| [demonstration of enterotoxin-forming pseudomonas aeruginosa strains in the intestinal ligature test in piglets]. | | 1979 | 119390 |
| [observations on antibacterial activity of thiamphenicol glycinate acetylcysteinate (author's transl)]. | in vitro thiamphenicol antibacterial activity in the presence of n-acetylcysteine as sodium salt proved to be enhanced when compared with that of the antibacterial alone against both gram-positive and gram-negative bacterial strains. the in vitro results were confirmed in vivo in the guinea-pig, where the sera from animals previously treated by i.m. route with thiamphenicol glycinate acetylcysteinate, showed a higher antibacterial activity than sera from animals treated with thiamphenicol glycin ... | 1979 | 122158 |
| antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (hr 111v), a new broad-spectrum cephalosporin. | cefquinome is a new injectable aminothiazolyl cephalosporin derivative. it is stable against chromosomally and plasmid-encoded beta-lactamases and has a broad antibacterial spectrum. staphylococcus aureus, streptococci, pseudomonas aeruginosa, and members of the family enterobacteriaceae (escherichia coli, salmonella spp., klebsiella spp., enterobacter spp., citrobacter spp., and serratia marcescens) are inhibited at low concentrations. cefquinome is also active against many strains of methicill ... | 1991 | 2014969 |
| isolation, structure, and immunogenicity of pseudomonas aeruginosa immunotype 4 high-molecular-weight polysaccharide. | a high-molecular-weight, immunogenic form of the lipopolysaccharide o side chain of pseudomonas aeruginosa fisher immunotype 4 (type 4, international antigenic typing system 1, lanyi o:6) was isolated and characterized. analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this high-molecular-weight polysaccharide and the type 4 o side chain. the polysaccharide was immunogenic in rabbits and mice, eliciting opsonophagocytic killing antibodies. immunization wi ... | 1989 | 2492260 |
| elastolytic activity of pseudomonas aeruginosa elastase. | elastolysis of insoluble elastin by pseudomonas aeruginosa elastase was found to be less specific (higher apparent km value) but more active (higher activity) than with pancreatic elastase. furthermore, pancreatic and p. aeruginosa elastases act synergistically during the initial stages of elastolysis. after extensive hydrolysis, the size distribution of digestion products was lower with p. aeruginosa than with pancreatic elastase. the higher extent of hydrolysis may be explained by the fact tha ... | 1989 | 2495818 |
| the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes. | the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ... | 1987 | 2822126 |
| sc-39026, a specific human neutrophil elastase inhibitor. | sc-39026, (+/-) 2-chloro-4-(1-hydroxyoctadecyl)benzoic acid, inhibits human neutrophil elastase with an ic50 of 0.5 microm (ki of 1.5 microm). its inhibition of elastase is reversible and noncompetitive at low concentrations (0.5-1.25 microm). inhibition is "mixed" at higher inhibitor concentrations. sc-39026 is inactive against hog pancreatic elastase, bovine alpha-chymotrypsin and pseudomonas aeruginosa elastase, but does inhibit human neutrophil cathepsin g with an ic50 of approximately 2.5 m ... | 1987 | 3115265 |
| [cell-free pseudomonas vaccine: its immunochemical characteristics and immunogenicity]. | two variants of cell-free protein vaccine have been prepared from the mixture of 4 p. aeruginosa strains, serovars 02, 06, 07 and 011, and from a single p. aeruginosa strain, serovar 02. the preparation contains proteins with molecular weight ranging from 20,000 to 100,000 and the admixture of lipopolysaccharide in negligible amounts (not exceeding 0.08% of dry weight). the vaccine produces no signs of toxicosis in laboratory animals. the vaccine effectively protects mice challenged with p. aeru ... | 1987 | 3124416 |
| effects of five antibiotics on adhesion and haemagglutinating properties of pseudomonas aeruginosa isolated from cystic fibrosis patients. | the aim of this study was to evaluate the in vitro effect of five antibiotics at sub-inhibitory concentrations on the adhesive and haemagglutinating properties of pseudomonas aeruginosa isolated from cystic fibrosis sputa. eleven isolates (mucoid and non-mucoid) from cystic fibrosis, and four isolates (mucoid and non-mucoid) from other chronic respiratory infections were tested. the adhesion test was performed on human lymphoblastoid cell-lines; the haemagglutination test used human o+ and guine ... | 1988 | 3149932 |
| environmental impact of insect control by microorganisms. | | 1973 | 4198130 |
| [effects of leucocidine and hemolysin from pseudomonas aeruginosa on granulocytes]. | | 1973 | 4200345 |
| beta, gamma unsaturated amino acids as irreversible enzyme inhibitors. | | 1974 | 4210878 |
| letter: a cholinesterase inhibitor produced by aspergillus terreus. | | 1974 | 4212124 |
| [study of the etiology of infectious atrophic rhinitis in swine in bulgaria]. | | 1973 | 4213774 |
| experimental burn wound sepsis--evaluation of enzymatic debridement. | | 1974 | 4214312 |
| [biochemical investigations on the virulentization of some septicemic and blood causative agents. i. oxygen consumption by pseudomonas seruginosa]. | | 1965 | 4958092 |
| [on the relation between the energy of growth activation of microorganisms and the nucleotide composition of their ribosomic rna]. | | 1967 | 4971312 |
| [studies on pseudomonas aeruginosa in surface water. i. sources]. | | 1968 | 4974900 |
| [new tetrahydro-1,3,5-thiadiazine-2-thiones with antimycotic, antibacterial and anthelmintic effect]. | | 1969 | 4984062 |
| mechanism of prophylaxis by silver compounds against infection of burns. | to clarify tthe mechanism by which local application of silver compounds protects burns against infection, an ion-specific electrode was used to measùre the concentration of silver ions in solutions. by this method it was shown that in burn dressings silver ions were reduced to a very low level by precipitation as silver chloride. the antibacterial effect was found to depend on the availability of silver ions from solution in contact with precipitate. between 10(-5) and 10(-6) molar silver nitra ... | 1970 | 4986877 |
| [microbiological problems in experimental animal breeding]. | | 1969 | 5004044 |
| a critical comparison of the hematologic, cardiovascular, and pulmonary response to steroids and nonsteroidal anti-inflammatory drugs in a model of sepsis and adult respiratory distress syndrome. | improved survival of patients receiving high-dose steroid therapy in sepsis and adult respiratory distress syndrome (ards) has been reported, but such therapy and its benefits remain controversial. recently research has been directed toward manipulation of the arachidonic acid cascade. improved survival and hemodynamics with administration of nonsteroidal anti-inflammatory drugs (nsaid) have been reported in animal models of sepsis and ards. the purpose of this study was to compare the effects o ... | 1986 | 3764692 |
| complement depletion in a porcine model of septic acute respiratory disease. | in a porcine model of severe septic acute respiratory failure produced by continuous infusion of live pseudomonas aeruginosa, the role of the complement system was studied by pretreating animals with cobra venom factor (cvf) to deplete c3. three groups of spontaneously breathing animals were monitored with swan-ganz and arterial thermodilution catheters. group i was pretreated with 80 u/kg of cvf iv 16-18 hours before testing. group ii received ps. aeruginosa iv (2 x 10(8)/20 kg/minute). group i ... | 1987 | 3599107 |
| does compliance reflect oxygen delivery in porcine septic respiratory failure treated with positive end-expiratory pressure? | oxygen delivery (do2) in patients with acute respiratory failure has been correlated with total lung and chest wall compliance (ct), the optimal positive end-expiratory pressure (peep) level reportedly corresponds to maximal do2. to test the validity of the relationship, we studied the correlation between ct and do2 in 12 septic pigs with acute respiratory failure induced by continuous infusion of live pseudomonas aeruginosa bacteria. cardiac output, pulmonary and systemic arterial bp, blood gas ... | 1987 | 3539525 |
| aeruginocine typing of pseudomonas aeruginosa originating from dairy cows, their environment and other sources. | | 1983 | 6425213 |
| recombinant alpha 1-antitrypsin pittsburgh attenuates experimental gram-negative septicemia. | alpha 1-antitrypsin-pittsburgh (at-p), a naturally occurring lethal mutation (358met----arg), has been genetically engineered (rat-p). the protein has been shown to be a potent active site-directed inhibitor of thrombin and the contact enzymes factor xiif, factor xia, and kallikrein. because activation of the contact system is known to occur in gram-negative septicemia, the authors have hypothesized that the administration of rat-p might modulate the course of this syndrome. yorkshire piglets an ... | 1988 | 3257651 |
| model for studying bacterial adherence to skin wounds. | the adherence of bacterial pathogens to wounded skin is probably the first step in wound infection. this report describes the development of a bioassay to simulate the adherence of bacteria to wounds. the adherence of bacteria was examined by exposing wounds to known quantities of pathogens, washing the wounds with distilled water, and quantitating the number of adherent bacteria per cm2 of tissue. our studies focused on the effects of naturally occurring mediators of bacterial adherence, such a ... | 1987 | 3116035 |
| disruption of respiratory cilia by proteases including those of pseudomonas aeruginosa. | pseudomonad proteases disrupted the function and structure of demembranated cilia (axonemes) extracted from porcine tracheae. proteolytic degradation by the two pseudomonad proteases elastase and alkaline protease and by trypsin and subtilisin impaired motility of atp-activated axonemes. in addition, electron microscopic observation of negatively stained axonemes indicated that exposure to proteases caused dissociation into individual doublet or singlet microtubules. inhibition of motility and a ... | 1986 | 3095241 |
| pig skin as test substrate for evaluating topical antimicrobial activity. | the feasibility of using pathogen-contaminated pig skin as a model substrate for evaluating skin disinfectants was demonstrated. a test methodology is described that is safe, convenient to use, and adaptable to a variety of hand-washing conditions. the treatment protocol, pathogen contamination conditions, and application technique variables can all be carefully controlled to simulate clinical use conditions. the number of organisms transferred by contact was compared with the total organism cou ... | 1986 | 3093525 |
| pulmonary clearance of blood-borne bacteria. | clearance of blood-borne bacteria has been attributed primarily to a fixed macrophages in the liver and spleen. although many important nonrespiratory functions of the lung have been reported, a major role for this organ in the clearance of circulating bacteria has not been described. to investigate mechanisms of sepsis induced lung dysfunction and pneumonia, we measured lung clearance, tissue accumulation and morphologic effect of blood-borne. pseudomonas aeruginosa in pigs. single pass pulmona ... | 1981 | 6795733 |
| rhamnolipid from pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes. | isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified pseudomonas aeruginosa rhamnolipid. this is a defined ciliary system allowing observation of direct impairment of functional axonemes. axonemal motility and atpase activity were decreased in proportion to rhamnolipid concentrations. atpase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and dynein arms seen in ultra-structural cross sections progre ... | 1986 | 2947698 |
| recent experience with pseudomonas aeruginosa immune globulin for the treatment of experimental pneumonia. | a guinea-pig model of experimental pseudomonas aeruginosa pneumonia was used to determine what factors affect the efficacy of passive immune therapy with a hyperimmune p. aeruginosa globulin (pa-igiv). animals treated 2 h after infection with a single iv infusion of pa-igiv, 500 mg kg-1, demonstrated 33% survival. lower dosages were less effective and there were no survivors among albumin-treated controls. treatment with pa-igiv was effective if given 2 or 8 h after infection but not if delayed ... | 1988 | 2902130 |
| experimental studies on the protective efficacy of a pseudomonas aeruginosa vaccine. | pseudomonas aeruginosa vaccine (pv) containing cell proteins with molecular weight (mr) 20,000-100,000 and up to 0.08% (w/v) admixture of lipopolysaccharide was obtained by water-salt extraction and subsequent ultrafiltration. pv protects mice against experimental p. aeruginosa infection, stimulates production of specific protective antibodies in rabbit and does not provoke obvious toxicity in laboratory animals. | 1989 | 2514518 |
| macromolecular mechanisms of sputum inhibition of tobramycin activity. | tobramycin, an aminoglycoside antibiotic, is used in the treatment of pseudomonas aeruginosa infections in cystic fibrosis patients. tobramycin bioactivity, however, is antagonized by sputum. glycoproteins (mucins) and high-molecular-weight dna make up 2 to 3% (p. l. masson and j. f. heremans, p. 412-475, in m. j. dulfano, ed., sputum: fundamentals and clinical pathology, 1973) and 3 to 10% (w. s. chernick and g. j. barbero, pediatrics 24:739-745, 1959, and r. picot, i. das, and l. reid, thorax ... | 1995 | 7535039 |
| ranitidine compared to cimetidine in multiagent pharmacological treatment of porcine pseudomonas ards. | the effects of two pharmacologically distinct histamine h2 receptor antagonists were studied in combination with ibuprofen (i) and diphenhydramine (d) in a porcine model of septic ards. cimetidine (c) is reported as having direct oxygen radical scavenging abilities and is an inhibitor of cytochrome p-450, whereas ranitidine (r) acts solely by h2 receptor blockade. four groups were studied: group ps (n = 8) received a continuous infusion of live pseudomonas aeruginosa 5 x 10(8) cfu/ml at 0.3 ml/2 ... | 1990 | 2311202 |
| increased survival time after delayed histamine and prostaglandin blockade in a porcine model of severe sepsis-induced lung injury. | a combination of cimetidine, diphenhydramine, and ibuprofen has been shown to be an effective treatment in a porcine model of septic acute lung injury. the present study was designed to evaluate this therapy in a delayed treatment survival model. three groups of animals were studied: a control group (c, n = 6) received a sham infusion of 0.9% saline; a septic group (ps, n = 5) received a continuous infusion of live pseudomonas aeruginosa organisms; and a treatment group (cid, n = 6) received p. ... | 1990 | 2302957 |
| occlusive dressings. does dressing type influence the growth of common bacterial pathogens? | we studied the effect of different occlusive dressings and of air exposure on the growth of four pathogenic bacteria in wounds. partial-thickness wounds on domestic pigs were inoculated with staphylococcus aureus, clostridium perfringens, bacteroides fragilis, or pseudomonas aeruginosa. each wound was covered with three dressings (duoderm, opsite, or vigilon), or left exposed to air. groups of wounds were sampled at 24, 48, and 72 hours. staphylococcus aureus reached high levels beneath all of t ... | 1990 | 2119166 |
| further characterization of pasteurella haemolytica-like bacteria isolated from swine enteritis. | dna-dna hybridization studies were conducted on six pasteurella haemolytica-like (phl) organisms recovered from cases of swine enteritis. chromosomal-enriched fractions of phl organisms served as the source of dna for southern blots or as whole-chromosomal dna probes. under stringent hybridization conditions, chromosomal dna probes of a prototype phl (strain 6213a) organism distinguished other phl organisms from pasteurella haemolytica types a1 and t3, pasteurella multiocida types a:1 and a:3, e ... | 1993 | 8460470 |
| synthesis and characterization of escherichia coli o18 o-polysaccharide conjugate vaccines. | nontoxic, serologically reactive o polysaccharide was derived from escherichia coli o18 lipopolysaccharide by acid hydrolysis, extraction with organic solvents, and gel filtration chromatography. oxidized o polysaccharide was covalently coupled to either pseudomonas aeruginosa toxin a or cholera toxin by using adipic acid dihydrazide as a spacer molecule in the presence of carbodiimide. the resulting conjugates were composed of approximately equal amounts of o polysaccharide and protein and were ... | 1990 | 2105272 |
| pulmonary compliance: early assessment of evolving lung injury after onset of sepsis. | we compared the sensitivity of dynamic (cdyn) and static lung compliance (cl) with indicators of permeability injury in a model of septic porcine adult respiratory distress syndrome. two groups of anesthetized ventilated swine (15-25 kg) were studied. septic animals (ps, n = 13) received pseudomonas aeruginosa intravenously for 1 h, which resulted in severe adult respiratory distress syndrome. controls (c, n = 13) received 0.9% nacl. cdyn, cl, bronchoalveolar lavage for protein estimation, and t ... | 1990 | 2077027 |
| hemodynamic effects of lobar pulmonary artery occlusion in a porcine sepsis model. | we induced severe pulmonary hypertension and acute lung injury in 6 pigs by pseudomonas aeruginosa infusion. we studied the effect of pulmonary artery catheter inflation of a pulmonary artery catheter balloon in the left lower lobar pulmonary artery was accompanied by a significant (p less than 0.05, paired t test) increase in pulmonary artery pressure, a decrease in left atrial pressure, a decrease in cardiac output, and a decrease in mean arterial pressure. no significant changes occurred when ... | 1991 | 1998424 |
| drug-susceptibility and isolation of a plasmid in haemophilus (actinobacillus) pleuropneumoniae. | we isolated 56 haemophilus (actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. two drug-resistant strains were obtained. one, named kh-265, was resistant to streptomycin (sm) and sulfonamide (sa), and the other, named kh-195, was resistant to tetracycline (tc). the minimum inhibitory concentrations (mics) of drugs for resistant strains were 100 micrograms/mliters for sm, 3200 micrograms/mliters for sa, and 12.5 micro ... | 1990 | 1968988 |
| nonaldehyde sterilization of biologic tissue for use in implantable medical devices. | biologic tissue stabilized by dye-mediated photooxidation has found application in implantable devices. the desire to avoid aldehydes in the processing of photooxidized tissues led to the development of a nonaldehyde, iodine based sterilant. the interaction of tissue with iodine was indicated by a change in tissue shrinkage temperature, dependent upon solution and incubation parameters. the amino acid tyrosine also was altered, presumably because of aromatic ring iodination. transmission electro ... | 1997 | 9116349 |
| chimeric cytotoxin il2-pe40 inhibits relapsing experimental allergic encephalomyelitis. | il2-pe40 is a chimeric protein composed of human interleukin-2 (il2) genetically fused to a modified form of pseudomonas exotoxin lacking the cell recognition domain. il2-pe40 is cytotoxic for il2 receptor-bearing lymphocytes in culture and can inhibit activation of t cells in vivo. il2-pe40 can significantly diminish antigen-stimulated proliferation of lymphocytes sensitized to myelin basic protein. intraperitoneal administration of il2-pe40 not only markedly inhibits the clinical manifestation ... | 1991 | 1709644 |
| cd18 adhesion receptors, tumor necrosis factor, and neutropenia during septic lung injury. | sequestration of neutrophils (pmns) in the pulmonary microvasculature and associated neutropenia are characteristic features of experimental models of septic lung injury. the etiology of altered pmn kinetics during septic lung injury is uncertain, but may be partially due to increased adhesiveness of activated pmns to pulmonary endothelium. this study examines the relationship between the expression of pmn cd18 adhesion receptors, the evolving neutropenia, and plasma tumor necrosis factor (tnf) ... | 1991 | 1673483 |
| delayed cyclo-oxygenase blockade reduces the neutrophil respiratory burst and plasma tumor necrosis factor levels in sepsis-induced acute lung injury. | ibuprofen pretreatment attenuates the enhanced neutrophil (pmn) respiratory burst and reduces increased plasma tumor necrosis factor (tnf) activity in porcine sepsis-induced acute lung injury (ali). these septic responses have been linked to increased alveolar-capillary membrane (acm) permeability. this study was designed to establish whether delayed ibuprofen treatment would have the same effect and to examine the relationship between pmn oxidant generation and tnf. three groups of anesthetized ... | 1991 | 1647464 |
| the effect of spermatozoa and seminal plasma on leukocyte migration into the uterus of gilts. | yorkshire x landrace gilts were used to determine the effect of spermatozoa and seminal plasma on postbreeding uterine leukocyte influx. estrus detection was performed with a boar at 12-h intervals following synchronization with 400 iu ecg and 200 iu of hcg. all gilts were ai once, 24 h after the detection of estrus following random assignment to a 2x2x3 factorial arrangement of treatments (sperm or sperm-free ai doses), ai dose medium (seminal plasma or pbs), and lavage time following ai. gilts ... | 1999 | 10462000 |
| identification of an anti-mycobacterial domain in nk-lysin and granulysin. | nk-lysin and granulysin are homologous cationic anti-bacterial peptides produced by pig and human cytolytic lymphocytes, respectively. the solution structure of nk-lysin comprises five amphipathic alpha-helices. to investigate the properties of a helix-loop-helix region postulated to be a membrane-docking part of nk-lysin, we synthesized 22- and 29-residue peptides reproducing this region for both nk-lysin and granulysin. cd spectroscopy of the synthetic peptides in a liposomal solution showed s ... | 1999 | 10585872 |
| interaction of the pseudomonas aeruginosa secretory products pyocyanin and pyochelin generates hydroxyl radical and causes synergistic damage to endothelial cells. implications for pseudomonas-associated tissue injury. | pyocyanin, a secretory product of pseudomonas aeruginosa, has the capacity to undergo redox cycling under aerobic conditions with resulting generation of superoxide and hydrogen peroxide. by using spin trapping techniques in conjunction with electron paramagnetic resonance spectrometry (epr), superoxide was detected during the aerobic reduction of pyocyanin by nadh or porcine endothelial cells. no evidence of hydroxyl radical formation was detected. chromium oxalate eliminated the epr spectrum o ... | 1992 | 1469082 |
| neutrophil short-lived oxidant production: enhancement following onset of sepsis-induced lung injury. | generation of superoxide anion (o2-) by activated neutrophils (pmn) is implicated in the pathogenesis of endothelial cell injury in sepsis. to quantitate this phenomenon we studied the kinetics of o2- production by pmn following in vivo and in vitro exposure to pseudomonas aeruginosa. pmn were isolated from young swine before and after a 1-hr infusion with 5 x 10(8) organisms/ml at 0.3 ml/20 kg/min. baseline pmn were studied in an in vitro system where 1 x 10(6) porcine pmn were incubated with l ... | 1992 | 1320466 |
| a mycobacteriology proficiency testing program using simulated sputum specimens. | the new york state department of health proficiency testing program in mycobacteriology determines the ability of a laboratory to isolate the acid-fast bacillus present in a simulated specimen and to identify the strain. until 1964 the specimens were autoclaved normal sputa seeded with mycobacteria. when mandatory testing was introduced by law in 1964, more specimens were needed. these have been prepared in simulated sputum bases, first skimmed milk, then granulated hog gastric mucin. the bases ... | 1976 | 819397 |
| [problems of the immunoprophylaxis of experimental pseudomonas pyocyanea wound sepsis]. | an experimental study showed that a multicomponent pseudomonas aeruginosa preparation, developed by the authors and named "pyoimmunogen", had a pronounced protective effect against the culture of p. aeruginosa, most frequently isolated in surgical clinics. the immunization of rats carried out in accordance with a specially devised scheme allowed to decrease mortality rate in experimental pyocyanic wound sepsis to 6.9%, whereas in the control animals mortality rate reached 91.5%. | 1979 | 110018 |
| mortality among fowls due to pseudomonas aeruginosa. | | 1969 | 5389545 |
| assessment of structural features of the pseudomonas siderophore pyochelin required for its ability to promote oxidant-mediated endothelial cell injury. | we previously showed that iron chelated to the pseudomonas aeruginosa siderophore pyochelin enhances oxidant-mediated injury to pulmonary artery endothelial cells by catalyzing hydroxyl radical (ho(*)) formation. therefore, we examined pyochelin structural/chemical features that may be important in this process. five pyochelin analogues were examined for (i) capacity to accentuate oxidant-mediated endothelial cell injury, (ii) ho(*) catalytic ability, (iii) iron transfer to endothelial cells, an ... | 2001 | 11556810 |
| synthesis and antibacterial evaluation of novel 2-[n-imidoylpyrrolidinyl] carbapenems. | the synthesis, antibacterial activity and dhp-susceptibility of a series of novel carbapenems, directly linked with heterocyclic moiety are described. especially, the compounds linked pyrrolidine-carbapenem exhibited to have a good antibacterial activity against staphylococcus aureus (mrsa) as well as pseudomonas aeruginosa to maintain a good stability towards dhp-i. | 2002 | 11814802 |
| prostacyclin in experimental septic acute respiratory failure. | manipulation of prostaglandins (pg) in animal models of sepsis and acute respiratory failure (arf) is promising. prostacyclin (pgi2), a short-acting vasodilator, was evaluated in a porcine model of arf produced by continuous infusion of live pseudomonas aeruginosa (ps.). cardiopulmonary parameters were monitored in three groups of spontaneously breathing animals that received 0.1 micrograms pgi2/kg/min begun 20 min after baseline (group i); 2 x 10(8) ps./20 kg/min (group ii); identical ps. infus ... | 1983 | 6339818 |
| biofilm bacteria: formation and comparative susceptibility to antibiotics. | the calgary biofilm device (cbd) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. the minimum inhibitory concentration (mic) and minimum biofilm eradication concentration (mbec) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin g, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative b ... | 2002 | 11989739 |
| [microbiological analysis of experimental burn wounds in swine during treatment with silver sulfadiazine]. | | 1983 | 6423917 |
| pseudomonas aeruginosa infection-evidences of amphixenosis. | in the present investigation many of the isolates of pseudomonas aeruginosa from human beings, poultry and pigs fell in the same aeruginocine type. aeruginocine a1 of ps. aeruginosa was isolated from pigs and also from one of the pig attendants on the farm. majority of the strains of ps. aeruginosa of the same aeruginocine type also exhibited similar drug sensitivity patterns. the organism appeared to be polyphagous in nature and infection with this agent may be considered as amphixenosis. | 1984 | 6438011 |
| defense mechanisms of urinary bladder: studies on antimicrobial polypeptides from bladder mucosa. | the acid-soluble extract of the bladder mucosal surface was obtained by washing out the bladder with dilute acetic acid in the presence of protease inhibitors. the wash-out materials from rats, rabbits, pigs, and humans manifested strong bactericidal activity against e. coli in vitro. the ultrafiltrate of the human material, which contained two major peptides with apparent molecular masses of 6.7 kd and 8.5 kd, respectively, showed potent bactericidal activity against e. coli, pseudomonas aerugi ... | 1999 | 12899379 |
| the effects of pulmonary atelectasis and reexpansion on lung cellular immune defenses. | pulmonary atelectasis predisposes the lung to infection. this condition may be partly due to impaired cellular immune response of the collapsed lung segment. we postulated that atelectasis may affect alveolar macrophage (am) antibacterial function. to test this hypothesis, atelectasis was induced in the right upper lobes of piglet lungs. alveolar macrophages harvested by bronchoalveolar lavage of collapsed segments for up to 24 hours showed progressive depression of their phagocytic activity aga ... | 1984 | 6696620 |
| studies on septicemia in extensive burns. | | 1964 | 14239695 |
| in vivo and in vitro antimicrobial activity of silver sulfadiazine and cerium nitrate. | the antimicrobial activity of cerium nitrate and silver sulfadiazine was assessed in vitro and in vivo using pseudomonas aeruginosa. in vitro, the activity of silver sulfadiazine was significantly greater than that of cerium nitrate. synergism between silver sulfadiazine and cerium nitrate was observed in water or saline solution suspensions, but not in broth. in vivo, cerium nitrate offered no therapeutic benefit in reducing wound infection in contaminated wounds. treatment of similar wounds wi ... | 1980 | 6773164 |
| antiphagocytic effect of pseudomonas aeruginosa exopolysaccharide. | | 1980 | 6778898 |
| [is the resorbable, porous tricalcium phosphate ceramic suitable for the obliteration of not positively aseptic ear radical cavities? an animal experimental study of the hypotympanum of the swine]. | | 1982 | 6811812 |
| [combined staphylococcus-proteus-pseudomonas vaccine. i. effectiveness of the vaccine in experimental pseudomonas aeruginosa infections]. | | 1981 | 7331610 |
| sepsis-induced acute lung injury is attenuated by selectin blockade following the onset of sepsis. | to determine the effect of infusion with a dual-binding antibody to e- and l-selectin, el-246, in a postonset model of sepsis. | 1995 | 7487463 |
| characteristics of pseudomonas aeruginosa strains isolated from urinary tract infections. | thirty-three uropathogenic strains of pseudomonas aeruginosa were investigated for hemolytic activity in both bacterial broth culture filtrates and isolate lyzates, resistance to bactericidal activity of fresh human serum, resistance to six antibiotics and plasmid dna profile. twenty-four of the 33 (73%) bacterial filtrates showed lysis of rabbit erythrocytes, as did the three after guinea-pig erythrocyte treatment. twelve of 33 isolate lysates showed in parallel lysis of both types of erythrocy ... | 1994 | 7729771 |
| release of endothelin in relation to tumor necrosis factor-alpha in porcine pseudomonas aeruginosa-induced septic shock. | septic shock is characterized by surges of tumor necrosis factor-alpha (tnf-alpha) along with myocardial dysfunction and systemic hypotension. tnf-alpha promotes the release of immunoreactive endothelin (et). because tnf-alpha is elevated in septic shock, we hypothesized that elevated levels of endothelin can contribute to cardiac dysfunction and hypotension. we infused live pseudomonas aeruginosa into anesthetized, hemodynamically monitored young swine and measured et and tnf-alpha. septic swin ... | 1994 | 7743336 |
| pseudomonas siderophore pyochelin enhances neutrophil-mediated endothelial cell injury. | pyochelin, a siderophore secreted by pseudomonas aeruginosa, binds iron in a form which can catalyze the formation of hydroxyl radical (.oh) from neutrophil-derived superoxide (o2-.) and hydrogen peroxide (h2o2). ferripyochelin induced a concentration-dependent increase in endothelial cell injury (51cr release) resulting from exposure to h2o2, a xanthine/xanthine oxidase o2-./h2o2 generating system, or stimulated neutrophils. this process was dependent on the presence of iron. formation of .oh w ... | 1994 | 8141315 |
| a bacterial protease perturbs the paracellular barrier function of transporting epithelial monolayers in culture. | tight junctions between cells and adhesion to the substratum maintain the barrier function of epithelia throughout the body. damage to the epithelial barrier by microbial products allows penetration of bacteria and promotion of infection. we studied the effects of pseudomonas elastase (pe) on the barrier function of epithelia by using madin-darby canine kidney (mdck) epithelial cells; these cells form tight junctions (zonula occludens [zo]) in vitro. pe decreased electrical resistance across the ... | 1993 | 8500907 |
| efficacy of meropenem in experimental meningitis. | meropenem and comparator antibiotics, including ceftriaxone, ceftazidime, benzyl penicillin and a combination of ampicillin plus gentamicin, were evaluated in a model of bacterial meningitis in the guinea-pig. the model is an acute infection in which challenge with each organism, if untreated, causes an increase in numbers of white blood cells, elevation of protein concentrations and 6-8 log10 cfu/ml of bacteria in the csf. infections caused by haemophilus influenzae, neisseria meningitidis, thr ... | 1995 | 8543501 |
| antimicrobial disinfection effect of a laundering procedure for hospital textiles against various indicator bacteria and fungi using different substrates for simulating human excrements. | recent studies confirm the increase of nosocomial infections and microbial resistance. one of the possible causes is infected textiles due to inappropriate laundering procedures. most slovenian laundries use thermal laundering procedures with high energy and water consumption to disinfect hospital textiles. in addition to this fact, there is an increasing number of hospital textiles composed of cotton/polyester blends that cannot endure high temperatures of thermal disinfection. on the other han ... | 2007 | 17046191 |
| pulmonary artery endothelial cell function in swine pseudomonas sepsis. | a substantial increase in pulmonary vascular resistance is associated with sepsis and its sequelae (sepsis syndrome and septic shock). it is postulated that increased resistance may result from sepsis-induced endothelial cell injury or altered vasoreactivity secondary to pulmonary hypertension. we, therefore, tested the hypothesis that sepsis causes endothelial cell injury and that increased pulmonary pressure alters vascular reactivity. young swine (15-25 kg) were anesthetized and ventilated. s ... | 1996 | 8592413 |
| non-toxic pseudomonas aeruginosa exotoxin a expressing the fmdv vp1 g-h loop for mucosal vaccination of swine against foot and mouth disease virus. | synthetic peptides derived from the g-h loop of the foot and mouth disease virus (fmdv) capsid protein vp1 are relatively poor at recapitulating the native conformation present in the virus, and thus are often poor immunogens. we hypothesized that a candidate mucosal vaccine against fmdv could be developed using the non-toxic pseudomonas aeruginosa exotoxin a (ntpe) to deliver the g-h loop in its native conformation. an added benefit of this approach is the potential for ntpe to serve as an effe ... | 2007 | 17276557 |
| in vitro and in vivo antibacterial activities of er-35786, a new antipseudomonal carbapenem. | er-35786 is a new parenteral 1 beta-methyl carbapenem with a broad antibacterial spectrum and a potent antipseudomonal activity. it showed high in vitro activity, comparable to those of meropenem and a new carbapenem, bo-2727, against methicillin-susceptible staphylococcus aureus and streptococci, with mics at which 90% of strains tested are inhibited (mic90s) of < or = 0.39 microgram/ml. against methicillin-resistant s. aureus, er-35786 was the most active among the compounds tested, yet its mi ... | 1997 | 9021183 |
| biochemical characterization of a flavin adenine dinucleotide-dependent monooxygenase, ornithine hydroxylase from pseudomonas aeruginosa, suggests a novel reaction mechanism. | pyoverdin is the hydroxamate siderophore produced by the opportunistic pathogen pseudomonas aeruginosa under the iron-limiting conditions of the human host. this siderophore includes derivatives of ornithine in the peptide backbone that serve as iron chelators. pvda is the ornithine hydroxylase, which performs the first enzymatic step in preparation of these derivatives. pvda requires both flavin adenine dinucleotide (fad) and nicotinamide adenine dinucleotide phosphate (nadph) for activity; it ... | 2007 | 17900176 |
| synthesis and antibacterial action of cecropin and proline-arginine-rich peptides from pig intestine. | two antimicrobial peptides, cecropin p1 (cp1), with a c-terminal carboxyl group, and pr-39, with an amidated, c-terminus, are found in the small intestine of the pig. each is active against both gram-positive and gram-negative bacteria. we have synthesized these peptides and several analogs, including the d-enantiomers and the retro sequences, each with a free or acetylated amino terminus. the cp1 amide was also prepared. the retro cp1 peptides were much less active than the parent cp1 peptide, ... | 1997 | 9128101 |
| pretreatment with inhaled nitric oxide inhibits neutrophil migration and oxidative activity resulting in attenuated sepsis-induced acute lung injury. | to determine if, and by what mechanisms, inhaled nitric oxide attenuates acute lung injury in a porcine model of adult respiratory distress syndrome induced by gram-negative sepsis. | 1997 | 9142021 |
| identification of a 25-aminoacid sequence from the major african swine fever virus structural protein vp72 recognised by porcine cytotoxic t lymphocytes using a lipoprotein based expression system. | identification of african swine fever virus (asfv) proteins recognised by cytotoxic t lymphocytes (ctl) from swine surviving asfv/nh/p68 infection was assessed using expression vectors based on the pseudomonas aeruginosa outer membrane lipoprotein i gene (opri). viral antigens expressed as fusion lipoproteins were shown to be taken efficiently by porcine blood-derived macrophages incubated with outer membrane protein preparations from transformed e. coli. to assess recognition by ctl the fusion ... | 1998 | 9820580 |
| isolation of pseudomonas aeruginosa from ushubetsu river water in hokkaido, japan. | to provide information on the ecology of pseudomonas aeruginosa in nature, bacteriological surveillance was performed in the defined area in hokkaido, japan. p. aeruginosa was isolated from water samples of ushubetsu river in the down stream from the urban area of asahikawa. p. aeruginosa was isolated from fecal samples of pigs but not from samples of soil of a tomato field, sand of sandboxes in vest-pocket parks, fresh vegetables, or feces of wild deer. the present results indicate that p. aeru ... | 2000 | 10927883 |
| tryptophan end-tagging of antimicrobial peptides for increased potency against pseudomonas aeruginosa. | due to increasing antibiotics resistance, antimicrobial peptides (amps) are receiving increased attention. pseudomonas aeruginosa is a major pathogen in this context, involved, e.g., in keratitis and wound infections. novel bactericidal agents against this pathogen are therefore needed. | 2009 | 19345721 |
| chronic porcine two-hit model with hemorrhagic shock and pseudomonas aeruginosa sepsis. | sepsis is still a major cause of death despite well-developed therapeutical strategies such as antibiotics and supportive medication. the aim of this study was to characterize the long-term effects of a two-hit porcine sepsis model with a hemorrhagic shock as 'first hit' followed by a pseudomonas aeruginosa infusion as 'second hit'. | 2002 | 11867903 |
| early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. | a porcine model of wound healing was employed to examine the impact of nanocrystalline silver-coated dressings on specific wound healing events. full-thickness wounds were created on the backs of pigs, contaminated with an experimental inoculum containing pseudomonas aeruginosa, fusobacterium sp., and coagulase-negative staphylococci, and covered with dressing products either containing silver or not. nanocrystalline silver-coated dressings promoted rapid wound healing, particularly during the f ... | 2002 | 12100375 |
| in vivo performance of melimine as an antimicrobial coating for contact lenses in models of clare and clpu. | one strategy to minimize bacteria-associated adverse responses such as microbial keratitis, contact lens-induced acute red eye (clare), and contact lens induced peripheral ulcers (clpus) that occur with contact lens wear is the development of an antimicrobial or antiadhesive contact lens. cationic peptides represent a novel approach for the development of antimicrobial lenses. | 2010 | 19710414 |
| cp0569, a new broad-spectrum injectable carbapenem. part 1: synthesis and structure-activity relationships. | a series of 1beta-methylcarbapenems bearing an (imidazo[5,1-b]thiazolium-6-yl)methyl moiety, a 5,5-fused heterobicycle, at the c-2 position was synthesized and evaluated for in vitro antibacterial activities. cp0569 (1r) and its analogues showed potent antibacterial activities against gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa), and gram-negative bacteria, including pseudomonas aeruginosa. moreover, cp0569 (1r) exhibited stronger antibacterial activity ag ... | 2003 | 12878141 |
| natural antimicrobial agent (reuterin) produced by lactobacillus reuteri for sanitization of biological tissues inoculated with pseudomonas aeruginosa. | the study was done to evaluate the efficacy of using reuterin produced by lactobacillus reuteri to sanitize biological tissues. the microorganism tested in the study was pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. the inhibitory effect of reuterin on p. aeruginosa for an inoculated tissue was investigated at different conditions of concentration, temperature, and ph. additionally, the cellular compatibility of the reuterin-sanitized tissue was evaluated. ... | 2003 | 12966580 |
| sepsis-induced failure of hepatic energy metabolism. | recent evidence suggests that sepsis may induce an uncoupling of oxidative phosphorylation. the purpose of this study was to quantify temporal changes in hepatic oxygen consumption and cellular energy state with increasing severity of sepsis and thus assess the interrelationship of these parameters as either primary defect or compensatory response. | 2003 | 14572785 |
| hearing loss with stapedotomy in otitis media. | stapes fixation is often found in patients with either active or inactive otitis media (om). stapedotomy is generally not performed in ears with om because of the potential risk of sensorineural hearing loss (snhl). recent studies have demonstrated that the inner ear may be fenestrated in the presence of om without significantly increased risk of snhl. the goal of this study was to determine if stapedotomy in the presence of om significantly increases the incidence of postoperative snhl. | 2003 | 14663433 |
| cecropin p1 and novel nematode cecropins: a bacteria-inducible antimicrobial peptide family in the nematode ascaris suum. | cecropin p1 was first identified as a mammalian antimicrobial peptide isolated from the pig intestine. much research aimed at characterizing this peptide has been reported. recently, the workers who discovered the peptide corrected their original conclusion, and confirmed that this peptide originates in fact from the pig intestinal parasitic nematode, ascaris suum. in the present study, we carried out a semi-exhaustive search for bacteria-inducible transcripts in a. suum by the cdna subtraction ... | 2005 | 15850460 |
| antimicrobial-bonded graft patency in the setting of a polymicrobial infection in swine (sus scrofa). | polytetrafluoroethylene (ptfe) and dacron are commonly used as arterial conduits in vascular trauma or infection when vein interposition graft may not be available. this study used a previously validated large animal model of polymicrobial infection to assess the patency and infectious resistance of a novel, antibiotic-impregnated graft material compared with ptfe and dacron. | 2016 | 27988159 |
| effects of tempol, a free radical scavenger, on long-term hyperdynamic porcine bacteremia. | pretreatment with tempol, a membrane-permeable radical scavenger, has been shown to be protective in rodent models of endotoxic and gram-positive shock. however, neither the pretreatment design nor hypodynamic endotoxic shock in rodents mimics the clinical scenario. therefore, we investigated the effects of tempol in a posttreatment model of long-term, volume-resuscitated, hyperdynamic porcine bacteremia. | 2005 | 15891336 |
| analyses of pseudomonas aeruginosa lectin binding to alpha-galactosylated glycans. | the specificity and binding capacity of the galactophilic lectin from the gram negative bacterium pseudomonas aeruginosa (pa-il) was determined by solid phase measurements using galactosylated neoglycoproteins immobilized on microtiter plates. the bacterial lectin reacted with both short chain (monosaccharide) and long chain (pentasaccharide) glycoconjugates. among the galalpha1-xgal disaccharides, the highest affinity was observed towards the galalpha1-3gal structure. raising the incubation tem ... | 2005 | 15968505 |
| miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray. | an enzyme-based glucose biosensor modified to release nitric oxide (no) via a xerogel microarray is reported. the biosensor design is as follows: (1) glucose oxidase (gox) is immobilized in a methyltrimethoxysilane (mtmos) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide no-release capability. this configurat ... | 2005 | 16242614 |
| perfusion pressure manipulation in porcine sepsis: effects on intestinal hemodynamics. | limited information is available about selection of the threshold for arterial blood pressure in critically ill patients, particularly in sepsis when normal organ blood flow autoregulation may be altered. the present experimental study investigated whether increasing perfusion pressure using norepinephrine in normotensive hyperdynamic porcine bacteremia affects intestinal macro- and microcirculation. nine pigs received continuous i.v. administration of pseudomonas aeruginosa (psae) to develop hy ... | 2006 | 16343046 |
| effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media. | to determine the role of nitric oxide (no) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (ome). | 2006 | 16399192 |
| evaluation of a bi-layer wound dressing for burn care. ii. in vitro and in vivo bactericidal properties. | we have recently designed a medicated bi-layer wound dressing to address the key requirements for treating external, contaminated war wounds. this study assessed the in vitro and in vivo bactericidal efficacies of the drdc hydrogel/polyurethane wound dressing. chloramphenicol- and chlorhexidine-loaded drdc dressings produced significantly larger zones of inhibition against pseudomonas aeruginosa than the other medicated dressings for 4 d. chlorhexidine-loaded allevyn and hydrasorb remained bacte ... | 2006 | 16455202 |
| lung deposition of continuous and intermittent intravenous ceftazidime in experimental pseudomonas aeruginosa bronchopneumonia. | lung tissue deposition of intravenous ceftazidime administered either continuously or intermittently was compared in ventilated piglets with experimental bronchopneumonia. | 2006 | 16858611 |
| coagulation and endothelial dysfunction during longterm hyperdynamic porcine bacteremia--effects of selective inducible nitric oxide synthase inhibition. | coagulation abnormalities have been implicated in the pathogenesis of sepsis and organ dysfunction. nitric oxide (no) is regarded as a critical mediator of many vascular pathologies, including sepsis. however, limited evidence is available to document a relationship between no generated by inducible no synthase (inos) and hemostatic abnormalities in sepsis. therefore, we evaluated the effects of selective inos inhibition on markers of endothelial and coagulation homeostasis in a clinically relev ... | 2007 | 17264961 |
| experimental pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response. | an abnormal inflammatory response (ir) in pneumonia is associated with poor outcomes and high mortality. animal models could help to better understand the relationship between the pulmonary infection and the associated ir. the aims of the present study were to validate an experimental model of pneumonia induced by the inoculation of pseudomonas aeruginosa in ventilated piglets and to study the associated ir over a long period of time (96 h). five lagerwhite-landrace piglets were ventilated for 4 ... | 2007 | 17804447 |