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mechanism of interferon action. translational control and the rna-dependent protein kinase (pkr): antagonists of pkr enhance the translational activity of mrnas that include a 161 nucleotide region from reovirus s1 mrna.the interferon-inducible, rna-dependent protein kinase (pkr) is an important regulator of viral protein synthesis. activated pkr inhibits protein synthesis by phosphorylating initiation factor eif-2 alpha. the reovirus s4 gene, whose 1196 nucleotide mrna transcript does not activate the pkr kinase, is efficiently expressed in vector-transfected monkey cos cells. by contrast, the 1463 nucleotide s1 gene of reovirus, which is a potent activator of pkr, is poorly expressed in cos cells. virus genet ...19947526609
effect of the purification of virus antigens on the production of specific complement-fixing antibodies.the effect of viral purification procedures on the antibody response of guinea pigs to immunization with reovirus type 2 and echovirus type 19 was investigated. three grades of antigens were employed: (i) infectious monkey kidney tissue culture fluid (tcf), (ii) virus sedimented in the ultracentrifuge and suspended in phosphate-buffered saline, and (iii) virus purified by centrifugation in cscl density gradients. the antibody response of the guinea pigs was studied by the hemagglutination inhibi ...19714995851
isolation and biochemical characterization of the mammalian reovirus type 3 cell-surface receptor.a cell-surface receptor for the mammalian reovirus type 3 hemagglutinin was isolated by using antiidiotypic anti-receptor antibodies. the receptor is a glycoprotein with a molecular mass of 67,000 daltons and a pi of 5.9. evidence that the isolated structure represents the reovirus receptor was obtained by electrophoretic immunoblot studies, which demonstrated that the 67,000-dalton glycoprotein is the only cell-surface structure recognized by both reovirus type 3 and the anti-receptor immunoglo ...19853871949
biosynthesis of reovirus-specified polypeptides: expression of reovirus s1-encoded sigma 1ns protein in transfected and infected cells as measured with serotype specific polyclonal antibody.polyclonal monospecific antibody was prepared against the reovirus serotype 1 lang strain nonstructural sigma 1ns protein encoded by the s1 gene. the antibody was serotype-specific. the sigma 1ns protein of reovirus serotype 1, but not reovirus serotype 3, was recognized by the polyclonal antibody in both immunoprecipitation and western immunoblot assays. the sigma 1ns protein expressed in vector-transfected cos cells was indistinguishable by immunoprecipitation and immunoblot analyses from the ...19911962444
induction of diarrhea in colostrum-deprived newborn rhesus monkeys with the human reovirus-like agent of infantile gastroenteritis.diarrhea developed in five newborn rhesus monkeys (macaca mulatta) inoculated orally on the first day of life with the human reovirus-like agent of infantile gastroenteritis. incubation period ranged from 2-5 days; virus particles were detected in stools in association with illness, and virus shedding lasted between 1 and 3 days. virus derived from monkeys that developed illness following inoculation was infectious for other monkeys but did not induce diarrhea which could be associated temporall ...1976816334
[effect of sodium salicylate on the "in vitro" development of rna viruses (author's transl)].sodium salicylate 10(-3) m is able to limit the number of plaques induced by rna viruses on monkey kidney cell cultures (cercopithecus aethiops and macaccus rhesus). however, the inhibition rate changes with the virus type or even the strain used. poliovirus 2, coxsackie b3, echovirus and reovirus 1 were the most susceptible to the inhibitory effect of the drug. latent infection caused by sv5 decreases the antiviral activity of salicylate. the stages of the poliovirus type 2 growth affected by s ...1975171988
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