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pulsed-field gel electrophoresis applied for comparing listeria monocytogenes strains involved in outbreaks.recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by listeria monocytogenes serovar 4b strains. thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. we used pulsed-field gel electrophoresis of large chromosomal dna restriction fragments generated by apai, smai, or noti to analyse 75 l. monocytogenes strains isolated during six major and eight sm ...19938500010
absence of serotype-specific surface antigen and altered teichoic acid glycosylation among epidemic-associated strains of listeria monocytogenes.outbreaks of food-borne listeriosis have often involved strains of serotype 4b. examination of multiple isolates from three different outbreaks revealed that ca. 11 to 29% of each epidemic population consisted of strains which were negative with the serotype-specific monoclonal antibody c74.22, lacked galactose from the teichoic acid of the cell wall, and were resistant to the serotype 4b-specific phage 2671.200011015420
does sporadic listeria gastroenteritis exist? a 2-year population-based survey in nova scotia, canada.febrile gastroenteritis due to listeria monocytogenes (lm) has been primarily described in foodborne outbreaks. we decided to determine the incidence of sporadic, febrile gastroenteritis due to lm in a large, well-defined north american population over a 2-year period and to compare these cases to sporadic cases of campylobacter and salmonella infections occurring concurrently in the community.200516107973
comparison of the prevalences and diversities of listeria species and listeria monocytogenes in an urban and a rural agricultural watershed.foods and related processing environments are commonly contaminated with the pathogenic listeria monocytogenes. to investigate potential environmental reservoirs of listeria spp. and l. monocytogenes, surface water and point source pollution samples from an urban and a rural municipal water supply watershed in nova scotia, canada, were examined over 18 months. presumptive listeria spp. were cultured from 72 and 35% of rural and urban water samples, respectively, with 24% of the positive samples ...201525819965
surveillance of listeria monocytogenes in human and environmental specimens in nova scotia, 1974 to 1981.we examined specimens for l. monocytogenes using the "cold enrichment" technique of gray et al. (j. bacteriol., 55: 471, 1948) and a nalidixic agar plate (ann. inst. pasteur 111: 90, 1966). between 1974 and may 1981, we isolated l. monocytogenes from four of 5,255 specimens (rectal, vaginal and placental swabs; blood; spinal fluid; semen; necropsy material) which came from eight human populations (neonates, children, adult men, and pregnant and nonpregnant women) and from 161 animals. three of t ...19846442655
listeria monocytogenes infections in canada.since its first isolation by murray in 1926 listeria monocytogenes has become recognized as a significant pathogen occurring worldwide and involving a wide range of wild and domestic animals including man. the first confirmed human listeriosis case in canada was published by stoot in 1951. a later survey based on records maintained at the laboratory centre for disease control, ottawa showed 101 cases detected over a 21 year period in nine provinces. the overall mortality was 30%. the most freque ...19846442654
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