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y chromosomal haplotype characteristics of domestic sheep (ovis aries) in china.investigations on the variation present at the male-specific y chromosome region provide strong information to understand the origin and evolution of domestic sheep. one snp oy1 (g.88a>g) in the upstream region of sry gene, and the microsatellite srym18 locus within ovine y chromosome were analyzed in one hundred and forty five samples collected from eleven breeds in china. snp oy1 was analyzed using pcr-sscp method and sequencing. two different pcr-sscp patterns represented two specific sequenc ...201525865303
the complete mitochondrial genome sequence analysis of tibetan argali (ovis ammon hodgsoni): implications of tibetan argali and gansu argali as the same subspecies.the genus ovis (bovidae, artiodactyla) includes six species, i.e. ovis ammon, ovis aries, ovis canadensis, ovis dalli, ovis nivicola and ovis vignei. based on morphology, geographical location, habitat, etc., the species o. ammon is divided into nine subspecies. the near threatened tibetan argali is distributed across the tibetan plateau and its peripheral mountains, and believed to be one of the o. ammon subspecies (o. a. hodgsoni). however, considering its morphological features and distributi ...201323542075
an outbreak of sheep pox associated with goat poxvirus in gansu province of china.three strains of capripoxviruses (capvs) were isolated from an outbreak of sheep pox in gansu province of china. they were analyzed by p32 gene-based molecular methods and a species-specific pcr based on the rpo30 gene. two bands which are specific to goat poxvirus (gtpv) were observed after the pcr products of p32 gene were digested with the endonuclease of hinf i. moreover, an amplicon of 172bp, which is specific to gtpv, was amplified from the viruses by using the rpo30 gene-based pcr. sequen ...201122169434
mitochondrial dna diversity, origin, and phylogenic relationships of three chinese large-fat-tailed sheep breeds.china is abundant of sheep genetic resources. a total of 55 sequences containing the ovis aries mtdna d: -loop of three large-fat-tailed sheep breeds, named lanzhou, tong, and han were retrieved from genbank to investigate their genetic diversity, origin, and phylogenetic evolution. the results showed that the sheep breeds in our study proved to be extremely diverse, the average haplotype diversity and nucleotide diversity were 0.987 ± 0.006 and 0.03956 ± 0.00206, respectively. the 55 sequences ...201121503751
molecular phylogeography and genetic differentiation of blue sheep pseudois nayaurszechuanensis and pseudois schaeferi in china.within the genus pseudois (bovidae, artiodactyla), two challenges are faced by taxonomists. first, the designation of the two subspecies of blue sheep, pseudois nayaur nayaur and pseudois nayaurszechuanensis, tends to be questionable with the accumulation of morphological and molecular data. second, the taxonomic status of the dwarf blue sheep (pseudois schaeferi) has been controversial since its discovery, and molecular analyses with relatively small sample sizes have not painted a clear pictur ...200818565767
the development and evaluation of a loop-mediated isothermal amplification (lamp) method for detection of babesia spp. infective to sheep and goats in china.the loop-mediated isothermal amplification (lamp) reaction is a method that amplifies with high sensitivity, efficiency, and rapidity, deoxyribonucleic acid (dna) under isothermal condition in simple incubators. two primer sets for the lamp method were designed using the nucleotide sequences of 18s rrna gene of babesia sp. bq1 (lintan) and babesia sp. xinjiang-2005 isolated in china. the primers were used to detect parasite dna extracted from infected blood and purified parasites by lamp. the sp ...200818504039
serological investigation of ovine theileriosis by elisa in gannan tibet region of gansu province in china.the gannan tibet autonomous region is the biggest and most important stock-raising region of gansu province, people's republic of china. many sheep and goats from this region suffer from theileria challenge each year, resulting in inestimable economical losses. a first large-scale serological investigation by enzyme-linked immunosorbent assay of ovine theileriosis in this region was performed by testing a total of 1,165 sera samples collected from seven districts of three counties. the results s ...200717823828
comparative molecular characterization of gene segment 11-derived nsp6 from lamb rotavirus llr strain used as a human vaccine in china.sequence-length polymorphism is known for rotavirus genetic segment 11 (encodes non-structural protein, nsp6). with the exception of 11 strains that have the coding potential for a 98-residue nsp6, majority of the strains have the potential for a 92-residue nsp6. in nine strains, the coding potential for this protein is even shorter. this report focuses on the nsp6 gene nucleotide sequence of lanzhou lamb rotavirus (llr) strain and its comparative molecular characterization. the llr strain is a ...200616492399
[origin and genetic diversity of mongolian and chinese sheep using mitochondrial dna d-loop sequences].to determine the origin and gene diversity of the chinese and mongolian domestic sheep, a partial fragment of mitochondrial dna d-loop was sequenced for total number of 314 individuals from nine chinese sheep populations and 11 mongolian sheep populations. the results show no difference in nucleotide composition between chinese and mongolian sheep mtdna d-loop sequences. however, more variables were identified in mongolian sheep (26.85% of the sites) than that in chinese sheep (24.22%). in china ...200516459654
agricultural causes of desertification risk in minqin, china.this study uses statistical modeling techniques to develop a desertification risk index (ri) for minqin county, gansu province, china. twenty socio-economic factors were selected and compared with the ri results to explore the spatial and temporal variability of desertification risk in the study area and to identify possible local driving forces behind desertification risk. the explanatory factors were different in 1988, 1992 and 1997, possibly reflecting the role of temporal variation as a cont ...200616337079
molecular study of echinococcus in west-central china.west-central china is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with echinococcus granulosus and e. multilocularis . isolates of e. granulosus were collected from humans and other animals from different geographical areas of qinghai, ningxia, gansu and sichuan, and genotyped using the mitochondrial dna marker atp synthase subunit 6 gene (atp6). the sheep strain (g1 genotype) of e. granulosus was shown to ...200516174420
epidemiology of ovine theileriosis in ganan region, gansu province, china.the epidemiology of ovine theilreiosis in gannan tibet autonomous region was investigated. the results of these studies indicate that this disease is endemic in the following counties of gannan where the vector tick was identified as haemaphysalis qighaiensis: luqu, xiahe, hezuo, zhuoni, lintan, diebu, maqu. the disease mainly occurred from march to may and some cases also occurred in september and october. the incidence rates in lambs of sheep brought from areas free of the disease were high. o ...200212051605
isolation and preliminary characterization of a large babesia sp. from sheep and goats in the eastern part of gansu province, china.two species of babesia were isolated from naturally infected sheep in the eastern part of gansu province. one of the species was identified as b. ovis, while the another one seems as yet to be an unidentified babesia species. the latter could be transmitted transovarially by haemaphysalis longicornis. it was shown that larvae and nymphs transmit the organism to sheep and goats with a prepatent period of 5-28 days. as the carrier period of b. ovis is longer than that of the larger species and the ...200212051600
survey on cystic echinococcosis in tibetans, west china.human cystic echinococcosis (ce) is a serious public health problem in west china. the tibetan has a nature relation with these definitive and intermediate hosts in west china. although tibet is ce endemic, a few community studies have been reported. eight hundred and eighty two tibetans residing in the three rural communes of tianzhu county, gansu province of west china were investigated by serology and ultrasound scanning. 11.2% serum antibody positive rate in elisa test was found out, and a 1 ...200212039679
studies on the pathogenesis of shimao zheng (fleece-eating) in sheep and goats.research on the disease called shimao zheng, which had resulted in widespread fleece-eating and shedding by sheep and goats in the haizi area of akesa county of gansu province of china, was carried out by both laboratory studies and pathological observations. there was a marked deficiency of sulfur in the wool and tissues of the affected animals. the pathological changes were mainly loss of fleece, exposure of the skin, keratinization of the epidermal cells, narrowing and reduced numbers of the ...200111767009
an epidemiological survey of cystic echinococcosis among tibetan school pupils in west china.human cystic echinococcosis (ce) caused by infection with a larval stage of echinococcus granulosus is a serious public health problem in west china. because ce is a zoonosis, dogs and livestock are important hosts in transmission. tibetans, especially teenagers, have a natural relationship with these definitive and intermediate hosts in west china and although some areas of tibet are therefore ce-endemic, few, if any, detailed community studies have been reported. a total of 451 tibetan school ...200111579862
theileriosis of sheep and goats in china.theileriosis is an important disease of sheep and goats in west china. its main distribution includes qinghai, gansu, ningxia, inner mongolia, shaanxi and sichuan. the epidemic period is from late march to july with april-may being the peak months. this is the period of most intensive tick attack by haemaphysalis qinghaiensis (77.2-99.24%) during the year. it has been proved that the nymphs and adults, which develop from larvae and nymphs engorged on infected sheep or goats can transmit the path ...19979512737
serum biochemical values and mineral element contents of tissues in yaks.serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in shandan county of gansu province. the liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. the liver contained the highest concentrations of copper, manganese and cobalt and the kidney of sel ...19958619285
[investigations on the epidemiology of brucellosis in some villages (pasturelands) of su nan county, gansu province].during april-may 1991, by using sat. cft. and coomb's methods epidemiological surveillance on brucellosis among human beings and animals in some villages and pasturelands of su nan county was conducted. we found out that the serum brucella antibody was positive in 61 cases (19.06% out of 320 human beings) and 49 cases were diagnosed as brucellosis. we also made an investigation on 497 cattle and 1989 sheep with sat methods. the result showed that 15 sera showing a positive reaction all came from ...19938156577
[rflp analysis of dna from echinococcus granulosus collected from four provinces/autonomous region in china].a fragment of the ribosomal rna gene of schistosoma mansoni psm889 and two dna fragments specific to echinococcus granulosus phd5 and peg18 have been used as dna probes to assess the extent of genetic variability within e. granulosus. the dna analysis, including restriction endonuclease digestion and southern blot hybridization with the probes, did not demonstrate any genetic variation among e. granulosus collected from sheep in xinjiang, qinghai, gansu and ninxia. similarly, there was no geneti ...19937909504
studies on the relationship between sway disease of bactrian camels and copper status in gansu province.the clinical signs of camel sway disease in the hexi corridor of gansu province were defined. the contents of eight minerals in soils, in forage and in the blood and hair of bactrian camels from this region were determined. the related blood indices were also measured. the concentration of molybdenum in soils and forage was 4.8 +/- 0.02 and 4.8 +/- 0.25 micrograms/g (dry matter), respectively, the copper to molybdenum ratio in the forage being only 1.3. the concentration of copper in blood and h ...19947831754
epidemiological studies on cystic echinococcosis in china--a review.in the four decades from 1951 to 1990, the six provinces or autonomous regions (xinjiang, gansu, qinghai, ningxia, xizang and nei monggol) reported a total of 26,065 surgical cases of hydatid disease, most of which were reported in the recent decade. about one third of the patients was children and adolescents under 15 years old. so far, cystic hydatid infections of local origin have been confirmed in 22 provinces, autonomous regions and municipalities in the whole nation. findings of x-ray exam ...19957546341
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