| a serological and biochemical study of new field isolates of foot-and-mouth disease virus type a in peru, 1975 to 1981. | three foot-and-mouth disease virus type a isolates recovered from field outbreaks in the department of san martin, peru, during the period 1975 to 1981 were compared with each other, and the south american vaccine strains a24 and a27, by complement fixation (cf), virus neutralization (vn) and polyacrylamide gel electrophoresis (page). complement fixation and vn tests gave comparable results distinguishing the field isolates from each other and from the vaccine strains. analysis of the structural ... | 1983 | 6318421 |
| cross-reactive idiotopes among anti-foot and mouth disease virus neutralizing antibodies. | foot and mouth disease virus (fmdv) viral protein 1 is the only one of the four viral proteins (vp) that induces neutralizing antibodies as an isolated protein. a 32 amino acid (aa) residue (32dimer) of fmdv subtype a12 lp ab vp1 (aa 137-168) was immunogenic against the a12 subtype. three antibody populations each recognizing different epitopes on 32dimer were isolated by affinity chromatography (afc) from the serum of a steer which had been immunized with the 32dimer. the 32dimer contains an aa ... | 1993 | 8406565 |
| foot-and-mouth disease virus: a first inter-laboratory comparison trial to evaluate virus isolation and rt-pcr detection methods. | five european reference laboratories participated in an exercise to evaluate the sensitivity and specificity of their routinely employed rt-pcr tests and cell cultures for the detection and isolation of foot-and-mouth disease (fmd) virus. five identical sets of 20 coded samples were prepared from 10 vesicular epithelia, which were derived from submissions from suspect cases of fmd or swine vesicular disease (svd). sixteen samples were derived from six fmd virus positive epithelia representing fo ... | 2006 | 16846700 |
| use of a baculovirus-expressed structural protein for the detection of antibodies to foot-and-mouth disease virus type a by a blocking enzyme-linked immunosorbent assay. | a blocking enzyme-linked immunosorbent assay (elisa) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type a. it exhibited 99% specificity with a cutoff of 53% inhibition. its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking elisa, indicating its potential as an alternative assay. | 2010 | 19889938 |
| baculovirus treatment fully protects mice against a lethal challenge of fmdv. | foot-and-mouth disease virus (fmdv) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. fmdv is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. it has been demonstrated that fmdv a/arg/2001 causes the death of adult c57bl/6 mice within 72h. we evaluated the capacity of autographa californica nuclear polyhedrosis ... | 2010 | 20580746 |
| develope monoclonal antibody against foot-and-mouth disease virus a type. | in order to develop an anti-fmdv a type monoclonal antibody (mab), babl/c mice were immunized with fmdv a type. monoclonal antibodies (mabs) 7b11 and 8h4 against foot-and-mouth disease virus (fmdv) serotype a were produced by fusing sp2/0 myeloma cells with splenocyte from the mouse immunized with a/av88. the microneutralization titer of the mabs 7b11 and 8h4 were 1024 and 512, respectively. both mabs contain kappa light chains, the mabs were igg1. in order to define the mabs binding epitopes, t ... | 2011 | 21847759 |