Publications

TitleAbstractYear
Filter
PMID
Filter
typing of clinical mycobacterium avium complex strains cultured during a 2-year period in denmark by using is1245.in the present study restriction fragment length polymorphism analyses with the recently described insertion sequence is1245 as a probe was performed with clinical mycobacterium avium complex strains cultured in denmark during a 2-year period. the overall aim of the study was to disclose potential routes of transmission of these microorganisms. as a first step, the genetic diversity among isolates from aids patients and non-human immunodeficiency virus (hiv)-infected patients was described. in a ...19999986819
comparative macrorestriction and rflp analysis of mycobacterium avium subsp. avium and mycobacterium avium subsp. hominissuis isolates from man, pig, and cattle.in germany, tuberculous lesions in slaughtered pigs due to infection with members of the mycobacterium avium complex are increasingly reported. contaminated food originating from pig or other livestock is discussed as potential source of human infection. m. avium isolates from man (n=45), pig (n=29), and cattle (n=13) were characterised by restriction fragment length polymorphism (rflp) with respect to insertion sequences is1245 and is901 as well as by xbai-based pulsed-field gel electrophoresis ...200616797885
granulomatous lesions in lymph nodes of slaughter pigs bacteriologically negative for mycobacterium avium subsp. avium and positive for rhodococcus equi.the prevalence of granulomatous lesions in lymph nodes of pigs was studied. from january till august 2004 in two slaughterhouses in the netherlands 2,116,536 pigs were examined for the presence of granulomatous lesions in the sub-maxillary lymph nodes. in 15,900 (0.75%) of these pigs, lesions could be detected. nine farms with the highest incidence of lesions were selected for a more detailed pathological and bacteriological examination. on these farms, the prevalence of lesions in sub-maxillary ...200717126501
a standardised restriction fragment length polymorphism (rflp) method for typing mycobacterium avium isolates links is901 with virulence for birds.a standardised method for pvuii-psti-is901 restriction fragment length polymorphism (rflp) typing was developed and evaluated against 173 isolates of mycobacterium avium subsp. avium and m. avium subsp. silvaticum originating from birds (n=46) and their aviaries (n=5), pigs (n=85), cattle (n=18), reference serotype strains (n=9), humans (n=7), a horse (n=1), a nutria (n=1), and strain m. avium subsp. avium st 18 (formerly m. avium subsp. paratuberculosis st 18). pvuii-is1245 rflp typing was also ...200314499991
experimental infection of eurasian wild boar with mycobacterium avium subsp. avium.the eurasian wild boar (sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. we aimed to characterize the first experimental mycobacterium avium subsp. avium (maa) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. twelve 1-4-month-old wild boar piglets were housed in class iii bio-containment facilities. four concentrations of maa suspension were used: 10, 10(2) and 10(4) mycobacteria (2 animals each ...201020116184
cloning and expression of the haloalkane dehalogenase gene dhma from mycobacterium avium n85 and preliminary characterization of dhma.haloalkane dehalogenases are microbial enzymes that catalyze cleavage of the carbon-halogen bond by a hydrolytic mechanism. until recently, these enzymes have been isolated only from bacteria living in contaminated environments. in this report we describe cloning of the dehalogenase gene dhma from mycobacterium avium subsp. avium n85 isolated from swine mesenteric lymph nodes. the dhma gene has a g+c content of 68.21% and codes for a polypeptide that is 301 amino acids long and has a calculated ...200212147465
new probes used for is1245 and is1311 restriction fragment length polymorphism of mycobacterium avium subsp. avium and mycobacterium avium subsp. hominissuis isolates of human and animal origin in norway.mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. some m. avium subspecies are opportunistic pathogens for animals and humans. they are ubiquitous in nature and can be isolated from natural sources of water, soil, plants and bedding material. isolates of m. avium originating from humans (n = 37), pigs (n = 51) and wild birds (n = 10) in norway were examined by is1245 and is1311 rflp using new and spe ...200717335590
molecular evidence to support a proposal to reserve the designation mycobacterium avium subsp. avium for bird-type isolates and 'm. avium subsp. hominissuis' for the human/porcine type of m. avium.in an attempt to clarify the taxonomy of the mycobacterium avium complex, the relationship between is1245 rflp, growth temperature, 16s rdna signature sequences and the 16s-23s rdna internally transcribed spacer (its) of 160 m. avium-complex isolates from different sources was investigated. all 70 isolates identified as m. avium by inno-lipa mycobacteria (innogenetics, belgium), a dna probe test that targets the its, and by 16s rdna analysis carried multiple copies of is1245. three isolates with ...200212361252
distribution of mycobacterium avium subsp. avium and m. a. hominissuis in artificially infected pigs studied by culture and is901 and is1245 quantitative real time pcr.mycobacterium avium subsp. avium (maa) and m. a. hominissuis (mah) belong to the mycobacterium avium complex (mac) and are frequently associated with diseases in animals and humans. the aim of this study was to develop a system for rapid and accurate real time quantitative pcr (qpcr) identification and quantification of maa and mah. this study included 22 per os infected pigs, of which 10 were infected with maa, 10 with mah and 2 were present as a negative control group. from each animal, 21 dif ...201020227841
[avian mycobacteriosis in humans remains a threat in the czech republic].the members of mycobacterium avium complex belong to the most common agents causing mycobacterioses in humans. they are classified as a potentially patogenic mycobacteria, although mycobacterium avium subsp. avium represent veterinary and economic risks in birds (mainly poultry) as well as mammals (pigs etc.). infected animals and their products (mainly eggs) often come from small household production and pose a risk for human health. the second member of this complex, mycobacterium avium subsp. ...201020401832
cell-mediated immune response in swine infected with mycobacterium avium subsp. avium.the zoonotic characteristic of mycobacterium avium subsp. avium (maa) represents a veterinary and economic problem in infected pigs. in this study, we analysed cell-mediated immunity six months after experimental infection by measuring interferon-γ (ifn-γ) production and by performing lymphocyte transformation tests after in vitro re-stimulation with the maa-derived antigen. at the same time, ifn-γ-producing cells were characterised by flow cytometry. in maa-infected animals, the production of i ...201121592586
Displaying items 1 - 11 of 11