| complete genome sequence of an isolate of bluetongue virus serotype 2, demonstrating circulation of a western topotype in southern india. | bluetongue virus serotype 2 (ind2003/02) was isolated in tiruneveli city, tamil nadu state, india, and is stored in the orbivirus reference collection at the institute for animal health, pirbright, united kingdom. the entire genome of this isolate was sequenced, showing that it is composed of a total of 19,203 bp (all 10 genome segments). this is the first report of the entire genome sequence of a western strain of btv-2 isolated in india, indicating that this virus has been introduced and is ci ... | 2012 | 22492927 |
| dual infection with bluetongue virus serotypes and first-time isolation of serotype 5 in india. | bluetongue is endemic in india and has been reported from most indian states. of late, the clinical disease is most frequently seen in the states of andhra pradesh, telangana (erstwhile andhra pradesh state), tamil nadu and karnataka. our analysis of diagnostic samples from bluetongue outbreaks during 2010-2011 from the state of karnataka identified bluetongue virus (btv) serotype 5 (btv-5) for the first time in india. one of the diagnostic samples (ch1) and subsequent virus isolate (ind2010/02) ... | 2016 | 28004522 |
| isolation of bluetongue virus serotype 1 (btv-1) from goats and its phylogenetic relationship to other btv-1 isolates worldwide based on full-length sequence of genome segment-2. | eight bluetongue viruses (btv) were isolated in bhk-21 cell culture from blood of goats suffering from peste des petits ruminants. these viruses were identified as btv serotype 1 (btv-1) by rt-pcr using vp2-gene-based primers coupled with sequencing of the pcr products. all of the isolates showed similar genome migration profile in 8% polyacrylamide gel electrophoresis. the genome segment-2 (seg-2) of one isolate (mkd18/india/2008) was amplified piecemeal by overlapping pcr, and the products wer ... | 2010 | 20842392 |
| occurrence of bluetongue in ruminants in tamil nadu, south india. | tamil nadu is located in the south-eastern part of indian peninsula, between 8.087° and 13.09°n and 76.50° and 80.27°e. bluetongue (bt) was first reported in this region in sheep during 1982 with regular occurrence thereafter. in 1989-1990, 1997-1998 and 2005-2006, there was wide spread occurrence of bt resulting in huge mortality of sheep. the present study had the goal of isolating the btv from outbreaks in sheep occurred in tamil naadu between 2003-2011 and comparing the vp2 gene sequences of ... | 2016 | 27723039 |
| vp2 gene based phylogenetic relationship of indian isolates of bluetongue virus serotype 1 and other serotypes from different parts of the world. | bluetongue virus (btv), a member of genus orbivirus, family reoviridae, is non-enveloped with double shelled structure and 10 segmented double stranded rna genome. the rna segment l2 encodes an outer capsid serotype specific viral protein vp2. btv serotype 1 (btv-1) specific novel primer pair, forward primer (1240-1271 bp) and reverse primer (1844-1813 bp), was designed using vp2 gene sequences available in genbank to amplify 1240-1844 bp region because two hypervariable and three conserved regi ... | 2004 | 15621660 |