detection and identification of leptospira interrogans serovars by pcr coupled with restriction endonuclease analysis of amplified dna.primers for pcr were selected from a sequenced fragment of clone pl590, which contains a repetitive element present in the genome of leptospira interrogans serovar hardjo type hardjoprajitno (m. l. pacciarini, m. l. savio, s. tagliabue, and c. rossi, j. clin. microbiol. 30:1243-1249, 1992). a specific dna fragment was amplified from the genomic dnas of serovar hardjo type hardjoprajitno and nine serovars also belonging to l. interrogans as a consequence of the spread of the same or a closely rel ...19948027346
magnetic immuno capture pcr assay (mipa): detection of leptospira borgpetersenii serovar hardjo.magnetic immuno pcr assay (mipa) was developed for the rapid detection of leptospires excreted in urine samples (n = 59) collected from 35 experimentally infected cattle. the immunomagnetic separation of leptospires from inhibitors in frozen formalin fixed bovine urine prior to pcr detection resulted in a marked improvement on previous detection methods. mipa is a rapid 5 step protocol requiring 70 mins preparation time prior to amplification, which consistently detects 10(1) organisms. mipa det ...19979228689
amoxycillin as an alternative to dihydrostreptomycin sulphate for treating cattle infected with leptospira borgpetersenii serovar assess the effect of amoxycillin treatment on urinary excretion of leptospires from cattle infected with leptospira borgpetersenii serovar hardjo.19979404617
experimental leptospira borgpetersenii serovar hardjo infection of pregnant observe the effect upon the foetus of experimental infection of pregnant cattle with leptospira borgpetersenii serovar hardjo.19979404618
development of an immunomagnetic antigen capture system for detecting leptospires in bovine urine.a magnetic bead antigen capture system which combined the use of two evolving techniques - immunomagnetic separation (ims) and time-resolved fluoroimmunoassay (tr-fia) - was developed to detect leptospira borgpetersenii serovar hardjo in bovine urine. the assay utilised monoclonal antibody coated magnetic beads to capture leptospiral antigen which was in turn detected using another monoclonal antibody (indicator) labelled with biotin. signal was generated by the binding of europium labelled stre ...19989625467
sanitary status of oocytes and embryos collected from heifers experimentally exposed to leptospira borgpetersenii serovar a preliminary trial and three experiments, a total of 30 holstein heifers were experimentally infected with a culture of leptospira borgpetersenii serovar hardjobovis via one or more routes (uterine, cervical supraconjunctival, intranasal) and oviductal and uterine fluids recovered post-mortem or in vivo following superovulation with fsh. all routes of administration were effective in establishing leptospira infection in the reproductive tract and leptospira were identified in the oviductal a ...19989877053
comparison of polymerase chain reaction assays with bacteriologic culture, immunofluorescence, and nucleic acid hybridization for detection of leptospira borgpetersenii serovar hardjo in urine of compare sensitivity and specificity of various polymerase chain reaction (pcr) assays for detection of leptospira borgpetersenii serovar hardjo in bovine urine and to compare results of the optimal pcr assay with results of immunofluorescence, nucleic acid hybridization, and bacteriologic culture.200010714525
evaluation of antibiotics for treatment of cattle infected with leptospira borgpetersenii serovar evaluate antibiotics for treatment of cattle with leptospirosis caused by leptospira borgpetersenii serovar hardjo.200111549093
comparison of fluorescent antibody and microscopic agglutination testing for leptospira in pregnant and nonpregnant cows.serum and urine samples from 30 cows (15 pregnant and 15 nonpregnant) from each of 10 georgia dairy herds (total cows = 300) were examined by microscopic agglutination testing (mat) and direct fluorescent antibody testing (fat), respectively. seven of the 10 herds had at least 1 cow with a positive fat, and all of the herds had at least 1 cow with a reciprocal mat titer > or =100 for 1 or more serovars. serological testing was not helpful in identifying the infecting serovar for cows with a posi ...201020093682
a leptospira borgpetersenii serovar hardjo vaccine induces a th1 response, activates nk cells, and reduces renal colonization.chronic infection of cattle with leptospira borgpetersenii serovar hardjo reduces animal production through reproductive failure and presents a persistent health threat to workers in the animal industry. cattle are maintenance hosts for serovar hardjo, and development of vaccines that establish long-term protective immunity has been problematic; induction of high titers of anti-serovar hardjo antibody does not appear to be protective. rather, development of an antigen-specific th1 response appea ...201121288995
development of chronic and acute golden syrian hamster infection models with leptospira borgpetersenii serovar hardjo.the golden syrian hamster (mesocricetus auratus) is frequently used as a model to study virulence for several leptospira species. onset of an acute lethal infection following inoculation with several pathogenic leptospira species has been widely adopted for pathogenesis studies. an important exception is the outcome following inoculation of hamsters with live l. borgpetersenii serovar hardjo, the primary cause of bovine leptospirosis and a cause of human infections. typically, inoculation of ham ...201121670193
effect of a monovalent vaccine against leptospira borgpetersenii serovar hardjo strain hardjobovis on fertility in holstein dairy determine whether vaccination with a monovalent vaccine against leptospira borgpetersenii serovar hardjo strain hardjobovis would improve reproductive efficiency in holstein cattle in a commercial dairy setting.201323683023
application of an integrated outbreak management plan for the control of leptospirosis in dairy cattle herds.two outbreaks of leptospira borgpetersenii serovar hardjo infection in dairy cattle herds were managed through the application of enhanced biosecurity measures, whole-herd antibiotic treatment and vaccination. micro-agglutination test antibody titres were determined in paired serum samples at 3 weeks (t1: n = 125, 97% seropositivity, median 800, range 100-12 800) and 24 weeks (t2: n = 110, 88% seropositivity, median 200, range 100-6400) after vaccination and studied in relation to cows' age, her ...201423920354
a cluster of three cases of leptospirosis in dairy farm workers in new zealand.we report a cluster of three cases of leptospirosis on a new zealand dairy farm, with regard to clinical, laboratory, and environmental findings. the cluster is discussed against the annual incidence of leptospirosis in humans and cattle, and the vaccination of cattle as one means of preventing human cases on farms.201424481382
isolation and molecular characterization of leptospira borgpetersenii serovar hardjo strain hardjobovis in the urine of naturally infected cattle in brazil.most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar hardjo, which is widespread and considered to be the most adapted to bovine hosts. however, using only serological studies is not sufficient to identify and distinguish species of leptospires. the aim of this study was report the first isolation in brazil of two strains serovar hardjo obtained in urine samples from naturally infected cows in a sma ...201626909976
a cross sectional observational study to estimate herd level risk factors for leptospira spp. serovars in small holder dairy cattle farms in southern chile.the south of chile constitutes the main cattle milk producing area of the country. regarding leptospirosis control in chile, there is neither an official program nor an epidemiological characterization of smallholder dairy farms. this study was carried out to determine leptospira seroprevalence and to evaluate risk factors associated with seropositivity at herd level in smallholder bovine dairy herds in southern chile.a cross-sectional study was conducted, and a convenient sample of 1,537 appare ...201424906684
seroprevalence of leptospira borgpetersenii serovar javanica infection among dairy cattle, rats and humans in the cauvery river valley of southern india.leptospirosis is a major problem of dairy farms in tamilnadu, india, resulting in abortions, stillbirths and infertility. serologic and genetic analyses of samples from cattle, humans and rodents were performed in order to estimate infection prevalence and identify leptospiral species. five hundred and fifteen sera and 76 urine samples were collected from dairy cattle on 25 farms including a farm that practiced rat control. sera and kidney samples were also collected from field rats (rattus norv ...201121706947
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