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retrospective study of an ehrlichia canis epizootic around phoenix, arizona.antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 40 of 339 dogs (12%) examined from around phoenix, az, during 1975. the prevalence was higher in purebred and crossbred german shepherd dogs (21%), as compared with 8% in the remaining breeds. the difference in antibody prevalence between sexes was not significant. dogs 12 weeks to 13 years old were seropositive. the causative agent was isolated by inoculation of whole blood from a seropositive dog into a sus ...1978624663
effect of canine immune macrophages and canine immune serum on the growth of ehrlichia canis.the effect of canine immune macrophages and canine immune serum (cis) on the growth of ehrlichia canis was studied in vitro. treatment of e canis organisms with cis before they were inoculated into immune macrophage cultures, coupled with pre- and postinoculation maintenance of cultures in cis, suppressed macrophage infection rates to 1% or less. immune macrophages collected from e canis-carrier dogs and maintained in canine normal serum were more resistant to the intracellular growth of e canis ...1978629452
neurologic manifestations of trypanosomiasis in a dog.a 13-month-old doberman pinscher was evaluated because of slowly progressive paraparesis and signs of depression. the dog had temporal, supraspinatus, and infraspinatus muscle atrophy, bilateral enophthalmos, superficial inguinal lymphadenopathy, tachycardia with pulse deficits, and lesions of active and inactive chorioretinitis. neurologic abnormalities included hyperreflexic patellar reflexes, lack of conscious proprioception, signs of superficial pain in the hind limbs, and depressed hopping ...19911899857
evaluation of rhipicephalus sanguineus as a potential biologic vector of ehrlichia platys.the brown dog tick, rhipicephalus sanguineus (acari: ixodidae), transmits several diseases among dogs including ehrlichia canis infection. the role of rhipicephalus sanguineus as a biologic vector for e platys, the rickettsial agent of infectious canine cyclic thrombocytopenia, was studied in dogs. laboratory-cultured, pathogen-free nymph ticks were fed to repletion on dogs acutely infected with e platys. tick engorgement coincided with the development of initial parasitemia and thrombocytopenia ...19911952347
serologic diagnosis of human ehrlichiosis using two ehrlichia canis isolates.ehrlichia canis or a closely related rickettsial organism has been implicated serologically and morphologically as the causative agent of human ehrlichiosis in the united states. although e. canis has been serially propagated in primary canine monocytes, only a limited quantity of antigen is obtained by this method. a continuous canine macrophage cell line, dh82, supports the growth of a new isolate of e. canis established from the whole blood of a carrier dog in oklahoma. serologic comparison o ...19911995730
serological evaluation of ehrlichia canis infections in military dogs in africa and reunion island.sixty-eight dogs from four african countries and reunion island were tested for antibodies against ehrlichia canis. twenty-six dogs (50%) in tunisia, senegal and chad were found positive using the indirect fluorescence antibody test. dogs from both the central african republic and reunion island were all negative. thus, this preliminary report confirms the presence of e. canis in africa. larger studies will be necessary to evaluate the current epidemiologic situation of canine ehrlichiosis in th ...19912024432
immunohistologic demonstration of ehrlichia canis. 19902165753
failure to transmit ehrlichia canis (rickettsiales: ehrlichieae) with otobius megnini (acari: argasidae).an ear tick, otobius megnini (dugès) recovered from a child who had serologic evidence of ehrlichiosis, was examined for ehrlichia species microscopically and by inoculation into a susceptible dog; no evidence of infection was found in the tick. experimental transmission of e. canis by laboratory-reared o. megnini was attempted; neither transstadial nor transovarial transmission occurred.19902231616
canine ehrlichiosis in connecticut.the first case of canine ehrlichiosis in connecticut is reported. a female brittany spaniel from milford presented with lethargy, anorexia, fever, petechiae, splenomegaly, thrombocytopenia, anemia, elevated serum alkaline phosphatase, lymphopenia, and hypoalbuminemia. serologic analysis revealed antibodies to ehrlichia canis (titer, 1:2,560). this documents a more northern geographic distribution in the united states for this infectious agent than had previously been suspected.19902312682
adaptation of ehrlichia sennetsu to canine blood monocytes: preliminary structural and serological studies with cell culture-derived ehrlichia sennetsu.ehrlichia sennetsu, the causative agent of human sennetsu rickettsiosis, was successfully propagated in primary canine blood monocyte cultures. the growth cycle of this organism appears to be similar to that of ehrlichia canis. the antigen derived from our e. sennetsu cultures was used to develop an indirect fluorescent antibody test for detection and titration of serum antibodies to the organism. using this test system, we found that five human serum samples obtained from patients clinically di ...19852985504
a new strain of ehrlichia canis. 19715128792
attempted transmission of canine ehrlichiosis to the vervet monkey (cercopithecus pygerythrus).blood from a domestic dog infected with ehrlichia canis was injected into 3 adult vervet monkeys cercopithecus pygerythrus. the monkeys did not develop any clinical or haematological signs of disease, nor did they seem to be able to harbour the parasite. furthermore, neither morulae of e. canis nor haematological evidence of ehrlichiosis were seen after these monkeys had been splenectomized. the splenectomized monkeys did, however, develop a severe parasitaemia of their red cells with entopolypo ...19817312306
free-living jackals (canis mesomelas)-potential reservoir hosts for ehrlichia canis in kenya.using a modified cell culture test, ehrlichia canis was found in eight of 15 free-living jackals (canis mesomelas) and 14 of 31 dogs owned by pastoral communities in the same areas of kenya. two cross-bred puppies inoculated with blood from infected jackals developed mild, transient clinical disease, and e. canis was recovered from the puppies. tick species found on the jackals were similar to those found on the infected dogs. ehrlichia canis was not found in eight spotted hyaenas (crocuta crocu ...19807463598
further evidence for the efficacy of imidocarb dipropionate in the treatment of ehrlichia canis infection.three dogs experimentally infected with ehrlichia canis developed thrombocytopaenia and high antibody titres to e. canis in indirect fluorescent antibody tests. one dog also became leukopaenic. at weeks 6 and 8 post-infection, the dogs were treated with imidocarb dipropionate (5 mg kg-1 subcutaneously) and a further dose was administered at week 12 (5 mg kg-1 intramuscularly). twelve weeks after the last treatment (post-treatment), all dogs had normal platelet counts which persisted for a furthe ...19947595915
comparison of pcr with other tests for early diagnosis of canine ehrlichiosis.the purpose of the study was to compare the sensitivity of pcr with those of cell culture reisolation of ehrlichia canis, the indirect fluorescent antibody test (ifa), and western immunoblotting (wi) in the early diagnosis of canine ehrlichiosis. five german shepherd dogs were intravenously inoculated with 10(7) e. canis-infected dh82 cells. blood was collected on alternate days during a 2-week postinoculation period. mononuclear cell fractions were harvested and used for e. canis reisolation an ...19947929754
c-reactive protein and alpha 1-acid glycoprotein levels in dogs infected with ehrlichia canis.to elucidate whether acute-phase protein responses occur in dogs infected with ehrlichia canis, c-reactive protein (crp) and alpha 1-acid glycoprotein (aag) levels were serially measured in the plasma of five dogs experimentally inoculated with e. canis and 10 sham-inoculated or noninoculated control dogs. the crp concentration was measured by a canine-specific capture enzyme-linked immunosorbent assay, and the aag concentration was measured by a canine-specific radial immunodiffusion method. in ...19948027343
serological evidence for antigenic relationships between ehrlichia canis and cowdria ruminantium.the sera from dogs experimentally infected with ehrlichia canis reacted at very similar titres in indirect fluorescent antibody (ifa) tests with e canis and cowdria ruminantium antigen. in western blots these sera reacted strongly with the immunodominant antigens of e canis (27 kda) and c ruminantium (25 and 32 kda). the dogs experimentally infected with c ruminantium showed no clinical evidence of infection, though the presence of organisms in the blood was demonstrable, for up to two weeks aft ...19948191006
the first isolation, in vitro propagation, and genetic characterization of ehrlichia canis in israel.ehrlichia canis, the etiologic agent of canine ehrlichiosis, was isolated in israel from a naturally infected dog with acute signs of the disease. the organism designated e. canis 611, was passaged experimentally to a beagle, from which it was propagated in primary canine monocytes. the organism was then grown in vitro in a continuous canine cell line, dh82. nine beagles subsequently injected with whole e. canis-infected blood all developed typical symptoms of ehrlichiosis. an indirect immunoflu ...19968686178
canine monocytic ehrlichiosis: a retrospective study of 100 cases, and an epidemiological investigation of prognostic indicators for the disease.one hundred cases of monocytic ehrlichiosis diagnosed in israeli dogs were confirmed by the presence of anti-ehrlichia canis indirect immunofluorescent antibody titres greater than 1:40. the disease occurred in all age groups and there was no sex predilection. german shepherd dogs were significantly over-represented whereas crossbreed dogs were significantly under-represented (p > 0.0005). the most common clinical signs were depression, lethargy, lymphadenomegaly, fever, anorexia, panting, pale ...19979351183
molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein of ehrlichia canis: a potential serodiagnostic antigen.a gene encoding a 28-kda protein of ehrlichia canis was cloned, sequenced, and expressed, and a comparative molecular analysis with homologous genes of e. canis, cowdria ruminantium, and ehrlichia chaffeensis was performed. the complete gene has an 834-bp open reading frame encoding a protein of 278 amino acids with a predicted molecular mass of 30.5 kda. an n-terminal signal sequence was identified, suggesting that the protein undergoes posttranslational modification to a mature 27.7-kda protei ...199910225842
thrombocytopathia and light-chain proteinuria in a dog naturally infected with ehrlichia canis.a 6-year-old dog was presented for evaluation of recurrent epistaxis. platelet counts, biochemical tests, and coagulation tests were within the normal range, but a mucosal bleeding time was prolonged; there was hyperproteinemia and a monoclonal gammopathy. heterogeneity of light chains appeared in urine, however, thus suggesting that the gammopathy was polyclonal. platelet aggregation tests showed decreased responsiveness to collagen. an ehrlichia canis indirect fluorescent-antibody titer was po ...19979348500
comparison of nested pcr with immunofluorescent-antibody assay for detection of ehrlichia canis infection in dogs treated with doxycycline.a partial 16s rrna gene was amplified in ehrlichia canis-infected cells by nested pcr. the assay was specific and did not amplify the closely related ehrlichia chaffeensis, ehrlichia muris, neorickettsia helminthoeca, and sf agent 16s rrna genes. the assay was as sensitive as southern hybridization, detecting as little as 0.2 pg of e. canis dna. by this method, all blood samples from four dogs experimentally infected with e. canis were positive as early as day 4 postinoculation, which was before ...19979196207
characterization of the subclinical phase of canine ehrlichiosis in experimentally infected beagle dogs.beagle dogs were examined during the subclinical phase of canine ehrlichiosis under controlled conditions. emphasis was placed on gathering data before artificial inoculation with ehrlichia canis, and comparing these data with those of the subclinical phase of the disease. in this study all dogs were clinically healthy throughout the 6 month examination period. all subclinically infected dogs had ifa antibody titers to e. canis at a dilution varying from 1:2560 to 1:20480. the most prominent hae ...19979195740
ocular histopathology of ehrlichial infections in the dog.histologic examination of eyes and brains of 27 dogs experimentally infected with either ehrlichia canis, e. ewingii, e. chaffeensis, or human granulocytic ehrlichia (hge) was conducted in the course of several experiments, the primary objectives of which were to investigate the susceptibility of the domestic dog to infection with various ehrlichial species and to assess the ability of ixodid tick species to acquire and transmit those infections. uveitis and meningitis occurred in each of the do ...200111199163
immunoblot analysis of the immunoglobulin g response to ehrlichia canis in dogs: an international survey.historically, considerable variation has been reported in the type and severity of clinical and hematologic abnormalities associated with canine ehrlichiosis. because of difficulties associated with the isolation of intracellular monocytic ehrlichia species in tissue culture systems, few e. canis isolates are available for comparative microbiologic studies. to address the issue of potential e. canis antigenic diversity in different regions of the world, dog sera reactive by indirect fluorescent ...19979087922
antibodies to selected canine pathogens and infestation with intestinal helminths in golden jackals (canis aureus) in israel.blood and fecal samples, collected from 46 healthy adult free-ranging golden jackals captured in two different locations in israel, were examined. a serological study was conducted to investigate the prevalence of circulating antibodies reacting with four common canine pathogens: canine distemper virus (cdv), canine parvovirus (cpv), ehrlichia canis and leishmania infantum. faecal flotation and haematological tests were also performed. the seroprevalence of cpv, e. canis, cdv, and l. infantum we ...200111409931
reisolation of ehrlichia canis from blood and tissues of dogs after doxycycline treatment.we present evidence that supports the carrier status of dogs experimentally infected with ehrlichia canis after treatment with doxycycline. canine ehrlichiosis was induced in five dogs by intravenous inoculation with e. canis-infected dh82 cells. all animals developed mild clinical signs of transient fever, body weight loss, thrombocytopenia, and increased gamma globulin levels in plasma. an indirect fluorescent-antibody test (ifa) revealed that all dogs had seroconverted (titer, 5,120) by day 1 ...19947929751
detection of ehrlichial infection by pcr in dogs from yamaguchi and okinawa prefectures, japan.species-specific nested polymerase chain reaction (pcr) was used to detect the presence of possible canine ehrlichial agents (ehrlichia canis, e. chaffeensis, e. ewingii, e. equi and e. platys) and monocytic ehrlichial agents found in japan (e. muris and a recently discovered ehrlichia species detected from ixodes ovatus) in blood samples from dogs in yamaguchi and okinawa prefecture, japan. partial sequence of e. platys was detected from 1 of 67 dogs (1.5%) tested from yamaguchi prefecture and ...200111503912
aortic valve endocarditis in a dog due to bartonella clarridgeiae.we report the first documented case of endocarditis associated with bartonella clarridgeiae in any species. b. clarridgeiae was identified as a possible etiological agent of human cat scratch disease. infective vegetative valvular aortic endocarditis was diagnosed in a 2.5-year-old male neutered boxer. historically, the dog had been diagnosed with a systolic murmur at 16 months of age and underwent balloon valvuloplasty for severe valvular aortic stenosis. six months later, the dog was brought t ...200111574571
detection of platelet-bound and serum platelet-bindable antibodies for diagnosis of idiopathic thrombocytopenic purpura in dogs.the sensitivity and specificity of 2 antibody tests for diagnosis of idiopathic thrombocytopenic purpura (itp) in dogs were investigated prospectively. an elisa to detect antibodies bound to the surface of platelets from affected dogs (direct test) was performed in 34 dogs with a clinical diagnosis of itp and in 21 dogs with thrombocytopenia attributable to other causes. an elisa to detect platelet-bindable antibodies in serum from affected dogs (indirect test) was performed in 32 dogs with itp ...19957744662
presence of immune-complexes, and absence of antinuclear antibodies, in sera of dogs naturally and experimentally infected with ehrlichia canis.antinuclear antibodies (ana), immunoglobulin g (igg) concentrations and circulating immune-complexes (cic) were measured, over a period of 3 years, in 6 dogs experimentally infected with ehrlichia canis, and in 10 dogs naturally infected with the rickettsia. no ana were detected in any of the samples tested. the igg concentrations were shown to be higher in the infected dogs when compared to the control dogs. cic were detected in 2 of 10 naturally and 2 of 6 experimentally infected dogs, during ...200111600268
identification and functional analysis of an immunoreactive dsba-like thio-disulfide oxidoreductase of ehrlichia spp.novel homologous dsba-like disulfide bond formation (dsb) proteins of ehrlichia chaffeensis and ehrlichia canis were identified which restored dsba activity in complemented escherichia coli dsba mutants. recombinant ehrlichia dsb (edsb) proteins were recognized by sera from e. canis-infected dogs but not from e. chaffeensis-infected patients. the edsb proteins were observed primarily in the periplasm of e. chaffeensis and e. canis.200211953415
[human ehrlichiosis. review].human ehrlichiosis is a newly recognized tick-borne disease. since 1935 ehrlichia canis has been known as a cause of illness in dogs and other canine species, and for a few years it was related with human disease. in 1990, ehrlichia chaffeensis was isolated from a man suspected of having ehrlichiosis. partial sequencing of the rrnas from the human isolate and e. canis, indicated that they are 98.7% related. more recently (may 1994) an "human granulocytic ehrlichiosis" have been reported in usa. ...19947734523
systemic phaeohyphomycosis caused by xylohypha bantiana in a dog.an 8-year-old, maltese-cross bitch presented with chronic neck and back pain and an acute onset of circling, hyperaesthesia and constant crying. clinical examination revealed temporal muscle atrophy, an abnormal hanging reflex, cervical rigidity and severe hepatomegaly. ultrasonography of the liver showed several disseminated, poorly demarcated, hypoechoic areas which on fine needle aspirates, contained large numbers of pigmented fungal hyphae. cerebrospinal fluid examination revealed fungal hyp ...19947602573
comparison of serological detection methods for diagnosis of ehrlichia canis infections in dogs.we determined the value of four serological assays for the diagnosis of canine monocytic ehrlichiosis by comparing them to the indirect fluorescent-antibody assay "gold standard." the specificity of dip-s-ticks was significantly lower than that of all of the other tests evaluated. the sensitivity of dip-s-ticks was significantly higher than that of snap3dx or the snap canine combo. the sensitivity of the rmap2 enzyme-linked immunosorbent assay (elisa) was significantly higher than that of the sn ...200212202605
evaluation of cytology in the diagnosis of acute canine monocytic ehrlichiosis (ehrlichia canis): a comparison between five methods.the purpose of this study was the comparison of the diagnostic sensitivity between buffy coat (bc), peripheral blood (pb), lymph node (ln), bone marrow (bm) and short-term culture (p-d) cytology that has been based on the detection of ehrlichia canis morulae, in the acute phase of canine monocytic ehrlichiosis (cme). their cellular localization, total numbers and microscopic differentials were also investigated. the highest sensitivities were achieved after evaluating 1000 oil immersion fields ( ...200312458168
systematic review of the distribution of the major vector-borne parasitic infections in dogs and cats in europe.on the basis of a systematic review of the literature, this paper provides the first evidence-based maps of the distribution of the major vector-borne parasitic infections of dogs and cats in europe. from an initial survey of 894 publications, data from 268 were analysed, summarised and collated. prevalence data were used to calculate the force of infection to provide distribution and incidence maps for canine infections with leishmania infantum, dirofilaria immitis, dirofilaria repens, babesia ...200312572938
evaluation of the pathogenic potential of rickettsia canada and rickettsia prowazekii organisms in dogs.an unusual pattern of seroreactivity to antigens of rickettsial organisms (rickettsia rickettsii, r rhipicephali, r montana, and r bellii), particularly to r bellii antigen, was detected in 3 dogs during a 2-month period. thus, studies were initiated to clarify the pathogenic potential of the more distantly related rickettsial organisms (r canada and r prowazekii) in dogs. because r bellii are nonpathogenic rickettsiae that share numerous common properties with spotted fever-group and typhus-gro ...19957601694
cloning and characterization of an ehrlichia canis gene encoding a protein localized to the morula membrane.a gene encoding a 23.5-kda ehrlichial morula membrane protein designated mmpa was cloned by screening an ehrlichia canis expression library with convalescent dog sera, which resulted in three positive clones. sequence analysis of the insert dnas from all three clones indicated an open reading frame with a size of 666 bp that encodes mmpa. the structural analysis of mmpa indicated that it is a transmembrane protein with extreme hydrophobicity. southern blot analysis of the hindiii-digested chromo ...200312654844
cloning and characterization of putative zinc protease genes of ehrlichia canis.a putative zinc protease gene operon from ehrlichia canis was cloned and sequenced. a genomic library was constructed in a phg165 plasmid vector using sau3a partially digested e. canis chromosomal dna. sequence analysis of the insert dna from this clone indicated two open reading frames with a size of 1314 and 1350 bp that encodes for proa and prob, respectively. based on blast analyses, proa and prob share 20-30% identities with members of the eukaryotic mitochondrial processing peptidase (mpp) ...200312825352
ehrlichia canis infections in dogs on aruba (netherlands antilles). 195713428628
sequence and expression analysis of virb9 of the type iv secretion system of ehrlichia canis strains in ticks, dogs, and cultured cells.ehrlichia canis virb9 was cloned and expressed. the sequences of virb9 from six geographic locations were identical. virb9 was transcribed by e. canis in dogs, ticks, and cell culture. infected dogs had antibodies to recombinant virb9, indicating that virb9 was produced by e. canis in dogs and was antigenic.200314500531
serum complement and blood platelet adhesiveness in acute canine ehrlichiosis.three dogs inoculated with ehrlichia canis served as infected controls (group i), 3 dogs treated with cobra venom factor (cvf) after inoculation with e canis were the principals (group ii), 3 noninfected dogs were the noninfected controls (group iii), and 1 noninfected dog given cvf was the noninfected, decomplemented control (group iv). thrombocytopenia occurred in all e. canis-inoculated dogs (groups i and ii) within 3 weeks after they were inoculated. however, the severity of the thrombocytop ...19807447122
acute experimental canine ehrlichiosis. ii. sequential reaction of the hemic and lymphoreticular system of selectively immunosuppressed dogs.the lymphoreticular response of dogs to ehrlichia canis infection was studied after immunosuppressive therapy with cyclophosphamide or antilymphocyte serum. immunosuppression did not increase mortality, nor did it prevent or significantly modify the clinical manifestations of acute ehrlichiosis. the clinical, hematologic and serum biochemical characteristics of acute ehrlichiosis appeared seven or more days later in immunosuppressed than in immunologically intact infected dogs. immunosuppressive ...19817257081
cell-mediated and humoral immune responses of german shepherd dogs and beagles to experimental infection with ehrlichia canis.the cell-mediated and the humoral immune responses of 12 german shepherd dogs and 5 beagles inoculated with ehrlichia canis were evaluated. results indicated that specific and nonspecific immunosuppression due to e canis occurred in the german shepherd dogs. canine leukocyte migration-inhibition factor was successfully isolated and shown to be physically and functionally similar to human and guinea pig migration inhibition factor. of the german shepherd dogs, 58% developed positive cell-mediated ...19806989300
ehrlichia canis and tropical canine pancytopaenia. 19725072572
comparison of simultaneous splenic sample pcr with blood sample pcr for diagnosis and treatment of experimental ehrlichia canis infection.this report presents evidence that dogs recover from acute canine monocytic ehrlichiosis (cme) after 16 days of doxycycline treatment (10 mg/kg of body weight every 24 h). blood pcr was as valuable as splenic aspirate pcr for early diagnosis of acute cme. splenic aspirate pcr was, however, superior to blood pcr for the evaluation of ehrlichial elimination.200415504892
characterization of the subclinical phase of ehrlichiosis in dogs.prevalence of subclinical ehrlichia canis infection in a mississippi kennel was 53%. most of the dogs probably had been infected for 4 or 5 years. the subclinical phase of the infection was characterized by high antibody titers to e canis (9 of 10 dogs with titers of 1:5, 120), hyperglobulinemia (9 of 10 dogs), thrombocytopenia (5 of 10), absolute lymphocytosis (4 of 10), and absolute neutropenia (3 of 10). the dogs had normal pcv, serum albumin concentrations, and urine protein excretion. findi ...19863733499
use of plasmapheresis and chemotherapy for treatment of monoclonal gammopathy associated with ehrlichia canis infection in a dog.monoclonal gammopathy associated with ehrlichia canis infection was diagnosed in a german shepherd dog. the dog was treated by use of chemotherapy and tetracycline and by plasmapheresis. the dog tolerated plasmapheresis and long-term drug therapy well, and clinical signs resolved over a 90-day period. the monoclonal gammopathy resolved after treatment, but specific antibody to e canis indicated suppression followed by a rebound to the initial high titer.19873583884
ehrlichia canis: a tick-borne rickettsial-like infection in humans living in the southeastern united states.during the past two years, sporadic cases of a rickettsial-like illness were reported in humans living in the southeastern united states. the illness was serologically similar to ehrlichia canis infections in dogs. it resembled spotless rocky mountain spotted fever but was differentiated from this infection serologically with acute and convalescent sera showing increasing titers to ehrlichia canis. e. canis infection should be suspected in patients with fever, headache, malaise, myalgia, gastroi ...19892643878
a preliminary study to evaluate the immune responses induced by immunization of dogs with inactivated ehrlichia canis organisms.ehrlichia canis is an intracellular pathogen that causes canine monocytic ehrlichiosis. although the role of antibody responses cannot be discounted, control of this intracellular pathogen is expected to be by cell mediated immune responses. the immune responses in dogs immunized with inactivated e. canis organisms in combination with quil a were evaluated. immunization provoked strong humoral and cellular immune responses, which were demonstrable by western blotting and lymphocyte proliferation ...200516137129
suppurative, nonseptic polyarthropathy in dogs.the goals of this study were to determine the historical, physical examination, and clinicopathologic findings in dogs with suppurative, nonseptic polyarthropathy and to identify concurrent disorders associated with this syndrome. medical records of 52 dogs with cytologic evidence of suppurative inflammation in two or more joints were examined retrospectively. age of dogs was 4.8 years (median, range: 0.5-12 years). there was no clear breed or sex predilection, but most were large-breed dogs (bo ...200516231709
canine ehrlichiosis in six dogs with persistently increased antibody titers.chronic ehrlichiosis was diagnosed in six dogs on the basis of increased immunofluorescent antibody titers to ehrlichia canis. although clinical signs varied, all six dogs were anemic, hyperglobulinemic, and an igg monoclonal gammopathy was documented in five dogs in which serum protein electrophoreses were performed. all dogs were treated with tetracycline for at least 14 days; four dogs also received immunosuppressive drugs. clinical signs resolved within 1 week, hematologic abnormalities reso ...19911920256
experimentally induced infection of dogs, cats, and nonhuman primates with ehrlichia equi, etiologic agent of equine ehrlichiosis.dogs (german shepherd dogs and beagles), cates, rhesus macaques (macaca mulatta), and baboons (papio anubis) were inoculated with whrlichia equi, the etiologic agent of equine ehrlichiosis. within 3 to 7 days after inoculation, morulae were observed in the eosinophils of cats, neurtrophils of macaques and baboons, and in both neutrophils and eosinophils of dogs. the severe disease produced in horses by this agent was not a feature of e equi infection in dogs, cats, macaques, and baboons. however ...19751115421
isolation in illinois of a foreign strain of ehrlichia canis, the causative agent of canine ehrlichiosis (tropical canine pancytopenia).ehrlichiosis (tropical canine pancytopenia) was diagnosed in illinois in a 4 1/2-year-old mixed breed dog that had spent the previous 2 years in india. the dog had periodic epistaxis, anemia, leukopenia, hypoalbuminemia, and hypergammaglobulinemia. a titer of 1:160 for ehrlichia canis was detected by the indirect fluorescent antibody test (ifat). subinoculation of blood into a clinically normal dgo resulted in typical signs of canine ehrlichiosis, and e canis was identified in circulating mononu ...19751112743
epizootiology of tropical canine pancytopenia.tropical canine pancytopenia (tcp) is a newly recognized infectious disease of dogs in diverse tropical and subtropical areas. the disease is characterized by hemorrhage, pancytopenia, severe emaciation and persistent infection. dogs with tcp are often presented with epistaxis, which is the most dramatic sign of the disease; however, a large number of affected dogs develop severe pancytopenia and die without manifesting clinical signs of hemorrhage. the disease has been reported most frequently ...197016512116
ehrlichiosis in wolves, dogs, and wolf-dog crosses.canine ehrlichiosis was diagnosed in wolves, dogs, and wolf-dog crosses at a small zoo in northcentral florida. five of 9 adult canids and all 8 pups confined to a common kennel died as a result of the infection. the epizootic was associated with a massive rhipicephalus sanguineus infestation. hematologic and pathologic findings in an adult wolf-dog cross that died were characteristic of canine ehrlichiosis. that animal and four other canids were seropositive for ehrlichia canis. no deaths occur ...1979521367
kinetics of antibody response to ehrlichia canis assayed by the indirect fluorescent antibody method.the kinetics of antibody production response to experimentally induced infection of dogs with ehrlichia canis was determined by ion-exchange and molecularsieve chromatography and by indirect fluorescent antibody (ifa) test. the first ifa antibody at 7 days after inoculation resided in immunoglobulin m (igm) and immunoglobulin a (iga) classes. at approximately 21 days after inoculation, the antibody was in igm, iga, and immunoglobulin g (igg) classes. thereafter, antibody concentrations continued ...197549162
hepatozoon canis infection associated with dog ticks of rural areas of rio de janeiro state, brazil.hepatozoon canis is a tick-borne protozoan that infects dogs and has been reported throughout the world. manifestation of h. canis infection varies from being sub-clinical in apparently healthy dogs to severe illness. the main vector of the infection is the dog tick, rhipicephalus sanguineus although other species may also transmit this agent. h. canis has been reported previously in brazil, but mostly as an occasional finding during laboratory exams and always associated with other diseases. th ...200111113545
canine monocytic ehrlichiosis presenting as acute blindness 36 months after importation into the uk.a four-year-old labrador retriever developed sudden-onset blindness, associated with bilateral uveitis, intraocular haemorrhage and retinal detachment. it had been imported into the uk from sardinia 36 months before presentation. haematological abnormalities included non-regenerative anaemia, thrombocytopenia and neutropenia. serum and urine protein electrophoresis demonstrated a monoclonal gammopathy. an immunofluorescent antibody test for ehrlichia canis was positive, with a titre of 1:320, co ...200010879405
bone marrow mastocytosis in dogs with myelosuppressive monocytic ehrlichiosis (ehrlichia canis): a retrospective study.bone marrow mastocytosis has been reported rarely in naturally occurring canine monocytic ehrlichiosis (cme).200616967415
rickettsial agents in egyptian ticks collected from domestic animals.to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ...200617004028
survey of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture, japan.serological tests were performed to investigate extent of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture. three of 22 dogs (13.6%) were positive for ehrlichia canis. two of 19 dogs (10.5%) were positive for rickettsia japonica. three of 22 dogs (13.6%) were positive for babesia gibsoni. none of these animals were positive for coxiella burnetii or hepatozoon canis. a microfilaria was detected in a drop smear of hemolymph from an engorged femal ...19989673953
amplification of ehrlichial dna from dogs 34 months after infection with ehrlichia canis.in order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (cme) are carriers of ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-e. canis antibody titers during this phase, pcr was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with e. canis. at least one of the three samples (spleen, bone marrow, and blood ...19989431923
significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by ehrlichia canis.dogs are susceptible to a number of ehrlichial diseases. among them, canine monocytic ehrlichiosis is an important and potentially fatal disease of dogs caused by the rickettsia ehrlichia canis. diagnosis of the disease relies heavily on the detection of antibodies and is usually carried out using the indirect immunofluoresence antibody (ifa) test. the ifa test may be confounded by cross-reactivities between a number of the canine ehrlichial pathogens. this article presents a review of the ehrli ...200111163693
molecular survey of ehrlichia canis and anaplasma phagocytophilum from blood of dogs in italy.we investigated the prevalence of ehrlichia canis (e. canis) (n = 601) and anaplasma phagocytophilum (a. phagocytophilum) (n = 460) infection by means of real-time pcr from blood of italian dogs. the prevalence of e. canis in northern, central, and southern italy was 2.9%, 8%, and 9.7%, respectively. the prevalence of a. phagocytophilum was 0%.200617114768
prevalence of ehrlichia canis (rickettsiales: anaplasmataceae) in dogs and rhipicephalus sanguineus (acari: ixodidae) ticks from brazil.the current study evaluated the prevalence of ehrlichia canis donatien and lestoquard in domestic dogs, canis familiaris l., and rhipicephalus sanguineus (latreille) (acari: ixodidae) ticks from different areas of brazil. in monte negro county (state of rondônia, brazilian western amazon), the indirect immunofluorescence assay detected e. canis-reactive antibodies (titer > or = 40) in 58/153 (37.9%) and 40/161 (24.8%) dogs from the urban and rural areas, respectively. these values were significa ...200717294930
comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations.three serological methods, indirect fluorescent immunoassay (ifi), enzyme-linked immunosorbent assay (elisa) and direct agglutination test (dat) that are commonly employed in the diagnosis of canine visceral leishmaniasis (cvl), have been assessed. a total of 234 domestic dogs, drawn from an area in the municipality of belo horizonte, minas gerais, brazil, endemic for visceral leishmaniasis, were submitted to clinical and parasitological examinations and serological assay. sera collected from co ...200717403582
standardization of the diagnostic criteria for canine ehrlichiosis. towards a universal case definition.canine ehrlichiosis is a highly variable syndrome presenting a significant differential diagnostic difficulty. it imitates many metabolic and infectious diseases and lacks standardized diagnostic criteria, common reagents, and database resources. a clinical diagnosis of canine ehrlichiosis may be based on the manifestation of fever, thrombocytopenia, anorexia, nasolacrimal discharge, epistaxis, and exclusion of autoimmune and common canine vector borne diseases. these parameters are not invariab ...200011193652
analysis of transcriptionally active gene clusters of major outer membrane protein multigene family in ehrlichia canis and e. chaffeensis.ehrlichia canis and e. chaffeensis are tick-borne obligatory intramonocytic ehrlichiae that cause febrile systemic illness in humans and dogs, respectively. the current study analyzed the pleomorphic multigene family encoding approximately 30-kda major outer membrane proteins (omps) of e. canis and e. chaffeensis. upstream from seca and downstream of hypothetical transcriptional regulator, 22 paralogs of the omp gene family were found to be tandemly arranged except for one or two genes with oppo ...200111254561
spirochetemia caused by borrelia turicatae infection in 3 dogs in texas.spirochetemia was diagnosed in 2 siberian huskies and a rottweiler from the northwestern region of texas between june 1999 and october 2001. clinical findings were nonspecific; tick exposure was documented in 2 of the dogs. hematologic abnormalities included anemia (n=2), neutrophilia (n=2, including 1 with a left shift), lymphopenia (n=3), eosinopenia (n=3), and thrombocytopenia (n=2). one anemic dog had a positive coombs' test. in 1 dog, western blot analysis of serum yielded multiple positive ...200717523100
sequence and phylogenetic analysis of the 16s rrna gene of ehrlichia canis strains in dogs with clinical monocytic ehrlichiosis.the purpose of this study was to characterize, at the molecular level, the ehrlichia canis strains involved in naturally occurring canine monocytic ehrlichiosis (cme) in greece, and to investigate if any sequence diversity exists between the 16s rrna genes of those involved in the mild non-myelosuppressive or the severe myelosuppressive form of cme. to this end, amplification of the ehrlichial 16s rrna gene was attempted by nested polymerase chain reaction (pcr) assays in bone marrow (bm) aspira ...200717624694
recombinant major antigenic protein 2 of ehrlichia canis: a potential diagnostic tool.the major antigenic protein 2 (map2) of ehrlichia canis was cloned and expressed. the recombinant protein was characterized and tested in an enzyme-linked immunosorbent assay (elisa) format for potential application in the serodiagnosis of canine monocytic ehrlichiosis. the recombinant protein, which contained a c-terminal polyhistidine tag, had a molecular mass of approximately 26 kda. the antigen was clearly identified by western immunoblotting using antihistidine antibody and immune serum fro ...200111427559
screening dogs in ontario for borrelia burgdorferi and ehrlichia canis should be selective rather than routine. 200717824154
serological survey for ehrlichia canis in urban dogs from the major population centres of northern australia.to detect evidence of ehrlichia canis infection of dogs from the major population centres of northern australia, if present.200111599818
doxycycline clearance of experimentally induced chronic ehrlichia canis infection in dogs.ineffective clearance of ehrlichia canis after doxycycline administration has been reported despite the fact that the recommended treatment for canine ehrlichiosis is doxycycline. the effectiveness of doxycycline in clearing e canis infection from the blood and tissues of dogs requires additional evaluation.200718196732
serosurvey of pathogens in domestic dogs on the border of noël kempff mercado national park, bolivia.the threat of disease transmission from domestic animals to wildlife has become recognized as an increasing concern within the wildlife community in recent years. domestic dogs pose a significant risk as reservoirs for infectious diseases, especially for wild canids. as part of a multifaceted ecologic study of maned wolves and other canids in the large, remote noël kempff mercado national park (nkmnp) in northeastern bolivia, 40 domestic dogs in two villages and at two smaller settlements border ...200818432094
molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in south africa.a total of 1,138 blood specimens were collected over a 6-year period (2000-2006) from domestic dogs in south africa. specimens from domestic dogs were obtained from the onderstepoort veterinary academic hospital (ovah) in pretoria, the society for the prevention of cruelty to animals (johannesburg, durban, east london and bloemfontein) and private practices from four provinces (gauteng, mpumalanga, kwazulu-natal and western cape). all specimens were screened for babesia, theileria, hepatozoon an ...200818502588
a study of cross-reactivity in serum samples from dogs positive for leishmania sp., babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test.to verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by indirect fluorescent antibody (ifat) and enzyme-linked immunosorbent assay (elisa). all serum samples from endemic areas were positive for leishmania sp., by elisa and ifat, 51% positive for babesia canis and 43% for ehrlichia canis by ...200818554433
factors associated with the occurrence of epistaxis in natural canine leishmaniasis (leishmania infantum).canine leishmaniasis (canl) is a common cause of epistaxis in dogs residing in endemic areas. the pathogenesis of canl-associated epistaxis has not been fully explored because of the limited number of cases reported so far.200818564224
serum hyperviscosity syndrome associated with ehrlichia canis infection in a dog.a 10-year-old male lhasa apso was examined because of pyrexia, vomiting, weight loss, lethargy, and disorientation. the clinical and laboratory findings were supportive of a diagnosis of serum hyperviscosity syndrome associated with an ehrlichia canis infection. after tetracycline therapy the serum hyperviscosity syndrome and e. canis infection were successfully resolved.198312002576
monitoring c-reactive protein in beagle dogs experimentally inoculated with ehrlichia canis.the concentrations of c-reactive proteins (crp) in the plasma of five beagle dogs experimentally inoculated with ehrlichia canis increased markedly. the concentrations began to increase between 4 and 16 days and peaked between 15 and 42 days after inoculation of e. canis. the peak concentrations ranged from 217.8 to 788.8 microg/ml (452.6 +/- 228.1 sd). after the peak, the concentrations of crp decreased rapidly. the pcr product of 16s rrna of e. canis became detectable in the five dogs between ...200212090289
diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes.although the diagnosis of canine leukemia and lymphoma in advanced stages is usually uncomplicated, some presentations of the disease can be a diagnostic challenge. in certain situations, lymphoma and leukemia can be difficult to distinguish from a benign reactive proliferation of lymphocytes. because clonality is the hallmark of malignancy, we have developed an assay that uses the polymerase chain reaction to amplify the variable regions of immunoglobulin genes and t-cell receptor genes to dete ...200312627711
retrospective clinical and molecular analysis of conditioned laboratory dogs (canis familiaris) with serologic reactions to ehrlichia canis, borrelia burgdorferi, and rickettsia rickettsii.dogs are susceptible to different tickborne infections, including members of the anaplasmataceae (ehrlichia canis, e. ewingii, e. chaffeensis, anaplasma phagocytophilum, a. platys), borrelia burgdorferi, and rickettsia rickettsii. these diseases can manifest with clinical signs including fever, anorexia, malaise, lameness, rash, and bleeding episodes; however, these signs are nonpathognomonic, and infections can occur in the absence of clinical signs. hematologic abnormalities can include leukop ...200818947166
canine inflammatory myopathy associated with leishmania infantum infection.inflammatory myopathy associated with several infectious diseases occurs in dogs including those caused by toxoplasma gondii, neospora caninum, ehrlichia canis and hepatozoon canis. however, muscle disease due to leishmania infection has been poorly documented. the aim of this study was to examine the distribution and types of cellular infiltrates and expression of mhc class i and ii in muscle biopsies obtained from 15 male beagle dogs from a breeder group with an established diagnosis of leishm ...200919084398
isolation of bartonella washoensis from a dog with mitral valve endocarditis.we report the first documented case of bartonella washoensis bacteremia in a dog with mitral valve endocarditis. b. washoensis was isolated in 1995 from a human patient with cardiac disease. the main reservoir species appears to be ground squirrels (spermophilus beecheyi) in the western united states. based on echocardiographic findings, a diagnosis of infective vegetative valvular mitral endocarditis was made in a spayed 12-year-old female doberman pinscher. a year prior to presentation, the re ...200314605197
a serosurvey of hepatozoon canis and ehrlichia canis antibodies in wild red foxes (vulpes vulpes) from israel.a seroepidemiological survey was conducted to investigate the prevalence of antibodies reactive with ehrlichia canis and hepatozoon canis antigens in free-ranging red foxes (vulpes vulpes) in israel. of 84 fox sera assayed, 36% were seropositive for e. canis by the indirect fluorescent antibody (ifa) test and 24% were positive for h. canis using an enzyme-linked immunosrbent assay (elisa). canine ehrlichiosis and hepatozoonosis appear to be endemic in the wild red fox populations in israel, and ...200415036573
chronic canine ehrlichiosis (ehrlichia canis): a retrospective study of 19 natural cases.nineteen dogs from greece with chronic ehrlichiosis were studied. the dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to ehrlichia canis (e. canis) antigens, and had no history of drug or radiation exposure. anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. all these animals eventually died, irrespective of the treatment applied. some dogs were also serolo ...200415131097
major species-specific antibody epitopes of the ehrlichia chaffeensis p120 and e. canis p140 orthologs in surface-exposed tandem repeat regions.ehrlichia chaffeensis and e. canis have a small subset of tandem repeat (tr)-containing protein orthologs, including p120/p140, which elicit strong antibody responses. the tr regions of these protein orthologs are immunoreactive, but the molecular characteristics of the p120/p140 epitopes have not been determined. in this study, the immunodeterminants of the e. chaffeensis p120 and e. canis p140 were identified and molecularly defined. major antibody epitope-containing regions of both p120 and p ...200919420187
rapid screening and cultivation of ehrlichia canis from refrigerated carrier blood. 200919438630
comparison of results for serologic testing and a polymerase chain reaction assay to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis.to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis and examine the correlation between results for an elisa, indirect immunofluorescent antibody (ifa) test, and polymerase chain reaction (pcr) assay.200415478765
occurrence of dirofilaria immitis and tick-borne infections caused by anaplasma phagocytophilum, borrelia burgdorferi sensu lato and ehrlichia canis in domestic dogs in france: results of a countrywide serologic survey.the occurrence of dirofilaria immitis antigen and antibodies against tick-borne pathogens in french dogs has been analysed based on 1,050 blood samples. serum samples of 919 dogs (group a) were sent for a variety of diagnostic investigations, further 131 dogs (group b) were tested for a tentative diagnosis of heartworm disease. all samples were tested for d. immitis antigen. samples in group a were also tested for specific antibodies against three tick-borne pathogens (anaplasma phagocytophilum, ...200919575231
free light-chain proteinuria and normal renal histopathology and function in 11 dogs exposed to lleishmania infantum, ehrlichia canis, and bbabesia canis.the purpose of this retrospective study was to investigate the relationship among proteinuria consisting of immunoglobulin free light chains (flcs), renal histopathologic findings, and routine markers of renal function in 11 dogs exposed to leishmania infantum (n = 8), ehrlichia canis (n = 2), and babesia canis (n = 1). flc proteinuria was suspected based on identification of a 22- to 27-kda band by sodium dodecyl sulfate-agarose gel electrophoresis (sds-age) and later confirmed by immunofixatio ...200415515575
babesia canis and other tick-borne infections in dogs in central poland.vector-borne infections constitute increasing health problem in dogs worldwide, including sled dogs, dramatically decreasing the fitness of working dogs and even leading to death. in the period 2006-2008 eighty-two blood samples were collected from eight sled dog kennels in central poland. the prevalence of four vector-borne infections (babesia canis, bartonella sp., anaplasma/ehrlichia and borrelia burgdorferi) was estimated in 82 sled dogs using pcr and nested pcr for diagnosis and the same me ...200919837515
acquired amegakaryocytic thrombocytopenia--four cases and a literature review.acquired amegakaryocytic thrombocytopenla was diagnosed in four dogs. initial platelet counts in all four dogs were less than 50,000 x 10(9)/litre and initial bone marrow examinations revealed megakaryocytic hypoplasia with minimal changes in the erythroid and myeloid cell lines. two dogs had evidence of idiopathic immune-mediated disease and two dogs had evidence of associated infectious disease. one dog had a positive antibody titre to borrella burgdorferi, and one dog had positive titres to b ...200415515801
detection of babesia canis subspecies and other arthropod-borne diseases in dogs from tirana, albania.summary. the importance of arthropod-borne diseases increased in the recent past in particular due to frequent travel with dogs in or by importing of dogs from regions with endemic occurrence of these diseases. while the epidemiological situation is well known for the western parts of the mediterranean, only limited data is available for eastern europe and the balkans. thirty clinically healthy dogs from suburban areas of tirana, albania, were tested for babesia canis, hepatozoon spp., leishmani ...200919915816
[ehrlichia and babesia infections in dogs in the netherlands].a retrospective study was performed at the department of clinical sciences of companion animals at utrecht university amongst 75 dogs diagnosed with a babesia canis and/or an ehrlichia canis infection. the majority of the dogs had visited an endemic area (most often the mediterranean area or the dutch antilles), but two dogs became infected with babesia in the netherlands. babesia infections were associated with a stay in an endemic area and an incubation period that are both significantly short ...200415622893
comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in korea.abstract: a serological survey for dirofilaria immitis, anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi infections in rural hunting and urban shelter dogs mainly from southwestern regions of the republic of korea (south korea) was conducted. from a total of 229 wild boar or pheasant hunting dogs, the number of serologically positive dogs for any of the four pathogens was 93 (40.6%). the highest prevalence observed was d. immitis (22.3%), followed by a. phagocytophilum (18.8% ...201020377869
imported and travelling dogs as carriers of canine vector-borne pathogens in germany.abstract:201020377872
ocular signs of canine monocytic ehrlichiosis: a retrospective study in dogs from barcelona, spain.canine monocytic ehrlichiosis (cme) is a tick-borne disease caused by the rickettsia ehrlichia canis. ocular lesions are a common feature of the disease and can be present in all stages. the purpose of this retrospective study was to determine the prevalence, type and response to treatment of ocular lesions associated with monocytic ehrlichiosis in 46 affected dogs presented to the autonomous university of barcelona-veterinary teaching hospital (uab-vth) from january 2000 to december 2002. dogs ...200516359361
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