[demonstration of yersinia enterocolitica in samples of feces from slaughter pigs]. 1979473979
[yersinia enterocolitica in acute appendicitis, lymphadenitis mesenterialis and regional enteritis (author's transl)].in 895 patients operated on for acute appendicitis we found yersinia enterocolitica in 41 cases. another two cases were detected in postoperative serological examinations. lymphadenitis mesenterialis was present in 17 cases during operation. once we detected a regional enteritis. histological examination of the appendix revealed a purulent inflammation in 21 cases and a phlegmonous-gangrenous inflammation in 4 cases. the other 18 patients suffered from oxyuriasis, coprostasis or scars of the app ...1976961157
frequency of isolation of yersinia enterocolitica from livestock in trinidad. 19921300681
[the boosting of yersinia enterocolitica virulence during passage through infusorians and mammalian macrophages (comparative research)].selective changes in y. enterocolitica virulence have been made in experiments on eukaryotic cells, infusoria, and peritoneal macrophages, used as models. in parallel experiments with the passage of yersinia through infusoria and mammalian macrophages an analogy to the processes of phagocytosis has been established.19911950257
new selective agar medium for isolation of virulent yersinia enterocolitica.a selective agar medium for isolation of virulent yersinia enterocolitica (vye agar) was developed for the rapid and accurate isolation of virulent y. enterocolitica from environmental samples highly contaminated with environmental yersinia organisms, as well as for isolation from clinical specimens. vye agar provided a quantitative recovery of 51 different strains of virulent y. enterocolitica at 32 degrees c after incubation for 24 h. the cefsulodin, irgasan, josamycin, and oleandomycin conten ...19873597750
yersinia enterocolitica infection in humans and isolation of the microorganism from pigs in japan. 19873665513
pathogenicity of yersinia enterocolitica biotype 3b and 4, serotype 0:3 isolates from pork samples and humans. 19873665510
isolation of yersinia enterocolitica from the faeces of diarrhoeic april and may 1983, a total of 124 samples of faeces from 124 swine was examined for the presence of yersinia enterocolitica by a variety of isolation procedures. the organisms were recovered from 48.4% of the swine, which all had diarrhoea. the strain belonged to serotypes 0:3 and 0:9 which are considered to be pathogenic for humans.19873624105
new enrichment method for isolation of pathogenic yersinia enterocolitica serogroup o:3 from pork.a new enrichment medium for the recovery of pathogenic yersinia enterocolitica serogroup o:3 from naturally infected meat products based on three selective agents, irgasan, ticarcillin, and potassium chlorate (itc), was compared with several other one- or two-step enrichments. y. enterocolitica serogroup o:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. itc was by far the most sensitive method for the recovery o ...19883377499
yersinia enterocolitica serotype 03 in naturally infected raw pork. 19863959377
isolation of campylobacter spp. and yersinia enterocolitica from domestic animals and human patients in kenya.rectal swabs or faecal samples from 992 domestic animals and 97 human patients in the nairobi region were cultured for thermophilic campylobacter species and yersinia enterocolitica. the highest isolation rate of campylobacters was obtained from diarrhoeic pigs (55.1%), followed by healthy chicken (51.5%), diarrhoeic dogs (47.2%), healthy pigs (44.0%), healthy ducks (29.4%), healthy goats (6.3%), healthy cattle (5.8%), diarrhoeic humans (3.1%), and healthy sheep (2.0%). only one strain of y. ent ...19883345259
[campylobacter jejuni infections in slaughterhouse workers].complement fixing (c.f.) antibodies to campylobacter jejuni were detected in 83 slaughterhouse workers and 83 blood donors. workers were aged 18-65 years (mean, 41.7 +/- 12.3) and had worked in the slaughterhouse for 2-40 years (mean, 17.5 +/- 5.1). c.f. antibodies were detected according to mosimann's method and by including five antigens: campylobacter jejuni, yersinia enterocolitica types 03 and 09, yersinia pseudotuberculosis and brucella. positive titers were found in 12.1% of workers and i ...19883154982
isolation of yersinia enterocolitica and related species from foods in france.the occurrence of yersinia enterocolitica and related species (y. intermedia, y. frederiksenii, y. kristensenii) in foods from france was investigated by using different enrichment procedures. initially, seven procedures were evaluated with pork products. these methods included a cold preenrichment in yeast extract-rose bengal broth or in phosphate-sorbitol-bile medium, followed by selective enrichment either in pastone-sucrose-tris-azide broth, in modified rappaport broth, or in bile-oxalate-so ...19854083878
restriction endonuclease analysis of 40- to 50-mdalton plasmids from yersinia enterocolitica strains of worldwide origin. 19872822354
studies on yersinia enterocolitica. i. isolation of y. enterocolitica from swine. 19734800056
microbiological studies on hundred and eighty-two raw, 112 pre-cooked and 750 cooked hamburgers composed mainly of beef or beef and pork were subjected to microbiological examination.raw hamburgers gave total bacterial counts from 10(6) to 10(8) per g, counts of enterobacteriaceae from 10(4) to 10(6) per g, of escherichia coli from 10(3) to 10(5), of group d streptococci from 10(2) to 10(4), of staphylococcus aureus from 3 to 10(2) and of clostridium perfringens less than 10 bacteria per g. of the samples, 32% contain ...19826276464
the prevalence of yersinia enterocolitica 0:3 in finnish pigs and hundred and forty seven samples of pig faeces collected from 14 herds located in different parts of finland were examined for yersinia enterocolitica biotype 4 serotype 0:3. twenty six (17.7%) animals and 5 herds (35.7%) were positive. the tonsils of 350 animals from 35 herds with high condemnation figures at meat inspection and the tonsils of 131 animals from 13 herds with low condemnation figures collected from 2 abattoirs in southwest finland were also examined. the prevalence raes of y.e ...19902399870
yersinia enterocolitica o:3 infections in infants and children, associated with the household preparation of chitterlings. 19902314448
a synthetic oligonucleotide probe and a cloned polynucleotide probe based on the yopa gene for detection and enumeration of virulent yersinia enterocolitica.we compared a synthetically produced 19-mer oligonucleotide probe with a polynucleotide probe consisting of a cloned fragment of the virulence gene yopa for their relative efficiencies in identification and enumeration of virulent yersinia enterocolitica. the probes were used in dna-dna colony hybridization assays to differentiate 70 yersinia strains with known plasmid profiles. all 19 strains harboring the 40- to 50-megadalton virulence plasmid were positive in the hybridization assay, whereas ...19902310179
occurrence of yersinia enterocolitica in pork products from northern taiwan. 19911935115
aspects of the epidemiology of yersinia enterocolitica: a review.a review of works concerning different aspects of the epidemiology of human pathogenic yersinia enterocolitica biogroup iv/serogroup o:3 (y. enterocolitica o:3) is given. to investigate the epidemiology of y. enterocolitica o:3 in danish herds of pigs, tonsil swabs from 2218 freshly slaughtered pigs originating from 99 herds were examined. the organism was isolated from 25% of the pigs and from 82% of the herds. no herd management factor could be associated with the presence of the organism. the ...19911892741
first isolation of yersinia enterocolitica serotype o:8 in japan.a 4-year-old japanese boy was infected with yersinia enterocolitica serotype o:8, h:befiv, biotype 1b, phage type xz, restriction endonuclease analysis of plasmid dna type b, restriction endonuclease analysis of chromosomal dna type 8. he presented with acute gastroenteritis with elevated body temperature (40 degrees c), rigor, and shivers. the diagnosis was septicemia. this is apparently the first report of this serotype from a human infection outside north america.19911812854
application of restriction endonuclease analysis and genetic probes in the epidemiology of yersinia enterocolitica infection. 19911657520
yersinia enterocolitica. 19827050068
[comparison of yersinia enterocolitica strains isolated from swine and humans in guipuzcoa].yersinia enterocolitica biotype 4, serotype 3 is a pathogenic bacteria for men. its most likely reservoir are pigs. we have compared 48 y. enterocolitica serotype 0:3 strains isolated from pig's tonsils samples as well as porcine-derived food with 301 y. enterocolitica serotype 0:3 isolate from human stool's samples. all strains are positive for virulence tests. yersinia enterocolitica serotype 0:3, biotype 4, phage-type viii was nearly the only single pathogenic strain isolated from both origin ...19921606219
laboratory investigation of virulence among strains of yersinia enterocolitica and related species isolated from pigs and pork products.eighty strains of yersinia enterocolitica and related species isolated from slaughtered pigs and pork products were tested for possession of virulence-associated phenotypes by employing 12 in vivo and in vitro assays. the isolates could be broadly divided into two groups: (i) strains belonging to pathogenic bioserotypes of y. enterocolitica that displayed virulence-associated characteristics in most or all assays and (ii) strains belonging to y. enterocolitica biotype 1a and to related species t ...19921521193
possible confounders of the relationship between occupational swine contact and yersinia enterocolitica 0:3 and 0:9 antibodies.increased risk of high yersinia enterocolitica 0:3 and 0:9 antibody content has been previously reported in occupations with swine contact. in this study several possible confounders of this elevated risk among pig farmers and abattoir workers were considered. only in three instances the standardized risk ratio was decreased close to unity, namely after the standardization of y. enterocolitica 0:3 igg antibody positivity for age among abattoir workers, after the standardization of y. enterocolit ...19921397221
biotypes and sensitivity screening yersinia enterocolitica as an infective agent in man and swine in nigeria.diarrhoeic faecal samples from 210 humans and 192 swine were screened for yersinia enterocolitica in 1990. ten and 8 y. enterocolitica strains were isolated from pig and man, respectively. the isolates were found to belong to wauter's biotypes 1, 2, 3 and 4. biotype 2 was isolated mainly from human stool samples. biotype 3 was found only in swine while biotypes 1 and 4 were isolated from both man and swine. all the 18 strains showed varying degrees of sensitivity to antibiotics used in this inve ...19921301624
isolation of yersinia enterocolitica from some animals and meats. 19751237747
[fertility problems caused by infectious agents pigs in the netherlands(author's transl)].the concept of fertility problems is defined in the introduction and their economic importance in pig breeding are pointed out. the infectious causes of the problem are reviewed. infections with l. tarassovi and with aujezky's virus regularly lead to practically endemic abortions; brucella suis and swine-plague infections do so sporadically. the smedi syndrom is considered. the clinical symptoms, diagnosis, epizootiology, and the therapeutical and prophylactic measures of these infections are di ...19751172635
[bacteriocinogeny in yersinia enterocolitica].bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ...19751091204
bacteriological analysis of uncooked pork sausages in relation to public hundred and eighteen samples of uncooked pork sausages were analysed bacteriologically. the total aerobic plate count ranged from 1.8 x 10(8) to 9.2 x 10(8) cfu/g in the samples. high mean counts of staphylococci, enterococci, micrococci, bacillus cereus, salmonella and coliforms were observed. staphylococcus aureus were encountered with count range 2.8 x 10(6) cfu/g to 7.4 x 10(6) cfu/g. the viable count of escherichia coli ranged from 2.0 x 10(5) to 4.3 x 10(5) cfu/g. clostridium perfringe ...19947959987
isolation of yersinia enterocolitica from danish swine and dogs.seventeen strains of yersinia enterocolitica were isolated on examination of the caecal contents from 100 bacon pigs at slaughter. in another material consisting of 222 pigs with various diseases, the bacterium was found in 12 cases. three out of 40 dogs were positive for the bacterium. seven of the porcine and one of the canine strains belonged to serotype o 3, which is a human pathogen.1976970137
two-year study of endemic enteric pathogens associated with acute diarrhea in new caledonia.a longitudinal study of diarrheal disease among patients of all ages with acute diarrhea was carried out in new caledonia from january 1990 to december 1991. stool samples from 2,088 diarrheal patients were examined for parasites, rotavirus, and bacterial pathogens. potential sources of contamination (drinking water, seawater and bovine and porcine feces) were investigated. one or more enteric pathogens were identified in 41.8 and 40.6% of the persons with diarrhea, in 1990 and 1991, respectivel ...19948077399
yersinia enterocolitica: a significant 'new' thrives in the cold, defies conventional culture methods, does not respond to most antibiotics, and may mimic other disorders in its manifestations. fortunately, most cases of yersinial enterocolitis are mild and self-limiting, but infection can be life-threatening, especially in neonates or when immunologic defenses are impaired. clinical and laboratory diagnostic criteria and epidemiologic clues are described.1978730137
selective isolation from hela cell lines of yersinia pseudotuberculosis, pathogenic y. enterocolitica and enteroinvasive escherichia coli.using hela cell lines, we obtained an optimal and selective isolation of yersinia pseudotuberculosis, pathogenic y. enterocolitica and enteroinvasive escherichia coli from samples such as pork, feces and river water heavily contaminated with other bacteria.19948167427
comments on the ecology and epidemiology of yersinia enterocolitica in czechoslovakia.on the basis of the information disseminated by the reference laboratory in 1968, the microbiological diagnosis of yersinia enterocolitica (ye) was gradually mastered by all czechoslovak microbiological laboratories. the most frequent serotype isolated in man was ye 03. isolation predominated from cases of diarrhoea, less often from appendectomies, and only one case of septicaemia was described. usually only sporadic cases occur and outbreaks are rare. a few strains of ye 05 were found in diarrh ...1979535370
inhibition of yersinia enterocolitica serotype o3 by natural microflora of pork.yersinia enterocolitica serotype o3 did not grow but did survive in inoculated raw ground pork kept at 6 and 25 degrees c. the antagonistic effect of microbial flora, especially hafnia alvei and environmental yersinia organisms, on the growth of y. enterocolitica serotype o3 in raw ground pork was evident. these results were supported by evidence of the inhibition of growth of y. enterocolitica serotype o3 by enterobacteriaceae, especially h. alvei and environmental yersinia organisms, in mixed ...19863729395
comparison of three molecular methods for typing and subtyping pathogenic yersinia enterocolitica strains.the efficiency of pulsed-field gel electrophoresis (pfge), ribotyping and restriction enzyme analysis of the virulence plasmid (reap) for typing and subtyping strains of yersinia enterocolitica was compared. all three techniques gave concordant results, and the strains studied could be separated into three distinct clusters: (1) heterogeneous strains of biotype 1a and serotype o5 (1a/o5); (2) one 3/o3 strain and all 2/o9 strains; and (3) all 4/o3, 2/o5 and two 3/o3 strains. within cluster 3, the ...19968667412
yersinia enterocolitica biotype 3b serotype 03 phage type ii infection in pigs. 19854094268
[campylobacter jejuni, yersinia enterocolitica and salmonella in game and poultry].samples of various sorts of game and poultry (other than chicken), obtained from poulterers, hunters, rearing-farms and slaughterhouses, were surveyed for the presence of campylobacter jejuni, yersinia enterocolitica and salmonella. these pathogens were isolated from hare, wild boars, pheasants, guinea-fowl, turkeys, mallards and domestic ducks. yersinia enterocolitica and salmonella were also cultured from wild and domestic rabbits. moreover, yersinia enterocolitica was isolated from wood pigeo ...19836648946
detection of pathogenic yersinia enterocolitica by polymerase chain reaction and digoxigenin-labeled polynucleotide probes.yersinia enterocolitica is widespread in nature, but only a few bioserotypes are involved in human infections. pigs are considered to be the major reservoirs of pathogenic strains. it is essential to have an accurate and rapid method for the detection of pathogenic yersiniae. to achieve this objective, 19-base synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) to detect the ail gene (which is conserved only in pathogenic strains) in strains of y. enterocolitica and ...19921400967
isolation of yersinia enterocolitica strains from pigs and wild boars. 19854074519
[seasonal isolation of yersinia enterocolitica and yersinia pseudotuberculosis from tonsils of healthy slaughter pigs (author's transl)].45 strains of yersinia (y.) enterocolitica from which 36 respectively 9 belonged to o-group i or v and 28 strains of y. pseudotuberculosis from which 15 respectively 13 isolates belonged to serotype i or ii were isolated from 70 tonsils of 480 examined, clinically healthy slaughter pigs. in the tonsils of three animals were found both species together. y. enterocolitica was isolated during the whole year, but especially in the months of november (17.5%) and december (32.5%). y. pseudotuberculosi ...19817198363
[cultural and serological studies of the occurrence of yersinia enterocolitica infections in swine and cattle]. 19817316949
[atypical routes for penetration of the infection in intestinal yersinioses. iii. biochemical changes in alveolar macrophages in aerosol infections with yersinia enterocolitica]. 19846741620
pathogenicity of yersinia enterocolitica serotype o3 biotype 3 is known that yersinia enterocolitica infection in japan is caused mainly by serotype o3 biotype 4 strains. recently, however, a number of serotype o3 strains which were classified biotype 3 and which ferment lactose and xylose, instead of sorbose, and give a negative voges-proskauer reaction have been isolated from both humans and animals. in this study, comparisons of four properties were made among isolates of y. enterocolitica serotype o3 biotype 3 from humans, pigs, dogs, cats, and rats ...19873818938
introduction into japan of pathogenic yersinia through imported pork, beef and fowl.yersinia was isolated from imported raw meat and fowl products by hela cell treatment and conventional koh-treatment, to obtain information on the origin of pathogenic yersinia in japan. forty-one strains of yersinia enterocolitica and one strain of yersinia pseudotuberculosis, serotype 4b were isolated from 38 (3.0%) of 1278 samples of pork, two (0.3%) of 612 samples of beef and two (0.3%) of 615 samples of chicken. y. enterocolitica isolates belonged to b:4/o:3 (biotype/serotype, 15 strains), ...19979105929
[yersinia enterocolitica. study of its pathogenic characteristics]. 19854024512
[yersinia enterocolitica as a possible causative agent in food poisoning]. 19846382797
[isolation and characterization of yersinia enterocolitica from pigs and bovines in chile].the occurrence of yersinia enterocolitica in tonsils and rectal swabs from 100 healthy pigs and the rectal swabs of 100 healthy cattle slaughtered at santiago-chile were analysed. yersinia enterocolitica was isolated from 48 (48%) pigs but not from cattle. 98.2% of strains were of 4/o3 bioserogroup, considered to be pathogenic for humans. all of the strains were resistant to penicillin producing beta-lactamase. most of them were resistant to neomicin and tetracycline. the pyv marker was used to ...19979312970
characterization of yersinia enterocolitica isolated from the oral cavity of swine in slovakia.yersinia enterocolitica was detected in 160 of 2760 examined samples that were prepared from dental cavity of 920 pigs. from the isolates 49 strains were characterized with respect to their genetic and phenotypic markers of virulence. all strains were belonging to serogroup 0:3, biovar 4. forty-one isolates (84%) harboured the virulence plasmid detected by dna colony hybridization. of the other assays of virulence--autoagglutination, binding of crystal violet, pyrazinamidase activity, calcium de ...19957710919
surveillance of antimicrobial resistance in bacteria isolated from food animals to antimicrobial growth promoters and related therapeutic agents in denmark.this study was conducted to describe the occurrence of acquired resistance to antimicrobials used for growth promotion among bacteria isolated from swine, cattle and poultry in denmark. resistance to structurally related therapeutic agents was also examined. three categories of bacteria were tested: 1) indicator bacteria (escherichia coli, enterococcus faecalis, enterococcus faecium), 2) zoonotic bacteria (campylobacter, salmonella, yersinia enterocolitica), and 3) animal pathogens (e. coli, sta ...19989725794
enterotoxin production by yersinia enterocolitica in food samples.milk and cold meat samples were contaminated with 7 various serogroups of yersinia enterocolitica strains. the infected food samples were incubated under different conditions of growth, at different temperatures and for different periods of time, then the number of colony forming units was determined and enterotoxin production was assayed by the suckling mice test. the y. enterocolitica strains multiplied well under varying conditions of growth, but enterotoxin production could be detected only ...19846540029
an aroa mutant of yersinia pestis is attenuated in guinea-pigs, but virulent in mice.this study describes a pcr-based approach for the production of a rationally attenuated mutant of yersinia pestis. degenerate primers were used to amplify a fragment encoding 91.45% of the aroa gene of y. pestis mp6 which was cloned into puc18. the remainder of the gene was isolated by inverse pcr. the gene was sequenced and a restriction map was generated. the y. pestis aroa gene had 75.9% identity with the aroa gene of yersinia enterocolitica. the cloned gene was inactivated in vitro and reint ...19968757748
[yersinia enterocolitica serovar 2a, wb, 3:b,c biovar 5 in hares and sheep].during the last fourty years the "hare type" of yersinia enterocolitica was detected in the brown hare and other mammals in several european countries. in northern germany (province of schleswig-holstein) the pathogen was not found in the post mortem materials of the cattle, swine and horses during the years 1990-1996. in approximately 1,000 sheep studied this yersinia was cultured from the internal organs of one diarrhoeic lamb. the examination of some 200 perished hares reveals one animal with ...19979290039
detection of yersinia enterocolitica serogroup o:3 using different inoculation methods of the enrichment medium iragasan ticarcillin chlorate. 19958833813
risk factors for sporadic yersinia enterocolitica infections in norway: a case-control study. 19958833788
direct detection and isolation of plasmid-bearing virulent serotypes of yersinia enterocolitica from various foods.a procedure was developed for direct detection, isolation, and maintenance of plasmid-bearing virulent serotypes of yersinia enterocolitica from different food sources. plasmid-bearing virulent strains of y. enterocolitica representing five serotypes were simultaneously detected and isolated from enriched swab samples of artificially contaminated pork chops, ground pork, cheese, and zucchini, using congo red binding and low-calcium-response tests. the method was also effective in isolating plasm ...19979406416
ecological studies of yersinia enterocolitica. iii. cross-protection against fecal excretion between y. enterocolitica serovars 3 and 5.27 in pigs.yersinia enterocolitica biovar 4 serovar 3 and biovar 2 serovar 5.27 failed to establish in the intestines of pigs when challenged with the homologous or heterologous strains. after inoculation, the serum o-agglutinin titers were 1/10 or less and were not boosted by challenge with either serovar. y. enterocolitica were not recovered in any specimens at the time of slaughter.19846495608
integrating microbial decontamination with organic acids in haccp programmes for muscle foods: prospects and controversies.a considerable literature reports the antibacterial efficacy of dilute solutions of organic acids (lactic, acetic). with carcasses an overall reduction in surface contaminants of 1.5 log cycles can be expected. carcass decontamination may not improve the safety of the resultant meat, but laboratory trials confirm that acid decontamination of subprimal and retail cuts is more efficacious. an advantage over many other intervention strategies is that residual antimicrobial activity is demonstrable ...19989851597
prophylactic measures in order to reduce contamination of pig carcasses with yersinia enterocolitica during slaughter. 19958833798
a longitudinal study on enteropathogenic infections of livestock in trinidad.a longitudinal study was conducted on selected livestock farms to determine the prevalence of enteropathogens in diarrhoeic and non-diarrhoeic animals. the enteropathogens assayed from faecal samples and rectal swabs were bacteria (escherichia coli, campylobacter spp. salmonella spp. and yersinia enterocolitica), parasites (coccidia, gastrointestinal nematodes and cryptosporidium spp.) and viruses (group a rotavirus and parvovirus). the prevalence of the enteropathogens in various animal species ...200111340494
arsenite-induced multiple antibiotic resistance phenotype in environmental isolates of yersinia enterocolitica.minimal inhibitory concentrations (mics) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. for y. enterocolitica isolates obtained from wastewater (sewage effluents), an unequivocal increase in mics was seen with amikacin and gentamicin. no change was discernible in the outer-membrane proteins after isolates were grown ...200111391480
isolation of pathogenic yersiniae from wild animals in bulgaria.pathogenic yersinia strains were isolated between december 1998 and april 1999 from 37 wild animals: rabbit (lepus europeus), boar (sus scrofa scrofa), asiatic jackal (canis aureus), red fox (vulpes vulpes), mouflon (ovis musimon), european river otter (lutra lutra), beech marten (martes foina), polecat (musleta putorius) and wild cat (felis silvestris). it was established that among the wild animals y. enterocolitica strains of serotype 0:3 predominated, accompanied by y. pseudotuberculosis str ...200111393816
time course of the serological response to yersinia enterocolitica o:3 in experimentally infected pigs.a total of 25 pigs inoculated with yersinia enterocolitica serovar o:3 and 25 un-inoculated controls were followed weekly by sampling blood and faeces for 70 days post infection (p.i.). all inoculated pigs were faeces culture positive from day 5 to 21 p.i., whereafter shedding of bacteria declined to < 10% of the pigs at day 49 p.i. and to 0% at day 68 p.i. all control pigs remained y. enterocolitica o:3 culture negative. when examined in an indirect elisa using purified lps from y. enterocoliti ...19969054125
isolation of yersinia enterocolitica and related species from porcine samples obtained from an abattoir.swabs of swine carcasses and samples of porcine tongue and trim obtained from an abattoir were examined for the presence of yersinia enterocolitica and related species (y. intermedia, y. kristensenii and y. frederiksenii). three enrichment media (phosphate buffered saline, sorbitol-bile salts-phosphate buffered saline, and a modified rappaport's broth) were compared at 4 degrees c for their efficiency of recovery of y. enterocolitica and related species. two secondary enrichment procedures (post ...19846746463
[brucella titers in swine caused by yersinia enterocolitica, serotype 0:9].antibody to brucella abortus developed in two thirds of all gilts kept on a pig breeding station. systematic tests taken for the purpose of detecting clinical symptoms and of isolating brucella were negative. however, yersinia (y.) enterocolitica, serotype 0:9, was cultured from rectal swab samples which had been obtained from 31 to 78 gilts. the clinical, bacteriological, and serological tests gave rise to the assumption that the brucella titres have been caused by yersinia enterocolitica infec ...19806779771
detection of pathogenic yersinia enterocolitica and yersinia pseudotuberculosis from pork using the polymerase chain reaction. 19958833821
[demonstration of yersinia enterocolitica and yersinia pseudotuberculosis in fecal samples of healthy slaughter swine depending on the season]. 19817324640
[detection of escherichia coli strains harboring pathogenicity island of yersinia enterolitica in diarrheal patients and animals in china].to investigate the prevalence of escherichia coil strains harboring high pathogenicity island of yersinia enterocolitica in diarrhea patients, animals, food samples and resulted clinical symptoms.200011860774
[survey of the presence of bacterial pathogens in foods sold at retail stores in the city of cassino].the presence of bacterial food pathogens was evaluated in 154 food samples collected from supermarkets and butchers in the city of cassino (south-central italy). food pathogens were identified in 17.5% of the total food samples. in the raw meat samples, 24.6% tested positives for listeria monocytogenes, 4.3% for salmonella and 2.9% for escherichia coli o157. y. enterocolitica, only investigated in pork meat, was identified in 7.4% of the samples. in poultry, l. monocytogenes was identified in 55 ...200212070904
detection of yersinia enterocolitica o:3 specific antibodies in experimentally infected pigs by an indirect lps elisa. 19958833811
anticipatory guidance and chitterlings. 19989821437
prevalence of bacterial faecal pathogens in separated and unseparated stored pig examine the prevalence and diversity of bacterial faecal pathogens in unseparated slurry, separated solids and liquid fractions from a commercial pig farm.200312641712
application of yersinia kristensenii bacteriocin as a specific marker for the rapid identification of suspected isolates of yersinia enterocolitica.out of 143 suspected foods and clinical isolates of the genus yersinia, 77 isolates were bacteriocin-sensitive and 66 bacteriocin non-sensitive. of the 77 bacteriocin-sensitive isolates, 72 (93.5%) were identified as y. enterocolitica and 5 (6.49%) as y. frederiksenii. out of 66 bacteriocin non-sensitive suspected isolates, 22 (33.3%) were identified as yersinia spp. other than y. enterocolitica and 43 (65.1%) were other gram-negative bacteria with the exception of one (1.5%) isolate identified ...19957766076
reduction of yersinia enterocolitica and listeria spp. on pig carcasses by enclosure of the rectum during sealing off the rectum with a plastic bag immediately after it had been freed, the spread of y. enterocolitica o:3/biovar 4 to pig carcasses could be considerably reduced. the organism was recovered from only 0.8% of carcasses when the plastic bag technique was employed. y. enterocolitica o:3/biovar 4 was recovered from 10% of pig carcasses when eviscerating procedures did not include the use of the plastic bag technique. there was thus an obvious risk of the bacteria further contaminating me ...19947848781
short-comings of irgasan ticarcillin chlorate broth for the enrichment of yersinia enterocolitica biotype 2, serotype 9 from meat.recovery of yersinia enterocolitica serotype 0:9 from artificially contaminated minced pork with the enrichment medium irgasan ticarcillin chlorate broth (itc) was poor. this was due to the lower growth rate of this serotype in comparison with serotype 0:3. tests on the behaviour of serotype 0:3 and 0:9 towards some selective agents in itc broth showed that the minimal inhibitory concentration of chlorate was much lower for serotype 0:9 than for serotype 0:3. omitting chlorate in the itc enrichm ...19947848786
duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools.a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ...200314605150
[specific pathogen-free health programs].spf programs have been established in various countries, particularly in denmark and switzerland these programs are already well advanced. the system proves to be advantageous for export dealings but also with regard to public health. dalland is an internationally operating dutch pig breeding organisation. in 1989 a spf nucleus breeding farm was established in north-east france. a special program was set up for the supply of animals from this spf nucleus. in this paper it is concluded that spf c ...19948036609
development of a l-rhamnose and d-arabitol supplemented macconkey agar to identify pathogenic yersinia enterocolitica among environmental yersinias in swine production wastes.supplemented macconkey agar with l-rhamnose and d-arabitol distinguishes pathogenic from environmental strains of yersinia enterocolitica recovered from swine production wastes. this medium has a sensitivity of 100% and a specificity of 97.4%.200415063070
yersinia enterocolitica infection in a patient with sickle cell disease after exposure to chitterlings.we describe certain clinical, epidemiologic, and host-susceptibility features of yersinia enterocolitica infection in the context of a patient with underlying risk factors.19948166368
bacteria as dna vaccine carriers for genetic immunization.genetic immunization with plasmid dna vaccines has proven to be a promising tool in conferring protective immunity in various experimental animal models of infectious diseases or tumors. recent research focuses on the use of bacteria, in particular enteroinvasive species, as effective carriers for dna vaccines. attenuated strains of shigella flexneri, salmonella spp., yersinia enterocolitica or listeria monocytogenes have shown to be attractive candidates to target dna vaccines to immunological ...200415532991
immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kda protein is related to the mycobacterial 65 kda heat shock protein (hsp-65).smooth brucella spp. share certain lipopolysaccharide antigens with other bacteria, resulting in serological cross-reactions which can prevent the definitive diagnosis of brucellosis. to identify other antigens with serodiagnostic potential, immunoblot studies following sodium dodecyl sulphate-polyacrylamide gel electrophoresis were carried out. sera from pigs experimentally infected with brucella suis and naturally infected feral pigs, sera from pigs from a farm with a known history of yersinia ...19948203127
differentiation of yersinia enterocolitica serotype o:5,27 strains by phenotypic and molecular techniques.restriction endonuclease analyses of virulence plasmid dna (reap) and chromosomal dna and other phenotypic characteristics were used to study the differentiation of yersinia enterocolitica serotype o:5,27 strains. there was a close correlation between reap patterns and the geographical distribution of serotype o:5,27. human isolates produced only one reap pattern, which was also found with isolates from pigs and dogs.19938391027
[the absence of yersinia enterocolitica in foods and animal reservoirs in areas of the state of pernambuco, brazil].a search for the presence of enteropathogenic bacteria in fresh vegetables obtained in 5 restaurants from the city of recife, revealed neither yersinia enterocolitica nor other pathogenic bacteria in 96 samples analyzed. furthermore, y. enterocolitica was not found in the oral and rectal swabs taken from 15 apparently healthy pigs at an abattoir in the municipality of bonito in the pernambuco state. another search in which twenty one rodents from four species and one marsupial specimen were exam ...19979273565
comparison of sampling and isolation procedures for recovery of yersinia enterocolitica serotype o:3 from the oral cavity of slaughter pigs. 19853898776
control of yersinia enterocolitica in pigs at herd level.a higher herd prevalence of antibodies (elisa) to yersinia enterocolitica o:3 was found in conventional slaughter production (86.0% seropositive herds) than in conventional farrow-to-finish herds (53.1% seropositive herds). the herd prevalence of antibodies to y. enterocolitica in multiplying herds (56.1%) was similar to the level in the conventional farrow-to-finish herds. an epidemiological study in conventional pig herds demonstrated that farrow-to-finish production (odds ratio, or = 0.15) wa ...19989926996
evaluation of culture methods for rapid screening of swine faecal samples for yersinia enterocolitica o:3/biotype two studies, seven different culture protocols were compared to test naturally contaminated faecal samples from pigs for isolation of y. enterocolitica serotype o:3/biotype 4 (n = 70 and n = 79). four of the protocols were based on the nordic committee on food analysis (nmkl, protocols), while three protocols were based on a rapid and selective method (here called itc protocols). the protocols differed mainly in time of pre-enrichment (1, 10 and 24 d) and enrichment (2, 10, 24 d) and the type ...199910337241
yersinia enterocolitica: overview and epidemiologic correlates.yersinia enterocolitica comprises both pathogenic and nonpathogenic members. distinguished by biogrouping, serogrouping, and ecological distribution, commonly occurring pathogenic serobiogroups, e.g., o:3/4; o:5,27/2; o:8/1b; o:9/2, possess both chromosomal and plasmid-mediated virulence traits. studies have revealed several (oral, blood transfusion) modes of acquisition, elucidated the putative role of chromosomal and plasmid-encoded virulence factors in the pathogenesis of human infection, and ...199910602666
[what happens with yersinia in agriculture?]. 200010895609
isolation of yersinia enterocolitica from raw milk and pork in ludhiana. 200516758701
note: isolation, characterization and epidemiology of yersinia enterocolitica from humans and animals.during an 11-year period (1983 to 1994), 51 strains of yersinia enterocolitica were isolated from humans and animals. specimens were collected from a total of 3601 sources consisting of 956 patients with enteritis, 300 patients with urinary tract infection, 1564 healthy humans, 510 swine, 38 guinea-pigs, 118 rats and 115 rabbits. five strains of y. enterocolitica, bio/serogroups 2/o:9 and 4/o:3, virulence positive, were recovered from patients. forty-two variants of y. enterocolitica belonging t ...19968972095
yersiniosis in zoo marmosets (callitrix jacchuss) caused by yersinia enterocolitica 4/o:3.the aim of this research was to describe two fatal cases of yersinia enterocolitica bioserotype 4/o:3 infection in non-human primates and to characterise the isolates by pcr and pfge. in july 2004, two marmosets (callitrix jacchuss) born in captivity in zagreb zoo, died following a few days of intermittent diarrhoea in intervals of 2 weeks. the pathomorphological diagnosis of the female (born in 1997) and the male (born in 1995) marmoset, was disseminated miliary necrosis of the liver. y. entero ...200717223285
degradation of n-acyl-l-homoserine lactones by bacillus cereus in culture media and pork extract.degradation of the quorum-sensing signal molecule n-acyl-l-homoserine lactone (ahl) in cocultures was verified with bacillus cereus and yersinia enterocolitica in culture medium and in pork extract. results showed evidence of microbial interaction when the ahl-degrading bacterium and ahl-producing bacterium were cocultured in a food-simulating condition.200717293532
occurrence of pathogenic yersinia enterocolitica in norwegian pork products determined by a pcr method and a traditional culturing method.from october 1997 to april 1998, a survey was conducted to assess the occurrence of pathogenic yersinia enterocolitica in norwegian pork products, using a traditional culturing method and a pcr assay. a total of 300 pork samples was examined. five slaughterhouses in the norwegian meat cooperative were represented with 249 samples and another 51 samples were obtained from retail outlets in the city of oslo. using the nmkl method, y. enterocolitica 0:3 was isolated from six (2%) of the samples, wh ...200010746576
comparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of yersinia enterocolitica in swine and pork products.bacteriological culture was compared with multiplex and fluorogenic (taqman) polymerase chain reaction (pcr) assays for the detection of attachment invasion locus (ail)-bearing yersinia enterocolitica in market weight swine, chitterlings, and ground pork. the taqman assay detected 1 pg of purified y. enterocolitica dna, whereas conventional gel-based pcr detected i ng of the same. the presence of ail-bearing y. enterocolitica was tested in pork and feces artificially inoculated with y. enterocol ...200111563512
a comparison of sample preparation methods for pcr detection of pathogenic yersinia enterocolitica from ground pork using swabbing and slurry homogenate techniques.two sample preparation methods for multiplex polymerase chain reaction (pcr) for detection of plasmid-bearing virulent yersinia enterocolitica (yep(+)) from ground pork were compared. two sets of ground pork samples were inoculated with 10, 1, and 0.5 cfu/cm(2) of a yep(+) strain, one set was swabbed and the second set was dispersed into a slurry homogenate. both swab and slurry homogenate samples were enriched in sterile whirl pak bags containing modified trypticase soy broth for 48 h at 12 deg ...200312788031
typing of yersinia enterocolitica isolates by its profiling, rep- and eric-pcr.thirty-five yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (its) profiling, rep- and eric-pcr. eric-pcr revealed the presence of seven different genotypes. amplification of the 16s-23s rdna spacer region by its profiling gave similar results with nine different genotypes. rep-pcr was found to be more discriminatory for typing of y. enterocolitica than eric-pcr and its profiling. fifteen different dna patt ...200415330984
presence of yersinia enterocolitica in tissues of orally-inoculated pigs and the tonsils and feces of pigs at order to study the early events associated with infection of swine by yersinia enterocolitica, 42 five-week-old crossbred piglets were inoculated per os with approximately 10(8) y. enterocolitica o:3. groups of 5 animals (and one negative control) were euthanized 30 min, 3, 6, 12, 24, 48 and 72 h following the infection. palatine tonsils, retropharyngeal and mesenteric lymph nodes, esophagus, duodenum, jejunum, ileum (and peyer's patches), stomach, liver, spleen and feces (from colon) were co ...199910369565
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