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ribotyping characterisation of campylobacter isolates randomly collected from different sources in italy.in this study the potential for using the automated psti ribotyping as a primary library typing method to survey campylobacter and for identification of two thermophilic campylobacter jejuni and campylobacter coli species was evaluated. a total of 158 isolates randomly collected in italy from different sources were analyzed. a large percentage of chicken (28%), turkey (27%) and turkey meat (25%) isolates shared their ribotyping profiles (ribotypes) with those of humans, whereas the swine isolate ...200314522511
quinolone-resistant campylobacter infections: risk factors and clinical consequences.we integrated data on quinolone and macrolide susceptibility patterns with epidemiologic and typing data from campylobacter jejuni and c. coli infections in two danish counties. the mean duration of illness was longer for 86 patients with quinolone-resistant c. jejuni infections (median 13.2 days) than for 381 patients with quinolone-sensitive c. jejuni infections (median 10.3 days, p = 0.001). foreign travel, eating fresh poultry other than chicken and turkey, and swimming were associated with ...200415207057
occurrence of campylobacter in retail foods in ireland.a surveillance study was carried out to determine the prevalence of campylobacter in a range of retail foods purchased in three irish cities over a 20-month period between march 2001 and october 2002. in total 2391 food samples were analysed during this period. campylobacter was isolated from 444 raw chicken (49.9%), 33 turkey (37.5%) and 11 duck samples (45.8%). lower isolation rates of 7/221 (3.2%), 10/197 (5.1%) and 31/262 (11.8%) were observed for raw beef, pork and lamb, respectively. one s ...200415282123
genotyping of campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping.to determine the genetic relatedness of campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed-field gel electrophoresis (pfge) and automatic ribotyping.200616405698
bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults.campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ...200616977842
campylobacter genotyping to determine the source of human infection.campylobacter species cause a high proportion of bacterial gastroenteritis cases and are a significant burden on health care systems and economies worldwide; however, the relative contributions of the various possible sources of infection in humans are unclear.200919275496
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
antimicrobial resistance and molecular subtyping of campylobacter jejuni and campylobacter coli from retail meats.campylobacter isolates (n = 297; 202 c. jejuni and 95 c. coli isolates) recovered from 2,513 retail meat samples (chicken breasts, ground turkey, ground beef, and pork chops) were examined for antimicrobial susceptibility. the isolates were further analyzed for genetic relatedness by pulsed-field gel electrophoresis (pfge) using smai and kpni restriction enzymes, and a subset of isolates (n = 174) were subtyped by multilocus sequence typing (mlst). the resistance most frequently observed was tha ...201121477476
microbiological safety of retail vacuum-packed and modified-atmosphere-packed cooked meats at end of shelf life.a study of retail modified-atmosphere-packed and vacuum-packed cooked ready-to-eat meats was undertaken from september through mid-november 2003 to determine the microbiological quality at the end of shelf life and to establish any risk factors in the production, storage, and display of this product. examination of 2,981 samples using microbiological guidelines criteria revealed that 66% were of satisfactory or acceptable microbiology quality, 33% were of unsatisfactory quality mainly due to hig ...200717477265
antimicrobial resistance among campylobacter jejuni isolated from raw poultry meat at retail level in denmark.campylobacter jejuni isolated from raw poultry meat collected at retail shops in denmark in the period 1996-2003 were tested for susceptibility to seven antimicrobial agents. the food samples consisted of raw chicken meat and other raw poultry meat of domestic or imported origin. the highest levels of resistance among c. jejuni were observed for tetracycline, nalidixic acid and ciprofloxacin, whereas macrolide resistance was rarely detected. c. jejuni originating from other poultry meat (mainly ...200616410028
detection of seven virulence and toxin genes of campylobacter jejuni isolates from danish turkeys by pcr and cytolethal distending toxin production of the isolates.a total of 117 campylobacter jejuni isolates from danish turkeys were tested for the presence of seven virulence and toxin genes by pcr. one hundred seventeen (100%) isolates were positive for flaa, cadf, and ceue gene primers. one hundred three (88%) isolates were positive for cdt gene cluster pcr detection (cdt gene cluster-pcr), whereas 101 (86.3%), 102 (87.2%), and 110 (94%) isolates were positive for cdta-, cdtb-, and cdtc-pcr, respectively. only 39 (33.3%) isolates were positive for virb11 ...200415508626
repeated therapeutic dosing selects macrolide-resistant campylobacter spp. in a turkey facility.this study assessed the effects of the therapeutic use of tylan® in a large-scale turkey production facility on the selection of macrolide-resistant campylobacter.201020497488
evaluation of transport and storage techniques for isolation of campylobacter fetus subsp. jejuni from turkey cecal specimens.immediate culturing of fecal specimens is not always possible, and appropriate methods for transport and storage of campylobacter fetus subsp. jejuni specimens have not been fully evaluated. using nine techniques, we studied the survival of c. fetus subsp. jejuni in cecal specimens from infected turkeys. the organisms survived in specimens held without transport medium for 3 to 15 days (median, 9 days) at 4 degrees c, and 2 to 9 days (median, 4 days) at 25 degrees c. only 20% of specimens frozen ...19817016895
dna diversity among isolates of campylobacter jejuni detected by pcr-based rapd fingerprinting.a pcr-based randomly amplified polymorphic dna method was used to amplify campylobacter jejuni dna using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum. the method was able to detect the heterogeneity of amplified dna from human, chicken and turkey sources and can be used as a tool to study the epidemiology of campylobacter jejuni infection.19957571378
natural and experimental infections of arcobacter in poultry.arcobacter butzleri causes human enteritis and is frequently recovered from poultry carcasses. the purpose of this study was to determine 1) the natural distribution of a. butzleri in poultry and 2) its relative pathogenicity in experimentally infected poultry. cloacal samples (n = 407) were collected on four occasions from three flocks of chickens. overall, arcobacter spp. were recovered from 15% of the birds; a. butzleri was identified in 1% of cloacal samples. three experimental trials were c ...199910230906
campylobacter infection of commercial poultry.campylobacter jejuni, a widespread food-borne pathogen is responsible for enteritis in the populations of both industrialised and developing nations and is acquired by consumption of contaminated water, milk and food products. contaminated poultry meat is regarded as an important source of campylobacteriosis, with both commercial broiler and turkey growing flocks infected at two to three weeks of age by direct and indirect horizontal exposure. non-chlorinated water is regarded as a vehicle of in ...200010935269
pathogenicity of campylobacter jejuni for turkeys and chickens.a campylobacter jejuni isolate obtained from a turkey liver, designated c101, and a c. jejuni isolate obtained from the feces of a chicken, designated c111, were used to inoculate their respective hosts. isolate c101 depressed weight gain by 20% when inoculated into newly hatched poults or 4-day-old poults. it also caused death, hepatic necrosis, and generalized hemorrhages in turkey embryos. the chicken-derived isolate, c111, did not reduce weight gain in newly hatched chicks, but it did induce ...19921627109
[campylobacter jejuni gastroenteritis].acute gastroenteritis is a major cause of hospital admission with attendant morbidity and occasional mortality. campylobacter jejuni recently has been recognized as a common cause of acute bacterial gastroenteritis in children and adults. its frequency of isolation is comparable to and in many studies exceeds that of salmonella isolations from diarrheal stools of hospitalized patients. medical workers in many parts of the world confirmed that campylobacter jejuni was found more commonly in feces ...19846748977
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
rotaviruses in infants with diarrhea studied by viral rna electrophoresis in ankara, turkey.incidence of rotavirus in children under 2 years of age, admitted to 2 children's hospitals i n ankara, turkey, from july 1984-june 1985 was measured using viral rna electrophoresis in agarose gels. 375 children were selected at random from over 14,000 diarrhea admissions, and their stool specimens taken on admission were compared to those of 333 children without diarrhea. 61 (16.3%) of the diarrhea cases had detectable rotavirus, compared to 0% in controls. bacteriologic tests for salmonella ...19872851886
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