Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsy specimens from white-tailed deer by real-time quaking-induced conversion.chronic wasting disease (cwd), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in colorado and wyoming and has since spread to cervids in 23 states, two canadian provinces, and the republic of korea. the expansion of this disease makes the development of sensitive diagnostic assays and antemortem sampling techniques crucial for the mitigation of its spread; this is especially true in cases of relocation/reintroduction of farmed or free-ranging deer ...201626865693
chronic wasting disease in white-tailed deer: infection, mortality, and implications for heterogeneous transmission.chronic wasting disease (cwd) is a fatal neurodegenerative disease affecting free-ranging and captive cervids that now occurs in 24 u.s. states and two canadian provinces. despite the potential threat of cwd to deer populations, little is known about the rates of infection and mortality caused by this disease. we used epidemiological models to estimate the force of infection and disease-associated mortality for white-tailed deer in the wisconsin and illinois cwd outbreaks. models were based on a ...201627870037
horizontal transmission of chronic wasting disease in reindeer.we challenged reindeer by the intracranial route with the agent of chronic wasting disease sourced from white-tailed deer, mule deer, or elk and tested for horizontal transmission to naive reindeer. reindeer were susceptible to chronic wasting disease regardless of source species. horizontal transmission occurred through direct contact or indirectly through the environment.201627869594
modeled impacts of chronic wasting disease on white-tailed deer in a semi-arid environment.white-tailed deer are a culturally and economically important game species in north america, especially in south texas. the recent discovery of chronic wasting disease (cwd) in captive deer facilities in texas has increased concern about the potential emergence of cwd in free-ranging deer. the concern is exacerbated because much of the south texas region is a semi-arid environment with variable rainfall, where precipitation is strongly correlated with fawn recruitment. further, the marginally pr ...201627711208
assessment of the prpc amino-terminal domain in prion species barriers.chronic wasting disease (cwd) in cervids and bovine spongiform encephalopathy (bse) in cattle are prion diseases that are caused by the same protein-misfolding mechanism, but they appear to pose different risks to humans. we are interested in understanding the differences between the species barriers of cwd and bse. we used real-time, quaking-induced conversion (rt-quic) to model the central molecular event in prion disease, the templated misfolding of the normal prion protein, prp(c), to a path ...201627654299
chronic wasting disease drives population decline of white-tailed deer.chronic wasting disease (cwd) is an invariably fatal transmissible spongiform encephalopathy of white-tailed deer, mule deer, elk, and moose. despite a 100% fatality rate, areas of high prevalence, and increasingly expanding geographic endemic areas, little is known about the population-level effects of cwd in deer. to investigate these effects, we tested the null hypothesis that high prevalence cwd did not negatively impact white-tailed deer population sustainability. the specific objectives of ...201627575545
primary transmission of chronic wasting disease versus scrapie prions from small ruminants to transgenic mice expressing ovine or cervid prion protein.development of mice expressing either ovine (tg338) or cervid (tgelk) prion protein (prp) have aided in characterization of scrapie and chronic wasting disease (cwd), respectively. experimental inoculation of sheep with cwd prions has demonstrated the potential for interspecies transmission but, infection with cwd versus classical scrapie prions may be difficult to differentiate using validated diagnostic platforms. in this study, mouse bioassay in tg338 and tgelk was utilized to evaluate transm ...201627393736
when mechanism matters: bayesian forecasting using models of ecological diffusion.ecological diffusion is a theory that can be used to understand and forecast spatio-temporal processes such as dispersal, invasion, and the spread of disease. hierarchical bayesian modelling provides a framework to make statistical inference and probabilistic forecasts, using mechanistic ecological models. to illustrate, we show how hierarchical bayesian models of ecological diffusion can be implemented for large data sets that are distributed densely across space and time. the hierarchical baye ...201728371055
pathways of prion spread during early chronic wasting disease in deer.among prion infections, two scenarios of prion spread are generally observed: (a) early lymphoid tissue replication or (b) direct neuroinvasion without substantial antecedent lymphoid amplification. in nature, cervids are infected with chronic wasting disease (cwd) prions by oral and nasal mucosal exposure, and studies of early cwd pathogenesis have implicated pharyngeal lymphoid tissue as the earliest sites of prion accumulation. however, knowledge of chronological events in prion spread during ...201728250130
endogenous brain lipids inhibit prion amyloid formation in vitro.the normal cellular prion protein (prp(c)) resides in detergent-resistant outer membrane lipid rafts in which conversion to the pathogenic misfolded form is believed to occur. once misfolding occurs, the pathogenic isoform polymerizes into highly stable amyloid fibrils. in vitro assays have demonstrated an intimate association between prion conversion and lipids, specifically phosphatidylethanolamine, which is a critical cofactor in the formation of synthetic infectious prions. in the current wo ...201728202758
molecular mechanisms of chronic wasting disease prion propagation.prion disease epidemics, which have been unpredictable recurrences, are of significant concern for animal and human health. examples include kuru, once the leading cause of death among the fore people in papua new guinea and caused by mortuary feasting; bovine spongiform encephalopathy (bse) and its subsequent transmission to humans in the form of variant creutzfeldt-jakob disease (vcjd), and repeated examples of large-scale prion disease epidemics in animals caused by contaminated vaccines. the ...201728193766
chronic wasting disease prion infection of differentiated neurospheres.a possible strategy to develop more diverse cell culture systems permissive to infection with naturally occurring prions is to exploit culture of neurospheres from transgenic mice expressing the normal prion protein (prp) of the native host species. accordingly, we developed differentiated neurosphere cultures from the cervid prp-expressing mice to investigate whether this in vitro system would support replication of non-adapted cervid-origin chronic wasting disease (cwd) prions. here we report ...201728762865
temporal patterns of chronic wasting disease prion excretion in three cervid species.chronic wasting disease (cwd) is the only naturally occurring transmissible spongiform encephalopathy affecting free-ranging wildlife populations. transmission of cwd occurs by direct contact or through contaminated environments; however, little is known about the temporal patterns of cwd prion excretion and shedding in wild cervids. we tested the urine and faeces of three species of captive cervids (elk, mule and white-tailed deer) at 6, 12, 18 and 24 months after oral inoculation to evaluate t ...201728708047
Displaying items 101 - 113 of 113