| trichinella spiralis (t1) and trichinella t5: a comparison using animal infectivity and molecular biology techniques. | we compared trichinella t5 of bobcat (lynx rufus) origin with trichinella spiralis (t1) by using animal infectivity and molecular biology techniques. swine, sd rats, and cf1 mice were highly resistant to infection with trichinella t5 but sensitive to t. spiralis, whereas deer mice (peromyscus maniculatus) had similar sensitivity to both parasites. the fecundity of trichinella t5 in deer mice was 10-35-fold higher in comparison to the fecundity in laboratory rodents (sd rats and cf1 mice). fecund ... | 1997 | 9057702 |
| trichinella infection in wildlife of the southwestern united states. | several potential mammalian reservoirs of sylvatic species of trichinella were examined from texas, new mexico, and arizona. during 1998-99, tongues were collected from a black bear (ursus americanus) in arizona; from 9 black bears, a coyote (canis latrans), and a mountain lion (felis concolor) in new mexico; and from 154 coyotes, 32 raccoons (procyon lotor), 13 opossums (didelphis marsupialis), 4 ocelots (leopardus pardalis), 3 bobcats (lynx rufus), and 5 feral hogs (sus scrofa) in southern tex ... | 2001 | 11695403 |
| human dispersal of trichinella spiralis in domesticated pigs. | to investigate the human impact on the evolutionary ecology of animal pathogens, we compared genetic diversity of severe foodborne parasites contracted by eating infected pork or wild game. in particular, we characterized trichinella spp. from twenty-eight countries and four continents by genotyping nine microsatellite loci and sequencing one-fifth of the mitochondrial genome. all specimens of trichinella spiralis, a swine parasite that can infect many species of wildlife, were remarkably unifor ... | 2008 | 18718558 |
| next-generation sequencing of the trichinella murrelli mitochondrial genome allows comprehensive comparison of its divergence from the principal agent of human trichinellosis, trichinella spiralis. | the mitochondrial genome's non-recombinant mode of inheritance and relatively rapid rate of evolution has promoted its use as a marker for studying the biogeographic history and evolutionary interrelationships among many metazoan species. a modest portion of the mitochondrial genome has been defined for 12 species and genotypes of parasites in the genus trichinella, but its adequacy in representing the mitochondrial genome as a whole remains unclear, as the complete coding sequence has been char ... | 2010 | 20946970 |
| survival of north american genotypes of trichinella in frozen pork. | north american genotypes of trichinella spiralis (t-1), trichinella nativa (t-2), trichinella pseudospiralis (t-4), trichinella murrelli (t-5), and trichinella t-6 were examined for susceptibility to freezing in pork using time-temperature combinations that have been proven to inactivate t. spiralis. infections were established in 3-month-old pigs of mixed sex and breed by oral inoculation of 10,000 muscle larvae (ml) (all genotypes, rodent-derived ml), 20,000 ml (t-1, t-4, and t-5; cat-derived ... | 2009 | 20003740 |
| the first occurrence of trichinella murrelli in wild boar in iran and a review of iranian trichinellosis. | trichinella larvae isolated from the thigh muscle of a wild boar, sus scrofa, captured from gilan province, northern iran, was processed for dna analysis. polymerase chain reaction (pcr) for amplification of the 5s rdna fragment demonstrated a 700 bp band on agarose gel. analysis of dna sequencing by blast confirmed the isolate as t. murrelli. this report constitutes the first recorded occurrence of t. murrelli in asia, and also the first occurrence in a wild boar host. | 2009 | 19531274 |
| molecular characterization of trichinella genotypes by inter-simple sequence repeat polymerase chain reaction (issr-pcr). | a bulk analysis of inter-simple sequence repeat-polymerase chain reaction (issr-pcr) provides a quick, reliable, and highly informative system for dna banding patterns that permit species identification. the present study evaluates the applicability of this system to trichinella species identification. after a single amplification carried out on a single larva with the primer 816([ca]nry) under high stringency conditions, which provide high reproducibility, we were able to identify by consistent ... | 2006 | 16884006 |
| new patterns of trichinella infection. | human and animal trichinellosis should be considered as both an emerging and reemerging disease. the reemergence of the domestic cycle has been due to an increased prevalence of trichinella spiralis, which has been primarily related to a breakdown of government veterinary services and state farms (e.g., in countries of the former ussr, bulgaria, romania), economic problems and war (e.g., in countries of the former yugoslavia), resulting in a sharp increase in the occurrence of this infection in ... | 2001 | 11516583 |
| sylvatic trichinellosis in texas. | there are no published reports of domestic or sylvatic trichinellosis in texas. the aim of the present survey was to determine the presence of trichinella species in selected representative species of potential wildlife reservoirs in southern texas. in 1998-99, tongues of 211 wild mammals were collected in southern texas: 154 coyotes (canis latrans), three bobcats (lynx rufus), 32 racoons (procyon lotor), 13 opossum (didelphis marsupialis), four ocelots (leopardus pardalis) and five wild boars ( ... | 2001 | 11484391 |
| sylvatic and domestic trichinella spp. in wild boars; infectivity, muscle larvae distribution, and antibody response. | thirty-six wild boars were inoculated with trichinella spiralis, trichinella nativa, trichinella britovi, trichinella pseudospiralis (ussr), t. pseudospiralis (usa), t. pseudospiralis (aust), trichinella murrelli, trichinella t6, and trichinella nelsoni. the wild boars were killed at 5 and 10 wk postinoculation (pi), and the number of muscle larvae per g (lpg) of tissue was determined for 18 muscles or muscle groups. five weeks pi, all trichinella genotypes had established as muscle larvae, but ... | 2001 | 11318560 |
| trichinella murrelli n. sp: etiological agent of sylvatic trichinellosis in temperate areas of north america. | trichinella t5, collected from sylvatic carnivores in north america, was identified previously as a different phenotype of trichinella, with an uncertain taxonomic level due to the availability of only 2 isolates. cross-breeding experiments carried out with single female and male larvae of 2 strains of trichinella t5, with single female and male larvae of 2 strains of trichinella spiralis, trichinella nativa, trichinella britovi, trichinella pseudospiralis, trichinella nelsoni, and trichinella t ... | 2000 | 10701576 |
| incorrect sequencing and taxon misidentification: an example in the trichinella genus. | molecular analyses such as polymerase chain reaction (pcr) and sequencing are very useful for taxon identification, especially when morphological characters useful for identifying taxa are lacking. however, the use of molecular tools can be the source of taxon misidentification if they are not correctly applied and the results are not critically evaluated and compared with the literature and genbank data. we describe a case of misidentification of a taxon of the genus trichinella due to sequenci ... | 2010 | 20230655 |
| infectivity, persistence, and antibody response to domestic and sylvatic trichinella spp. in experimentally infected pigs. | groups of pigs were inoculated with genotypes of trichinella belonging to: trichinella spiralis, trichinella nativa, trichinella britovi, trichinella pseudospiralis (from caucasus), t. pseudospiralis (from usa), trichinella murrelli, trichinella sp. (from north america), and trichinella nelsoni. the pigs were sacrificed between 5 and 40weeks p.i., and the number of muscle larvae per gram (l.p.g.) of tissue was determined as an average of 18 muscles. all trichinella genotypes were infective for p ... | 2000 | 10704604 |
| a complementary dna encoding an antigen from trichinella spiralis muscle larvae and its analog from trichinella t5 of bobcat origin: sequence, cloning and expressions. | reverse transcription-polymerase chain reaction (rt-pcr) was employed to amplify a cdna encoding an excretory-secretory (es) antigen with mol. wt 45-50 kda by sds-page from t. spiralis muscle larvae. the pcr product was purified by electrophoresis and sequenced by thermal cycle sequencing with primer walking. the cdna is 890 bp long and encodes a polypeptide of 255 amino acid (aa) residues. using the same methods, we also recovered a corresponding cdna from trichinella t5, which is 891 bp long a ... | 1997 | 9184935 |