| expression of heat shock protein 83 in leishmania is regulated post-transcriptionally. | mechanisms for regulation of heat shock protein (hsp) 83 expression were examined in leishmania amazonesis. transcripts of hsp83 accumulated upon temperature elevation; however, in contrast to non-protozoan eukaryotes (i.e. drosophila, yeast, avian or human cells), no transcriptional activation was observed. the increase in the hsp83 mrna level evolved from temperature induced variations in mrna turn-over: the hsp83 transcript was rapidly degraded at normal temperatures, whereas heat shock led t ... | 1994 | 8078527 |
| stable expression of the bacterial neor gene in leishmania enriettii. | molecular genetic studies in parasitic protozoa have been hindered by the lack of methods for the introduction and expression of modified or foreign genes in these organisms. two recent reports described the transient expression of the bacterial chloramphenicol acetyl transferase (cat) gene under the control of parasite-specific sequences. we now describe the stable expression of a selectable marker, the gene for neomycin resistance (neor) in leishmania enriettii. a chimaeric gene containing the ... | 1990 | 2300209 |
| transfection of leishmania enriettii and expression of chloramphenicol acetyltransferase gene. | we report a transient expression transfection system in leishmania enriettii. a hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (cat; ec 2.3.1.28) gene is expressed after transfection of l. enriettii with the hybrid plasmid. the expression of the cat gene is dependent on the presence of sequences from the alpha-tubulin gene. the hybrid gene is also active in leishmania braziliensis and leishmania major. | 1989 | 2594753 |
| characterization of the cofactor-independent phosphoglycerate mutase from leishmania mexicana mexicana. histidines that coordinate the two metal ions in the active site show different susceptibilities to irreversible chemical modification. | phosphoglycerate mutase (pgam) activity in promastigotes of the protozoan parasite leishmania mexicana is found only in the cytosol. it corresponds to a cofactor-independent pgam as it is not stimulated by 2,3-bisphosphoglycerate and is susceptible to edta and resistant to vanadate. we have cloned and sequenced the gene and developed a convenient bacterial expression system and a high-yield purification protocol. kinetic properties of the bacterially produced protein have been determined (3-phos ... | 2004 | 15096219 |
| structural and functional characterization of the leishmania amazonensis ribosomal rna promoter. | the promoter region of the ribosomal rna (rrna) genes of leishmania amazonensis was characterised and the transcription start point, defined by primer extension, was shown to be a t residue, 1048 nucleotides upstream of the beginning of the 18s sequence. a repetitive element of 60 bp was identified in the intergenic spacer. this element did not show sequence similarity with the region around the transcription start point. conserved sequences were found in the external transcribed spacer of l. am ... | 1996 | 8920010 |
| eight cases of feline cutaneous leishmaniasis in texas. | leishmaniasis is a zoonotic disease caused by intracellular leishmania protozoa that are transmitted by sandflies. the disease occurs in 3 forms: cutaneous, mucocutaneous, and visceral. cutaneous leishmaniasis has been reported in cats in europe and south america and in 1 cat from texas. leishmania mexicana is endemic in texas and has been reported to cause cutaneous lesions in humans. this article describes the pathology of 8 biopsy cases of feline cutaneous leishmaniasis presented to the texas ... | 2010 | 20826847 |