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development and validation of a quantitative pcr for rapid and specific detection of california sea lion adenovirus 1 and prevalence in wild and managed populations.california sea lion adenovirus 1 (csladv-1) has been associated with hepatitis and enteritis in several wild and captive populations of diverse pinniped species. currently available tests have been limited to pan-adenoviral polymerase chain reaction (pcr) followed by sequencing. we present the development of a quantitative probe-hybridization pcr (qpcr) assay for rapid, sensitive, and specific detection of this virus in california sea lions ( zalophus californianus) and other pinnipeds. this ass ...201728166696
coinfection of california sea lion adenovirus 1 and a novel polyomavirus in a hawaiian monk seal (neomonachus schauinslandi).the hawaiian monk seal (neomonachus schauinslandi) is an endangered species. here, we present a clinical case of a 26-yr-old male hawaiian monk seal (hms) kept in an aquarium with a history of intermittent anorexia and evidence of renal disease. histologic examination revealed eosinophilic intranuclear inclusions in the liver. conventional nested pcr protocols were used to test for viruses, and it tested positive for adenovirus and polyomavirus, and negative for herpesvirus. the adenovirus parti ...201627468013
phylogenomic characterization of california sea lion adenovirus-1.significant adenoviral diversity has been found in humans, but in domestic and wild animals the number of identified viruses is lower. here we present the complete genome of a recently discovered mastadenovirus, california sea lion adenovirus 1 (csladv-1) isolated from california sea lions (zalophus californianus), an important pathogen associated with hepatitis in pinnipeds. the genome of this virus has the typical mastadenoviral structure with some notable differences at the carboxy-terminal e ...201525660039
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