the measurement of three cytokine transcripts in naïve and sensitized ovine peripheral blood mononuclear cells following in vitro stimulation with bluetongue virus serotype-23.bluetongue (bt), a serious disease of sheep and some wild ruminants, is caused by bluetongue virus (btv), a member of the family, reoviridae. the current research thrust for controlling bt is on development of efficient vaccines, necessitating clear understanding of ovine immunology. at present, comparative studies on cytokine gene expression profiles of naïve and btv-sensitized peripheral blood mononuclear cells (pbmc) in sheep have not been clearly understood. in the present study, pbmc from n ...201021349379
apoptosis and immuno-suppression in sheep infected with bluetongue virus serotype-23.the role of apoptosis in pathogenesis of bluetongue (bt) has been suggested from various in vitro studies. however, to date, no clear data are available regarding btv-induced apoptosis and its consequences in natural host, sheep. in the present study, bluetongue virus (btv)-induced apoptosis was studied in sheep blood and splenic mononuclear cells by analyzing annexin(+)-propidium iodide(-) early apoptotic cells, dna ladder pattern, and caspase-3 gene expression. the onset of apoptosis and lymph ...201020347236
the effects of vaccination of merino ewes with an attenuated australian bluetongue virus serotype 23 at different stages of gestation.a cell culture attenuated australian bluetongue virus serotype 23 (blu23) prototype vaccine was assessed for its effects on pregnant merino sheep. seventy-six ewes were vaccinated at 5 different stages of gestation, and the failure to lamb at term was as follows: 35 to 43 days of gestation, 20/36 (56%); 57 to 64 days of gestation, 3/10 (30%); 81 to 88 days of gestation, 3/10 (30%); 109 to 116 days of gestation, 0/10 (0%); 130 to 137 days of gestation, 0/10 (0%). of 30 ewes vaccinated with a cell ...19958825310
characterization of an indian bluetongue virus isolate by rt-pcr and restriction enzyme analysis of the vp-7 gene sequence.the reverse transcription-polymerase chain reaction (rt-pcr) was standardized to amplify the vp-7 gene sequences of an indian isolate of bluetongue virus serotype 23. using two different sets of primers, a sequence of 1156 bp comprising the complete coding sequence of the vp-7 gene and its 770 bp internal sequence were amplified. the sensitivity of rt-pcr, using these two sets of primers individually was 40 pg and 4 pg, with the external and internal primers, respectively, whereas the nested pcr ...200011014609
bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction.eight sheep vaccinated with 10(6) pfu of attenuated australian bluetongue virus serotype 23 (btv 23a) and eight btv-free sheep were challenged with virulent btv 23. there was little subsequent variation in the mean clinical score, or in the mean lymphocyte and platelet concentrations in the peripheral blood of the eight vaccinated sheep. there was a marked thrombocytopenia and lymphopenia in the naive sheep as the mean lymphocyte and platelet concentrations fell to a minimum at days 8 and 11 aft ...19948048910
a comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses.the pathogenesis of bluetongue (bt) could vary with route of inoculation. using laboratory-passaged moderately virulent bluetongue virus serotype 23 (btv-23), one of the most prevalent indian serotype, we investigated the pathogenesis of bt in intradermally (id) and intravenously (iv) inoculated native sheep. the id inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to iv inoculation. btv-23 detection by real-time rt-pcr and isolation studies revea ...201121511346
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