genotyping of campylobacter spp. from retail meats by pulsed-field gel electrophoresis and determine the genetic relatedness of campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed-field gel electrophoresis (pfge) and automatic ribotyping.200616405698
a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology.200415375107
campylobacter prevalence in lactating dairy cows in the united states.the objective of the present study was to determine the prevalence of intestinal campylobacter in lactating dairy cows from various regions of the united states. participating commercial dairy farms were chosen at random and were part of a national survey to determine e. coli o157:h7 and salmonella prevalence in dairy cows. farms had no previous history of campylobacter problems. fecal samples were collected rectally from 720 cows on farms in the northeast (four farms), in the desert southwest ( ...200415270504
diffusion of thermophilic campylobacter in the pesaro-urbino area (italy) from 1985 to 1992.the results of research on the spreading of campylobacter in the pesaro-urbino area carried out from 1985 to 1992 are presented. materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and 632 of various types of food. two hundred and nine strains of campylobacter were isolated (9%), most of which were campylobacter jejuni (80%), with particular frequency in food products (chicken carcas ...19957489778
occurrence of campylobacter in retail foods in ireland.a surveillance study was carried out to determine the prevalence of campylobacter in a range of retail foods purchased in three irish cities over a 20-month period between march 2001 and october 2002. in total 2391 food samples were analysed during this period. campylobacter was isolated from 444 raw chicken (49.9%), 33 turkey (37.5%) and 11 duck samples (45.8%). lower isolation rates of 7/221 (3.2%), 10/197 (5.1%) and 31/262 (11.8%) were observed for raw beef, pork and lamb, respectively. one s ...200415282123
experimental infection of the bovine udder with campylobacter coli/jejuni.five quarters of the udders of two lactating cows were infected by intramammary inoculation with campylobacter coli/jejuni in doses ranging from 2.6 colony-forming units (c.f.u.) to 3.8 x 10(9) c.f.u. the infected quarters developed clinical mastitis and the campylobacters were reisolated in large numbers from the milk. the milk from the uninfected quarters, and blood and faeces remained free of the organisms. the campylobacters could only be isolated by incubation of culture plates in a microae ...19807052224
further characterization of campylobacter isolated from u.s. dairy cows.the objective of the present study was to compare polymerase chain reaction (pcr) identification with ribotype results and to use pulsed field electrophoresis (pfge) to correlate genotypic patterns with antibiotic resistance of campylobacter isolated from lactating dairy cows in the united states. thirty isolates were studied. twenty-seven of the isolates were identified by pcr as campylobacter jejuni and three were identified as campylobacter coli. genotypic patterns of 15 isolates were determi ...200515992313
characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004.we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ...200617085893
[some differences on biological characteristics of campylobacter jejuni].fecal samples from 195 diarrheic patients in different age, rectal and cloacal swabs from 656 normal and diarrheic animals and poultry, 108 visceral materials from the dead animals with diarrhoea were cultured for compylobacter jejuni. a total of 458 strains of campylobacters (445 strains of c. jejuni, 13 strains of c. coli) were isolated and identified, and some biological characteristics of these strains were observed. lior's biotyping scheme was used for biotyping 354 strains of campylobacter ...200012548753
fecal shedding of campylobacter and arcobacter spp. in dairy cattle.campylobacter jejuni, campylobacter coli, and arcobacter spp. were detected in feces of healthy dairy cows by highly specific multiplex-pcr assays. for c. jejuni, at this one-time sampling, cows from 80.6% of farm operations (n = 31) and 37.7% of individual dairy cattle fecal samples (n = 2,085) were positive. farm management factors were correlated with prevalence in herds in which >25% of cows were positive for c. jejuni. statistical significance was set at a p of 0.20. using these criteria, a ...200010788372
prevalence and strain diversity of thermophilic campylobacters in cattle, sheep and swine determine prevalence and strain diversity of thermophilic campylobacters in healthy ruminants and swine.200717897201
anti-campylobacter, anti-aerobic, and anti-oxidative effects of roselle calyx extract and protocatechuic acid in ground beef.the inhibitory effect of roselle calyx extract and protocatechuic acid against susceptible and antibiotic-resistant campylobacter jejuni, c. coli and c. fetus in agar plate and ground beef was examined. the minimal inhibitory concentrations of roselle calyx extract and protocatechuic acid against susceptible and antibiotic-resistant campylobacter species were in the range of 96-152 and 20-44 microg/ml, respectively. temperature treatments from 25 to 100 degrees c did not affect the anti-campylob ...200818620770
high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance.campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ...19957561196
molecular characterization and environmental mapping of campylobacter isolates in a subset of intensive poultry flocks in breeder and intensive broiler flocks together with the corresponding poultry farm environment were sampled for the presence of campylobacter with the aim of identifying potential sources and transmission routes of poultry flock contamination. the genetic diversity of a subset of campylobacter isolates was examined by analysis of the flaa-short variable region (svr). additional discrimination for a further subset of these isolates was achieved using multilocus sequence typing (mlst) analysi ...201021039133
development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari.contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ...19968914757
risk factors for campylobacter jejuni and campylobacter coli in young cattle on english and welsh farms.campylobacter jejuni and c. coli are the most prevalent causes of bacterial diarrhoea in most of the western world. in great britain, the source remains unknown for the majority of cases, though poultry is considered the main source of infection. molecular typing methods identify cattle as a potential source of a proportion of the non-source-attributed cases, mainly through direct contact, environmental contamination or milk, but little is known about the epidemiology of campylobacter in cattle. ...200918835052
detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products.the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ...200617010041
campylobacter in food animals and humans in northern thailand.cross-sectional, longitudinal, and case-control studies were conducted to describe the epidemiology of campylobacter in chickens, swine, dairy cows, farm workers, nonfarm residents, and children with diarrhea. samples were collected in chiang mai and lamphung provinces of northern thailand from 2000 through 2003. a total of 2,360 samples were processed. results from the cross-sectional study indicated that the prevalences of campylobacter in chickens at the farm, slaughterhouse, and market were ...200516355821
genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ...200516204541
evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections.campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates.200717220252
dissemination of antimicrobial resistant strains of campylobacter coli and campylobacter jejuni among cattle in washington state and california.the purpose of this study was to investigate the genetic similarity of campylobacter jejuni and campylobacter coli with similar antimicrobial resistance phenotypes, isolated from cattle on different farms and at different times, in order to evaluate the possible existence of disseminated antimicrobial resistant clones. pfge after smai and kpni restriction identified 23 and 16 distinct pfge patterns among 29 c. jejuni and 66 c. coli isolates, respectively. in c. coli, 51 (77%) of the resistant is ...200717321701
prevalence of campylobacter spp. in cattle in finland and antimicrobial susceptibilities of bovine campylobacter jejuni strains.the study investigated the prevalence of campylobacter spp. in finnish cattle at slaughter and carcass contamination after slaughter. during the period january to december 2003, bovine rectal fecal samples (n=952) and carcass surface samples (n=948) from 12 out of 15 finnish slaughterhouses were examined. in total, campylobacters were detected in 31.1% of fecal samples and in 3.5% of carcass surface samples. campylobacter jejuni was isolated from 19.5%, campylobacter coli from 2.2%, and presumpt ...200717369335
formation of cytotoxins by enteric campylobacter in humans and animals.campylobacter (c.) jejuni from persons suffering from diarrhoea, from organs of poultry, c. jejuni and c. fetus ssp. fetus from the gastrointestinal tract of calves and adult cattle as well as a number of reference strains were examined for cytotoxin formation in a cho-k1 cell culture test. during evaluation, three morphologically different pictures were observed. the first cytotoxin caused a formation of strikingly large, rounded or polymorphic and elongated cells which was associated with redu ...19989809404
a survey of campylobacter species shed in faeces of beef cattle using polymerase chain reaction.a polymerase chain reaction (pcr)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. to ensure the removal of pcr inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control dna (i.e., dna designed to amplify with the campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. in only two samples (0.5%) were the internal control or campylobacter genu ...200314735214
cecal colonization of chicks by bovine-derived strains of campylobacter.campylobacter jejuni and campylobacter coli strains were isolated from feces of dairy cattle at farms with no known problem due to campylobacteria. farms were located in the northeast, desert southwest, and pacific west. twenty isolates were identified by ribotyping with a riboprinter. the ability of these bovine isolates to colonize the ceca of chicks was determined by challenge inoculation and reisolation of the challenge strain from the ceca at 1 and 2 wk after challenge. isolates recovered f ...200314708992
rapid and specific enzyme immunoassay on hydrophobic grid membrane filter for detection and enumeration of thermophilic campylobacter spp. from milk and chicken rinses.six commercially available anti-campylobacter antibodies were examined for their applicability in an enzyme immunoassay on hydrophobic grid membrane filters, both for the detection and enumeration of campylobacter spp. when a panel of nine campylobacter (seven campylobacter jejuni and two campylobacter coli) and eight non-campylobacter strains were used in a dot-blot format enzyme immunoassay to test the specificity of these antibodies, only one polyclonal antibody (biodesign) detected all campy ...200010772214
characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens.phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ...200415574903
a national investigation of the prevalence and diversity of thermophilic campylobacter species in agricultural watersheds in canada.the occurrence and diversity of thermophilic campylobacter species (c. jejuni, coli, and lari) were studied in water samples from four river basins located across canada. these basins located in quebec (bras d'henri), alberta (oldman), ontario (south nation), and british columbia (sumas) represented some of the most intensive farming areas in canada for hog, beef cattle, dairy cattle, and poultry, respectively. this study analyzed 769 water samples collected from 23 monitoring sites with agricul ...201424930011
prevalence and genetic diversity of campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area.water samples were taken systematically from a 100-km2 area of mainly dairy farmland in northwestern england and examined for campylobacter spp. pulsed-field gel electrophoresis-restriction fragment length polymorphism (pfge-rflp) and flaa strain typing of campylobacter jejuni and campylobacter coli isolates were done. data on the water source and the adjacent environment were recorded and examined as explanatory variables. campylobacter spp. were isolated from 40.5% (n = 119) of the water sampl ...200515812015
prevalence and antimicrobial resistance of campylobacter in us dairy obtain an overview of the prevalence and antimicrobial resistance of campylobacter in faeces of us dairy cows in 2002.200717578422
[molecular investigation of relatedness of campylobacter coli isolated from child with campylobacteriosis and healthy, household dog].thermotolerant species of campylobacter (mainly c. jejuni and c. coli) are among the most frequently isolated bacterial agents of gastroenteritis in many developed countries. c. coli is less prevalent than c. jejuni. the main reservoir of c. coli is swine, however this pathogen may also be found in poultry, cattle and pets. genotypic methods have successfully been applied to the subtyping of campylobacter species in epidemiological investigation. in the present study we report a case of isolatio ...200920120926
antimicrobial susceptibility patterns of thermophilic campylobacter spp. from humans, pigs, cattle, and broilers in denmark.the mics of 16 antimicrobial agents were determined for 202 campylobacter jejuni isolates, 123 campylobacter coli isolates, and 6 campylobacter lari isolates from humans and food animals in denmark. the c. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the c. coli isolates originated from humans (7), broilers (17), and pigs (99); and the c. lari isolates originated from broilers (5) and cattle (1). all isolates were susceptible to apramycin, neomycin, and ...19979333055
isolation and characterization of cephalothin-susceptible campylobacter coli from slaughter a recent meat survey, 10 of 13 (77%) campylobacter coli isolates were susceptible to cephalothin. these organisms were isolated from nine slaughter cattle from eight meat packing establishments. all 10 isolates grew at 43 degrees c but not at 25 degrees c, were catalase and oxidase positive, and were susceptible to nalidixic acid (30 micrograms) and cephalothin (30 micrograms). the cultures were subsequently identified as c. coli serogroup 20, biotype i (lior scheme). sodium dodecyl sulfate-p ...19863771747
pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ...19836650962
occurrence of campylobacter jejuni and campylobacter coli and their biotypes in beef and dairy cattle from the south of chile.the prevalence of campylobacter jejuni and campylobacter coli and their biotypes in beef and dairy cattle from the south of chile was established. campylobacter were statistically more prevalent among beef cattle (35.9%) than among dairy cattle (21.3%), being c. jejuni the species most frequently isolated.200924031386
pcr detection of seven virulence and toxin genes of campylobacter jejuni and campylobacter coli isolates from danish pigs and cattle and cytolethal distending toxin production of the study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (cdt) production of campylobacter jejuni and c. coli isolates from danish pigs and cattle.200312752808
spatiotemporal homogeneity of campylobacter subtypes from cattle and sheep across northeastern and southwestern scotland.source attribution using molecular subtypes has implicated cattle and sheep as sources of human campylobacter infection. whether the campylobacter subtypes associated with cattle and sheep vary spatiotemporally remains poorly known, especially at national levels. here we describe spatiotemporal patterns of prevalence, bacterial enumeration, and subtype composition in campylobacter isolates from cattle and sheep feces from northeastern (63 farms, 414 samples) and southwestern (71 farms, 449 sampl ...200919700557
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from cattle between 2002 and 2006 in france.feces from 2,255 cattle (calves, young beef cattle, and culled cows) were collected at slaughter from nine departments across france. campylobacter was recovered from 16.5% of the 2,255 samples (c. jejuni from 12.8% and c. coli from 3.7%), predominantly from calves. antimicrobial resistance to six antibiotics of medical and/or veterinary interest was tested with the e-test. resistance to tetracycline was found in most isolates (52.8% of c. jejuni isolates and 88.1% of c. coli isolates) in contra ...201020501032
risk of escherichia coli o157:h7, non-o157 shiga toxin-producing escherichia coli, and campylobacter spp. in food animals and their products in qatar.escherichia coli o157:h7, non-o157 e. coli, and campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. the associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bac ...201526408129
campylobacter genotyping to determine the source of human infection.campylobacter species cause a high proportion of bacterial gastroenteritis cases and are a significant burden on health care systems and economies worldwide; however, the relative contributions of the various possible sources of infection in humans are unclear.200919275496
[the antigenic structure of vibrions studied by means of agglutination and fluorescent antibodies].cross immunofluorescent and serological studies were made of strains of vibrio coli of different forms-a short, commashaped strain and other long, spiral-shaped and serpent-like strains isolated from swine. some standard strains of vibrio foetus were also included for comparison. an antigenic difference was established between vibrions of long and short forms, as well as the known difference between strains isolated from swine and those from cattle which can be established by means of immunofluo ...1975779698
prevalence and antimicrobial resistance of campylobacter species isolated from raw camel, beef, lamb, and goat meat in iran.campylobacter spp. are one of the most common causes of acute bacterial gastroenteritis in human beings which are transmitted mostly via food originating from animals. this study was conducted to determine the prevalence and antimicrobial resistance of campylobacter spp. isolated from retail raw meats in iran. from june 2008 to june 2009, a total of 722 raw meat samples from camel (n = 107), beef (n = 190), lamb (n = 225), and goat (n = 180) were purchased from randomly selected retail outlets i ...201020184450
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