sequence heterogeneity among human picobirnaviruses detected in a gastroenteritis outbreak.human picobirnaviruses characterised in this study were serendipitously detected in a non-bacterial gastroenteritis outbreak when specimens were examined for the presence of human rotaviruses using polyacrylamide gel electrophoresis. of ten stool samples sent for virological examination, two, three, and one specimens were positive for human caliciviruses, picobirnaviruses, and both viruses, respectively. partial sequences of the rna-dependent rna polymerase gene were determined for three picobir ...200314648286
molecular epidemiology of human picobirnaviruses among children of a slum community in kolkata, india.picobirnaviruses are a group of unclassified, non-enveloped, small spherical viruses, 35-41 nm in diameter without any apparent surface morphology. they have characteristic bisegmented double stranded rna genome of two types namely large profile (2.3-2.6 kbp for the larger and 1.5-1.9 kbp for the smaller segment, respectively) or small profile (1.75 and 1.55 kbp for segments 1 and 2, respectively). human picobirnaviruses (n=12 positives; 2/56 diarrhoeic children and 10/607 non-diarrhoeic childre ...200616616879
detection of viral agents in fecal specimens of monkeys with diarrhea.diarrheal disease is a major cause of morbidity and mortality in humans and animals, including non human primates. while the diagnostics for gastrointestinal bacterial and parasitic pathogens and their etiological role in disease are well established, little is known about the epidemiology, prevalence and role of viral agents in diarrheal illness among monkeys.200717493140
genogroup i picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains.picobirnaviruses (pbvs) are small, non-enveloped viruses with a bisegmented double-stranded rna genome. their pathogenic potential, ecology, and evolutionary features are largely unexplored. here, we describe the molecular analysis of porcine pbvs identified in the intestinal content of dead pigs. six of 13 positive samples were cloned and then subjected to single-strand conformation polymorphism analysis and nucleotide sequencing. all clones belonged to genogroup i pbvs and almost all clones cl ...200818198385
molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (pbv) strain: evidence for high genetic diversity with genogroup i pbvs.we report here the molecular characterization of a bovine genogroup i picobirnavirus strain rubv-p detected from a 1-month-old diarrhoeic calf in eastern india. sequence comparisons and phylogenetic analysis of a short stretch of gene segment 2 of rubv-p revealed low nucleotide identities (51.2-64.9%) with and distant genetic relatedness to other genogroup i picobirnaviruses. the complete gene segment 2 sequence of rubv-p was obtained by the single primer amplification method with modifications. ...200919587136
picobirnaviruses encode a protein with repeats of the exxrxnxxxe motif.picobirnaviruses possess a bisegmented double-stranded rna genome. while the segment 2 encodes the rna-dependent rna polymerase, the segment 1 displays two open reading frames (orfs). orf2 was recently shown to code the capsid precursor and orf1 product has not been characterized. in this study, we show that the three orf1 sequences available in databases and representing three phylogenetically distant picobirnaviruses (two from human and one from rabbit hosts) encode proteins of various sizes ( ...201121376090
genogroup i and ii picobirnaviruses in respiratory tracts of pigs.sequence-independent amplification and specific reverse transcription pcrs identified genogroup i and ii picobirnaviruses in respiratory tracts of pigs. these data expand knowledge of picobirnavirus diversity and tropism. genetic relationships between porcine respiratory and human enteric picobirnaviruses suggest cross-species transmission of picobirnaviruses between pigs and humans.201122172405
detection and characterisation of bisegmented double-stranded rna viruses (picobirnaviruses) in human faecal specimens.the prevalence of picobirnaviruses (pbvs) in human stools was investigated by polyacrylamide gel electrophoresis (page) analysis of 832 faecal specimens collected between 1982 and 1993 from patients in various clinical groups. similar prevalences (9-13%) were detected in patients with or without gastroenteritis and throughout the age range of 3 to > 65 years. two methods for the extraction of nucleic acid, a phenol/chloroform method and a guanidinium thiocynate (gtc)/silica method, were compared ...19957775930
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