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rheumatoid arthritis: review of searches for an infectious cause. part ii. 197992461
[immunogenic properties of cl. perfringens anatoxins obtained from purified toxins]. 1975176848
[disease of newborn piglets caused by c1. perfringens]. 1976186937
changes in lipid metabolism and cell morphology following attack by phospholipase c (clostridium perfringens) on red cells or lymphocytes.when intact human erythrocytes were treated with phospholipase c (clostridium perfringens), up to 30% of the membrane phospholipids were broken down without significant cell lysis. only phosphatidylcholine and sphingomyelin were attacked. ceramide (derived from sphingomyelin) accumulated, but 1,2-diacylglycerol (derived from phosphatidylcholine) was largely converted into phosphatidate. up to 12% of the cell phospholipid could be converted into phosphatidate in this way. pig erythrocytes and lym ...1975172156
enterococci in lambs. 19852862641
in vitro tests for the measurement of clostridial toxins, toxoids and antisera. ii. titration of clostridium perfringens toxins and antitoxins in cell culture.the usefulness of cytopathic indicators for the titration of cl perfringens beta and epsilon toxins has been investigated. neutralization experiments with monoclonal antibodies have shown that the entities responsible for the lethal and dermonecrotic effects of cl perfringens beta toxin preparations are identical. however, the cytopathic effects of the same preparations are caused by other entities. therefore, titrations based upon lethal and dermonecrotic indicators of beta toxin are equally va ...19902285500
soluble and cell-associated haemagglutinins of helicobacter (campylobacter) pylori.some plate-grown strains of helicobacter (campylobacter) pylori that were harvested into phosphate-buffered saline and left for 1 h released soluble haemagglutinins. these caused high-titre agglutination of human and guinea-pig erythrocytes, whereas chicken, sheep and bovine erythrocytes were agglutinated at various titres. six of 10 strains which had been subcultured repeatedly did not possess soluble haemagglutinins. slide agglutination of bacterial suspensions demarcated the strains into two ...19902258915
purification and some effects of clostridium welchii type c beta toxin. 1979233179
[enterotoxin detection in thermostable clostridium perfringens type a strains isolated from a food poisoning outbreak (author's transl)]. 19744155890
the occurrence of clostridium welchii type c in papua new guinea. 1979233178
clostridium welchii type c antitoxin in the treatment of "pig bel" (enteritis necroticans): a controlled trial in papua new guinea.enteritis necroticans remains a serious problem in the highlands of papua new guinea. earlier claims that clostridium welchii type c antitoxin is effective in lowering mortality rates were subjected to a prospective controlled trial in 1972-73. use of the antitoxin was found to be of no benefit in treatment of this disease.1979233177
the pathogenesis of pig-bel in papua new guinea. 1979233175
[studies of necrotizing enteritis of suckling piglets (clostridium perfringens type c enterotoxemia) in industrialized sow breeding units. 4. epizootiology].necrotising enteritis had been the cause of death of 4.9 per cent in 5,177 nursed piglets, which was established by pathological examination. the number of piglets, in that context, which had come from industrialised sow breeding units was equivalent to 92 per cent. the nursed piglet held the third position, next to smaller ruminants (19.4 per cent) and fowl (6.0 per cent), with regard to the occurrence of clostridium perfringens enterotoxemia or necrotising enteritis in 112,218 animals which we ...1979232840
enterotoxin production by lecithinase-positive and lecithinase-negative clostridium perfringens isolated from food poisoning outbreaks and other sources. 1979232099
[experimental studies on animals on the use of pyrrolidino-methyl-tetracycline and penicillin g on the prophylaxis and therapy of gas gangrene]. 19664299480
[virulence of clostridium perfringens d-the pathogene of sheep enterotexemia]. 19664303711
intestinal clostridium perfringens in arthritis and parakeratosis induced by dietary factors. experimental studies in pigs. 19674304126
[epizootiology of clostridium infections]. 19674304375
[conditions for c1. perfringens type a enterotoxin formation in vitro]. 1979229663
contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut. 1979222722
system for evaluating clostridial inhibition in cured meat products.a method for evaluating inhibition of clostridium botulinum, c. sporogenes, and c. perfringens in cured meat products was developed. this system can easily be used in the microbiology laboratory using aluminum ointment tubes as the product container. swells caused by gas production by the organism are easily observed by using the aluminum tubes. results obtained confirmed earlier work on the inhibitory effect of sodium nitrite and sorbic acid against the clostridia in cured meat products.1978211934
isolation and characterization of multiply antibiotic-resistant clostridum perfringens strains from porcine feces.multiply antibiotic-resistant strains of clostridium perfringens were isolated from porcine feces. strains that were resistant to tetracycline, erythromycin, clindamycin, and lincomycin were isolated, but no penicillin- or chloramphenicol-resistant strains were obtained. typical minimal inhibitory concentrations for resistant strains were 16 to 64 mug of tetracycline per ml, 64 to >128 mug of erythromycin per ml, >/=128 mug of lincomycin per ml, and 16 to 128 mug of clindamycin per ml. resistanc ...1978208463
[study of the variability of cl. perfringens type d]. 1977199982
vaccination of pregnant sows with clostridium perfringens type c toxoid. 1977194385
vascular permeability increase by alpha-toxin (phospholipase c) of clostridium perfringens. 1977190737
[presence and pathogenic significance of clostridium perfringens type a in swine]. 1976187166
[clostridium intoxications in swine]. 19714325233
model experiment for inducing gastroenteritis in albino rats. 19704325334
[occurrence of clostridium perfringens and its toxins in the intestinal tract of swine and its significance as an etiologic agent]. 19704326575
the presence of enterotoxigenic clostridium perfringens strains in faeces of various animals.the presence of clostridium perfringens in faeces of horses, cattle, poultry and pigs was determined. c. perfringens was detected in 24%, 36%, 80% and 2% of the faecal samples, respectively. faecal samples containing enterotoxigenic strains as assessed by colony hybridization amounted to 14%, 22%, 10% and 0% respectively.19911777387
[serum inhibitors of clostridium perfringens alpha toxin]. 19862938745
[sensitizing properties of purified concentrated cl. perfringens toxoids]. 19714330843
comments on clostridia and enteric diseases in swine. 19724337843
occlusive dressings. does dressing type influence the growth of common bacterial pathogens?we studied the effect of different occlusive dressings and of air exposure on the growth of four pathogenic bacteria in wounds. partial-thickness wounds on domestic pigs were inoculated with staphylococcus aureus, clostridium perfringens, bacteroides fragilis, or pseudomonas aeruginosa. each wound was covered with three dressings (duoderm, opsite, or vigilon), or left exposed to air. groups of wounds were sampled at 24, 48, and 72 hours. staphylococcus aureus reached high levels beneath all of t ...19902119166
inhibition of phospholipases by met-leu-phe-ile-leu-ile-lys-arg-ser-arg-his-phe, c terminus of middle-sized tumor antigen.the n and c terminals and tyrosine-phosphorylating site of the middle-sized tumor antigen of polyoma virus were chemically synthesized. the sequences of these peptides were met-asp-arg-val-leu-ser-arg-ala-asp-lys (n-mt), met-leu-phe-ile-leu-ile-lys-arg-ser-arg-his-phe (c-mt), and glu-glu-glu-glu-tyr-met-pro-met-glu (mt-tyr), respectively. among these peptides, the c-mt peptide inhibited phospholipase a2 (ec 3.1.1.4), phospholipase c (ec 3.1.4.3), and phospholipase d (ec 3.1.4.4). in addition, ph ...19852857079
porcine clostridium perfringens type a spores, enterotoxin and antibody to enterotoxin.forty-two clostridium perfringens type a strains isolated from cases of diarrhoea in pigs were tested for their ability to sporulate and produce enterotoxin in three different sporulation media. enterotoxin was produced by 11 of the 42 c perfringens type a isolates (26.2 per cent). thirteen isolates (30.9 per cent) produced spores at a frequency of 10 per cent or more. spore production was recorded in 24 (57.1 per cent) of the isolates. the titres of enterotoxin produced by the isolates ranged f ...19892547268
[ultrastructural changes in skeletal muscles in experimental gas gangrene with clostridium perfringens (author's transl)]. 19744138778
a review of pigbel (necrotising enteritis) in papua new guinea, 1961-1984.pigbel has been recognised as a major cause of mortality and morbidity in the papua new guinea highlands for over 20 years. the clinical features, epidemiology and pathogenesis of this disease have been elucidated, leading to the development of an effective vaccine (clostridium perfringens type c beta toxoid) for the prevention of pigbel.19852867654
entamoeba histolytica: concurrent irreversible loss of infectivity-pathogenicity and encystment potential after prolonged maintenance in axenic culture in vitro. 19734355413
[enterotoxemia in pigs and cattle due to clostridium welchii type a]. 19734357239
structural studies relating to the distribution of molecular components in erythrocyte membranes. 19744152595
the development of the flora of the alimentary tract in young animals. 19654285022
the ecology and epidemiology of the pig-bel syndrome in man in new guinea. 19664288244
two distinct toxic proteins in corynebacterium ulcerans. 19744370843
the abolition of the protective effect of clostridium welchii type a antiserum by ferric iron. 19674289804
[fatal experimental tetanus caused by a mixed infection with sublethal doses of perfrigens and tetanus spores]. 19664292019
[food poisoning caused by clostridium perfringens]. 19674294180
[toxin formation by cl. perfringens type e]. 19664307060
[peculiarities of fine structure and virus reproduction]. 19694310292
role of lecithin in the mechanism of tsh action. 19704312983
[use of antibiotics in combination with antiperfringens serum in the treatment of experimental gas infection caused by cl. perfringens in association with escherichia coli]. 19694314241
enzymes that destroy blood group specificity. i. purification and properties of alpha-l-fucosidase from clostridium perfringens. 19704314593
[immunization against gas gangrene and tetanus with sexta-anatoxin (in experiments with animals)]. 19694315881
[immunogenic properties of clostridium perfringens type a anatheta-hemolysin].guinea-pigs were immunized with anatheta-hemolysin preparations adsorbed on aluminum hydroxide, as well as with the mixture of anatheta-hemolysin and type a cl. perfringens toxoid, purified and concentrated. anatheta hemolysin preparations were obtained with the use of homogeneous theta hemolysin, as well theta hemolysin of various purification degrees. as a result, antatheta hemolytic guinea-pig sera capable of neutralizing 2,000-8,000 hu of theta-hemolysin were obtained. tests made to establis ...19816275640
role of bacteria in modifying virulence of entamoeba histolytica. studies of amebae from axenic cultures. 19704318244
association of clostridium perfringens type a with human diarrhoeal cases. 1977198362
erythemal activity of the cellular enteropathogenic factor of clostridium perfringens type a. 19704320760
[alpha-toxin (phospholipase c) of clostridium perfringens (author's transl)]. 1976194279
on the structure of the carbohydrate chains of blood group substances. 19704321410
effect of sulfatide and gangliosides on phospholipase c and phospholipase a2 activity. a monolayer study.the effect of sulfatide and gangliosides gm1, gd1a and gt1b on the activity of phospholipase c from clostridium perfringens on dilauroylphosphatidylcholine and of porcine pancreatic phospholipase a2 on dilauroylphosphatidic acid was studied in lipid monolayers containing different proportions of glycolipids under zero-order kinetics at various constant surface pressures. the presence of sulfatide in the monolayer increases the activity of phospholipase c at high surface pressures. gangliosides s ...19902378885
the role of enterotoxin in clostridium perfringens type a enteritis. 19714328690
epizootiologic investigations of a diarrheic syndrome in fattening pigs.a diarrheic syndrome linked to clostridium perfringens was observed in fattening pigs. a good correlation was observed between the onset and the severity of diarrhea and the fecal passage of c perfringens enterotoxin. intestinal fluids from affected pigs had activity comparable with that detected in a purified c perfringens enterotoxin. seven pigs excreting enterotoxin were shown to develop serum antibodies to c perfringens enterotoxin.19852865920
identification and typing of clostridia in raw milk in egypt. 19724346755
antigenic homogeneity of enterotoxin from different agglutinating serotypes of clostridium perfringens. 19734349468
electrostatic interactions in the binding of fluorescent probes to lipid membranes. 19734352075
the intestinal flora of pigs with special reference to clostridium perfringens. 19724353892
ultrastructural study of clostridial myositis. 19744358598
the pancreatic beta-cell recognition of insulin secretogogues. influence of neuraminidase treatment on the release of insulin and the islet content of insulin, sialic acid, and cyclic adenosine 3':5'-monophosphate. 19744369334
immunopurification of phospholipase c (alpha-toxin) from clostridium perfringens. 19744370089
bacterial adherence and biofilm formation on latex and silicone t-tubes in relation to bacterial contamination of bile.t-tube-related bacteriobilia causes infectious complications and obstruction. to prevent these, the choice of t-tube material may be of importance.19968726310
the interaction of phospholipase c from staphylococcus aureus and clostridium perfringens with cell membranes. 19744371133
necrotic enteritis in broiler chickens. ii. pathology and proposed pathogenesis.the intestines from 124 dead, sick and normal broiler chickens from 24 cases of necrotic enteritis were subjected to histological examination. tissue sections from the duodenum, jejunum, ileum and ceca from each broiler were examined histologically for lesions of necrotic enteritis and the presence of coccidia. lesions of necrotic enteritis were present in one or more areas of the intestine in all but six of 94 dead or sick birds and they were most common and severe in the jejunum. coccidia were ...19744373152
necrotizing infectious enteritis in piglets, caused by clostridium perfringens type c. ii. incidence and clinical features. 19675624909
clostridium perfringens type c causing necrotising enteritis.a rapidly fatal case of enteritis necroticans in a 24 year old man with diabetes was caused by clostridium perfringens type c. the role of beta toxin in the disease is discussed. this type has not been previously described as a causative agent in necrotising bowel disease of man outside endemic areas.19846088590
purification to homogeneity of h blood group beta-galactoside alpha 1 leads to 2 fucosyltransferase from porcine submaxillary gland.a beta-galactoside alpha 1 leads to 2 fucosyltransferase has been solubilized from porcine submaxillary glands and purified 124,000-fold to homogeneity by repeated affinity chromatography on gdp-hexanolamine agarose. sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified enzyme revealed two electrophoretic species with apparent mr = 60,000 and 55,000. the two enzyme species have not been completely resolved, but both appear to be active forms of the fucosyltransferase with app ...19806246105
radioiodination of enterotoxin from clostridium perfringens type a using chloramine t. 19806257632
purification and characterization of clostridium perfringens delta-toxin.delta-toxin, an extracellular hemolysin released by clostridium perfringens type c, was purified from culture supernatant fluid by sequential ammonium sulfate precipitation, thiol-sepharose gel chromatography, isoelectric focusing, and sephadex g-75 gel filtration. the purified preparation had a specific activity of 320,000 hemolytic units per mg of protein and was homogeneous, as determined by immunochemical and electrophoretic tests. this toxin was characterized as a single polypeptide chain c ...19816260669
survival of clostridial spores in animal tissues.spores injected intravenously into mice in numbers in excess of 10(2)/g of body weight were initially dispersed to most organs, but after a few days the remaining spores were concentrated in the liver, from which they were eliminated with a half-life of about 6 days. intraperitoneal injection did not result in contamination of organs unless initial spore numbers exceeded 10(5)/g of body weight, in which case the spores behaved in the same manner as those injected intravenously. oral administrati ...19816261688
studies on (k+ + h+)-atpase. iv. effects of phospholipase c treatment.(1) the total phospholipid content of a gradient purified (k+ + h+)-atpase preparation from pig gastric mucosa is 105 mumol per 100 mg protein, and consists of 29% sphingomyelin, 29% phosphatidylcholine, 28% phosphatidylethanolamine, 10% phosphatidylserine and 4% phosphatidylinositol. the cholesterol content corresponds to 50 mumol per 100 mg protein. (2) treatment with phospholipase c (from clostridium welchii and bacillus cereus) results in an immediate decrease of the phosphate content. up to ...19816272855
neuraminidase reduces the number of super-high-affinity [3h]oxotremorine-m binding sites in lung.the effects of neuraminidase on the binding of the radioligand agonist [3h]oxotremorine-m ([3h]oxo-m) were investigated in lung membranes. [3h]oxo-m labelled super-high-affinity binding sites (kd of 1.36 nm), as indicated by the very high affinity displayed by carbachol when tested in competition with 0.5 mm [3h]oxo-m. neuraminidase reduced the number of [3h]oxo-m binding sites with no change occurring in the kd. these results suggest that the effects of neuraminidase may explain virus-induced a ...19921612112
the detection of the factor lowering the immunogenicity of toxoid of clostridium perfringens type a. 19816280638
porcine sugar nucleotide: glycoprotein glycosyltransferases. 3. blood serum and liver n-acetylglucosaminyltransferase. 19714326781
electron microscopy of clostridium perfringens in the intestine of neonatal pigs with necrotic enteritis. 19852863412
the release of n-acetyl- and n-glycolloyl-neuraminic acid from soluble complex carbohydrates and erythrocytes by bacterial, viral and mammalian sialidases.a series of substrates, sialyl(2 leads to 6)galnac and ganglioside gm3, containing either n-acetylneuraminic acid (acneu) or n-glycolloylneuraminic acid (gcneu), has been prepared. the trisaccharide gcneu(2 leads to 3)lactose was preapred by ozonolysis of gcneu-gm3, and the disaccharides acneu(2 leads to 6)galnac and gcneu(2 leads to 6)galnac were isolated from bovine submandibular-gland mucin by alkali elimination. sialidases from newcastle-disease virus, fowl-plague virus, influenza virus a2, ...19817325957
isolation of nisin-producing lactococcus lactis wnc 20 strain from nham, a traditional thai fermented sausage.a total of 14,020 lactic acid bacteria (lab) were isolated from nham and screened for bacteriocin production. one lactococcus lactis strain wnc 20 produced a bacteriocin that not only inhibited closely related lab, but also some food-borne pathogens including listeria monocytogenes, clostridium perfringens, bacillus cereus and staphylococcus aureus. biochemical studies revealed that the bacteriocin was heat-stable even at autoclaving temperature (121 degrees c for 15 min) and was active over a w ...200312457588
a comparison of features and the microbial constitution of the fresh feces of pigs fed diets supplemented with or without dietary microbes.the features and the constitution of the microbial population of fresh feces were compared between pigs fed a diet supplemented with dietary microbes and pigs given nonsupplemented diets. the former were reared on farm c and the latter on farms a and b. the concentrations of ammonia-n, indole, and skatole of fresh feces were not significantly different between pigs reared on farm c and those raised on farms a and b, but the concentrations of ammonia-n and the skatole of fresh feces were signific ...199812501404
purification and biochemical properties of clostridium perfringens type a enterotoxin.the sporulation-specific enterotoxin of clostridium perfringens type a, which is the toxin active in human food poisoning, has been purified from extracts of sporulating cells. highly purified enterotoxin was obtained by treatment of crude cell extract with ribonuclease for 30 min, followed by sequential chromatography on sephadex g-100, cellex t cellulose, and hydroxylapatite. recovery was 65 to 75% of the initial activity. enterotoxin purity was > 99% as indicated by sedimentation velocity, se ...19724344364
phospholipids chiral at phosphorus. preparation and spectral properties of chiral thiophospholipids.the thiophospholipid 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) was shown to be a mixture of two diastereomers by 31p nuclear magnetic resonance. the isomer that resonates at the lower field in cdcl3 (56.12 ppm) was designated as isomer a and the other (resonates at 56.07 ppm) as isomer b. phospholipase a2 from four different sources (bee venom, naja naja venom, crotalus adamanteus venom, and porcine pancreas) was shown to hydrolyze the isomer b of dppsc specifically, whereas phosph ...19836688028
bacteriological analysis of uncooked pork sausages in relation to public health.one hundred and eighteen samples of uncooked pork sausages were analysed bacteriologically. the total aerobic plate count ranged from 1.8 x 10(8) to 9.2 x 10(8) cfu/g in the samples. high mean counts of staphylococci, enterococci, micrococci, bacillus cereus, salmonella and coliforms were observed. staphylococcus aureus were encountered with count range 2.8 x 10(6) cfu/g to 7.4 x 10(6) cfu/g. the viable count of escherichia coli ranged from 2.0 x 10(5) to 4.3 x 10(5) cfu/g. clostridium perfringe ...19947959987
the failure of antitoxin to protect guinea-pigs against intraperitoneal infection with clostridium welchii type a. 196314068944
[detection of botulism toxins and microbes of b and c types in the bodies of sick human and animal subjects and in cadavers]. 196314070208
[classification of histidine decarboxylating tissue and bacterial enzymes]. 196314077682
enterotoxemia in pigs due to clostridium perfringens type c. 196414173298
microbiological studies on hamburgers.one hundred and eighty-two raw, 112 pre-cooked and 750 cooked hamburgers composed mainly of beef or beef and pork were subjected to microbiological examination.raw hamburgers gave total bacterial counts from 10(6) to 10(8) per g, counts of enterobacteriaceae from 10(4) to 10(6) per g, of escherichia coli from 10(3) to 10(5), of group d streptococci from 10(2) to 10(4), of staphylococcus aureus from 3 to 10(2) and of clostridium perfringens less than 10 bacteria per g. of the samples, 32% contain ...19826276464
clostridial disease of the gut.clostridia are an important cause of morbidity and mortality in humans and animals. some of the most common clostridial infections are those of the gut. the primary infections in humans are clostridium perfringens food poisoning and clostridium difficile-mediated antibiotic-associated diarrhea and colitis. less common but important infections include non-food poisoning c. perfringens nosocomial diarrhea and c. perfringens type c necrotizing jejunitis (pig-bel). c. perfringens is also the dominan ...19957548565
duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools.a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ...200314605150
in vitro susceptibility of clostridium perfringens isolated from farm animals to growth-enhancing antibiotics.minimal inhibitory concentration (mic) determinations were carried out with seven growth-enhancing antibiotics against 95 clostridium perfringens field isolates obtained during 1991 and 1992 from poultry, pigs and calves. all were resistant to 64 micrograms ml-1 of the bambermycin antibiotic, flavomycin (flavophospholipol) and susceptible to avoparcin (mic90 0.25 micrograms ml-1), avilamycin (mic90 0.5 micrograms ml-1) and salinomycin (mic90 < or = 0.12 micrograms ml-1). acquired resistance agai ...19938365955
development of selected faecal microfloras and of phagocytic and killing capacity of neutrophils in young pigs.nine healthy piglets, weaned at the age of 35 days and emanating from two litters, were studied from 7 to 63 days of age. the development of their faecal flora was analysed by bacteriological quantification of coliforms, e. coli, enterococci and clostridium perfringens. the numbers of coliforms, e. coli and enterococci decreased from about 10(8) cfu/g faeces on day 7 to about 10(5) at the end of the study. clostridium perfringens, with an initial value of 10(4) cfu/g faeces, was not found in any ...19979100329
a hospital outbreak of clostridium perfringens food poisoning--implications for food hygiene review in hospitals.an outbreak of clostridium perfringens (c. perfringens) food poisoning affected 17 of 44 (38.6%) patients interviewed on two hospital wards. a case-control study showed a statistically significant association between the consumption of roast pork and illness (p < 0.01). c. perfringens type a, untypable serotype, was isolated from samples of pre-cooked vacuum sealed pork supplied by a local meat producer. faults were noted in the food production process at the factory. cuts of meat were too large ...19957738342
metabolism of 7-nitrobenz[a]anthracene by intestinal microflora.pure cultures of anaerobic intestinal bacteria and mixed fecal microflora from human, rat, mouse, and pig were screened for the ability to metabolize 7-nitrobenz[a]anthracene (7-no2ba). based on analysis by high-performance liquid chromatography (hplc) and by ultraviolet (uv), mass, and nuclear magnetic resonance (nmr) spectral techniques, the compounds were identified as 7-aminobenz[a]anthracene (7-nh2ba) and benz[a]anthracene 7,12-dione (dione). identification of 7-nh2ba as a metabolite of 7-n ...19947966445
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