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identification of rotavirus vp6-specific cd4+ t cell epitopes in a g1p[8] human rotavirus-infected rhesus macaque.a non-human primate model was used to evaluate its potential for identification of rotavirus viral protein 6 (vp6) cd4+ t cell epitopes. four juvenile rhesus macaques were inoculated with a mixed inoculum (g1p[8] and g9p[8]) of human rotaviruses. infection accompanied by g1p[8] shedding was achieved in the two macaques that had no rotavirus immunoglobulin a (iga) in plasma. to measure the interferon gamma (ifn-gamma) and tumor necrosis factor (tnf) anti-viral cytokines produced by peripheral cd4 ...200820401320
a rotavirus strain isolated from pig-tailed macaque (macaca nemestrina) with diarrhea bears a p6[1]:g8 specificity.a distinct rotavirus strain (ptrv) was isolated in cell cultures from a stool sample obtained from a diarrheic 3-year-old female pig-tailed macaque (macaca nemestrina) that was born at the breeding colony of the university of washington in seattle. unlike other known simian rotavirus strains including vervet monkey rotavirus sa11 which bears p5b[2]:g3 or p6[1]:g3 specificity, rhesus monkey rotavirus mmu18006 with p5b[3]:g3 specificity, pig-tailed macaque rotavirus yk-1 with p[3]:g3 specificity a ...200616242747
defining t-cell-mediated immune responses in rotavirus-infected juvenile rhesus macaques.the appearance of virus-specific cd4(+) and/or cd8(+) t lymphocytes in peripheral blood of captive juvenile rhesus macaques (macaca mulatta) was observed following rotavirus infection. these cell-mediated immune responses were measured following experimental or natural infection after rotavirus was isolated from stool specimens of asymptomatic animals. the virus isolated was a new strain of simian rotavirus that we named tuch (for tulane university and cincinnati children's hospital). restimulat ...200415367591
serotypic characterization of outer capsid spike protein vp4 of vervet monkey rotavirus sa11 strain.the vervet monkey rotavirus sa11, a prototype strain of group a rotaviruses, has been shown to possess vp7 serotype 3 specificity but its neutralization specificity with regard to the other outer capsid protein vp4 has not been elucidated. we thus determined its vp4 specificity by two-way cross-neutralization with guinea pig antiserum prepared with a single gene substitution reassortant that had only the vp4-encoding gene from the simian rotavirus sa11 strain and remaining ten genes from human r ...19989687880
[immunofluorescence reaction for rotavirus analysis in fecal specimens].testing for rotavirus detection in faeces from infants with acute diarrhea can be carried out, with high sensitivity, by immunofluorescence on rhesus monkey kidney cell lines (ma-104). preincubation of viral inoculum with trypsin enhances further the sensitivity. in the present study we tried to assess the optimal conditions for detection of viral antigens, by analyzing the expression of virus-specific proteins at different times of infection and the effects of trypsin presence during virus adso ...19836330786
investigation of genetic interaction between simian rotavirus sa11 and reovirus.rotavirus and reovirus are physically and genetically very similar. their genetic interaction would have important implications for understanding both the epidemiology of rotavirus and the biological basis of rotavirus pathogenicity. a permissive line of rhesus macaque kidney cells, ma104, was infected with the simian rotavirus sa11 and various temperature sensitive mutants of reovirus (type 3 groups a, b, c, d, g, type 1 m79, and type 2 122). the mixed cell infections were carried out at permis ...19826289051
susceptibility of nonhuman primate species to infection by simian rotavirus sa-11.enzyme-linked immunosorbent assay examination of sera from three nonhuman primate species demonstrated the presence of antibody reacting with simian rotavirus sa-11 and calf rotavirus c486. the occurrence of this antibody in sera from adult, wild-caught animals suggests natural rotovirus infection. the occurrence of antibody was highest in the chimpanzee and declined, respectively, in the rhesus macaque and in the squirrel monkey. inoculation of three infant rhesus macaques, a nursery-reared chi ...19806254409
the in vitro antiviral activity of isoprinosine on simian rotavirus (sa-11).1. the antiviral effect of isoprinosine on simian rotavirus (sa-11) replication was studied using ma-104 cell cultures from rhesus monkey fetal kidney. 2. isoprinosine (n,n-dimethylamino-2-propanol-p-acetamidobenzoate in association with inosine) added after viral infection (therapeutic test) inhibited viral replication by more than 90%. in these experiments, the drug was added to the medium and replaced daily at concentrations varying from 62.5 micrograms/ml to 1 mg/ml. viral inhibition activit ...19892483980
monoclonal antipeptide antibodies recognize epitopes upon vp4 and vp7 of simian rotavirus sa11 in infected ma104 cells.to study morphogenetic events of rotavirus sa11-infected ma104 cells with strictly defined reagents we produced monoclonal antibodies against synthetic peptides from both outer capsid proteins vp4 (aa residues 228-241: qntrnivpvsivsr) and vp7 (aa residues 319-326: saafyyrv) of simian rotavirus sa11. two of the selected monoclonal antibodies proved to be reactive with determinants of sa11-infected ma104 rhesus monkey kidney cells, with purified sa11 as well as with the particular peptides used fo ...19921370605
isolation and characterization of an equine rotavirus.a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ...19836313746
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