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resistance to tumor growth mediated by listeria monocytogenes. destruction of experimental malignant melanoma by lm-activated peritoneal and lymphoid cells.a murine experimental model of nonspecific tumor destruction mediated by cells activated by listeria monocytogenes (lm) is described. b16 melanoma growth is prevented or suppressed in the syngeneic host when tumor cells are inoculated in contact with viable lm. in vitro, cultured b16 cells are destroyed by lm immune peritoneal or splenic cells in the presence of the bacterial antigen(s). activation of lm immune cells in vitro is immunologically specific. replacement of lm by sheep red blood cell ...197656396
[influence of maternal antibodies on the primary and secondary immunoresponse of the juvenile organism (author's transl)].the immunization of mice with sheep erythrocytes served as model for the materno-fetal transfer of specific antibodies. it could be shown that passively transferred antibodies are able to induce a long-lasting suppression of the primary and secondary immuno-response in the offspring. this holds true both for active immunization before pregnancy and for passive immunization shortly before term. as it can be supposed that similar effects also apply to the human organism, this phenomenon must be co ...1979105206
effects of cannabinoids on host resistance to listeria monocytogenes and herpes simplex virus.previous investigations from our laboratories have demonstrated that cannabinoids possess immunosuppressive properties. the present studies were designed to determine whether these agents decrease host resistance to infections with listeria monocytogenes and herpes simplex virus type 2. host resistance was measured by changes in the 50% lethal dose of the pathogen in cannabinoid-treated and control mice. the effect of cannabinoids on resistance to l. monocytogens was dose dependent. delta-9-tetr ...1979313368
listeric septicaemia in sheep associated with tick-borne fever (ehrlichiosis ovis). 1977413348
listeria cell wall fraction: adjuvant activity in vivo and in vitro. 1975805002
[the rate of healthy carriers of listeria monocytogenes].the feces of 318 healthy adults were examined for l. monocytogenes by means of the cold enrichment and of two different selective media (nalidixic acid-trypaflavin-serum agar; braveny medium). 15 persons (4.7 percent) proved to be carriers. three of the 15 strains only caused hemolysis on sheep blood agar and belonged to serotypes 1/2b, 1/2c and 3c. the nonhemolytic strains without exception belonged to serotypes 4f and 4g. the braveny medium proved to be superior to the nalidixic acid-trypaflav ...1975805357
isolation of listeria monocytogenes type 04 from cases of keratoconjunctivitis in cattle and sheep.keratoconjunctivitis in cattle and sheep is usually recognized as a summer problem. the infective agency usually isolated from cattle is moraxella bovis. in sheep neisseria ovis and rickettsia (colesiota) conjunctivae are expected to be found. this report describes isolations of listeria monocytogenes from eye inflammations in housed cattle and sheep.1975806058
infections associated with ovine perinatal mortality in victoria.the occurrence of infections associated with perinatal mortality was investigated. in 1970, carcasses from 94 flocks distributed throughout victoria were examined by microbiological and histopathological techniques. carcasses from 4 of the same flocks were similarly examined in 1971. widespread occurrence of listeria monocytogenes and campylobacter fetus was demonstrated with l. monocytogenes being recognised in 25% of the flocks examined. no other bacterial pathogens commonly associated with ov ...1975809028
perinatal lamb mortality in western australia. 6. listeric infection.listeric abortion was diagnosed in sheep on 14 occasions between 1963-65, and 4 of these were associated with other major abortion pathogens. except for infection in one district, the outbreaks were scattered sporadically over the southern agricultural area. the diagnosis in 1963 was the first time listeriosis had been found in animals or man in western australia. l. monocytogenes was readily recovered in pure culture from all organs and sites cultured. all the strains were serotype 5 ivanov, wi ...1975809029
a direct plating method for monitoring the contamination of listeria monocytogenes in silage.twenty-two silage samples were analyzed for the presence of l. monocytogenes using five listeria selective plating media, with and without previous selective enrichment step. l. monocytogenes was recovered from 3 samples by both procedures, but direct plating allowed the quantification of listeria population. two of these positive samples were implicated in outbreaks of listeriosis in sheep; the l. monocytogenes population in these samples was about 10(6) cells/g. the l. monocytogenes population ...19921455943
hemolytic interactions of dermatophilus congolensis.the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ...19921621476
incidence of aeromonas and listeria spp. in red meat and milk samples in brisbane, australia.a total of 150 samples, 50 each of beef, lamb and pork from 10 local retail stores in brisbane metropolitan area as well as 150 raw bovine bulk milk tank samples obtained from queensland united foods (quf), were examined for the presence of aeromonas and listeria spp. over a period of 1 year. different sets of enrichment and plating media were used to recover the organisms with subsequent identification using conventional biochemical and serotyping techniques. a total of 509 isolates consisting ...19911904764
replica plating of colonies from listeria-selective agars to blood agar to improve the isolation of listeria monocytogenes from foods.bacterial colonies from listeria-selective agars were replica plated to sheep blood agar to screen for beta-hemolysis. by using the replica plating method to test for the beta-hemolytic characteristic of all the colonies growing on listeria-selective agars instead of picking 3 to 10 suspected colonies for further testing, we recovered listeria monocytogenes from 59 of 142 listeria-selective agar plates which contained colonies of hemolytic and nonhemolytic listeria species and were negative when ...19902117884
immunotoxicity of bis(tri-n-butyltin)oxide in the rat: effects on thymus-dependent immunity and on nonspecific resistance following long-term exposure in young versus aged rats.to investigate whether immune function suppression observed in an earlier study after short-term bis(tri-n-butyltin)oxide (tbto) exposure also occurred after long-term treatment, function studies for specific and nonspecific resistance were performed after exposure of weaned male rats to diets containing 0, 0.5, 5, or 50 mg tbto/kg for 4-6 and 15-17 months. treatment for 4.5 months had no effect on body weight but reduced thymus weight at 50 mg/kg. regarding the thymus-dependent immunity, delaye ...19902118283
influence of hydroxyethyl starch on humoral and cell-mediated immune responses in mice.the immunotoxicity of hydroxyethyl starch (hes), a reagent used in leukocytapheresis or as a plasma expander, was assessed. hes did not significantly alter host resistance to listeria monocytogenes or streptococcus pneumoniae. hes (4-32 ml/kg), as well as a physiological saline solution (32 ml/kg), did inhibit the in vitro lymphoproliferation of spleen cells from mice intravenously injected 1 hour prior to removal of the spleens; the proliferation induced by a t-cell and b-cell mitogen was suppr ...19852408361
comparison of lithium chloride-phenylethanol-moxalactam and modified vogel johnson agars for detection of listeria spp. in retail-level meats, poultry, and seafood.the effectiveness of modified vogel johnson agar and lithium chloride-phenylethanol-moxalactam agar for detection of listeria spp. in foods was compared by using the media to analyze retail-level meat, poultry, and seafood both by direct plating and in conjunction with a three-tube most-probable-number enrichment. the most-probable-number protocol detected listeria species, including listeria monocytogenes, in a substantial portion of the fresh meat and seafood samples. in most instances the lis ...19892494936
immunisation against infections caused by listeria monocytogenes in sheep.immunisation against listeriosis in sheep using a live, attenuated vaccine was introduced in norway in 1984. since then 65,000 to 80,000 animals have been vaccinated annually. information obtained by a questionnaire showed that the incidence of listeriosis decreased from approximately 4.0 per cent before the introduction of the vaccine to 1.5 per cent after vaccination started. the incidence of abortions was 0.7 per cent in vaccinated flocks compared to 1.1 per cent in unvaccinated flocks. there ...19892505430
the effect of t cell depletion from spleen cell allografts on graft-versus-host disease and long-term immune reconstitution in h-2 haplotype-identical murine combinations.graft-versus-host disease (gvhd) and states of immune reconstitution in allogeneic chimera mice across minor histocompatibility antigens were analyzed in excess of 9 months after injecting akr/jsea (akr) spleen cells into irradiated c3h/heslc (c3h) mice. when t cell-depleted akr spleen cells were used as inoculum cells, neither graft failure nor gvhd was seen for 9 months postgrafting in the c3h mice irradiated with 660 rad or more. in an akr - c3h (850 rad) model, thy1.1+ or l3t4+ t cell deplet ...19892515151
phospholipase c in listeria.the cooperative and antagonistic effect of extracellular bacterial proteins of streptococcus agalactiae, rhodococcus equi, corynebacterium ovis, and corynebacterium haemolyticum with proteins of listerial strains of various sources, species and serovars of the membrane of sheep erythrocytes was investigated. listeria monocytogenes and listeria ivanovii produce, beside listeriolysin, further proteins. their specific effect on the sheep erythrocyte membrane becomes apparent after the appearance of ...19892561039
suppression of immune response in the b6c3f1 mouse after dietary exposure to the fusarium mycotoxins deoxynivalenol (vomitoxin) and zearalenone.the effect that dietary exposure to the naturally-occurring fusarium graminearum toxins deoxynivalenol (don) and zearalenone (zea) may have on immune function was assessed in the b6c3f1 mouse. dietary don depressed the plaque-forming response to sheep red blood cells, the delayed hypersensitivity response to keyhole limpet haemocyanin and the ability to resist listeria monocytogenes. listerial resistance was similarly decreased in control mice fed restricted diets comparable to the dietary restr ...19872953660
immunomodulation and chlamydia: immunosuppression and the protective immune response to c. psittaci in mice.mice immunized intramuscularly with a low dose, viable inoculum of c. psittaci survived an otherwise lethal intraperitoneal challenge with the homologous chlamydial strain. immunized animals were not protected from intraperitoneal challenge by the unrelated pathogen, listeria monocytogenes. spleen cells from animals that exhibited protective immunity were suppressed in their proliferative responses to mitogens or chlamydial antigen in an in vitro blastogenic assay. this suppression was transfera ...19883059773
alteration of phage- and biotypes of listeria strains.ethyl-methyl-sulphonate mutants of listeria monocytogenes might be different from the parent strain in phage type and in splitting of lactose, maltose, melezitose, sucrose and trehalose. differences were observed in repeated control studies in phage type and carbohydrate-decomposition of 550 listeria strains isolated from a variety of sources (patients, healthy and dead animals). it has been concluded that certain carbohydrate tests are unsuitable for distinguishing biotypes of listeria. an impr ...19863092557
induction of immunotoxicity in mice by polyhalogenated biphenyls.acute administration of aroclor-1254 (500 mg/kg) or 3,4,5,3',4',5'-hexabromobiphenyl (hbb) (2-6 mg/kg) ip, profoundly inhibited the plaque forming response to subsequent challenge with sheep erythrocytes in ah locus positive (c57bl/6n or b6c3f1n) mice. these studies showed: the immunotoxicity results paralleled enzyme induction results insofar as hbb was approximately 100 times more potent than aroclor 1254; neither aroclor nor hbb treatment caused significant induction in the ah locus negative ...19863092783
[causes of lamb losses with special reference to listeriosis on a large-scale farm].epizootiological studies on lamb losses on a large scale farm (4.5 thousand mothers) were carried out. the results of the studies denied the direct effect of maize silage on the genesis of enzootia of listeriosis. the earlier reports were confirmed, i.e. that the direct cause of enzootia of listeriosis is the weakening of an animal organism due to other microbiological, zoohigienical, nutritional factors (e.g. not proper content of mineral compounds). maize silage has a destructive effect on the ...19863111517
abilities of human oligodendroglial cells and mouse schwann cells to phagocytose mycobacterium leprae and other mycobacteria.human oligodendroglial kg-1-c cells derived from human cerebral mixed glioma and mouse schwann cells derived from dorsal root ganglion were studied with respect to their abilities to phagocytose various mycobacteria, especially mycobacterium leprae, and other microorganisms. kg-1-c cells phagocytosed m. leprae at a markedly higher rate than balb/3t3, bhk 21, hela s3, mks-a tu-7, xc, tsv-5, n-18, and schwann cells but at a lower rate than peritoneal macrophages. schwann cells also exhibited subst ...19863510165
immunotoxicity studies in mice exposed to methyl isocyanate.the effects of methyl isocyanate (mic) on systemic immunity were evaluated in female b6c3f1 mice exposed via inhalation to 0, 1, or 3 ppm for 6 hr per day on four consecutive days. humoral immunity, measured as the antibody response to sheep erythrocytes, and natural killer cell activity were not affected by mic. furthermore, resistance to the infectious agents listeria monocytogenes, mouse malaria parasite, and influenza virus, or to b16f10 transplantable tumor cells, was not compromised by mic ...19863532429
modulation of cell-mediated resistance to listeriosis in mice given t-2 toxin.the modulating effect of preinoculation and postinoculation treatment with a single oral 4.0 mg/kg dosage of t-2 toxin on cell-mediated resistance was studied in mice inoculated with listeria monocytogenes. toxin treatment caused significant decreases in thymus and spleen weights, bone marrow cellularity, and in the total number of circulating leukocytes, lymphocytes, and neutrophils. enhancement or suppression of resistance to listeriosis was dependent on the time of toxin administration relati ...19873603564
the pathologic and immunologic effects of inhaled acrolein in rats.four groups of 40 male sprague-dawley rats each were exposed by inhalation to target concentrations of 0, 0.1, 1.0, and 3.0 ppm of acrolein 6 h/day, 5 days/week for 3 weeks. subsequent changes in local pulmonary immunity were determined by examining the number of antibody plaque-forming cells in the lung-associated lymph nodes following intratracheal immunization with sheep red blood cells. separate groups of rats were evaluated for blastogenic responsiveness to phytohemagglutinin-p and salmonel ...19873686549
immune protective mechanisms during pregnancy. i. cell-mediated immunity against listeria monocytogenes in pregnant mice.characteristics of protective mechanisms during pregnancy were investigated using neonatally thymectomized (ntx) and/or pregnant mice infected with sublethal doses of listeria monocytogenes, of which the explosive growth at an early phase of 2 or 3 days after infection is prevented by non-immune macrophages, and complete elimination at a late phase from 4 to 10 days after infection is attributed to the augmented functions of macrophages in co-operation with lymphokine-producing sensitized t lymp ...19863793136
vaccination against listeriosis in sheep. 19853931339
ovine listerial encephalitis: analysis, hypothesis and synthesis.analysis of field data suggested that listerial encephalitis in sheep was most common in winter and early spring in the age groups of sheep which would be cutting, changing and possibly losing teeth. it was hypothesised that under these conditions ingested listeria monocytogenes could reach the fine dental terminals of the trigeminal nerve causing an ascending neuritis and encephalitis. experimental inoculation of organisms into the dental pulp demonstrated the feasibility of this route of infec ...19854002594
survival versus growth of a facultative psychrotroph meat and products of meat. 19734200196
sequential studies of experimentally induced ovine listerial abortion: pathologic changes. 19744205017
proceedings: survival versus growth of a facultative psychrotroph. 19724206774
[listeria infections: some aspects of human and animal infection]. 19744207840
listeria monocytogenes isolations in alberta 1951-1970. 19724622759
infection of sheep with listeria monocytogenes. 19724630923
listeric abortion studies in sheep. iv. histopathologic comparison of natural and experimental infection. 19734634081
visceral and cerebral listeriosis in a lamb. 19664958036
role of lipids in the mechanism of sensitization of erythrocytes by bacterial antigens with polysaccharide determinants. 19674964833
an outbreak of abortion due to listeria monocytogenes in an experimental flock of sheep. 19674966927
[detection of listeria in bacteriological carcass inspection]. 19674969924
[further experimental studies on the pathogenesis of cerebral listeriosis in sheep. 3. the course of the experimental infection with l. monocytogenes in sheep with high protein and low protein nutrition]. 19674970714
[on the occurrence of listeriosis in germany]. 19684971816
[listeria monocytogenes. xi. complete results of a study of 3115 animal sera and 6319 human sera]. 19674981671
the serological activities of immunoconglutinin. 19694984463
[a study of serotypes of listeria monocytogenes isolated from various subjects]. 19694986310
[isolation of listeria monocytogenes from organs of young lambs].samples of organs from 50 lambs, 3-45 days old, brought to this laboratory from 44 different flocks for post mortem examination, were examined for listeria monocytogenes. various diseases were found as cause of the death. the bacterium was isolated from 3 lambs (6%) which had died from e. coli-septicaemia. two of these lambs were 3 days old whereas the third was 23 days.19836424092
[occurrence of listeria , bedsonia, leptospira and brucella antibodies in workers on animal husbandry farms in wielkopolska]. 19846429736
[enzootic septicemic listeriosis in a migrating flock of sheep]. 19846438797
listeria monocytogenes infections in canada.since its first isolation by murray in 1926 listeria monocytogenes has become recognized as a significant pathogen occurring worldwide and involving a wide range of wild and domestic animals including man. the first confirmed human listeriosis case in canada was published by stoot in 1951. a later survey based on records maintained at the laboratory centre for disease control, ottawa showed 101 cases detected over a 21 year period in nine provinces. the overall mortality was 30%. the most freque ...19846442654
depression by pseudomonas aeruginosa of two t-cell-mediated responses, anti-listeria immunity and delayed-type hypersensitivity to sheep erythrocytes.pseudomonas aeruginosa was studied for its effects on t-cell-mediated responses in mice, as exemplified by anti-listeria immunity and delayed-type hypersensitivity to sheep erythrocytes. immunity to listeria monocytogenes was measured by quantitation of bacteria in spleens and mortality; delayed hypersensitivity to sheep erythrocytes was tested by the footpad reaction. three different preparations of p. aeruginosa were used: the supernatant of a heat-killed culture, living bacteria, and heat-kil ...19826461605
toxicity of bis(tri-n-butyltin)oxide in the rat. ii. suppression of thymus-dependent immune responses and of parameters of nonspecific resistance after short-term exposure.to evaluate the functional significance of bis(tri-n-butyltin)oxide (tbto)-induced thymus atrophy, lymphocyte depletion in spleen and lymph nodes, lymphopenia, and increased serum igm and decreased igg concentrations, in vivo and in vitro function studies were performed for specific and nonspecific resistance. weaned male rats were fed diets containing 0, 20, or 80 mg tbto/kg for at least 6 weeks. regarding the thymus-dependent immunity, delayed-type hypersensitivity reactions to ovalbumin as we ...19846474470
primary immune response of mice to sheep erythrocytes during the course of infection with listeria monocytogenes.the influence of an infection with a sublethal dose of viable listeria monocytogenes on the primary antibody-forming potential of mice against sheep erythrocytes (se) was monitored by means of counting plaque-forming cells (pfc) in the spleen and measuring circulating antibodies against se. additionally, the course of infection was followed by determination of viable bacteria in the spleen. as against the injection of killed l. monocytogenes, infection with viable bacteria did not display any si ...19806775440
mechanism of cellular suppression induced by oral tilorone treatment of mice.specific-pathogen-free b6d2 f1 hybrid mice were treated orally with tilorone hydrochloride (100 mg/kg of body weight per day) and infected with sublethal doses of listeria monocytogenes, salmonella enteritidis, mycobacterium bovis (bcg pasteur), or m. tuberculosis erdman. daily tilorone treatment inhibited the cell-mediated response to all of the intracellular parasites, and most of the mice succumbed to the challenge. tilorone suppressed delayed hypersensitivity responses to the microbial sensi ...19806777302
synergistic hemolysis phenomenon of listeria monocytogenes and corynebacterium equi. 19806785505
development of a simple passive haemagglutination-inhibition assay for listeria monocytogenes lipoteichoic acid.a simple assay for listeria lipoteichoic acid was devised. it is based on the property of this substance to attach spontaneously to erythrocytes. thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. cross-reactivity of lipoteichoic acid from lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal ...19816792287
ontogeny of murine macrophages: functions related to antigen presentation.macrophage function in neonates was dissected into four components: antigen uptake and catabolism, cytotoxicity, antigen presentation, and the production of the lymphostimulatory molecule interleukin-1 (also called thymocyte mitogenic protein or lymphocyte-activating factor). the uptake and catabolism of 125i-labeled listeria monocytogenes was equivalent in macrophages from adult and neonatal mice. however, interactions between macrophages from neonates, heat-killed listeria organisms, and immun ...19826804386
[special position of strongly haemolytic strains of the genus listeria].the special status of the strongly haemolytic strains of serovar (sv) 5 of listeria monocytogenes is reviewed. besides some biochemical reactions sv 5 strains are characterized by their excessive haemolysis on sheep blood agar and by a positive result of the camp test with r. equi. the haemolysis is caused by a soluble thermolabile protein which stimulates the formation of antibodies and which is inhibited by cholesterol. sv 5-strains are less virulent than strains of sv 1/2a and sv 4b. the anti ...19826812318
different haemolytic activities of listeria monocytogenes strains determined on erythrocytes of various sources and exploiting the synergism of equi-factor. 19826817557
immunosuppression, nonspecific b-cell activation, and mitogenic activity associated with a high molecular weight component from listeria monocytogenes.a high molecular component of a saline extract derived from listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. the same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. the material was mitogenic to b but not to t lymphocytes in vitro. responses to sheep and horse erythrocytes as well as to lipopolysaccharide were suppressed. immunosuppression was dose depende ...19826820330
host defenses in murine malaria: analysis of plasmodial infection-caused defects in macrophage microbicidal capacities.macrophage-dependent killing of facultative intracellular bacteria was markedly impaired by overt erythrocytic plasmodium yoelii or plasmodium berghei infection of mice. p. yoelii infection was capable of ablating not only the macrophage microbicidal capacity of "normal" animals but also the bactericidal capacities of "activated" macrophages. the uptake by spleen and liver of an intravenous challenge of listeria monocytogenes was not altered by plasmodial infection, but within hours of injection ...19817012000
rapid diagnosis of listeria monocytogenes by immunohistochemistry in formalin-fixed brain tissue. 19957771062
effect of heat treatment on listeria monocytogenes and gram-negative bacteria in sheep, cow and goat milks.sheep milk, compared with cow and goat milk, had a protective effect on gram-negative bacteria and listeria spp. heated at 65 degrees c in a test-tube method. this effect was not solely due to fat content as cow milk artificially reconstituted to 10% homologous fat was not as protective. listeria monocytogenes in whole sheep, cow and goat milks at an inoculum level of 1 x 10(6) cfu ml-1 was heated at 68 degrees c for 15 s in the plate pasteurizer and survival was only detected in whole sheep mil ...19938226390
serotyping and distribution of listeria isolates from cases of ovine listeriosis. 19938236705
listeria monocytogenes phosphatidylinositol (pi)-specific phospholipase c has low activity on glycosyl-pi-anchored proteins.the ability of the phosphatidylinositol-specific phospholipase c (pi-plc) from listeria monocytogenes to hydrolyze glycosyl phosphatidylinositol (gpi)-anchored membrane proteins was compared with the ability of the pi-plc from bacillus thuringiensis to hydrolyze such proteins. the l. monocytogenes enzyme produced no detectable release of acetylcholinesterase from bovine, sheep, and human erythrocytes. the cleavage of the gpi anchors of alkaline phosphatase from rat and rabbit kidney slices was l ...19938253689
membrane damage and interleukin-1 production in murine macrophages exposed to listeriolysin o.to obtain some insight into the interaction between listeriolysin o (llo) and the macrophage membrane, we examined the effect of purified listeria monocytogenes hemolysin on the viability and functions of mouse peritoneal exudate macrophages. the study showed that purified llo impaired a variety of functions of the macrophages. first, it suppressed the luminol-dependent chemiluminescence response of macrophages. second, it suppressed the phagocytic ingestion of opsonized sheep erythrocytes and l ...19938454336
novel mechanism for the camp reaction between listeria monocytogenes and corynebacterium equi.the synergistic hemolytic reaction between listeria monocytogenes, staphylococcus aureus and corynebacterium equi on blood agar plates was explored using a modified test-tube assay. a synergistic lysis of sheep red blood cells was observed with l. monocytogenes and either s. aureus or c. equi, using crude extracts. synergism was also found with l. monocytogenes and purified cholesterol oxidase from pseudomonas fluorescens and brevibacterium brevi. crude extracts of s. aureus sensitized sheep red ...19938466816
the use of listeriolysin o in an elisa, a skin test and a lymphocyte blastogenesis assay on sheep experimentally infected with listeria monocytogenes, listeria ivanovii or listeria innocua.purified listeriolysin o (llo) was evaluated as a specific antigen to detect both humoral and cell mediated immune responses of sheep infected with listeria monocytogenes. six sheep (two in each group) were orally inoculated with 10(10) organisms of l. monocytogenes, l. ivanovii, or l. innocua. only the l. monocytogenes inoculated sheep had an elevated temperature (> 42 degrees c) and after 15 days had anti-llo antibodies as assessed by an elisa. in a blastogenesis assay, only peripheral blood m ...19968828131
serological diagnosis of bovine, caprine, and ovine mastitis caused by listeria monocytogenes by using an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay for detecting listeria monocytogenes antibodies in bovine (n = 35), caprine (n = 27), and ovine (n = 30) milk samples was evaluated by comparison with bacteriological examination. microtiter plates were coated with proteins obtained from culture supernatant, and antibodies were revealed with a monoclonal antibody able to react with the immunoglobulins belonging to the three animal species. the arithmetic mean optical density (od) of milk samples infected with ...19979163495
effects of fumonisin b1 on the immune system of sprague-dawley rats following a 14-day oral (gavage) exposure.the effects of fumonisin b1 (fb1) on the immune system of sprague-dawley rats were investigated. groups of male and female rats (10 rats/group) were gavaged daily for 14 days with doses of 0, 5, 15, and 25 mg/kg body wt/day and the primary (igm) response to sheep red blood cells expressed as plaque-forming cell numbers/10(6) spleen mononuclear leukocytes (pfc/10(6) splenocytes) and pfc/spleen was determined. there was a significant dose-related linear trend toward decreased pfc/10(6) splenocytes ...19979325027
[studies on the occurrence of listeria monocytogenes in fecal samples of domestic and companion animals].listeria (l.) monocytogenes was isolated from 33.3% fecal samples of 138 cattle (29 strains serovar 1/2b, 10 strains serovar 1/2a, 7 strains serovar 4ab), from 8% fecal samples of 100 hens (5 strains serovar 1/2b, 1 strain serovar 1/2a, 4ab and 4b each), from 8% fecal samples of 100 sheep (6 strains serovar 1/2a, 1 strain serovar 1/2b and 4ab each), from 5.9% fecal samples of 34 pigs (2 strains serovar 4ab), from 4.8% fecal samples of 400 horses (7 strains serovar 1/2a, 6 strains serovar 1/2b an ...19959376045
listeriosis.listeria monocytogenes is ubiquitous in nature and is part of the normal flora of the distal portion of the intestinal tract of numerous animal species. listeriosis is an emerging food borne disease that is responsible for approximately 1,700 cases of human illness each year and 650 deaths. listeria is the cause of three main disease entities in animals and humans: neural, visceral, and reproductive. clinical signs associated with the three forms are discussed along with diagnosis, therapy, prev ...19989532671
the effects of inoculation of listeria monocytogenes into the ovine mammary gland.in each of two experiments, the effects of inoculation of listeria monocytogenes into the ovine mammary gland were studied. in the first experiment, ewes were challenged with one or other of five different listeria spp. isolates to study differences in their pathogenicity. in the second, ewes were challenged with l. monocytogenes serotype 1/2a to study the sequential features of the infection. the reaction of the mammary glands was assessed by bacteriological, cytological and histological method ...19989549859
enhanced detection and enumeration of listeria monocytogenes from foodstuffs and food-processing environments.listeria monocytogenes blood agar (lmba) was compared to listeria selective agar based on lithium chloride and ceftazidime (la) and to the oxford and palcam media recommended by iso and idf for the detection and enumeration of l. monocytogenes from foodstuffs and food-processing environments. lmba is based on trypticase soy agar with the following additions: sheep blood (5%) and as selective agents lithium chloride (10 g/l), polymyxin b sulphate (10 mg/l) and ceftazidime (20 mg/l), whereas the s ...19989600613
listeriolysin o: cholesterol inhibits cytolysis but not binding to cellular membranes.listeriolysin o (llo) binds to cholesterol-containing membranes in which it oligomerizes to form pores. preincubation of the toxin with cholesterol is known to inhibit haemolysis, whereas the oxidized form of cholesterol has no inhibitory effect. using immunoblot analyses and flow cytometry we demonstrate that preincubation with cholesterol does not influence binding of the listeriolysin-cholesterol complex to red blood cells, eukaryotic cells or artificial membranes. lytic activity of membrane- ...19989680200
in vitro and in vivo macrophage function can occur independently of slp-76.expression of sh2 domain-containing leukocyte-specific phosphoprotein of 76 kda (slp-76), a hematopoietic cell-specific adapter protein, is required to couple syk family tyrosine kinase activation to downstream mediators such as phospholipase c (plc)-gamma following tcr, platelet collagen receptor and mast cell fc epsilon r stimulation. in addition to t cells, mast cells and platelets, slp-76 is expressed in monocytes and macrophages. to determine the role of slp-76 in fc gamma r-stimulated sign ...200010837416
the impact of the quality of silage on animal health and food safety: a review.this paper reviews the microbiological aspects of forage preserved by ensilage. the main principles of preservation by ensilage are a rapid achievement of a low ph by lactic acid fermentation and the maintenance of anaerobic conditions. the silage microflora consists of beneficial micro-organisms, i.e. the lactic acid bacteria responsible for the silage fermentation process, and a number of harmful micro-organisms that are involved in anaerobic or aerobic spoilage processes. micro-organisms that ...200011087133
comparison of the efficacy of different techniques, culture media, and sources of blood in determining the hemolytic activity of listeria spp..hemolytic activity is a fundamental criterion for the differentiation of listeria species; therefore, a simple and inexpensive procedure to clearly distinguish hemolytic strains from each other and from nonhemolytic strains would be of great aid. we compared the efficacy of several techniques, culture media, and types of blood in demonstrating the hemolysis of listeria spp. the hemolytic activities of listeria monocytogenes and listeria seeligeri were more easily detected with a red blood cell t ...200111547885
prevalence of listeria spp. in feces and carcasses at a lamb packing plant in brazil.the objective of this work was to study the occurrence of listeria species in feces and on dressed and cooled carcasses of lambs at a packing plant in brazil. listeria spp. were recovered on oxford and palcam agars. the 35 fecal samples yielded listeria welshimeri (20%) and listeria innocua (8.6%). the 69 carcass samples yielded l. innocua (34.8%), listeria monocytogenes (4.3%), and listeria ivanovii (1.5%). more listeria spp. were recovered with two selective agars than with either agar alone.200312597497
meconium enhances the growth of perinatal bacterial pathogens.to demonstrate the effects of meconium on growth of bacterial pathogens, which are common causes of intra-amniotic infection and neonatal sepsis.200312634904
methods for isolation of listeria monocytogenes from sheep. 195313031013
isolation of listeria monocytogenes from oat silage.a culture of listeria monocytogenes isolated from the brain of an infected sheep from a flock fed contaminated silage and cultures isolated from this silage were all serological type 4b, establishing a definite epidemiological relationship.196013708072
failure of animal passage to increase the virulence of listeria monocytogenes.silverman, sidney j. (u.s. army biological laboratories, fort detrick, frederick, md.), james f. drawdy, and donald a. kautter. failure of animal passage to increase the virulence of listeria monocytogenes. j. bacteriol. 86:92-94. 1963.-three strains of listeria monocytogenes, jhh, a4413, and 53p380, were serially passed through mice, guinea pigs, or monkeys via the intracerebral, intraperitoneal, and intraspinal routes, respectively. the first two strains had been maintained on laboratory mediu ...196314051827
macrophage activation and fcgamma receptor-mediated signaling do not require expression of the slp-76 and slp-65 adaptors.the src-homology 2 domain-containing, leukocyte-specific phosphoprotein of 76 kda (slp-76) is a hematopoietic adaptor that plays a central role during immunoreceptor-mediated activation of t lymphocytes and mast cells and collagen receptor-induced activation of platelets. despite similar levels of expression in macrophages, slp-76 is not required for fc receptor for immunoglobulin g (igg; fcgammar)-mediated activation. we hypothesized that the related adaptor slp-65, which is also expressed in m ...200414694181
clinical and histopathological aspects of naturally occurring mastitis caused by listeria monocytogenes in cattle and ewes.listeria monocytogenes was isolated from the milk of two cows and two sheep with mastitis in one quarter and one udder half. the animals were observed over a period of 2-12 months. clinical examination of the udder, bacteriological examinations and determination of somatic cell counts of milk samples were performed monthly. all four cases suffered from a subclinical mastitis characterized by an elevated somatic cell count (0.8-10.1 x 10(6) cells/ml), a persistent shedding of listeria and by a no ...200415228552
a combination of diagnostic tools for rapid screening of ovine listeriosis.a combined serological and pcr method for the detection of listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. seventy-eight milk samples and 157 serum samples were analysed using a l. monocytogenes pcr detection kit and an anti-listeriolysin o igg immunoassay kit. the combined use of these commercial kits allowed a rapid and effective detection of l. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after ...200616487553
dependence of the lethal effect of pore-forming haemolysins of gram-positive bacteria on cytolytic activity.among bacterial haemolysins, cholesterol-dependent cytolysins (cdcs) produced by various gram-positive bacteria are known to exhibit a lethal activity in mice. in this study, recombinant cdcs of streptolysin o, pneumolysin, ivanolysin o, listeriolysin o and several listeriolysin o mutants were constructed and the relationship between cytolytic activity and the lethal activity of each recombinant protein in mice was examined. specific activity for cytolysis was determined by a quantitative haemol ...200616585635
rapid determination of multi-locus sequence types of listeria monocytogenes by microtemperature-gradient gel electrophoresis.this report presents a new method for identifying multi-locus sequence types of listeria monocytogenes by microtemperature-gradient gel electrophoresis (mu-tgge). genomic comparison of l. monocytogenes serovar 1/2a strains egd-e and f6854 allowed selection of novel polymorphic sequences lmo0386 and lmo0428 as optimum regions for mu-tgge analysis, in addition to the previously identified lmo0297 gene. sequence analysis of a total of 48 standard strains revealed that the strains could be grouped i ...200717628727
adaptation and cross-adaptation of listeria monocytogenes and salmonella enterica to poultry decontaminants.information on the potential for acquired reduced susceptibility of bacteria to poultry decontaminants occurring is lacking. minimal inhibitory concentrations (mics) were established for assessing the initial susceptibility and the adaptative and cross-adaptative responses of four bacterial strains (listeria monocytogenes serovar l/2a, l. monocytogenes serovar 4b, salmonella enterica serotype typhimurium, and s. enterica serotype enteritidis) to four poultry decontaminants (trisodium phosphate, ...200919412596
neuropathogenesis of naturally occurring encephalitis caused by listeria monocytogenes in ruminants.listeriosis is a serious food-borne disease with increasing frequency in humans and ruminants. despite the facts that in both hosts, listeriosis can occur as rhombencephalitis and ruminants are a reservoir of listeria monocytogenes (lm) strains pathogenic for humans, little work has been done on the pathogenesis in ruminants. this study investigates the neuropathogenesis of listeric encephalitis in over 200 natural cases in cattle, sheep and goats by analyzing anatomical distribution, severity, ...201019476464
diagnosis of placental pathogens in small ruminants by immunohistochemistry and pcr on paraffin-embedded samples.a histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for pcr detection of chlamydophila abortus, coxiella burnetii, salmonella abortusovis, brucella melitensis, listeria monocytogenes and toxoplasma gondii. most of the cotyledons showed different degrees of placenti ...200919666916
inhibition by lactobacillus sakei of other species in the flora of vacuum packaged raw meats during prolonged storage.the abilities of five lactobacillus sakei strains and one lactococcus lactis strain to retain inhibitory activity against several target organisms in the flora of product during 12 weeks storage of vacuum-packaged lamb and beef was investigated. l. sakei strains were generally found capable of developing dominant populations on both beef and lamb. l. lactis 75 grew poorly on lamb did not inhibit co-inoculated brochothrix thermosphacta. lamb inoculated with the sakacin-a producer l. sakei lb706 h ...200919835775
production, characterization, and antimicrobial activity of a bacteriocin from newly isolated enterococcus faecium ij-31.this work aimed to isolate and characterize enterococcus spp. from indigenous dairy products in islamabad, pakistan. by classical microbiological techniques, one strain from a butter sample was identified to be enterococcus faecium, and we designated it e. faecium ij-31. the precise identity of this strain was then established by determining the sequence of its 16s and 23s rrna genes. the sequence homology searches revealed matches with a number of previously reported strains, such as e. faecium ...201020051203
the lectin-like domain of tnf protects from listeriolysin-induced hyperpermeability in human pulmonary microvascular endothelial cells - a crucial role for protein kinase c-alpha inhibition.listeriosis can lead to potentially lethal pulmonary complications in newborns and immune compromised patients, characterized by extensive permeability edema. listeriolysin (llo), the main virulence factor of listeria monocytogenes, induces a dose-dependent hyperpermeability in monolayers of human lung microvascular endothelial cells in vitro. the permeability increasing activity of llo, which is accompanied by an increased reactive oxygen species (ros) generation, rhoa activation and myosin lig ...201020074664
smcl as a novel diagnostic marker for quantitative detection of listeria ivanovii in biological samples.to develop a novel molecular tool for the quantitative detection of the ruminant pathogen listeria ivanovii in different biological matrices.201020337766
matrix metalloproteinase expression in sheep with listerial meningoencephalitis.matrix metalloproteinases (mmps) have been implicated in the pathogenesis of several central nervous system (cns) diseases. in this study, we investigated the presence of listeria monocytogenes antigens and detected the expression of mmp-9 and mmp-7 in the brains of 22 sheep with clinical signs and histopathological findings characteristic of listerial meningoencephalitis. archived sections from the brainstem, cerebrum, and cerebellum were stained for immunohistochemistry. l. monocytogenes antig ...201121345473
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