Publications

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diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting disease within white-tailed deer (odocoileus virginianus) herds in north america: effects of age, sex, polymorphism at prnp codon 96, and disease progression.an effective live animal diagnostic test is needed to assist in the control of chronic wasting disease (cwd), which has spread through captive and wild herds of white-tailed deer (odocoileus virginianus) in canada and the united states. in the present study, the diagnostic accuracy of rectal mucosa biopsy sample testing was determined in white-tailed deer from 4 cwd-infected captive herds. specifically, the current study compared the immunohistochemical detection of disease-associated prion prot ...201222914819
the walk is never random: subtle landscape effects shape gene flow in a continuous white-tailed deer population in the midwestern united states.one of the pervasive challenges in landscape genetics is detecting gene flow patterns within continuous populations of highly mobile wildlife. understanding population genetic structure within a continuous population can give insights into social structure, movement across the landscape and contact between populations, which influence ecological interactions, reproductive dynamics or pathogen transmission. we investigated the genetic structure of a large population of deer spanning the area of w ...201222882236
modeled impacts of chronic wasting disease on white-tailed deer in a semi-arid environment.white-tailed deer are a culturally and economically important game species in north america, especially in south texas. the recent discovery of chronic wasting disease (cwd) in captive deer facilities in texas has increased concern about the potential emergence of cwd in free-ranging deer. the concern is exacerbated because much of the south texas region is a semi-arid environment with variable rainfall, where precipitation is strongly correlated with fawn recruitment. further, the marginally pr ...201627711208
endogenous brain lipids inhibit prion amyloid formation in vitro.the normal cellular prion protein (prp(c)) resides in detergent-resistant outer membrane lipid rafts in which conversion to the pathogenic misfolded form is believed to occur. once misfolding occurs, the pathogenic isoform polymerizes into highly stable amyloid fibrils. in vitro assays have demonstrated an intimate association between prion conversion and lipids, specifically phosphatidylethanolamine, which is a critical cofactor in the formation of synthetic infectious prions. in the current wo ...201728202758
pathways of prion spread during early chronic wasting disease in deer.among prion infections, two scenarios of prion spread are generally observed: (a) early lymphoid tissue replication or (b) direct neuroinvasion without substantial antecedent lymphoid amplification. in nature, cervids are infected with chronic wasting disease (cwd) prions by oral and nasal mucosal exposure, and studies of early cwd pathogenesis have implicated pharyngeal lymphoid tissue as the earliest sites of prion accumulation. however, knowledge of chronological events in prion spread during ...201728250130
when mechanism matters: bayesian forecasting using models of ecological diffusion.ecological diffusion is a theory that can be used to understand and forecast spatio-temporal processes such as dispersal, invasion, and the spread of disease. hierarchical bayesian modelling provides a framework to make statistical inference and probabilistic forecasts, using mechanistic ecological models. to illustrate, we show how hierarchical bayesian models of ecological diffusion can be implemented for large data sets that are distributed densely across space and time. the hierarchical baye ...201728371055
prion protein polymorphisms affect chronic wasting disease progression.analysis of the prnp gene in cervids naturally infected with chronic wasting disease (cwd) suggested that prnp polymorphisms affect the susceptibility of deer to infection. to test this effect, we orally inoculated 12 white-tailed deer with cwd agent. three different prnp alleles, wild-type (wt; glutamine at amino acid 95 and glycine at 96), q95h (glutamine to histidine at amino acid position 95) and g96s (glycine to serine at position 96) were represented in the study cohort with 5 wt/wt, 3 wt/ ...201121445256
host culling as an adaptive management tool for chronic wasting disease in white-tailed deer: a modelling study.emerging wildlife diseases pose a significant threat to natural and human systems. because of real or perceived risks of delayed actions, disease management strategies such as culling are often implemented before thorough scientific knowledge of disease dynamics is available. adaptive management is a valuable approach in addressing the uncertainty and complexity associated with wildlife disease problems and can be facilitated by using a formal model.we developed a multi-state computer simulation ...200919536340
b cells and platelets harbor prion infectivity in the blood of deer infected with chronic wasting disease.substantial evidence for prion transmission via blood transfusion exists for many transmissible spongiform encephalopathy (tse) diseases. determining which cell phenotype(s) is responsible for trafficking infectivity has important implications for our understanding of the dissemination of prions, as well as their detection and elimination from blood products. we used bioassay studies of native white-tailed deer and transgenic cervidized mice to determine (i) if chronic wasting disease (cwd) bloo ...201020219916
chronic wasting disease in a wisconsin white-tailed deer farm.in september 2002, chronic wasting disease (cwd), a prion disorder of captive and wild cervids, was diagnosed in a white-tailed deer (odocoileus virginianus) from a captive farm in wisconsin. the facility was subsequently quarantined, and in january 2006 the remaining 76 deer were depopulated. sixty animals (79%) were found to be positive by immunohistochemical staining for the abnormal prion protein (prp(cwd)) in at least one tissue; the prevalence of positive staining was high even in young de ...200818776116
chronic wasting disease prion infection of differentiated neurospheres.a possible strategy to develop more diverse cell culture systems permissive to infection with naturally occurring prions is to exploit culture of neurospheres from transgenic mice expressing the normal prion protein (prp) of the native host species. accordingly, we developed differentiated neurosphere cultures from the cervid prp-expressing mice to investigate whether this in vitro system would support replication of non-adapted cervid-origin chronic wasting disease (cwd) prions. here we report ...201728762865
prion sequence polymorphisms and chronic wasting disease resistance in illinois white-tailed deer (odocoileus virginianus).nucleic acid sequences of the prion gene (prnp) were examined and genotypes compiled for 76 white-tailed deer from northern illinois, which previously tested positive for chronic wasting disease (cwd), and 120 negative animals selected to control for geographic location and age. nine nucleotide polymorphisms, seven silent and two coding, were found in the sampled population. all observed polymorphisms except two of very low frequency were observed in both negative and positive animals, although ...200819164895
wasting and neurologic signs in a white-tailed deer (odocoileus virginianus) not associated with abnormal prion protein.a captive adult male white-tailed deer (odocoileus virginianus) with wasting and neurologic signs similar to chronic wasting disease (cwd) was evaluated by histopathology, histochemistry, and immunohistochemistry (ihc) for disease-associated prion protein (prp(d)). on histologic examination, the brainstem had areas of vacuolation in neuropil and extensive multifocal mineralization of blood vessels with occasional occlusion of the lumen. some of the clinical and pathologic features of this case w ...200818957666
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