cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ...200919732142
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