysts were morphologically similar to those of cryptosporidium andersoni. the partial sequence of the 18s rrna gene of the isolate was 100% identical with that of the c. andersoni kawatabi strain. scid mice were infected after oral administration. based on the morphology of the oocys ... | 2005 | 15878239 |
| relationship of bovine viral diarrhea virus persistent infection to incidence of diseases on dairy farms based on bulk tank milk test by rt-pcr. | the prevalence of bovine viral diarrhea virus (bvdv) in dairy herds in hokkaido, japan, was estimated by reverse transcription polymerase chain reaction (rt-pcr) using bulk tank milk samples. sixteen out of 265 dairy herds were identified as bvdv positive, and at least one persistently infected (pi) cattle was recognized in each of the positive herds except for two herds of which, owners did not agree to examine individual cows. the proportion of positive herds with a history of bvdv pi was sign ... | 2005 | 15737472 |
| phylogenetic analysis of theileria sp. from sika deer, cervus nippon, in japan. | the 18s rrna genes of theileria species detected in sika deer, cervus nippon centralis in yamaguchi and cervus nippon yesoensis in hokkaido, were analyzed. the percent identities of the nucleotide sequences of theileria from cervus nippon centralis and cervus nippon yesoensis were more than 99%. the percent identities of the theileria sp. from sika deer and theileria sergenti, theileria buffeli and theileria cervi were 97, 96 and 95%, respectively. phylogenetic analysis of the gene sequences als ... | 2004 | 15063944 |
| cryptosporidium infection of cattle in the tokachi district, hokkaido. | the prevalence of cryptosporidium infection was examined in 480 healthy cattle (0-39 months old) in the tokachi district in hokkaido during the period from june to september in 2000 and from june to july in 2001. c. parvum oocysts were detected in 6 of 50 cattle (0-2 months old) in 2001; while c. muris was detected in 2 of 56 cattle (6-8 months old) in 2001, in 1 of 15 cattle (9-11 months old) in 2001, in 1 of 88 cattle (15-17 months old) in 2000, in 4 of 89 cattle (18-21 months old) in 2000 and ... | 2003 | 12576718 |
| seasonal change in the number of cryptosporidium parvum oocysts in water samples from the rivers in hokkaido, japan, detected by the ferric sulfate flocculation method. | an epidemiological study was carried out in natural water supplies of hokkaido, one of the largest dairy prefectures in japan. to investigate the prevalence of cryptosporidium parvum (c. parvum) oocysts water samples were collected from three rivers in the eastern area of hokkaido from august 1999 to october 2001, and c. parvum oocysts were collected and purified by the ferric sulfate flocculation method. the oocysts were detected using the immunofluorescent assay test (ifat) and 4', 6-diamidino ... | 2003 | 12576717 |
| distribution of antibody against erysipelothrix rhusiopathiae in cattle. | serum samples collected from 854 cattle in nine prefectures of japan, from hokkaido to okinawa, between 1988 and 1992 were examined for presence of antibodies against erysipelothrix rhusiopathiae by growth agglutination test. most of the sera showed positive reactions, and the antibody titers ranged from below 4 to above 128. seventy-six percent of the sera showed titers of 32 or above, and 34% showed titers of 128 or above. the titers had a tendency to be higher in the south and lower in the no ... | 2001 | 11329469 |
| antigenic and genetic diversities of babesia ovata in persistently infected cattle. | exploring the antigenic and genetic diversities of babesia ovata, we obtained several field isolates from grazing cattle in the okushiri island, japan. parasite isolation was greatly facilitated by using bovine red blood cell-substituted scid mice (bo-rbc-scid mice), into which the blood samples of the cattle were inoculated. isolates from different individuals within a herd of cattle were compared in immunoblot analysis with an anti-b. ovata serum and also in southern blot analysis with a probe ... | 1998 | 9879533 |
| prevalence and allele frequency estimation of bovine leukocyte adhesion deficiency (blad) in holstein-friesian cattle in japan. | blood samples from 796 holstein dairy cows in 20 herds from 6 districts in japan from june 1994 to august 1995 were examined to determine whether they were blad-free, blad carriers, or blad-affected by use of dna-polymerase chain reaction (pcr) analysis. the usage of semen of confirmed blad-carriers for artificial insemination in the hokkaido district and two selected dairy farms was examined to estimate the gene frequency of blad carriers of sires. blad-carrier prevalence in 20 herds (796 cows, ... | 1997 | 9152929 |
| high prevalence of borna disease virus infection in healthy sheep in japan. | previous seroepidemiological and molecular epidemiological studies of borna disease virus (bdv) showed considerably high rates of infection in horses, cattle, cats, and humans in hokkaido, japan. here, we further demonstrate high rates of specific antibodies to bdv and bdv rna in peripheral blood mononuclear cells (pbmcs) from healthy sheep bred on the same island. the bdv prevalences by immunoblotting and/or reverse transcriptase pcr were 0% (0 of 19) in newborns (<1 month old), 51.7% (15 of 29 ... | 1997 | 9144374 |
| seasonal variations in the presence of antibodies against coxiella burnetii in dairy cattle in hokkaido, japan. | the prevalence and seasonal variations of infection by coxiella burnetti in cattle were investigated seroepidemiologically on a farm in hokkaido, japan, by an immunofluorescent antibody test. a total of 364 serum samples from 28 cows were collected from august 1993 to october 1995 in two barns on the farm. it was found that the number of antibody-positive cows and their antibody titers were significantly elevated in winter and decreased in summer. in addition, antibodies were detectable in seroc ... | 1997 | 9087948 |
| [risk factors in serologically diagnosed echinococcosis--a case control study in the nemuro region of hokkaido prefecture]. | to determine the risk factors for echinococcus infection, we conducted a case-control study in the nemuro region of hokkaido prefecture. the cases included both clinically diagnosed patients with echinococcosis and registered persons who were seropositive. the control group was matched by residence, age (+/- 5 years) and sex. the data on the 99 cases and 430 controls were collected by mailed questionnaire. because of the long latency of echinococcosis, we investigated the potential risk factors ... | 1996 | 8755678 |
| morphological, serological and antigenic characteristics, and protein profile of newly isolated japanese bovine babesia parasite with particular reference to those of b. ovata. | an intraerythrocytic large protozoan, tentatively designated babesia sp. 1, was recently isolated from cattle in hokkaido prefecture, japan. this parasite closely resembled b. ovata in shape of piroplasms, but was distinguishable by other morphological, immunological, and biochemical characters. the paired pyriform piroplasm of b. sp. 1 was larger than that of b. ovata. the results from serological and antigenic examination by enzyme-linked immunosorbent assay (elisa) and western blot analysis s ... | 1995 | 8519896 |
| seroepizootiological survey on bluetongue virus infection in cattle in japan. | bovine sera collected in various parts of japan were subjected to seroepizootiological tests with bluetongue virus type 1 (btv1), type 12 (btv12), and type 20 (btv20). all these viruses have been widely disseminated among cattle in the southern part of japan in 1974. relatively high incidences of neutralizing (nt) antibody against the three viruses were shown among cattle in the kyushu district, including okinawa prefecture, or the southern part of japan, but extremely low or incidences in hokka ... | 1982 | 6312332 |
| studies on the cowpox-like disease outbreak in hokkaido, japan. ii. properties and identification of the isolates. | | 1964 | 5896579 |
| studies on the cowpox-like disease outbreak in hokkaido, japan. i. isolation of the causative agent. | | 1964 | 5896555 |
| a survey on salmonella in rectal swabs of cattle on dairy farms and steer feed lots in hokkaido. | | 1973 | 4546537 |
| [statistical study on the predisposition to summer sterility in holstein bulls born in hokkaido]. | | 1975 | 1142571 |