an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to pasteurella pneumotropica in murine elisa for the detection of class specific igg antibodies to pasteurella pneumotropica was developed for the serological diagnosis of infections in mouse colonies. heat inactivated whole cell preparations of an isolate of p. pneumotropica biotype heyl (strain p 166) served as antigen for the elisa procedure and for immune serum production in germ-free han:nmri mice. cross reactions with the autochthonous flora of han:nmri spf-mice were not observed, but were evident when a p. pneumotropica ant ...19882966877
[selective media for pasteurella pneumotropica].bacteria from the subcutaneous abscesses which appeared in a laboratory colony of ds mouse since october of 1977 were identified as pasteurella pneumotropica by various biological examinations. the abscess formation was limited to multiparous female mice over 100 day-age, but virgin females were free from the disease. the mic of various antibacterial substances showed that potassium tellurite, kanamycin and bacitracin were effective to isolate the organism selectively from various infection site ...19873678366
reclassification of 30 pasteurellaceae strains isolated from rodents.thirty pasteurellaceae strains isolated from gerbil, guineapig, hamster, mouse, muskrat and rat were reinvestigated and reclassified after comparison with reference strains. strains originally described as pasteurella pneumotropica were reclassified as [pasteurella] pneumotropica heyl biotype (7), [p.] pneumotropica jawetz biotype (1), pasteurella dagmatis (1) or taxon 22 (2). strains previously reported as actinobacillus sp. were reclassified as [p.] pneumotropica biotype jawetz (3), p. dagmati ...19957564217
colonization pattern of pasteurella pneumotropica in mice with latent pasteurellosis.colonization pattern of pasteurella pneumotropica (p. pneumotropica) in mice with latent pasteurellosis was examined with the original selective media, nkbt medium and tgn broth. in the mice of 0 to 15 weeks old, the organism was mainly isolated from the upper respiratory tract, lower intestinal tract, feces and vagina, with the highest isolation rate in the pharyngolarynx. in the pregnant and lactating mice, the organism was isolated from the same sites, but not from the uterus, fetus or mammar ...19947925625
myopathy and spontaneous pasteurella pneumotropica-induced abscess formation in an hiv-1 transgenic mouse an effort to augment human immunodeficiency virus type 1 (hiv-1) gene expression in transgenic mice, an infectious proviral dna clone was modified by deleting the two nf kappa b binding sites and some adjacent upstream ltr sequences and replacing them with the core enhancer of moloney murine leukemia virus (mlv). two independent lines of mlv/hiv transgenic mice were established that expressed hiv-1-specific rna in lymphoid tissue, striated skeletal muscle, and the eye lens. heterozygous anima ...19968862275
outbreak of pasteurella pneumotropica in a closed colony of stock-cd28(tm1mak) mice.fifteen mice with pasteurella pneumotropica orbital abscesses were noted in mice that were homozygous for a targeted cd28 gene mutation. only one mouse heterozygous for the cd28 mutation was affected. according to phenotypic reactions and 16s rdna sequencing, the isolates were most similar to biotype heyl. this article provides evidence for an immunologic basis of susceptibility to p. pneumotropica infection. fifteen mice with pasteurella pneumotropica orbital abscesses were noted in mice that w ...200011178314
survival and transmissibility of pasteurella pneumotropica.survival has been determined for pasteurella pneumotropica on various surfaces found in an animal room at 23+/-1 degrees c and 50+/-10% relative humidity. longest survival (120 min) was found on mouse hair, shortest (< 30 min) on laboratory coat fabric. transmission experiments were performed using sentinel animals in order to evaluate the efficiency of their use for the detection of p. pneumotropica in quarantined mice. in sentinels exposed to infected mice by close contact, p. pneumotropica wa ...200111315166
experimental evaluation of cross-contamination between cryotubes containing mouse 2-cell embryos and murine pathogens in liquid nitrogen has been suspected that embryos stored in liquid nitrogen tanks may become contaminated with murine pathogens, if some pathogens had been introduced to the tanks accidentally. to examine this, we stored tubes containing embryos with tubes containing mouse hepatitis virus (mhv) or pasteurella pneumotropica in liquid nitrogen tanks and examined whether progeny mice derived from the embryos were contaminated with the pathogens or not. after storing for 6 months or 1 year the frozen embryos were ...200312638239
mouse infection models for space flight immunology.several immunological processes can be affected by space flight. however, there is little evidence to suggest that flight-induced immunological deficits lead to illness. therefore, one of our goals has been to define models to examine host resistance during space flight. our working hypothesis is that space flight crews will come from a heterogeneous population; the immune response gene make-up will be quite varied. it is unknown how much the immune response gene variation contributes to the pot ...200516101105
evaluation of antigen panels for elisa monitoring of mouse colonies for antibodies to pasteurellaceae.pasteurellaceae infection in mice may be monitored by the detection of serum antibody using enzyme-linked immunosorbent assay (elisa). we re-evaluated our standard antigen panel comprising pasteurella pneumotropica and a v-factor requiring haemophilus species (strain h21) by studying their serological relationship with actinobacillus muris and 'haemophilus influenzae-murium'. serologically, a. muris and 'h. influenzae-murium' were found to be unrelated and to differ from p. pneumotropica and hae ...200616600079
an outbreak of pasteurella pneumotropica in genetically modified mice: treatment and elimination.a closed breeding colony comprising genetically engineered, wild-type, and stock mice presented with varying degrees of bilateral mucopurulent conjunctivitis and panophthalmitis. the one mouse with unilateral corneal ulceration, a knockout animal, was submitted for necropsy, and bacterial culture samples were obtained from the affected eye and uterus. in addition, ocular swabs from another 12 clinically affected animals, consisting of knockout, transgenic, wild-type, and stock mice, were submitt ...200319757621
molecular and virulence characteristics of an outer membrane-associated rtx exoprotein in pasteurella pneumotropica.pasteurella pneumotropica is a ubiquitous bacterium that is frequently isolated from laboratory rodents and causes various clinical symptoms in immunodeficient animals. currently two rtx toxins, pnxia and pnxiia, which are similar to hemolysin-like high-molecular-weight exoproteins are known in this species. in this study, we identified and analyzed a further rtx toxin named pnxiiia and the corresponding type i secretion system.201121410992
infectious microorganisms in mice (mus musculus) purchased from commercial pet shops in this study, we investigated the prevalence of infectious microorganisms (viruses, bacteria, fungi and eukaryotic parasites) in mice from different pet shops in germany; such animals may compromise the hygienic integrity of laboratory animal vivaria if private pet holders act as unintended vectors of infections carried by them. house mice sold as pets or feed specimens were purchased from different pet shops and tested for a comprehensive panel of unwanted microorganisms. we found a number of ...201121508117
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