in vitro cultivation of gymnophalloides seoi metacercariae (digenea:gymnophallidae).gymnophalloides seoi is a human intestinal trematode prevalent on southwestern islands in korea. in the present study, we investigated whether g. seoi metacercariae can grow and develop into adults by in vitro cultivation. the metacercariae were obtained from naturally infected oysters, and cultured in vitro for 5 days under three conditions; 37 degrees c/5% co2, 41 degrees c/8% co2, or 41 degrees c/5% co2, in nctc 109 complete media containing 20% fbs and 1% antibiotics-antimycotics. the degree ...19979100436
parasitic infections in hiv-infected patients who visited seoul national university hospital during the period 1995-2003.the prevalence of parasitic infections was investigated in human immunodeficiency virus (hiv)-infected patients (n = 105) who visited seoul national university hospital, seoul, korea, during the period from 1995 to 2003. fecal samples were collected from 67 patients for intestinal parasite examinations, and sputum or bronchoalveolar lavage samples from 60 patients for examination of pneumocystis carinii. both samples were obtained from 22 patients. thirty-three (31.4%) of the 105 were found to h ...200515793352
isolation and characterization of a 40 kda cysteine protease from gymnophalloides seoi adult worms.a 40 kda cysteine protease was purified from the crude extract of adult worms of gymnophalloides seoi by two consecutive steps: sephacryl s-200 hr and deae-sephacel chromatography. enzyme activities were completely inhibited by cysteine protease inhibitors. l-trans-epoxysuccinylleucylamido (4-guanidino) butane (e-64) and iodoacetic acid, strongly suggesting that the purified enzyme belongs to the cysteine family of proteases. the enzyme was maximally active at ph 4.5 in 0.1 m of buffer, and its ...19989637831
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