Publications

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the problem of interaction of shigella with epithelial cells.a review and analysis of data on the investigation of factors concerning the initial events of interaction of shigella with epithelial cells: attachment of penetration are presented. in the experiments with 3h-labeled bacteria it was shown that although common pili confer adhesive properties to bacteria but the penetration of shigella into the cell is not increased. it was stated that lps of shigella o antigen plays an important role not only in interaction with cellular and humoral factors of t ...1979442880
myosin-cross-reactive epitope of shigella flexneri invasion plasmid antigen b.ipab, invasion plasmid antigen b, of shigella flexneri is a 62-kda protein required for invasion of intestinal epithelial cells. ipab is also one of several major protein antigens recognized by the humoral immune systems of most humans and monkeys after infection with shigellae. computer analysis of the deduced ipab amino acid sequence indicates that an alpha-helical structure is likely through much of the molecule. homology searches with protein data banks show that one alpha-helical domain bet ...19921370431
apoptosis of murine peritoneal macrophages induced by an avian pathogenic strain of escherichia coli.the mechanisms used by avian strains of escherichia coli to invade the respiratory epithelia, leading to septicemia in poultry, are not well-established. in this work, we show that resident murine peritoneal macrophages infected in vitro with an avian strain of e. coli underwent apoptosis 4 h after infection (55.6% of apoptosis in infected cells versus 3.5% in non-infected cells). heat-inactivated bacteria did not induce apoptosis and the inhibition of phagocytosis by pretreatment of cells with ...199910481089
differential expression of inducible nitric oxide synthase is associated with differential toll-like receptor-4 expression in chicken macrophages from different genetic backgrounds.the purpose of this study was to examine inos gene expression and activity in macrophages from different chicken genetic lines against various bacterial lps. furthermore, the possible involvement of surface lps receptors as candidates for differential inos gene induction in these genetic lines of chicken was also examined. sephadex-elicited abdominal macrophages (1 x 10(6)) as well as inos hyper-responder macrophages from a transformed chicken macrophage cell line, mq-ncsu, were exposed to 5 mic ...200211777534
patterns and properties of haemagglutinins expressed by shigella serogroups in lagos, nigeria.forty-five strains of shigella were screened for haemagglutinin production and broad-spectrum haemagglutination reaction. mannose-sensitive haemagglutinin (msha) was found in 22 strains [shigella flexneri (7), s. dysenteriae (7), s. sonnei (3), and s. boydii (5)]. eighteen strains harboured mannose-resistant haemagglutinin (mrha), and 8 strains were observed to be non-haemagglutinating to guinea pig erythrocyte. with the exception of human erythrocytes (o, a, b, and ab), the observed msha and mr ...200313677435
a sitabcd homologue from an avian pathogenic escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide.an operon encoding a member of the family of atp-binding cassette (abc) divalent metal ion transporters, homologous to salmonella enterica sitabcd, has been identified in the avian pathogenic escherichia coli (apec) strain chi7122. the sitabcd genes were located on the virulence plasmid papec-1, and were highly similar at the nucleotide level to the chromosomally encoded sitabcd genes present in shigella spp. a cloned copy of sitabcd conferred increased growth upon a siderophore-deficient e. col ...200616514154
plasmid-mediated quinolone resistance conferred by qnrs1 in salmonella enterica serovar virchow isolated from turkish food of avian origin.to study the molecular characteristics of the quinolone and associated ampicillin resistance mechanisms present in salmonella enterica serovar virchow isolated from turkish foods.200717881633
phenotypic and molecular characterization of tem-116 extended-spectrum beta-lactamase produced by a shigella flexneri clinical isolate from chickens.extended-spectrum beta-lactamases (esbls) produced by a clinical isolate of shigella flexneri from chickens were detected with confirmatory phenotypic tests of the clinical and laboratory standards institute, and minimum inhibitory concentrations of several antibacterial drugs against the isolate were determined by the twofold dilution method. the genotype and subtype of the esbl-producing s. flexneri isolate were identified by pcr amplifying of esbl genes and dna sequencing analysis. the result ...200818093137
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