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actinobacillus rossii sp. nov., actinobacillus seminis sp. nov., nom. rev., pasteurella bettii sp. nov., pasteurella lymphangitidis sp. nov., pasteurella mairi sp. nov., and pasteurella trehalosi sp. nov.evidence from numerical taxonomic analysis and dna-dna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. the following new species are proposed: actinobacillus rossii sp. nov., from the vaginas of postparturient sows; actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; pasteurella bettii sp. nov., associated with human bartholin gland abscess and finger infections; pasteurella lymphangitidis sp. nov. (the blg group), w ...19902223608
divergent activity and function of superoxide dismutases in pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10.representative strains of pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10 were examined for the presence of superoxide dismutase. visualisation of superoxide dismutase enzyme activity on polyacrylamide gels, and specific inhibition with potassium cyanide verified a copper/zinc (cu/zn) superoxide dismutase only in serotype a2 whereas serotypes a1 and t10 showed other superoxide dismutase activity. using a simple freeze-thaw method the cellular location of supero ...19979170262
serotypes of pasteurella haemolytica and pasteurella trehalosi isolated from farm animals in hungary.the biochemical and serological characteristics of 486 p. haemolytica and 31 p. trehalosi strains (517 in total) isolated from different lesions of cattle, sheep, goats, pigs and poultry were examined. a total of 476 p. haemolytica strains (97.9%) showed the characteristics typical of the former biotype a of p. haemolytica, while 10 isolates (2.1%), all from poultry, could not be biotyped. a total of 481 strains (93.0%) could be assigned to one of the 17 serotypes of p. haemolytica-p. trehalosi ...199910379234
conservation of expression and n-terminal sequences of the pasteurella haemolytica 31-kilodalton and pasteurella trehalosi 29-kilodalton periplasmic iron-regulated proteins.this study examined the conservation of expression of a 31-kda iron-regulated protein by serotypes of pasteurella haemolytica and pasteurella trehalosi associated with pasteurellosis of cattle and sheep. a polyclonal antibody prepared against the purified 31-kda periplasmic iron-regulated protein from p. haemolytica serotype a1 showed that all p. haemolytica serotypes expressed similar 31-kda proteins with identical n-terminal sequences, whereas p. trehalosi serotypes expressed immunologically d ...199910391874
sequence diversity and molecular evolution of the leukotoxin (lkta) gene in bovine and ovine strains of mannheimia (pasteurella) haemolytica.the molecular evolution of the leukotoxin structural gene (lkta) of mannheimia (pasteurella) haemolytica was investigated by nucleotide sequence comparison of lkta in 31 bovine and ovine strains representing the various evolutionary lineages and serotypes of the species. eight major allelic variants (1.4 to 15.7% nucleotide divergence) were identified; these have mosaic structures of varying degrees of complexity reflecting a history of horizontal gene transfer and extensive intragenic recombina ...200111157953
studies on the production of quorum-sensing signal molecules in mannheimia haemolytica a1 and other pasteurellaceae species.the bioluminescence assay system using vibrio harveyi reporter strains were used to examine quorum-sensing autoinducer (ai) activity from mannheimia haemolytica a1 cell-free culture supernatant. we showed that m. haemolytica a1 cell-free culture supernatant contains molecules that can stimulate the quorum-sensing system that regulates the expression of the luciferase operon in v. harveyi. specifically, m. haemolytica a1 can stimulate only the quorum system 2 but not system 1, suggesting that the ...200211786252
novel protease produced by a pasteurella trehalosi serotype 10 isolate from a pneumonic bighorn sheep: characteristics and potential relevance to protection.a strain of pasteurella trehalosi serotype 10, e(co)-100, isolated from a bighorn sheep that had succumbed to pneumonic pasteurellosis during an epizootic, was compared to well-characterized strains of p. trehalosi serotype 10 and mannheimia haemolytica serotype 1. the gene for leukotoxin a (lkta) from e(co)-100 was sequenced and found to be identical on an amino acid basis to a published sequence for lkta from p. trehalosi serotype 10. however, the toxic activity in culture supernatant measured ...200312637002
plasmid-mediated florfenicol resistance in pasteurella trehalosi.a florfenicol-resistant pasteurella trehalosi isolate from a calf was investigated for the presence and the location of the gene flor.200616670108
microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis).a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ...200718229860
antimicrobial susceptibility patterns and sensitivity to tulathromycin in goat respiratory bacterial isolates.bacterial pneumonia is a common and often life-threatening respiratory problem in both meat and dairy goats. options for approved antibiotic therapy in goats to combat these bacterial infections are severely limited and frequently drugs must be used in an extra-label manner. tulathromycin, a triamilide macrolide antimicrobial drug shown to be effective against swine and cattle respiratory bacterial agents, has been identified as a potentially useful drug in caprines. the present study was conduc ...201122078276
serotypes a1 and a2 of mannheimia haemolytica are susceptible to genotypic, capsular and phenotypic variations in contrast to t3 and t4 serotypes of bibersteinia (pasteurella) trehalosi.mannheimia haemolytica and bibersteinia (pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. the disease is determined by specific serotypes found in cattle and small ruminants. the molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. this study aimed to detect the instability and variations of bacterial strains that may affect the analysis of epide ...200818179587
reclassification of [pasteurella] trehalosi as bibersteinia trehalosi gen. nov., comb. nov.[pasteurella] trehalosi is an important pathogen of sheep, being primarily associated with serious systemic infections in lambs but also having an association with pneumonia. the aim of the present investigation was to characterize a broad collection of strains tentatively identified as [p.] trehalosi in order to reclassify and rename this taxon to support improvements in our understanding of the pathogenesis and epidemiology of this important organism. the type strain for [p.] trehalosi, strain ...200717392184
diversity of temperate bacteriophages induced in bovine and ovine mannheimia haemolytica isolates and identification of a new p2-like phage.the diversity of temperate bacteriophages was examined in 32 mannheimia haemolytica, six mannheimia glucosida and four pasteurella trehalosi isolates. phage particles were induced and identified by electron microscopy in 24 (75%) m. haemolytica isolates, but in only one (17%) m. glucosida and one (25%) p. trehalosi isolate. the m. haemolytica phages were relatively diverse as seven siphoviridae, 15 myoviridae and two podoviridae-like phages were identified; the myoviridae-type phages also exhibi ...200616842340
sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompa) of mannheimia(pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi.the ompa (or heat-modifiable) protein is a major structural component of the outer membranes of gram-negative bacteria. the protein contains eight membrane-traversing beta-strands and four surface-exposed loops. the genetic diversity and molecular evolution of ompa were investigated in 31 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains by comparative nucleotide sequence analysis. the ompa proteins of m. haemolytica and m. glucosida contain four h ...200415317779
mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) in mannheimia (pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi.the mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) was investigated by nucleotide sequence comparison of the lktc, lktb, and lktd genes in 23 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains. sequence variation in the lkta gene has been described previously (r. l. davies et al., j. bacteriol. 183:1394-1404, 2001). the leukotoxin operon of m. haemolytica has a complex mosaic structure and has been derived by extensive i ...200211741868
evaluation of ewe vaccination as a tool for increasing bighorn lamb survival following pasteurellosis epizootics.we conducted field and laboratory experiments to evaluate whether treating pregnant bighorn ewes with a combination of an experimental pasteurella trehalosi and mannheimia haemolytica (formerly p. haemolytica) vaccine and a commercially-available bovine p. multocida and m. haemolytica vaccine would increase lamb survival following a pneumonia epidemic. three free-ranging bighorn herds affected by pasteurellosis outbreaks between november 1995 and june 1996 were included in the field experiment. ...200111272504
phylogenetic relationships and diversity within the pasteurella haemolytica complex based on 16s rrna sequence comparison and outer membrane protein and lipopolysaccharide analysis.the outer membrane protein (omp) and lipopolysaccharide (lps) profiles of 30 untypeable isolates of pasteurella haemolytica were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compared with the profiles of typeable isolates. the phylogenetic relationships of 28 isolates representing each of the serotypes of p. haemolytica and pasteurella trehalosi, as well as untypeable isolates of p. haemolytica, were determined by comparing 16s rrna sequences. the analysis of the omp ...19968782683
a bighorn sheep die-off in southern colorado involving a pasteurellaceae strain that may have originated from syntopic cattle.we investigated a pasteurellosis epizootic in free-ranging bighorn sheep (ovis canadensis) wherein a pasteurellaceae strain carried by syntopic cattle (bos taurus) under severe winter conditions appeared to contribute to pneumonia in affected bighorns. twenty-one moribund or dead bighorn sheep were found on the "fossil ridge" herd's winter range, colorado, usa, between 13 december 2007 and 29 february 2008. eight carcasses examined showed gross or microscopic evidence of acute to subacute fibrin ...201020966277
evidence for a common gene pool and frequent recombinational exchange of the tbpba operon in mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi.the tbpba operon was sequenced in 42 representative isolates of mannheimia haemolytica (32), mannheimia glucosida (6) and bibersteinia trehalosi (4). a total of 27 tbpb and 20 tbpa alleles were identified whilst the tbpba operon was represented by 28 unique alleles that could be assigned to seven classes. there were 1566 (34.8% variation) polymorphic nucleotide sites and 482 (32.1% variation) variable inferred amino acid positions among the 42 tbpba sequences. the tbpba operons of serotype a2 m. ...201020884693
bibersteinia trehalosi necrotising hepatitis associated with sudden death in an adult cow. 201020643888
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