| analysis of open reading frame 5 in japanese porcine reproductive and respiratory syndrome virus isolates by restriction fragment length polymorphism. | for the epidemiological survey of porcine reproductive and respiratory syndrome virus (prrsv) isolated in japan, the open reading frame (orf) 5 gene of 37 field isolates in chiba prefecture from 1991 to 1999 were analyzed by restriction fragment length polymorphism (rflp). reverse tanscription-polymerase chain reaction (rt-pcr) amplifying the orf 5 gene detected 35 field isolates except for 2 isolates. rflp analysis with mlui, hincii, sacii, haeiii and mspi demonstrated that 35 field isolates we ... | 2001 | 11767054 |